Restriction map for
pF1KB5798
(Circular) (Six-base) MAPSORT of: pF1KB5798.seq Check: 545 from: 1 to: 5456
pF1KB5798 5456 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1500 1500
Size: 5456
AccI GT'mk_AC
Cuts at: 2494 2494
Size: 5456
Acc65I G'GTAC_C
Cuts at: 1081 2472 1081
Size: 1391 4065
AclI AA'CG_TT
Cuts at: 5296 5296
Size: 5456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1000 1264 1417 1493 1501 1978 2358 3281
Size: 519 264 153 76 8 477 380 923
Cuts at: 3281 3713 4460 4728 481
Size: 432 747 268 1209
Fragments arranged by size:
1209 923 747 519 477 432 380 268
264 153 76 8
AfeI AGC'GCT
Cuts at: 1478 1478
Size: 5456
AflIII A'CryG_T
Cuts at: 2386 2808 3912 4889 2386
Size: 422 1104 977 2953
Fragments arranged by size:
2953 1104 977 422
AgeI A'CCGG_T
Cuts at: 3844 3844
Size: 5456
AhdI GACnn_n'nnGTC
Cuts at: 2423 2423
Size: 5456
AleI CACnn'nnGTG
Cuts at: 202 202
Size: 5456
AlwNI CAG_nnn'CTG
Cuts at: 327 348 753 1123 1221 1732 2761 4328 327
Size: 21 405 370 98 511 1029 1567 1455
Fragments arranged by size:
1567 1455 1029 511 405 370 98 21
ApaI G_GGCC'C
Cuts at: 2360 2360
Size: 5456
ApaLI G'TGCA_C
Cuts at: 4226 4226
Size: 5456
ApoI r'AATT_y
Cuts at: 881 1373 2460 4809 5065 881
Size: 492 1087 2349 256 1272
Fragments arranged by size:
2349 1272 1087 492 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5456
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
AvaI C'yCGr_G
Cuts at: 478 547 768 810 938 1111 1197 2476 478
Size: 69 221 42 128 173 86 1279 3458
Fragments arranged by size:
3458 1279 221 173 128 86 69 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1073 1106 1595 1628 2007 2040 1073
Size: 33 489 33 379 33 4489
Fragments arranged by size:
4489 489 379 33 33 33
BamHI G'GATC_C
Cuts at: 224 2102 2481 224
Size: 1878 379 3199
Fragments arranged by size:
3199 1878 379
BanI G'GyrC_C
Cuts at: 156 763 864 1081 1127 1169 2472 2886 3134
Size: 607 101 217 46 42 1303 414 248
Cuts at: 3134 3169 156
Size: 35 2443
Fragments arranged by size:
2443 1303 607 414 248 217 101 46
42 35
BanII G_rGCy'C
Cuts at: 365 646 1198 1286 2041 2360 2470 3500 365
Size: 281 552 88 755 319 110 1030 2321
Fragments arranged by size:
2321 1030 755 552 319 281 110 88
BbeI G_GCGC'C
Cuts at: 767 868 2890 3138 767
Size: 101 2022 248 3085
Fragments arranged by size:
3085 2022 248 101
BbsI GAAGACnn'nnnn_
Cuts at: 469 469
Size: 5456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 774 808 2214 2248 774
Size: 34 1406 34 3982
Fragments arranged by size:
3982 1406 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1623 3354 4115 5456 1623
Size: 1731 761 1341 1623
Fragments arranged by size:
1731 1623 1341 761
BclI T'GATC_A
Cuts at: 2976 2976
Size: 5456
BglII A'GATC_T
Cuts at: 2971 5008 2971
Size: 2037 3419
BlpI GC'TnA_GC
Cuts at: 2568 2568
Size: 5456
Bme1580I G_kGCm'C
Cuts at: 161 1130 2360 3081 3174 4230 161
Size: 969 1230 721 93 1056 1387
Fragments arranged by size:
1387 1230 1056 969 721 93
BmgBI CAC'GTC
Cuts at: 1566 1566
Size: 5456
BmrI ACTGGGnnnn_n'
Cuts at: 1066 2079 2181 2668 2749 3084 5228 1066
Size: 1013 102 487 81 335 2144 1294
Fragments arranged by size:
2144 1294 1013 487 335 102 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 618 3823 618
Size: 3205 2251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 204 452 1408 1525 1546 1579 1675 166
Size: 38 248 956 117 21 33 96 3947
Fragments arranged by size:
3947 956 248 117 96 38 33 21
Bpu10I CC'TnA_GC
Cuts at: 580 1207 1886 580
Size: 627 679 4150
Fragments arranged by size:
4150 679 627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1742 2632 3511 4003 4301 856
Size: 886 890 879 492 298 2011
Fragments arranged by size:
2011 890 886 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1061 5160 1061
Size: 4099 1357
BsaAI yAC'GTr
Cuts at: 2389 2809 2389
Size: 420 5036
BsaBI GATnn'nnATC
Cuts at: 6 2970 5007 6
Size: 2964 2037 455
Fragments arranged by size:
2964 2037 455
BsaHI Gr'CG_yC
Cuts at: 764 865 2209 2887 3135 3837 5333 764
Size: 101 1344 678 248 702 1496 887
Fragments arranged by size:
1496 1344 887 702 678 248 101
BsaWI w'CCGG_w
Cuts at: 954 1015 2645 3166 3844 4118 4265 4676 954
Size: 61 1630 521 678 274 147 411 1734
Fragments arranged by size:
1734 1630 678 521 411 274 147 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 715 745 1058 1088 1498 1528 1657 1687 715
Size: 30 313 30 410 30 129 30 4484
Fragments arranged by size:
4484 410 313 129 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 373 1360 1435 1456 1745 1774 2402 163
Size: 210 987 75 21 289 29 628 3217
Fragments arranged by size:
3217 987 628 289 210 75 29 21
BseYI C'CCAG_C
Cuts at: 1214 2881 3639 4216 4712 1214
Size: 1667 758 577 496 1958
Fragments arranged by size:
1958 1667 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 328 558 804 1513 226
Size: 102 230 246 709 4169
Fragments arranged by size:
4169 709 246 230 102
BsiEI CG_ry'CG
Cuts at: 104 508 2998 3044 4252 104
Size: 404 2490 46 1208 1308
Fragments arranged by size:
2490 1308 1208 404 46
BsiHKAI G_wGCw'C
Cuts at: 218 365 1319 1819 2470 3248 3438 4230 218
Size: 147 954 500 651 778 190 792 1444
Fragments arranged by size:
1444 954 792 778 651 500 190 147
BsiWI C'GTAC_G
Cuts at: 509 509
Size: 5456
Bsp1286I G_dGCh'C
Cuts at: 161 218 365 646 1130 1198 1286 1319 1819
Size: 57 147 281 484 68 88 33 500
Cuts at: 1819 2041 2360 2470 3081 3174 3248 3438 3500
Size: 222 319 110 611 93 74 190 62
Cuts at: 3500 4230 161
Size: 730 1387
Fragments arranged by size:
1387 730 611 500 484 319 281 222
190 147 110 93 88 74 68 62
57 33
BspEI T'CCGG_A
Cuts at: 954 4676 954
Size: 3722 1734
BspHI T'CATG_A
Cuts at: 4632 4632
Size: 5456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 312 812 1239 1500 2506 2720 3022 3403 312
Size: 500 427 261 1006 214 302 381 2365
Fragments arranged by size:
2365 1006 500 427 381 302 261 214
BsrBI CCG'CTC
Cuts at: 50 1151 3748 3802 5286 5451 50
Size: 1101 2597 54 1484 165 55
Fragments arranged by size:
2597 1484 1101 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3368 3368
Size: 5456
BsrFI r'CCGG_y
Cuts at: 255 3454 3635 3844 255
Size: 3199 181 209 1867
Fragments arranged by size:
3199 1867 209 181
BssSI C'ACGA_G
Cuts at: 3727 4085 3727
Size: 358 5098
BstAPI GCAn_nnn'nTGC
Cuts at: 327 1491 2833 327
Size: 1164 1342 2950
Fragments arranged by size:
2950 1342 1164
BstBI TT'CG_AA
Cuts at: 794 3817 794
Size: 3023 2433
BstEII G'GTnAC_C
Cuts at: 279 840 1011 279
Size: 561 171 4724
Fragments arranged by size:
4724 561 171
BstXI CCAn_nnnn'nTGG
Cuts at: 276 435 1579 2259 276
Size: 159 1144 680 3473
Fragments arranged by size:
3473 1144 680 159
BstYI r'GATC_y
Cuts at: 1 224 239 1775 2102 2317 2481 2948 2971
Size: 223 15 1536 327 215 164 467 23
Cuts at: 2971 3306 3552 4564 4650 4662 5008 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1536 1012 467 449 346 335 327 246
223 215 164 86 23 15 12
Bsu36I CC'TnA_GG
Cuts at: 1140 1140
Size: 5456
BtgI C'CryG_G
Cuts at: 107 734 107
Size: 627 4829
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1608 3406 3587 3750 1608
Size: 1798 181 163 3314
Fragments arranged by size:
3314 1798 181 163
BtsI GCAGTG_nn'
Cuts at: 1457 2699 1457
Size: 1242 4214
DraI TTT'AAA
Cuts at: 2455 2455
Size: 5456
DraIII CAC_nnn'GTG
Cuts at: 1332 1332
Size: 5456
DrdI GACnn_nn'nnGTC
Cuts at: 3162 4020 3162
Size: 858 4598
EaeI y'GGCC_r
Cuts at: 266 353 505 2058 3041 3215 3606 3633 4978
Size: 87 152 1553 983 174 391 27 1345
Cuts at: 4978 266
Size: 744
Fragments arranged by size:
1553 1345 983 744 391 174 152 87
27
EagI C'GGCC_G
Cuts at: 505 3041 505
Size: 2536 2920
EarI CTCTTCn'nnn_
Cuts at: 290 1186 1267 1807 1954 3479 3689 290
Size: 896 81 540 147 1525 210 2057
Fragments arranged by size:
2057 1525 896 540 210 147 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 533 3664 3974 4120 5266 533
Size: 3131 310 146 1146 723
Fragments arranged by size:
3131 1146 723 310 146
EcoICRI GAG'CTC
Cuts at: 363 2468 363
Size: 2105 3351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 204 452 481 1000 1264 1408 1417 1493
Size: 38 248 29 519 264 144 9 76
Cuts at: 1493 1501 1525 1546 1579 1675 1978 2358 3281
Size: 8 24 21 33 96 303 380 923
Cuts at: 3281 3713 4460 4728 166
Size: 432 747 268 894
Fragments arranged by size:
923 894 747 519 432 380 303 268
264 248 144 96 76 38 33 29
24 21 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1246 1246
Size: 5456
EcoO109I rG'GnC_Cy
Cuts at: 324 609 788 988 2357 2443 2595 324
Size: 285 179 200 1369 86 152 3185
Fragments arranged by size:
3185 1369 285 200 179 152 86
EcoRI G'AATT_C
Cuts at: 2460 2460
Size: 5456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3475 3475
Size: 5456
FspI TGC'GCA
Cuts at: 718 3237 718
Size: 2519 2937
HaeII r_GCGC'y
Cuts at: 348 688 767 868 1239 1480 1886 2890 3138
Size: 340 79 101 371 241 406 1004 248
Cuts at: 3138 4160 5139 348
Size: 1022 979 665
Fragments arranged by size:
1022 1004 979 665 406 371 340 248
241 101 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 255 287 1414 1446 1657 1689 1740 1772 1869
Size: 32 1127 32 211 32 51 32 97
Cuts at: 1869 1901 2309 2341 2409 2441 3298 3328 3330
Size: 32 408 32 68 32 857 30 2
Cuts at: 3330 3360 255
Size: 30 2351
Fragments arranged by size:
2351 1127 857 408 211 97 68 51
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1077 2495 1077
Size: 1418 4038
KasI G'GCGC_C
Cuts at: 763 864 2886 3134 763
Size: 101 2022 248 3085
Fragments arranged by size:
3085 2022 248 101
KpnI G_GTAC'C
Cuts at: 1085 2476 1085
Size: 1391 4065
MluI A'CGCG_T
Cuts at: 4889 4889
Size: 5456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1803 2064 2265 4127 4311 1803
Size: 261 201 1862 184 2948
Fragments arranged by size:
2948 1862 261 201 184
MscI TGG'CCA
Cuts at: 268 3217 268
Size: 2949 2507
MslI CAynn'nnrTG
Cuts at: 202 2269 3445 3572 202
Size: 2067 1176 127 2086
Fragments arranged by size:
2086 2067 1176 127
MspA1I CmG'CkG
Cuts at: 736 910 1049 1257 1386 1488 2021 2342 2567
Size: 174 139 208 129 102 533 321 225
Cuts at: 2567 2881 3241 4254 4499 736
Size: 314 360 1013 245 1693
Fragments arranged by size:
1693 1013 533 360 321 314 245 225
208 174 139 129 102
NaeI GCC'GGC
Cuts at: 3637 3637
Size: 5456
NarI GG'CG_CC
Cuts at: 764 865 2887 3135 764
Size: 101 2022 248 3085
Fragments arranged by size:
3085 2022 248 101
NcoI C'CATG_G
Cuts at: 107 107
Size: 5456
NdeI CA'TA_TG
Cuts at: 1599 1599
Size: 5456
NgoMIV G'CCGG_C
Cuts at: 3635 3635
Size: 5456
NspI r_CATG'y
Cuts at: 1897 2509 3009 3916 1897
Size: 612 500 907 3437
Fragments arranged by size:
3437 907 612 500
PasI CC'CwG_GG
Cuts at: 435 2380 435
Size: 1945 3511
PciI A'CATG_T
Cuts at: 3912 3912
Size: 5456
PflMI CCAn_nnn'nTGG
Cuts at: 2164 2434 2164
Size: 270 5186
PfoI T'CCnGG_A
Cuts at: 220 1523 220
Size: 1303 4153
PmeI GTTT'AAAC
Cuts at: 2455 2455
Size: 5456
PmlI CAC'GTG
Cuts at: 2389 2389
Size: 5456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 4595
Size: 32 5424
PpuMI rG'GwC_Cy
Cuts at: 324 324
Size: 5456
PspOMI G'GGCC_C
Cuts at: 2356 2356
Size: 5456
PstI C_TGCA'G
Cuts at: 194 323 682 749 1105 1303 1694 2503 3188
Size: 129 359 67 356 198 391 809 685
Cuts at: 3188 194
Size: 2462
Fragments arranged by size:
2462 809 685 391 359 356 198 129
67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5456
PvuII CAG'CTG
Cuts at: 910 1257 1386 2021 2342 2881 3241 910
Size: 347 129 635 321 539 360 3125
Fragments arranged by size:
3125 635 539 360 347 321 129
RsrII CG'GwC_CG
Cuts at: 3651 3651
Size: 5456
SacI G_AGCT'C
Cuts at: 365 2470 365
Size: 2105 3351
SacII CC_GC'GG
Cuts at: 737 737
Size: 5456
SalI G'TCGA_C
Cuts at: 2493 2493
Size: 5456
SapI GCTCTTCn'nnn_
Cuts at: 1186 1267 1807 3479 3689 1186
Size: 81 540 1672 210 2953
Fragments arranged by size:
2953 1672 540 210 81
SbfI CC_TGCA'GG
Cuts at: 323 682 2503 323
Size: 359 1821 3276
Fragments arranged by size:
3276 1821 359
ScaI AGT'ACT
Cuts at: 1825 4973 1825
Size: 3148 2308
SfcI C'TryA_G
Cuts at: 33 190 319 678 745 851 1101 1299 1690
Size: 157 129 359 67 106 250 198 391
Cuts at: 1690 2277 2499 3184 4177 4368 33
Size: 587 222 685 993 191 1121
Fragments arranged by size:
1121 993 685 587 391 359 250 222
198 191 157 129 106 67
SfoI GGC'GCC
Cuts at: 765 866 2888 3136 765
Size: 101 2022 248 3085
Fragments arranged by size:
3085 2022 248 101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
SmaI CCC'GGG
Cuts at: 770 940 1199 2478 770
Size: 170 259 1279 3748
Fragments arranged by size:
3748 1279 259 170
SmlI C'TyrA_G
Cuts at: 835 1757 2611 3526 4018 4280 835
Size: 922 854 915 492 262 2011
Fragments arranged by size:
2011 922 915 854 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5456
SphI G_CATG'C
Cuts at: 2509 3009 2509
Size: 500 4956
SspI AAT'ATT
Cuts at: 388 388
Size: 5456
StuI AGG'CCT
Cuts at: 601 1138 601
Size: 537 4919
StyI C'CwwG_G
Cuts at: 107 1320 1661 2590 2944 107
Size: 1213 341 929 354 2619
Fragments arranged by size:
2619 1213 929 354 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 999 2163 2422 3039 3172 3631 3840 999
Size: 1164 259 617 133 459 209 2615
Fragments arranged by size:
2615 1164 617 459 259 209 133
TatI w'GTAC_w
Cuts at: 1514 1823 4971 1514
Size: 309 3148 1999
Fragments arranged by size:
3148 1999 309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4973 5005 4973
Size: 32 5424
Tth111I GACn'n_nGTC
Cuts at: 2151 3253 2151
Size: 1102 4354
XbaI T'CTAG_A
Cuts at: 894 2487 894
Size: 1593 3863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2951 2951
Size: 5456
XmaI C'CCGG_G
Cuts at: 768 938 1197 2476 768
Size: 170 259 1279 3748
Fragments arranged by size:
3748 1279 259 170
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI AvrII BbvCI BfrBI
BglI BmtI BsmI BsmBI BsrGI BssHII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI