Restriction map for
pF1KB5815
(Circular) (Six-base) MAPSORT of: pF1KB5815.seq Check: 945 from: 1 to: 4526
pF1KB5815 4526 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 1564 1564
Size: 4526
Acc65I G'GTAC_C
Cuts at: 1542 1542
Size: 4526
AclI AA'CG_TT
Cuts at: 4366 4366
Size: 4526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 2783 3530 3798 2351
Size: 432 747 268 3079
Fragments arranged by size:
3079 747 432 268
AflIII A'CryG_T
Cuts at: 1878 2982 3959 1878
Size: 1104 977 2445
Fragments arranged by size:
2445 1104 977
AgeI A'CCGG_T
Cuts at: 2914 2914
Size: 4526
AleI CACnn'nnGTG
Cuts at: 1408 1408
Size: 4526
AlwNI CAG_nnn'CTG
Cuts at: 463 1440 1831 3398 463
Size: 977 391 1567 1591
Fragments arranged by size:
1591 1567 977 391
ApaI G_GGCC'C
Cuts at: 959 959
Size: 4526
ApaLI G'TGCA_C
Cuts at: 3296 3296
Size: 4526
ApoI r'AATT_y
Cuts at: 693 1530 3879 4135 693
Size: 837 2349 256 1084
Fragments arranged by size:
2349 1084 837 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4526
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
AvaI C'yCGr_G
Cuts at: 272 1546 272
Size: 1274 3252
BamHI G'GATC_C
Cuts at: 1551 1551
Size: 4526
BanI G'GyrC_C
Cuts at: 516 554 1371 1542 1956 2204 2239 516
Size: 38 817 171 414 248 35 2803
Fragments arranged by size:
2803 817 414 248 171 38 35
BanII G_rGCy'C
Cuts at: 959 1001 1192 1540 2570 959
Size: 42 191 348 1030 2915
Fragments arranged by size:
2915 1030 348 191 42
BbeI G_GCGC'C
Cuts at: 558 1960 2208 558
Size: 1402 248 2876
Fragments arranged by size:
2876 1402 248
BciVI GTATCCnnnnn_n'
Cuts at: 2424 3185 4526 2424
Size: 761 1341 2424
Fragments arranged by size:
2424 1341 761
BclI T'GATC_A
Cuts at: 2046 2046
Size: 4526
BglI GCCn_nnn'nGGC
Cuts at: 1445 1445
Size: 4526
BglII A'GATC_T
Cuts at: 1388 2041 4078 1388
Size: 653 2037 1836
Fragments arranged by size:
2037 1836 653
BlpI GC'TnA_GC
Cuts at: 1138 1638 1138
Size: 500 4026
Bme1580I G_kGCm'C
Cuts at: 521 959 1376 2151 2244 3300 521
Size: 438 417 775 93 1056 1747
Fragments arranged by size:
1747 1056 775 438 417 93
BmgBI CAC'GTC
Cuts at: 387 387
Size: 4526
BmrI ACTGGGnnnn_n'
Cuts at: 390 655 1738 1819 2154 4298 390
Size: 265 1083 81 335 2144 618
Fragments arranged by size:
2144 1083 618 335 265 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 732 849 1422 2893 732
Size: 117 573 1471 2365
Fragments arranged by size:
2365 1471 573 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1702 2581 3073 3371 946
Size: 756 879 492 298 2101
Fragments arranged by size:
2101 879 756 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4230 4230
Size: 4526
BsaAI yAC'GTr
Cuts at: 564 1029 1879 564
Size: 465 850 3211
Fragments arranged by size:
3211 850 465
BsaBI GATnn'nnATC
Cuts at: 6 2040 4077 6
Size: 2034 2037 455
Fragments arranged by size:
2037 2034 455
BsaHI Gr'CG_yC
Cuts at: 555 1156 1364 1957 2205 2907 4403 555
Size: 601 208 593 248 702 1496 678
Fragments arranged by size:
1496 702 678 601 593 248 208
BsaWI w'CCGG_w
Cuts at: 1715 2236 2914 3188 3335 3746 1715
Size: 521 678 274 147 411 2495
Fragments arranged by size:
2495 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 607 1270 1288 1297 334
Size: 273 663 18 9 3563
Fragments arranged by size:
3563 663 273 18 9
BseYI C'CCAG_C
Cuts at: 1080 1951 2709 3286 3782 1080
Size: 871 758 577 496 1824
Fragments arranged by size:
1824 871 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1219 1332 694
Size: 525 113 3888
Fragments arranged by size:
3888 525 113
BsiEI CG_ry'CG
Cuts at: 104 243 2068 2114 3322 104
Size: 139 1825 46 1208 1308
Fragments arranged by size:
1825 1308 1208 139 46
BsiHKAI G_wGCw'C
Cuts at: 326 410 1144 1192 1540 2318 2508 3300 326
Size: 84 734 48 348 778 190 792 1552
Fragments arranged by size:
1552 792 778 734 348 190 84 48
BsmI GAATG_Cn'
Cuts at: 1134 1134
Size: 4526
Bsp1286I G_dGCh'C
Cuts at: 326 410 521 959 1001 1144 1192 1376 1540
Size: 84 111 438 42 143 48 184 164
Cuts at: 1540 2151 2244 2318 2508 2570 3300 326
Size: 611 93 74 190 62 730 1552
Fragments arranged by size:
1552 730 611 438 190 184 164 143
111 93 84 74 62 48 42
BspEI T'CCGG_A
Cuts at: 3746 3746
Size: 4526
BspHI T'CATG_A
Cuts at: 1235 3702 1235
Size: 2467 2059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1576 1790 2092 2473 1576
Size: 214 302 381 3629
Fragments arranged by size:
3629 381 302 214
BsrBI CCG'CTC
Cuts at: 50 132 159 200 716 2818 2872 4356 4521
Size: 82 27 41 516 2102 54 1484 165
Cuts at: 4521 50
Size: 55
Fragments arranged by size:
2102 1484 516 165 82 55 54 41
27
BsrDI GCAATG_nn'
Cuts at: 1055 2438 1055
Size: 1383 3143
BsrFI r'CCGG_y
Cuts at: 173 242 513 2524 2705 2914 173
Size: 69 271 2011 181 209 1785
Fragments arranged by size:
2011 1785 271 209 181 69
BssSI C'ACGA_G
Cuts at: 352 2797 3155 352
Size: 2445 358 1723
Fragments arranged by size:
2445 1723 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1903 1903
Size: 4526
BstBI TT'CG_AA
Cuts at: 2887 2887
Size: 4526
BstXI CCAn_nnnn'nTGG
Cuts at: 652 1467 652
Size: 815 3711
BstYI r'GATC_y
Cuts at: 1 782 1343 1388 1551 2018 2041 2376 2622
Size: 781 561 45 163 467 23 335 246
Cuts at: 2622 3634 3720 3732 4078 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 781 561 467 449 346 335 246
163 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 1253 1253
Size: 4526
BtgI C'CryG_G
Cuts at: 521 992 521
Size: 471 4055
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2476 2657 2820 2476
Size: 181 163 4182
Fragments arranged by size:
4182 181 163
BtsI GCAGTG_nn'
Cuts at: 461 1769 461
Size: 1308 3218
DraI TTT'AAA
Cuts at: 1525 1525
Size: 4526
DrdI GACnn_nn'nnGTC
Cuts at: 1039 2232 3090 1039
Size: 1193 858 2475
Fragments arranged by size:
2475 1193 858
EaeI y'GGCC_r
Cuts at: 1209 2111 2285 2676 2703 4048 1209
Size: 902 174 391 27 1345 1687
Fragments arranged by size:
1687 1345 902 391 174 27
EagI C'GGCC_G
Cuts at: 2111 2111
Size: 4526
EarI CTCTTCn'nnn_
Cuts at: 473 2549 2759 473
Size: 2076 210 2240
Fragments arranged by size:
2240 2076 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2734 3044 3190 4336 2734
Size: 310 146 1146 2924
Fragments arranged by size:
2924 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1538 1190
Size: 348 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 2783 3530 3798 2351
Size: 432 747 268 3079
Fragments arranged by size:
3079 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 979 979
Size: 4526
EcoO109I rG'GnC_Cy
Cuts at: 648 956 1224 1437 1665 648
Size: 308 268 213 228 3509
Fragments arranged by size:
3509 308 268 228 213
EcoRI G'AATT_C
Cuts at: 1530 1530
Size: 4526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4526
FspI TGC'GCA
Cuts at: 2307 2307
Size: 4526
HaeII r_GCGC'y
Cuts at: 269 314 558 1960 2208 3230 4209 269
Size: 45 244 1402 248 1022 979 586
Fragments arranged by size:
1402 1022 979 586 248 244 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1457 1480 1489 1512 2368 2398 2400 2430 1457
Size: 23 9 23 856 30 2 30 3553
Fragments arranged by size:
3553 856 30 30 23 23 9 2
HincII GTy'rAC
Cuts at: 1565 1565
Size: 4526
KasI G'GCGC_C
Cuts at: 554 1956 2204 554
Size: 1402 248 2876
Fragments arranged by size:
2876 1402 248
KpnI G_GTAC'C
Cuts at: 1546 1546
Size: 4526
MluI A'CGCG_T
Cuts at: 3959 3959
Size: 4526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 3197 3381 92
Size: 3105 184 1237
Fragments arranged by size:
3105 1237 184
MscI TGG'CCA
Cuts at: 2287 2287
Size: 4526
MslI CAynn'nnrTG
Cuts at: 418 1240 1408 2515 2642 418
Size: 822 168 1107 127 2302
Fragments arranged by size:
2302 1107 822 168 127
MspA1I CmG'CkG
Cuts at: 261 1080 1637 1951 2311 3324 3569 261
Size: 819 557 314 360 1013 245 1218
Fragments arranged by size:
1218 1013 819 557 360 314 245
NaeI GCC'GGC
Cuts at: 2707 2707
Size: 4526
NarI GG'CG_CC
Cuts at: 555 1957 2205 555
Size: 1402 248 2876
Fragments arranged by size:
2876 1402 248
NgoMIV G'CCGG_C
Cuts at: 2705 2705
Size: 4526
NspI r_CATG'y
Cuts at: 822 1077 1579 2079 2986 822
Size: 255 502 500 907 2362
Fragments arranged by size:
2362 907 502 500 255
PciI A'CATG_T
Cuts at: 2982 2982
Size: 4526
PflMI CCAn_nnn'nTGG
Cuts at: 741 741
Size: 4526
PmeI GTTT'AAAC
Cuts at: 1525 1525
Size: 4526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4494
PpuMI rG'GwC_Cy
Cuts at: 1224 1224
Size: 4526
PshAI GACnn'nnGTC
Cuts at: 1222 1222
Size: 4526
PspOMI G'GGCC_C
Cuts at: 955 955
Size: 4526
PstI C_TGCA'G
Cuts at: 1573 2258 1573
Size: 685 3841
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4526
PvuII CAG'CTG
Cuts at: 1080 1951 2311 1080
Size: 871 360 3295
Fragments arranged by size:
3295 871 360
RsrII CG'GwC_CG
Cuts at: 2721 2721
Size: 4526
SacI G_AGCT'C
Cuts at: 1192 1540 1192
Size: 348 4178
SalI G'TCGA_C
Cuts at: 1563 1563
Size: 4526
SapI GCTCTTCn'nnn_
Cuts at: 473 2549 2759 473
Size: 2076 210 2240
Fragments arranged by size:
2240 2076 210
SbfI CC_TGCA'GG
Cuts at: 1573 1573
Size: 4526
ScaI AGT'ACT
Cuts at: 4043 4043
Size: 4526
SexAI A'CCwGG_T
Cuts at: 926 974 926
Size: 48 4478
SfcI C'TryA_G
Cuts at: 33 1569 2254 3247 3438 33
Size: 1536 685 993 191 1121
Fragments arranged by size:
1536 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1445 1445
Size: 4526
SfoI GGC'GCC
Cuts at: 556 1958 2206 556
Size: 1402 248 2876
Fragments arranged by size:
2876 1402 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
SgrAI Cr'CCGG_yG
Cuts at: 513 513
Size: 4526
SmaI CCC'GGG
Cuts at: 274 1548 274
Size: 1274 3252
SmlI C'TyrA_G
Cuts at: 961 1681 2596 3088 3350 961
Size: 720 915 492 262 2137
Fragments arranged by size:
2137 915 720 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4526
SphI G_CATG'C
Cuts at: 1077 1579 2079 1077
Size: 502 500 3524
Fragments arranged by size:
3524 502 500
StuI AGG'CCT
Cuts at: 792 967 792
Size: 175 4351
StyI C'CwwG_G
Cuts at: 1004 1660 2014 1004
Size: 656 354 3516
Fragments arranged by size:
3516 656 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2109 2242 2701 2910 2109
Size: 133 459 209 3725
Fragments arranged by size:
3725 459 209 133
TatI w'GTAC_w
Cuts at: 425 4041 425
Size: 3616 910
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 767 799 4043 4075 767
Size: 32 3244 32 1218
Fragments arranged by size:
3244 1218 32 32
Tth111I GACn'n_nGTC
Cuts at: 928 1363 2323 928
Size: 435 960 3131
Fragments arranged by size:
3131 960 435
XbaI T'CTAG_A
Cuts at: 1557 1557
Size: 4526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 811 2021 811
Size: 1210 3316
XmaI C'CCGG_G
Cuts at: 272 1546 272
Size: 1274 3252
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PsiI PspXI PsrI SacII SanDI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI