Restriction map for
pF1KB5808
(Circular) (Six-base) MAPSORT of: pF1KB5808.seq Check: 2821 from: 1 to: 4475
pF1KB5808 4475 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 365 736 1513 365
Size: 371 777 3327
Fragments arranged by size:
3327 777 371
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 1477 2300 2732 3479 3747 1327
Size: 150 823 432 747 268 2055
Fragments arranged by size:
2055 823 747 432 268 150
AflIII A'CryG_T
Cuts at: 479 481 1229 1827 2931 3908 479
Size: 2 748 598 1104 977 1046
Fragments arranged by size:
1104 1046 977 748 598 2
AgeI A'CCGG_T
Cuts at: 2863 2863
Size: 4475
AlwNI CAG_nnn'CTG
Cuts at: 1062 1780 3347 1062
Size: 718 1567 2190
Fragments arranged by size:
2190 1567 718
ApaI G_GGCC'C
Cuts at: 1025 1025
Size: 4475
ApaLI G'TGCA_C
Cuts at: 3245 3245
Size: 4475
ApoI r'AATT_y
Cuts at: 1479 3828 4084 1479
Size: 2349 256 1870
Fragments arranged by size:
2349 1870 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 359 1495 359
Size: 1136 3339
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 142 327 1205 1491 1905 2153 2188 142
Size: 185 878 286 414 248 35 2429
Fragments arranged by size:
2429 878 414 286 248 185 35
BanII G_rGCy'C
Cuts at: 238 658 674 869 1025 1148 1489 2519 238
Size: 420 16 195 156 123 341 1030 2194
Fragments arranged by size:
2194 1030 420 341 195 156 123 16
BbeI G_GCGC'C
Cuts at: 1209 1909 2157 1209
Size: 700 248 3527
Fragments arranged by size:
3527 700 248
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3134 4475 2373
Size: 761 1341 2373
Fragments arranged by size:
2373 1341 761
BclI T'GATC_A
Cuts at: 1995 1995
Size: 4475
BglI GCCn_nnn'nGGC
Cuts at: 728 728
Size: 4475
BglII A'GATC_T
Cuts at: 1990 4027 1990
Size: 2037 2438
BlpI GC'TnA_GC
Cuts at: 232 652 868 1099 1587 232
Size: 420 216 231 488 3120
Fragments arranged by size:
3120 488 420 231 216
Bme1580I G_kGCm'C
Cuts at: 147 204 332 1025 2100 2193 3249 147
Size: 57 128 693 1075 93 1056 1373
Fragments arranged by size:
1373 1075 1056 693 128 93 57
BmrI ACTGGGnnnn_n'
Cuts at: 1687 1768 2103 4247 1687
Size: 81 335 2144 1915
Fragments arranged by size:
2144 1915 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 300 1398 2842 300
Size: 1098 1444 1933
Fragments arranged by size:
1933 1444 1098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 732 1090 1159 1375 732
Size: 358 69 216 3832
Fragments arranged by size:
3832 358 216 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1651 2530 3022 3320 1651
Size: 879 492 298 2806
Fragments arranged by size:
2806 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 218 1118 4179 218
Size: 900 3061 514
Fragments arranged by size:
3061 900 514
BsaAI yAC'GTr
Cuts at: 482 1828 482
Size: 1346 3129
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 1206 1906 2154 2856 4352 1206
Size: 700 248 702 1496 1329
Fragments arranged by size:
1496 1329 702 700 248
BsaWI w'CCGG_w
Cuts at: 596 992 1664 2185 2863 3137 3284 3695 596
Size: 396 672 521 678 274 147 411 1376
Fragments arranged by size:
1376 678 672 521 411 396 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 347 1156 1374 334
Size: 13 809 218 3435
Fragments arranged by size:
3435 809 218 13
BseYI C'CCAG_C
Cuts at: 331 726 1051 1216 1464 1900 2658 3235 3731
Size: 395 325 165 248 436 758 577 496
Cuts at: 3731 331
Size: 1075
Fragments arranged by size:
1075 758 577 496 436 395 325 248
165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 787 918 773
Size: 14 131 4330
Fragments arranged by size:
4330 131 14
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3271 104
Size: 1913 46 1208 1308
Fragments arranged by size:
1913 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 310 674 1489 2267 2457 3249 310
Size: 364 815 778 190 792 1536
Fragments arranged by size:
1536 815 792 778 364 190
Bsp1286I G_dGCh'C
Cuts at: 147 204 238 310 332 658 674 869 1025
Size: 57 34 72 22 326 16 195 156
Cuts at: 1025 1148 1489 2100 2193 2267 2457 2519 3249
Size: 123 341 611 93 74 190 62 730
Cuts at: 3249 147
Size: 1373
Fragments arranged by size:
1373 730 611 341 326 195 190 156
123 93 74 72 62 57 34 22
16
BspEI T'CCGG_A
Cuts at: 3695 3695
Size: 4475
BspHI T'CATG_A
Cuts at: 3651 3651
Size: 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 166 1525 1739 2041 2422 166
Size: 1359 214 302 381 2219
Fragments arranged by size:
2219 1359 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2767 2821 4305 4470 50
Size: 2717 54 1484 165 55
Fragments arranged by size:
2717 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 393 872 2387 393
Size: 479 1515 2481
Fragments arranged by size:
2481 1515 479
BsrFI r'CCGG_y
Cuts at: 542 1012 1208 2473 2654 2863 542
Size: 470 196 1265 181 209 2154
Fragments arranged by size:
2154 1265 470 209 196 181
BsrGI T'GTAC_A
Cuts at: 476 476
Size: 4475
BssSI C'ACGA_G
Cuts at: 2746 3104 2746
Size: 358 4117
BstAPI GCAn_nnn'nTGC
Cuts at: 1062 1852 1062
Size: 790 3685
BstBI TT'CG_AA
Cuts at: 560 2836 560
Size: 2276 2199
BstEII G'GTnAC_C
Cuts at: 516 632 516
Size: 116 4359
BstXI CCAn_nnnn'nTGG
Cuts at: 279 279
Size: 4475
BstYI r'GATC_y
Cuts at: 1 157 774 1500 1967 1990 2325 2571 3583
Size: 156 617 726 467 23 335 246 1012
Cuts at: 3583 3669 3681 4027 1
Size: 86 12 346 449
Fragments arranged by size:
1012 726 617 467 449 346 335 246
156 86 23 12
BstZ17I GTA'TAC
Cuts at: 737 737
Size: 4475
Bsu36I CC'TnA_GG
Cuts at: 1189 1189
Size: 4475
BtgI C'CryG_G
Cuts at: 107 107
Size: 4475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 1174 1718 1174
Size: 544 3931
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DraIII CAC_nnn'GTG
Cuts at: 765 765
Size: 4475
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3039 2181
Size: 858 3617
EaeI y'GGCC_r
Cuts at: 110 2060 2234 2625 2652 3997 110
Size: 1950 174 391 27 1345 588
Fragments arranged by size:
1950 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 662 2498 2708 662
Size: 1836 210 2429
Fragments arranged by size:
2429 1836 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 206 341 2683 2993 3139 4285 206
Size: 135 2342 310 146 1146 396
Fragments arranged by size:
2342 1146 396 310 146 135
EcoICRI GAG'CTC
Cuts at: 672 1487 672
Size: 815 3660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 732 1090 1159 1327 1375 1477 2300 2732 3479
Size: 358 69 168 48 102 823 432 747
Cuts at: 3479 3747 732
Size: 268 1460
Fragments arranged by size:
1460 823 747 432 358 268 168 102
69 48
EcoNI CCTnn'n_nnAGG
Cuts at: 271 271
Size: 4475
EcoO109I rG'GnC_Cy
Cuts at: 151 249 1022 1452 1614 151
Size: 98 773 430 162 3012
Fragments arranged by size:
3012 773 430 162 98
EcoRI G'AATT_C
Cuts at: 1479 1479
Size: 4475
EcoRV GAT'ATC
Cuts at: 915 915
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 393 462 2494 393
Size: 69 2032 2374
Fragments arranged by size:
2374 2032 69
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 1106 1209 1909 2157 3179 4158 1106
Size: 103 700 248 1022 979 1423
Fragments arranged by size:
1423 1022 979 700 248 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 270 302 382 414 510 542 1369 1401 2317
Size: 32 80 32 96 32 827 32 916
Cuts at: 2317 2347 2349 2379 270
Size: 30 2 30 2366
Fragments arranged by size:
2366 916 827 96 80 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1514 1514
Size: 4475
KasI G'GCGC_C
Cuts at: 1205 1905 2153 1205
Size: 700 248 3527
Fragments arranged by size:
3527 700 248
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 998 3146 3330 998
Size: 2148 184 2143
Fragments arranged by size:
2148 2143 184
MscI TGG'CCA
Cuts at: 112 2236 112
Size: 2124 2351
MslI CAynn'nnrTG
Cuts at: 1406 2464 2591 1406
Size: 1058 127 3290
Fragments arranged by size:
3290 1058 127
MspA1I CmG'CkG
Cuts at: 726 771 1586 1900 2260 3273 3518 726
Size: 45 815 314 360 1013 245 1683
Fragments arranged by size:
1683 1013 815 360 314 245 45
NaeI GCC'GGC
Cuts at: 544 1014 1210 2656 544
Size: 470 196 1446 2363
Fragments arranged by size:
2363 1446 470 196
NarI GG'CG_CC
Cuts at: 1206 1906 2154 1206
Size: 700 248 3527
Fragments arranged by size:
3527 700 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4475
NgoMIV G'CCGG_C
Cuts at: 542 1012 1208 2654 542
Size: 470 196 1446 2363
Fragments arranged by size:
2363 1446 470 196
NspI r_CATG'y
Cuts at: 573 1233 1528 2028 2935 573
Size: 660 295 500 907 2113
Fragments arranged by size:
2113 907 660 500 295
PasI CC'CwG_GG
Cuts at: 147 169 196 147
Size: 22 27 4426
Fragments arranged by size:
4426 27 22
PciI A'CATG_T
Cuts at: 1229 2931 1229
Size: 1702 2773
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PmlI CAC'GTG
Cuts at: 482 482
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4443
PspOMI G'GGCC_C
Cuts at: 1021 1021
Size: 4475
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 460 492 460
Size: 32 4443
PstI C_TGCA'G
Cuts at: 1169 1522 2207 1169
Size: 353 685 3437
Fragments arranged by size:
3437 685 353
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 726 771 1900 2260 726
Size: 45 1129 360 2941
Fragments arranged by size:
2941 1129 360 45
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 674 1489 674
Size: 815 3660
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 662 2498 2708 662
Size: 1836 210 2429
Fragments arranged by size:
2429 1836 210
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 538 3992 538
Size: 3454 1021
SfcI C'TryA_G
Cuts at: 33 547 1165 1518 2203 3196 3387 33
Size: 514 618 353 685 993 191 1121
Fragments arranged by size:
1121 993 685 618 514 353 191
SfoI GGC'GCC
Cuts at: 1207 1907 2155 1207
Size: 700 248 3527
Fragments arranged by size:
3527 700 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SgrAI Cr'CCGG_yG
Cuts at: 1208 1208
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 1630 2545 3037 3299 1630
Size: 915 492 262 2806
Fragments arranged by size:
2806 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
StyI C'CwwG_G
Cuts at: 107 272 575 680 1237 1609 1963 107
Size: 165 303 105 557 372 354 2619
Fragments arranged by size:
2619 557 372 354 303 165 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2058 2191 2650 2859 2058
Size: 133 459 209 3674
Fragments arranged by size:
3674 459 209 133
TatI w'GTAC_w
Cuts at: 476 536 3990 476
Size: 60 3454 961
Fragments arranged by size:
3454 961 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3992 4024 3992
Size: 32 4443
Tth111I GACn'n_nGTC
Cuts at: 1036 2272 1036
Size: 1236 3239
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1970 1970
Size: 4475
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
XmnI GAAnn'nnTTC
Cuts at: 556 556
Size: 4475
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PmlI PpiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII ClaI
CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PpuMI PshAI
PsiI PspXI SacII SanDI SexAI SfiI SnaBI SrfI
SspI StuI SwaI XhoI ZraI