Restriction map for
pF1KB5799
(Circular) (Six-base) MAPSORT of: pF1KB5799.seq Check: 8901 from: 1 to: 5615
pF1KB5799 5615 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 977 1700 977
Size: 723 4892
AccI GT'mk_AC
Cuts at: 1991 2653 1991
Size: 662 4953
Acc65I G'GTAC_C
Cuts at: 2631 2631
Size: 5615
AclI AA'CG_TT
Cuts at: 2564 5455 2564
Size: 2891 2724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 871 3440 3872 4619 4887 688
Size: 183 2569 432 747 268 1416
Fragments arranged by size:
2569 1416 747 432 268 183
AflIII A'CryG_T
Cuts at: 931 2967 4071 5048 931
Size: 2036 1104 977 1498
Fragments arranged by size:
2036 1498 1104 977
AgeI A'CCGG_T
Cuts at: 4003 4003
Size: 5615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1975 1975
Size: 5615
AlwNI CAG_nnn'CTG
Cuts at: 1005 1023 1103 1644 2106 2920 4487 1005
Size: 18 80 541 462 814 1567 2133
Fragments arranged by size:
2133 1567 814 541 462 80 18
ApaI G_GGCC'C
Cuts at: 205 2273 205
Size: 2068 3547
ApaLI G'TGCA_C
Cuts at: 4385 4385
Size: 5615
ApoI r'AATT_y
Cuts at: 276 745 2009 2122 2619 4968 5224 276
Size: 469 1264 113 497 2349 256 667
Fragments arranged by size:
2349 1264 667 497 469 256 113
AseI AT'TA_AT
Cuts at: 20 1496 20
Size: 1476 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
AvaI C'yCGr_G
Cuts at: 1667 1724 2635 1667
Size: 57 911 4647
Fragments arranged by size:
4647 911 57
BamHI G'GATC_C
Cuts at: 2640 2640
Size: 5615
BanI G'GyrC_C
Cuts at: 2631 3045 3293 3328 2631
Size: 414 248 35 4918
Fragments arranged by size:
4918 414 248 35
BanII G_rGCy'C
Cuts at: 205 863 959 2156 2273 2629 3659 205
Size: 658 96 1197 117 356 1030 2161
Fragments arranged by size:
2161 1197 1030 658 356 117 96
BbeI G_GCGC'C
Cuts at: 3049 3297 3049
Size: 248 5367
BbsI GAAGACnn'nnnn_
Cuts at: 713 1338 2316 713
Size: 625 978 4012
Fragments arranged by size:
4012 978 625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2332 2366 2332
Size: 34 5581
BciVI GTATCCnnnnn_n'
Cuts at: 277 3513 4274 5615 277
Size: 3236 761 1341 277
Fragments arranged by size:
3236 1341 761 277
BclI T'GATC_A
Cuts at: 1928 3135 1928
Size: 1207 4408
BfrBI ATG'CAT
Cuts at: 1570 1570
Size: 5615
BglI GCCn_nnn'nGGC
Cuts at: 2268 2268
Size: 5615
BglII A'GATC_T
Cuts at: 3130 5167 3130
Size: 2037 3578
BlpI GC'TnA_GC
Cuts at: 862 2727 862
Size: 1865 3750
Bme1580I G_kGCm'C
Cuts at: 205 1137 1551 1818 1832 2273 3240 3333 4389
Size: 932 414 267 14 441 967 93 1056
Cuts at: 4389 205
Size: 1431
Fragments arranged by size:
1431 1056 967 932 441 414 267 93
14
BmgBI CAC'GTC
Cuts at: 934 934
Size: 5615
BmrI ACTGGGnnnn_n'
Cuts at: 187 393 1343 2827 2908 3243 5387 187
Size: 206 950 1484 81 335 2144 415
Fragments arranged by size:
2144 1484 950 415 335 206 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 833 3982 833
Size: 3149 2466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 547 1402 355
Size: 192 855 4568
Fragments arranged by size:
4568 855 192
Bpu10I CC'TnA_GC
Cuts at: 916 916
Size: 5615
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1725 2791 3670 4162 4460 346
Size: 1379 1066 879 492 298 1501
Fragments arranged by size:
1501 1379 1066 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 147 2290 2433 5319 147
Size: 2143 143 2886 443
Fragments arranged by size:
2886 2143 443 143
BsaAI yAC'GTr
Cuts at: 2194 2968 2194
Size: 774 4841
BsaBI GATnn'nnATC
Cuts at: 6 3129 5166 6
Size: 3123 2037 455
Fragments arranged by size:
3123 2037 455
BsaHI Gr'CG_yC
Cuts at: 578 974 1697 1994 3046 3294 3996 5492 578
Size: 396 723 297 1052 248 702 1496 701
Fragments arranged by size:
1496 1052 723 702 701 396 297 248
BsaWI w'CCGG_w
Cuts at: 2804 3325 4003 4277 4424 4835 2804
Size: 521 678 274 147 411 3584
Fragments arranged by size:
3584 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 530 560 1669 1699 530
Size: 30 1109 30 4446
Fragments arranged by size:
4446 1109 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 159 232 235 247 250 253 256 628 1540
Size: 73 3 12 3 3 3 372 912
Cuts at: 1540 1984 159
Size: 444 3790
Fragments arranged by size:
3790 912 444 372 73 12 3 3
3 3
BseYI C'CCAG_C
Cuts at: 181 1575 2389 3040 3798 4375 4871 181
Size: 1394 814 651 758 577 496 925
Fragments arranged by size:
1394 925 814 758 651 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 1519 1570 2081 2299 1204
Size: 315 51 511 218 4520
Fragments arranged by size:
4520 511 315 218 51
BsiEI CG_ry'CG
Cuts at: 104 3157 3203 4411 104
Size: 3053 46 1208 1308
Fragments arranged by size:
3053 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 473 654 959 2177 2629 3407 3597 4389 473
Size: 181 305 1218 452 778 190 792 1699
Fragments arranged by size:
1699 1218 792 778 452 305 190 181
BsmBI CGTCTCn'nnnn_
Cuts at: 1603 1603
Size: 5615
Bsp1286I G_dGCh'C
Cuts at: 205 473 654 863 959 1137 1551 1818 1832
Size: 268 181 209 96 178 414 267 14
Cuts at: 1832 2156 2177 2273 2629 3240 3333 3407 3597
Size: 324 21 96 356 611 93 74 190
Cuts at: 3597 3659 4389 205
Size: 62 730 1431
Fragments arranged by size:
1431 730 611 414 356 324 268 267
209 190 181 178 96 96 93 74
62 21 14
BspEI T'CCGG_A
Cuts at: 4835 4835
Size: 5615
BspHI T'CATG_A
Cuts at: 942 2051 2570 4791 942
Size: 1109 519 2221 1766
Fragments arranged by size:
2221 1766 1109 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1098 1226 1592 2665 2879 3181 3562 1098
Size: 128 366 1073 214 302 381 3151
Fragments arranged by size:
3151 1073 381 366 302 214 128
BsrBI CCG'CTC
Cuts at: 50 1160 3907 3961 5445 5610 50
Size: 1110 2747 54 1484 165 55
Fragments arranged by size:
2747 1484 1110 165 55 54
BsrDI GCAATG_nn'
Cuts at: 293 1193 1570 3527 293
Size: 900 377 1957 2381
Fragments arranged by size:
2381 1957 900 377
BsrFI r'CCGG_y
Cuts at: 3613 3794 4003 3613
Size: 181 209 5225
Fragments arranged by size:
5225 209 181
BsrGI T'GTAC_A
Cuts at: 830 1127 830
Size: 297 5318
BssSI C'ACGA_G
Cuts at: 352 1649 2414 3886 4244 352
Size: 1297 765 1472 358 1723
Fragments arranged by size:
1723 1472 1297 765 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2992 2992
Size: 5615
BstBI TT'CG_AA
Cuts at: 3976 3976
Size: 5615
BstXI CCAn_nnnn'nTGG
Cuts at: 1563 2145 1563
Size: 582 5033
BstYI r'GATC_y
Cuts at: 1 254 1305 1595 2544 2640 3107 3130 3465
Size: 253 1051 290 949 96 467 23 335
Cuts at: 3465 3711 4723 4809 4821 5167 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1051 1012 949 467 449 346 335 290
253 246 96 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 117 1144 117
Size: 1027 4588
BtgI C'CryG_G
Cuts at: 107 1469 2291 107
Size: 1362 822 3431
Fragments arranged by size:
3431 1362 822
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3565 3746 3909 3565
Size: 181 163 5271
Fragments arranged by size:
5271 181 163
BtsI GCAGTG_nn'
Cuts at: 1419 1587 2087 2093 2451 2858 1419
Size: 168 500 6 358 407 4176
Fragments arranged by size:
4176 500 407 358 168 6
DraI TTT'AAA
Cuts at: 2614 2614
Size: 5615
DrdI GACnn_nn'nnGTC
Cuts at: 715 3321 4179 715
Size: 2606 858 2151
Fragments arranged by size:
2606 2151 858
EaeI y'GGCC_r
Cuts at: 248 839 2165 2222 3200 3374 3765 3792 5137
Size: 591 1326 57 978 174 391 27 1345
Cuts at: 5137 248
Size: 726
Fragments arranged by size:
1345 1326 978 726 591 391 174 57
27
EagI C'GGCC_G
Cuts at: 3200 3200
Size: 5615
EarI CTCTTCn'nnn_
Cuts at: 610 618 1894 3638 3848 610
Size: 8 1276 1744 210 2377
Fragments arranged by size:
2377 1744 1276 210 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3823 4133 4279 5425 3823
Size: 310 146 1146 4013
Fragments arranged by size:
4013 1146 310 146
EcoICRI GAG'CTC
Cuts at: 957 2627 957
Size: 1670 3945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 547 688 871 1402 3440 3872 4619 4887
Size: 192 141 183 531 2038 432 747 268
Cuts at: 4887 355
Size: 1083
Fragments arranged by size:
2038 1083 747 531 432 268 192 183
141
EcoNI CCTnn'n_nnAGG
Cuts at: 1360 1915 2065 1360
Size: 555 150 4910
Fragments arranged by size:
4910 555 150
EcoO109I rG'GnC_Cy
Cuts at: 202 1581 2269 2270 2754 202
Size: 1379 688 1 484 3063
Fragments arranged by size:
3063 1379 688 484 1
EcoRI G'AATT_C
Cuts at: 276 2009 2619 276
Size: 1733 610 3272
Fragments arranged by size:
3272 1733 610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3634 3634
Size: 5615
FspI TGC'GCA
Cuts at: 3396 3396
Size: 5615
HaeII r_GCGC'y
Cuts at: 3049 3297 4319 5298 3049
Size: 248 1022 979 3366
Fragments arranged by size:
3366 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 479 511 1011 1043 1643 1669 1675 1701 3457
Size: 32 500 32 600 26 6 26 1756
Cuts at: 3457 3487 3489 3519 479
Size: 30 2 30 2575
Fragments arranged by size:
2575 1756 600 500 32 32 30 30
26 26 6 2
HincII GTy'rAC
Cuts at: 755 2654 755
Size: 1899 3716
HindIII A'AGCT_T
Cuts at: 364 454 2404 364
Size: 90 1950 3575
Fragments arranged by size:
3575 1950 90
KasI G'GCGC_C
Cuts at: 3045 3293 3045
Size: 248 5367
KpnI G_GTAC'C
Cuts at: 2635 2635
Size: 5615
MluI A'CGCG_T
Cuts at: 5048 5048
Size: 5615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 961 2202 4286 4470 961
Size: 1241 2084 184 2106
Fragments arranged by size:
2106 2084 1241 184
MscI TGG'CCA
Cuts at: 841 2224 3376 841
Size: 1383 1152 3080
Fragments arranged by size:
3080 1383 1152
MslI CAynn'nnrTG
Cuts at: 263 1936 2050 2218 3604 3731 263
Size: 1673 114 168 1386 127 2147
Fragments arranged by size:
2147 1673 1386 168 127 114
MspA1I CmG'CkG
Cuts at: 1657 2170 2380 2726 3040 3400 4413 4658 1657
Size: 513 210 346 314 360 1013 245 2614
Fragments arranged by size:
2614 1013 513 360 346 314 245 210
NaeI GCC'GGC
Cuts at: 3796 3796
Size: 5615
NarI GG'CG_CC
Cuts at: 3046 3294 3046
Size: 248 5367
NcoI C'CATG_G
Cuts at: 107 1469 2291 107
Size: 1362 822 3431
Fragments arranged by size:
3431 1362 822
NgoMIV G'CCGG_C
Cuts at: 3794 3794
Size: 5615
NsiI A_TGCA'T
Cuts at: 1572 1572
Size: 5615
NspI r_CATG'y
Cuts at: 2207 2668 3168 4075 2207
Size: 461 500 907 3747
Fragments arranged by size:
3747 907 500 461
PasI CC'CwG_GG
Cuts at: 206 1137 206
Size: 931 4684
PciI A'CATG_T
Cuts at: 4071 4071
Size: 5615
PflMI CCAn_nnn'nTGG
Cuts at: 1005 1644 1005
Size: 639 4976
PfoI T'CCnGG_A
Cuts at: 1598 1598
Size: 5615
PmeI GTTT'AAAC
Cuts at: 2614 2614
Size: 5615
PmlI CAC'GTG
Cuts at: 2194 2194
Size: 5615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 4754
Size: 32 5583
PpuMI rG'GwC_Cy
Cuts at: 1581 1581
Size: 5615
PspOMI G'GGCC_C
Cuts at: 201 2269 201
Size: 2068 3547
PstI C_TGCA'G
Cuts at: 2662 3347 2662
Size: 685 4930
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5615
PvuII CAG'CTG
Cuts at: 1657 2380 3040 3400 1657
Size: 723 660 360 3872
Fragments arranged by size:
3872 723 660 360
RsrII CG'GwC_CG
Cuts at: 3810 3810
Size: 5615
SacI G_AGCT'C
Cuts at: 959 2629 959
Size: 1670 3945
SalI G'TCGA_C
Cuts at: 2652 2652
Size: 5615
SapI GCTCTTCn'nnn_
Cuts at: 1894 3638 3848 1894
Size: 1744 210 3661
Fragments arranged by size:
3661 1744 210
SbfI CC_TGCA'GG
Cuts at: 2662 2662
Size: 5615
ScaI AGT'ACT
Cuts at: 5132 5132
Size: 5615
SexAI A'CCwGG_T
Cuts at: 2066 2066
Size: 5615
SfcI C'TryA_G
Cuts at: 33 796 1630 2658 3343 4336 4527 33
Size: 763 834 1028 685 993 191 1121
Fragments arranged by size:
1121 1028 993 834 763 685 191
SfoI GGC'GCC
Cuts at: 3047 3295 3047
Size: 248 5367
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
SmaI CCC'GGG
Cuts at: 1726 2637 1726
Size: 911 4704
SmlI C'TyrA_G
Cuts at: 361 1740 2770 3685 4177 4439 361
Size: 1379 1030 915 492 262 1537
Fragments arranged by size:
1537 1379 1030 915 492 262
SpeI A'CTAG_T
Cuts at: 67 310 67
Size: 243 5372
SphI G_CATG'C
Cuts at: 2668 3168 2668
Size: 500 5115
StyI C'CwwG_G
Cuts at: 107 130 1091 1469 1556 1767 2034 2291 2749
Size: 23 961 378 87 211 267 257 458
Cuts at: 2749 3103 107
Size: 354 2619
Fragments arranged by size:
2619 961 458 378 354 267 257 211
87 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 165 3198 3331 3790 3999 165
Size: 3033 133 459 209 1781
Fragments arranged by size:
3033 1781 459 209 133
TatI w'GTAC_w
Cuts at: 830 1127 5130 830
Size: 297 4003 1315
Fragments arranged by size:
4003 1315 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5132 5164 5132
Size: 32 5583
Tth111I GACn'n_nGTC
Cuts at: 3412 3412
Size: 5615
XbaI T'CTAG_A
Cuts at: 2646 2646
Size: 5615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3110 3110
Size: 5615
XmaI C'CCGG_G
Cuts at: 1724 2635 1724
Size: 911 4704
XmnI GAAnn'nnTTC
Cuts at: 2566 2566
Size: 5615
ZraI GAC'GTC
Cuts at: 975 1698 975
Size: 723 4892
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BmtI BsiWI BsmI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI