Restriction map for
pF1KB5818
(Circular) (Six-base) MAPSORT of: pF1KB5818.seq Check: 558 from: 1 to: 4541
pF1KB5818 4541 bp
With 182 enzymes: SgfI *
September 25, 2012 15:34 ..
AccI GT'mk_AC
Cuts at: 479 1579 479
Size: 1100 3441
Acc65I G'GTAC_C
Cuts at: 1557 1557
Size: 4541
AclI AA'CG_TT
Cuts at: 4381 4381
Size: 4541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2798 3545 3813 2366
Size: 432 747 268 3094
Fragments arranged by size:
3094 747 432 268
AflIII A'CryG_T
Cuts at: 1214 1893 2997 3974 1214
Size: 679 1104 977 1781
Fragments arranged by size:
1781 1104 977 679
AgeI A'CCGG_T
Cuts at: 2929 2929
Size: 4541
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 302 334 1061 1093 302
Size: 32 727 32 3750
Fragments arranged by size:
3750 727 32 32
AlwNI CAG_nnn'CTG
Cuts at: 385 1846 3413 385
Size: 1461 1567 1513
Fragments arranged by size:
1567 1513 1461
ApaI G_GGCC'C
Cuts at: 1390 1516 1390
Size: 126 4415
ApaLI G'TGCA_C
Cuts at: 3311 3311
Size: 4541
ApoI r'AATT_y
Cuts at: 1545 3894 4150 1545
Size: 2349 256 1936
Fragments arranged by size:
2349 1936 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4541
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
AvaI C'yCGr_G
Cuts at: 210 551 1561 210
Size: 341 1010 3190
Fragments arranged by size:
3190 1010 341
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1268 1301 1268
Size: 33 4508
BamHI G'GATC_C
Cuts at: 1566 1566
Size: 4541
BanI G'GyrC_C
Cuts at: 724 831 1557 1971 2219 2254 724
Size: 107 726 414 248 35 3011
Fragments arranged by size:
3011 726 414 248 107 35
BanII G_rGCy'C
Cuts at: 431 1390 1516 1555 2585 431
Size: 959 126 39 1030 2387
Fragments arranged by size:
2387 1030 959 126 39
BbeI G_GCGC'C
Cuts at: 1975 2223 1975
Size: 248 4293
BbsI GAAGACnn'nnnn_
Cuts at: 1057 1334 1057
Size: 277 4264
BciVI GTATCCnnnnn_n'
Cuts at: 2439 3200 4541 2439
Size: 761 1341 2439
Fragments arranged by size:
2439 1341 761
BclI T'GATC_A
Cuts at: 2061 2061
Size: 4541
BglI GCCn_nnn'nGGC
Cuts at: 369 825 369
Size: 456 4085
BglII A'GATC_T
Cuts at: 2056 4093 2056
Size: 2037 2504
BlpI GC'TnA_GC
Cuts at: 1417 1653 1417
Size: 236 4305
Bme1580I G_kGCm'C
Cuts at: 1390 1516 2166 2259 3315 1390
Size: 126 650 93 1056 2616
Fragments arranged by size:
2616 1056 650 126 93
BmgBI CAC'GTC
Cuts at: 185 185
Size: 4541
BmrI ACTGGGnnnn_n'
Cuts at: 395 1753 1834 2169 4313 395
Size: 1358 81 335 2144 623
Fragments arranged by size:
2144 1358 623 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 4541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1357 857
Size: 500 4041
Bpu10I CC'TnA_GC
Cuts at: 1267 1267
Size: 4541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 1717 2596 3088 3386 806
Size: 911 879 492 298 1961
Fragments arranged by size:
1961 911 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1333 4245 1333
Size: 2912 1629
BsaAI yAC'GTr
Cuts at: 1894 1894
Size: 4541
BsaBI GATnn'nnATC
Cuts at: 6 2055 4092 6
Size: 2049 2037 455
Fragments arranged by size:
2049 2037 455
BsaHI Gr'CG_yC
Cuts at: 1972 2220 2922 4418 1972
Size: 248 702 1496 2095
Fragments arranged by size:
2095 1496 702 248
BsaWI w'CCGG_w
Cuts at: 234 1730 2251 2929 3203 3350 3761 234
Size: 1496 521 678 274 147 411 1014
Fragments arranged by size:
1496 1014 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 830 833 860 863 830
Size: 3 27 3 4508
Fragments arranged by size:
4508 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 586 805 854 253
Size: 333 219 49 3940
Fragments arranged by size:
3940 333 219 49
BseYI C'CCAG_C
Cuts at: 523 720 986 1383 1966 2724 3301 3797 523
Size: 197 266 397 583 758 577 496 1267
Fragments arranged by size:
1267 758 583 577 496 397 266 197
BsiEI CG_ry'CG
Cuts at: 104 660 2083 2129 3337 104
Size: 556 1423 46 1208 1308
Fragments arranged by size:
1423 1308 1208 556 46
BsiHKAI G_wGCw'C
Cuts at: 431 1434 1555 2333 2523 3315 431
Size: 1003 121 778 190 792 1657
Fragments arranged by size:
1657 1003 792 778 190 121
BsmI GAATG_Cn'
Cuts at: 474 474
Size: 4541
BsmBI CGTCTCn'nnnn_
Cuts at: 209 209
Size: 4541
Bsp1286I G_dGCh'C
Cuts at: 431 1390 1434 1516 1555 2166 2259 2333 2523
Size: 959 44 82 39 611 93 74 190
Cuts at: 2523 2585 3315 431
Size: 62 730 1657
Fragments arranged by size:
1657 959 730 611 190 93 82 74
62 44 39
BspEI T'CCGG_A
Cuts at: 234 3761 234
Size: 3527 1014
BspHI T'CATG_A
Cuts at: 3717 3717
Size: 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 980 1199 1591 1805 2107 2488 980
Size: 219 392 214 302 381 3033
Fragments arranged by size:
3033 392 381 302 219 214
BsrBI CCG'CTC
Cuts at: 50 2833 2887 4371 4536 50
Size: 2783 54 1484 165 55
Fragments arranged by size:
2783 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 431 2453 431
Size: 2022 2519
BsrFI r'CCGG_y
Cuts at: 2539 2720 2929 2539
Size: 181 209 4151
Fragments arranged by size:
4151 209 181
BsrGI T'GTAC_A
Cuts at: 1217 1217
Size: 4541
BssSI C'ACGA_G
Cuts at: 2812 3170 2812
Size: 358 4183
BstAPI GCAn_nnn'nTGC
Cuts at: 1179 1918 1179
Size: 739 3802
BstBI TT'CG_AA
Cuts at: 2902 2902
Size: 4541
BstEII G'GTnAC_C
Cuts at: 1363 1363
Size: 4541
BstYI r'GATC_y
Cuts at: 1 1014 1566 2033 2056 2391 2637 3649 3735
Size: 1013 552 467 23 335 246 1012 86
Cuts at: 3735 3747 4093 1
Size: 12 346 449
Fragments arranged by size:
1013 1012 552 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1321 1321
Size: 4541
BtgI C'CryG_G
Cuts at: 107 260 596 920 107
Size: 153 336 324 3728
Fragments arranged by size:
3728 336 324 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 325 2491 2672 2835 325
Size: 2166 181 163 2031
Fragments arranged by size:
2166 2031 181 163
BtsI GCAGTG_nn'
Cuts at: 152 1784 152
Size: 1632 2909
DraI TTT'AAA
Cuts at: 1540 1540
Size: 4541
DrdI GACnn_nn'nnGTC
Cuts at: 2247 3105 2247
Size: 858 3683
EaeI y'GGCC_r
Cuts at: 203 336 614 657 2126 2300 2691 2718 4063
Size: 133 278 43 1469 174 391 27 1345
Cuts at: 4063 203
Size: 681
Fragments arranged by size:
1469 1345 681 391 278 174 133 43
27
EagI C'GGCC_G
Cuts at: 657 2126 657
Size: 1469 3072
EarI CTCTTCn'nnn_
Cuts at: 452 2564 2774 452
Size: 2112 210 2219
Fragments arranged by size:
2219 2112 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 412 2749 3059 3205 4351 412
Size: 2337 310 146 1146 602
Fragments arranged by size:
2337 1146 602 310 146
EcoICRI GAG'CTC
Cuts at: 429 1553 429
Size: 1124 3417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1357 2366 2798 3545 3813 857
Size: 500 1009 432 747 268 1585
Fragments arranged by size:
1585 1009 747 500 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 736 869 736
Size: 133 4408
EcoO109I rG'GnC_Cy
Cuts at: 300 389 1188 1513 1680 300
Size: 89 799 325 167 3161
Fragments arranged by size:
3161 799 325 167 89
EcoRI G'AATT_C
Cuts at: 1545 1545
Size: 4541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 267 1026 2560 267
Size: 759 1534 2248
Fragments arranged by size:
2248 1534 759
FspI TGC'GCA
Cuts at: 2322 2322
Size: 4541
HaeII r_GCGC'y
Cuts at: 1975 2223 3245 4224 1975
Size: 248 1022 979 2292
Fragments arranged by size:
2292 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2383 2413 2415 2445 2383
Size: 30 2 30 4479
Fragments arranged by size:
4479 30 30 2
HincII GTy'rAC
Cuts at: 480 693 1580 480
Size: 213 887 3441
Fragments arranged by size:
3441 887 213
KasI G'GCGC_C
Cuts at: 1971 2219 1971
Size: 248 4293
KpnI G_GTAC'C
Cuts at: 1561 1561
Size: 4541
MluI A'CGCG_T
Cuts at: 3974 3974
Size: 4541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1270 3212 3396 1270
Size: 1942 184 2415
Fragments arranged by size:
2415 1942 184
MscI TGG'CCA
Cuts at: 338 616 2302 338
Size: 278 1686 2577
Fragments arranged by size:
2577 1686 278
MslI CAynn'nnrTG
Cuts at: 1246 1490 2530 2657 1246
Size: 244 1040 127 3130
Fragments arranged by size:
3130 1040 244 127
MspA1I CmG'CkG
Cuts at: 138 527 1652 1966 2326 3339 3584 138
Size: 389 1125 314 360 1013 245 1095
Fragments arranged by size:
1125 1095 1013 389 360 314 245
NaeI GCC'GGC
Cuts at: 2722 2722
Size: 4541
NarI GG'CG_CC
Cuts at: 1972 2220 1972
Size: 248 4293
NcoI C'CATG_G
Cuts at: 107 920 107
Size: 813 3728
NgoMIV G'CCGG_C
Cuts at: 2720 2720
Size: 4541
NspI r_CATG'y
Cuts at: 777 1218 1335 1594 2094 3001 777
Size: 441 117 259 500 907 2317
Fragments arranged by size:
2317 907 500 441 259 117
PasI CC'CwG_GG
Cuts at: 367 367
Size: 4541
PciI A'CATG_T
Cuts at: 1214 2997 1214
Size: 1783 2758
PflMI CCAn_nnn'nTGG
Cuts at: 1396 1509 1396
Size: 113 4428
PfoI T'CCnGG_A
Cuts at: 1310 1310
Size: 4541
PmeI GTTT'AAAC
Cuts at: 1540 1540
Size: 4541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4509
PpuMI rG'GwC_Cy
Cuts at: 1188 1188
Size: 4541
PspOMI G'GGCC_C
Cuts at: 1386 1512 1386
Size: 126 4415
PstI C_TGCA'G
Cuts at: 281 1140 1588 2273 281
Size: 859 448 685 2549
Fragments arranged by size:
2549 859 685 448
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4541
PvuII CAG'CTG
Cuts at: 1966 2326 1966
Size: 360 4181
RsrII CG'GwC_CG
Cuts at: 2736 2736
Size: 4541
SacI G_AGCT'C
Cuts at: 431 1555 431
Size: 1124 3417
SalI G'TCGA_C
Cuts at: 478 1578 478
Size: 1100 3441
SapI GCTCTTCn'nnn_
Cuts at: 2564 2774 2564
Size: 210 4331
SbfI CC_TGCA'GG
Cuts at: 1588 1588
Size: 4541
ScaI AGT'ACT
Cuts at: 967 4058 967
Size: 3091 1450
SfcI C'TryA_G
Cuts at: 33 277 1136 1584 2269 3262 3453 33
Size: 244 859 448 685 993 191 1121
Fragments arranged by size:
1121 993 859 685 448 244 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 369 369
Size: 4541
SfoI GGC'GCC
Cuts at: 1973 2221 1973
Size: 248 4293
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
SmaI CCC'GGG
Cuts at: 553 1563 553
Size: 1010 3531
SmlI C'TyrA_G
Cuts at: 821 1696 2611 3103 3365 821
Size: 875 915 492 262 1997
Fragments arranged by size:
1997 915 875 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4541
SphI G_CATG'C
Cuts at: 777 1594 2094 777
Size: 817 500 3224
Fragments arranged by size:
3224 817 500
SspI AAT'ATT
Cuts at: 1234 1234
Size: 4541
StuI AGG'CCT
Cuts at: 604 748 875 896 1319 604
Size: 144 127 21 423 3826
Fragments arranged by size:
3826 423 144 127 21
StyI C'CwwG_G
Cuts at: 107 305 392 707 920 1010 1352 1675 2029
Size: 198 87 315 213 90 342 323 354
Cuts at: 2029 107
Size: 2619
Fragments arranged by size:
2619 354 342 323 315 213 198 90
87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 712 751 1136 2124 2257 2716 2925 712
Size: 39 385 988 133 459 209 2328
Fragments arranged by size:
2328 988 459 385 209 133 39
TatI w'GTAC_w
Cuts at: 965 1217 1505 4056 965
Size: 252 288 2551 1450
Fragments arranged by size:
2551 1450 288 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 830 833 862 865 4058 4090 830
Size: 3 29 3 3193 32 1281
Fragments arranged by size:
3193 1281 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 2338 2338
Size: 4541
XbaI T'CTAG_A
Cuts at: 1572 1572
Size: 4541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 450 927 2036 450
Size: 477 1109 2955
Fragments arranged by size:
2955 1109 477
XmaI C'CCGG_G
Cuts at: 551 1561 551
Size: 1010 3531
XmnI GAAnn'nnTTC
Cuts at: 247 247
Size: 4541
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BbvCI BcgI BfrBI BmtI BsgI BsiWI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SwaI XhoI ZraI