Restriction map for
pF1KB5817
(Circular) (Six-base) MAPSORT of: pF1KB5817.seq Check: 3561 from: 1 to: 3749
pF1KB5817 3749 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 787 787
Size: 3749
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 613 1574 2006 2753 3021 232
Size: 381 961 432 747 268 960
Fragments arranged by size:
961 960 747 432 381 268
AflIII A'CryG_T
Cuts at: 521 1101 2205 3182 521
Size: 580 1104 977 1088
Fragments arranged by size:
1104 1088 977 580
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 1054 2621 1054
Size: 1567 2182
ApaLI G'TGCA_C
Cuts at: 2519 2519
Size: 3749
ApoI r'AATT_y
Cuts at: 461 753 3102 3358 461
Size: 292 2349 256 852
Fragments arranged by size:
2349 852 292 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 630 774 630
Size: 144 3605
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 741 763 1793 741
Size: 22 1030 2697
Fragments arranged by size:
2697 1030 22
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BbsI GAAGACnn'nnnn_
Cuts at: 437 437
Size: 3749
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 1374 1467 2523 1374
Size: 93 1056 2600
Fragments arranged by size:
2600 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 961 1042 1377 3521 961
Size: 81 335 2144 1189
Fragments arranged by size:
2144 1189 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 727 742 391
Size: 336 15 3398
Fragments arranged by size:
3398 336 15
Bpu10I CC'TnA_GC
Cuts at: 646 646
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1804 2296 2594 925
Size: 879 492 298 2080
Fragments arranged by size:
2080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3453 3453
Size: 3749
BsaAI yAC'GTr
Cuts at: 524 1102 524
Size: 578 3171
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2411 2558 2969 938
Size: 521 678 274 147 411 1718
Fragments arranged by size:
1718 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 364 394 364
Size: 30 3719
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 508
Size: 3749
BseYI C'CCAG_C
Cuts at: 318 1174 1932 2509 3005 318
Size: 856 758 577 496 1062
Fragments arranged by size:
1062 856 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 652
Size: 3749
BsiEI CG_ry'CG
Cuts at: 104 1291 1337 2545 104
Size: 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 46
BsiHKAI G_wGCw'C
Cuts at: 741 763 1541 1731 2523 741
Size: 22 778 190 792 1967
Fragments arranged by size:
1967 792 778 190 22
Bsp1286I G_dGCh'C
Cuts at: 741 763 1374 1467 1541 1731 1793 2523 741
Size: 22 611 93 74 190 62 730 1967
Fragments arranged by size:
1967 730 611 190 93 74 62 22
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 226 799 1013 1315 1696 226
Size: 573 214 302 381 2279
Fragments arranged by size:
2279 573 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 1747 1928 2137 1747
Size: 181 209 3359
Fragments arranged by size:
3359 209 181
BsrGI T'GTAC_A
Cuts at: 572 572
Size: 3749
BssSI C'ACGA_G
Cuts at: 2020 2378 2020
Size: 358 3391
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstYI r'GATC_y
Cuts at: 1 630 774 1241 1264 1599 1845 2857 2943
Size: 629 144 467 23 335 246 1012 86
Cuts at: 2943 2955 3301 1
Size: 12 346 449
Fragments arranged by size:
1012 629 467 449 346 335 246 144
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 245 992 245
Size: 747 3002
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 605 1334 1508 1899 1926 3271 605
Size: 729 174 391 27 1345 1083
Fragments arranged by size:
1345 1083 729 391 174 27
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 739 761 739
Size: 22 3727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 391 613 727 742 1574 2006 2753 3021
Size: 159 222 114 15 832 432 747 268
Cuts at: 3021 232
Size: 960
Fragments arranged by size:
960 832 747 432 268 222 159 114
15
EcoO109I rG'GnC_Cy
Cuts at: 888 888
Size: 3749
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1768 1768
Size: 3749
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 1183 1431 2453 3432 1183
Size: 248 1022 979 1500
Fragments arranged by size:
1500 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1591 1621 1623 1653 1591
Size: 30 2 30 3687
Fragments arranged by size:
3687 30 30 2
HincII GTy'rAC
Cuts at: 413 788 413
Size: 375 3374
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 607 1510 607
Size: 903 2846
MslI CAynn'nnrTG
Cuts at: 542 1738 1865 542
Size: 1196 127 2426
Fragments arranged by size:
2426 1196 127
MspA1I CmG'CkG
Cuts at: 860 1174 1534 2547 2792 860
Size: 314 360 1013 245 1817
Fragments arranged by size:
1817 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NcoI C'CATG_G
Cuts at: 107 107
Size: 3749
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NspI r_CATG'y
Cuts at: 717 802 1302 2209 717
Size: 85 500 907 2257
Fragments arranged by size:
2257 907 500 85
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PmlI CAC'GTG
Cuts at: 524 524
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PstI C_TGCA'G
Cuts at: 395 796 1481 395
Size: 401 685 2663
Fragments arranged by size:
2663 685 401
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 1174 1534 1174
Size: 360 3389
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 741 763 741
Size: 22 3727
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SfcI C'TryA_G
Cuts at: 33 391 792 1477 2470 2661 33
Size: 358 401 685 993 191 1121
Fragments arranged by size:
1121 993 685 401 358 191
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 904 1819 2311 2573 904
Size: 915 492 262 2080
Fragments arranged by size:
2080 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 717 802 1302 717
Size: 85 500 3164
Fragments arranged by size:
3164 500 85
SspI AAT'ATT
Cuts at: 258 258
Size: 3749
StyI C'CwwG_G
Cuts at: 107 401 455 608 883 1237 107
Size: 294 54 153 275 354 2619
Fragments arranged by size:
2619 354 294 275 153 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 1465 1924 2133 1332
Size: 133 459 209 2948
Fragments arranged by size:
2948 459 209 133
TatI w'GTAC_w
Cuts at: 572 3264 572
Size: 2692 1057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 364 396 615 647 3266 3298 364
Size: 32 219 32 2619 32 815
Fragments arranged by size:
2619 815 219 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1244 1244
Size: 3749
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI