Restriction map for
pF1KB5811
(Circular) (Six-base) MAPSORT of: pF1KB5811.seq Check: 3991 from: 1 to: 4535
pF1KB5811 4535 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 456 1240 456
Size: 784 3751
AccI GT'mk_AC
Cuts at: 409 1573 409
Size: 1164 3371
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 1138 1266 1385 2360 2792 3539 3807 660
Size: 478 128 119 975 432 747 268 1388
Fragments arranged by size:
1388 975 747 478 432 268 128 119
AfeI AGC'GCT
Cuts at: 883 883
Size: 4535
AflII C'TTAA_G
Cuts at: 1245 1245
Size: 4535
AflIII A'CryG_T
Cuts at: 1887 2991 3968 1887
Size: 1104 977 2454
Fragments arranged by size:
2454 1104 977
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AhdI GACnn_n'nnGTC
Cuts at: 1503 1503
Size: 4535
AleI CACnn'nnGTG
Cuts at: 1456 1456
Size: 4535
AlwNI CAG_nnn'CTG
Cuts at: 1840 3407 1840
Size: 1567 2968
ApaLI G'TGCA_C
Cuts at: 3305 3305
Size: 4535
ApoI r'AATT_y
Cuts at: 251 1539 3888 4144 251
Size: 1288 2349 256 642
Fragments arranged by size:
2349 1288 642 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 1484 1555 1484
Size: 71 4464
BamHI G'GATC_C
Cuts at: 1560 1560
Size: 4535
BanI G'GyrC_C
Cuts at: 1551 1965 2213 2248 1551
Size: 414 248 35 3838
Fragments arranged by size:
3838 414 248 35
BanII G_rGCy'C
Cuts at: 521 1549 2579 521
Size: 1028 1030 2477
Fragments arranged by size:
2477 1030 1028
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbsI GAAGACnn'nnnn_
Cuts at: 387 1240 387
Size: 853 3682
BciVI GTATCCnnnnn_n'
Cuts at: 2433 3194 4535 2433
Size: 761 1341 2433
Fragments arranged by size:
2433 1341 761
BclI T'GATC_A
Cuts at: 2055 2055
Size: 4535
BglII A'GATC_T
Cuts at: 803 2050 4087 803
Size: 1247 2037 1251
Fragments arranged by size:
2037 1251 1247
BlpI GC'TnA_GC
Cuts at: 1647 1647
Size: 4535
Bme1580I G_kGCm'C
Cuts at: 1028 1255 2160 2253 3309 1028
Size: 227 905 93 1056 2254
Fragments arranged by size:
2254 1056 905 227 93
BmrI ACTGGGnnnn_n'
Cuts at: 1043 1747 1828 2163 4307 1043
Size: 704 81 335 2144 1271
Fragments arranged by size:
2144 1271 704 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1446 1540 928
Size: 518 94 3923
Fragments arranged by size:
3923 518 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1711 2590 3082 3380 463
Size: 1248 879 492 298 1618
Fragments arranged by size:
1618 1248 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1408 4239 1408
Size: 2831 1704
BsaAI yAC'GTr
Cuts at: 1888 1888
Size: 4535
BsaBI GATnn'nnATC
Cuts at: 6 2049 4086 6
Size: 2043 2037 455
Fragments arranged by size:
2043 2037 455
BsaHI Gr'CG_yC
Cuts at: 453 1237 1966 2214 2916 4412 453
Size: 784 729 248 702 1496 576
Fragments arranged by size:
1496 784 729 702 576 248
BsaWI w'CCGG_w
Cuts at: 1724 2245 2923 3197 3344 3755 1724
Size: 521 678 274 147 411 2504
Fragments arranged by size:
2504 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 577 607 631 661 577
Size: 30 24 30 4451
Fragments arranged by size:
4451 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 892 933 652
Size: 240 41 4254
Fragments arranged by size:
4254 240 41
BseYI C'CCAG_C
Cuts at: 457 660 1071 1960 2718 3295 3791 457
Size: 203 411 889 758 577 496 1201
Fragments arranged by size:
1201 889 758 577 496 411 203
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 655 682 195
Size: 460 27 4048
Fragments arranged by size:
4048 460 27
BsiEI CG_ry'CG
Cuts at: 104 2077 2123 3331 104
Size: 1973 46 1208 1308
Fragments arranged by size:
1973 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 539 1549 2327 2517 3309 539
Size: 1010 778 190 792 1765
Fragments arranged by size:
1765 1010 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 521 539 1028 1255 1549 2160 2253 2327 2517
Size: 18 489 227 294 611 93 74 190
Cuts at: 2517 2579 3309 521
Size: 62 730 1747
Fragments arranged by size:
1747 730 611 489 294 227 190 93
74 62 18
BspEI T'CCGG_A
Cuts at: 3755 3755
Size: 4535
BspHI T'CATG_A
Cuts at: 3711 3711
Size: 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 193 701 746 1585 1799 2101 2482 193
Size: 508 45 839 214 302 381 2246
Fragments arranged by size:
2246 839 508 381 302 214 45
BsrBI CCG'CTC
Cuts at: 50 2827 2881 4365 4530 50
Size: 2777 54 1484 165 55
Fragments arranged by size:
2777 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1368 2447 1368
Size: 1079 3456
BsrFI r'CCGG_y
Cuts at: 188 2533 2714 2923 188
Size: 2345 181 209 1800
Fragments arranged by size:
2345 1800 209 181
BssSI C'ACGA_G
Cuts at: 2806 3164 2806
Size: 358 4177
BstAPI GCAn_nnn'nTGC
Cuts at: 1912 1912
Size: 4535
BstBI TT'CG_AA
Cuts at: 578 2896 578
Size: 2318 2217
BstEII G'GTnAC_C
Cuts at: 1524 1524
Size: 4535
BstYI r'GATC_y
Cuts at: 1 641 803 896 1560 2027 2050 2385 2631
Size: 640 162 93 664 467 23 335 246
Cuts at: 2631 3643 3729 3741 4087 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 664 640 467 449 346 335 246
162 93 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1477 1477
Size: 4535
BtgI C'CryG_G
Cuts at: 107 358 938 1457 107
Size: 251 580 519 3185
Fragments arranged by size:
3185 580 519 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2485 2666 2829 2485
Size: 181 163 4191
Fragments arranged by size:
4191 181 163
BtsI GCAGTG_nn'
Cuts at: 192 312 1471 1778 192
Size: 120 1159 307 2949
Fragments arranged by size:
2949 1159 307 120
ClaI AT'CG_AT
Cuts at: 473 473
Size: 4535
DraI TTT'AAA
Cuts at: 1534 1534
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 2241 3099 2241
Size: 858 3677
EaeI y'GGCC_r
Cuts at: 293 335 1341 1389 2120 2294 2685 2712 4057
Size: 42 1006 48 731 174 391 27 1345
Cuts at: 4057 293
Size: 771
Fragments arranged by size:
1345 1006 771 731 391 174 48 42
27
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 1348 1404 2558 2768 1348
Size: 56 1154 210 3115
Fragments arranged by size:
3115 1154 210 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2743 3053 3199 4345 2743
Size: 310 146 1146 2933
Fragments arranged by size:
2933 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1547 1547
Size: 4535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 928 1138 1266 1385 1446 1540 2360 2792
Size: 268 210 128 119 61 94 820 432
Cuts at: 2792 3539 3807 660
Size: 747 268 1388
Fragments arranged by size:
1388 820 747 432 268 268 210 128
119 94 61
EcoNI CCTnn'n_nnAGG
Cuts at: 1015 1245 1015
Size: 230 4305
EcoO109I rG'GnC_Cy
Cuts at: 259 735 923 1405 1481 1674 259
Size: 476 188 482 76 193 3120
Fragments arranged by size:
3120 482 476 193 188 76
EcoRI G'AATT_C
Cuts at: 1539 1539
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1257 2554 1257
Size: 1297 3238
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 509 885 1042 1969 2217 3239 4218 509
Size: 376 157 927 248 1022 979 826
Fragments arranged by size:
1022 979 927 826 376 248 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 307 339 423 455 607 631 639 663 943
Size: 32 84 32 152 24 8 24 280
Cuts at: 943 975 1489 1521 2377 2407 2409 2439 307
Size: 32 514 32 856 30 2 30 2403
Fragments arranged by size:
2403 856 514 280 152 84 32 32
32 32 30 30 24 24 8 2
HincII GTy'rAC
Cuts at: 1008 1574 1008
Size: 566 3969
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MfeI C'AATT_G
Cuts at: 1256 1256
Size: 4535
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 104 549 629 812 3206 3390 104
Size: 445 80 183 2394 184 1249
Fragments arranged by size:
2394 1249 445 184 183 80
MscI TGG'CCA
Cuts at: 295 1391 2296 295
Size: 1096 905 2534
Fragments arranged by size:
2534 1096 905
MslI CAynn'nnrTG
Cuts at: 1456 2524 2651 1456
Size: 1068 127 3340
Fragments arranged by size:
3340 1068 127
MspA1I CmG'CkG
Cuts at: 221 351 660 1200 1646 1960 2320 3333 3578
Size: 130 309 540 446 314 360 1013 245
Cuts at: 3578 221
Size: 1178
Fragments arranged by size:
1178 1013 540 446 360 314 309 245
130
NaeI GCC'GGC
Cuts at: 190 2716 190
Size: 2526 2009
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NcoI C'CATG_G
Cuts at: 107 107
Size: 4535
NgoMIV G'CCGG_C
Cuts at: 188 2714 188
Size: 2526 2009
NspI r_CATG'y
Cuts at: 669 1437 1588 2088 2995 669
Size: 768 151 500 907 2209
Fragments arranged by size:
2209 907 768 500 151
PciI A'CATG_T
Cuts at: 2991 2991
Size: 4535
PflMI CCAn_nnn'nTGG
Cuts at: 777 1111 1424 777
Size: 334 313 3888
Fragments arranged by size:
3888 334 313
PfoI T'CCnGG_A
Cuts at: 905 905
Size: 4535
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1158 1190 3674 3706 1158
Size: 32 2484 32 1987
Fragments arranged by size:
2484 1987 32 32
PpuMI rG'GwC_Cy
Cuts at: 259 735 923 1481 259
Size: 476 188 558 3313
Fragments arranged by size:
3313 558 476 188
PshAI GACnn'nnGTC
Cuts at: 387 844 387
Size: 457 4078
PstI C_TGCA'G
Cuts at: 1582 2267 1582
Size: 685 3850
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 221 660 1200 1960 2320 221
Size: 439 540 760 360 2436
Fragments arranged by size:
2436 760 540 439 360
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 1549 1549
Size: 4535
SalI G'TCGA_C
Cuts at: 1572 1572
Size: 4535
SapI GCTCTTCn'nnn_
Cuts at: 1348 2558 2768 1348
Size: 1210 210 3115
Fragments arranged by size:
3115 1210 210
SbfI CC_TGCA'GG
Cuts at: 1582 1582
Size: 4535
ScaI AGT'ACT
Cuts at: 1396 4052 1396
Size: 2656 1879
SexAI A'CCwGG_T
Cuts at: 1010 1010
Size: 4535
SfcI C'TryA_G
Cuts at: 33 1578 2263 3256 3447 33
Size: 1545 685 993 191 1121
Fragments arranged by size:
1545 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 442 1245 1690 2605 3097 3359 442
Size: 803 445 915 492 262 1618
Fragments arranged by size:
1618 915 803 492 445 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 669 1437 1588 2088 669
Size: 768 151 500 3116
Fragments arranged by size:
3116 768 500 151
StyI C'CwwG_G
Cuts at: 107 1170 1669 2023 107
Size: 1063 499 354 2619
Fragments arranged by size:
2619 1063 499 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2118 2251 2710 2919 2118
Size: 133 459 209 3734
Fragments arranged by size:
3734 459 209 133
TatI w'GTAC_w
Cuts at: 590 1394 4050 590
Size: 804 2656 1075
Fragments arranged by size:
2656 1075 804
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 577 609 1245 1277 4052 4084 577
Size: 32 636 32 2775 32 1028
Fragments arranged by size:
2775 1028 636 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 325 1012 2332 325
Size: 687 1320 2528
Fragments arranged by size:
2528 1320 687
XbaI T'CTAG_A
Cuts at: 151 1566 151
Size: 1415 3120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 657 973 1204 2030 657
Size: 316 231 826 3162
Fragments arranged by size:
3162 826 316 231
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
ZraI GAC'GTC
Cuts at: 454 1238 454
Size: 784 3751
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AlfI AloI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I CspCI DraIII EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI