Restriction map for
pF1KB5826
(Circular) (Six-base) MAPSORT of: pF1KB5826.seq Check: 9456 from: 1 to: 4538
pF1KB5826 4538 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 1576 1576
Size: 4538
Acc65I G'GTAC_C
Cuts at: 1554 1554
Size: 4538
AclI AA'CG_TT
Cuts at: 4378 4378
Size: 4538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 904 961 1291 1428 2363 2795 3542 3810
Size: 806 57 330 137 935 432 747 268
Cuts at: 3810 98
Size: 826
Fragments arranged by size:
935 826 806 747 432 330 268 137
57
AfeI AGC'GCT
Cuts at: 277 277
Size: 4538
AflIII A'CryG_T
Cuts at: 958 1890 2994 3971 958
Size: 932 1104 977 1525
Fragments arranged by size:
1525 1104 977 932
AgeI A'CCGG_T
Cuts at: 2926 2926
Size: 4538
AlwNI CAG_nnn'CTG
Cuts at: 212 257 705 1843 3410 212
Size: 45 448 1138 1567 1340
Fragments arranged by size:
1567 1340 1138 448 45
ApaLI G'TGCA_C
Cuts at: 3308 3308
Size: 4538
ApoI r'AATT_y
Cuts at: 1542 3891 4147 1542
Size: 2349 256 1933
Fragments arranged by size:
2349 1933 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4538
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
AvaI C'yCGr_G
Cuts at: 1558 1558
Size: 4538
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 493 526 493
Size: 33 4505
BamHI G'GATC_C
Cuts at: 981 1519 1563 981
Size: 538 44 3956
Fragments arranged by size:
3956 538 44
BanI G'GyrC_C
Cuts at: 812 1105 1279 1311 1554 1968 2216 2251 812
Size: 293 174 32 243 414 248 35 3099
Fragments arranged by size:
3099 414 293 248 243 174 35 32
BanII G_rGCy'C
Cuts at: 791 1552 2582 791
Size: 761 1030 2747
Fragments arranged by size:
2747 1030 761
BbeI G_GCGC'C
Cuts at: 1972 2220 1972
Size: 248 4290
BbvCI CC'TCA_GC
Cuts at: 952 952
Size: 4538
BciVI GTATCCnnnnn_n'
Cuts at: 2436 3197 4538 2436
Size: 761 1341 2436
Fragments arranged by size:
2436 1341 761
BclI T'GATC_A
Cuts at: 2058 2058
Size: 4538
BfrBI ATG'CAT
Cuts at: 224 224
Size: 4538
BglI GCCn_nnn'nGGC
Cuts at: 760 760
Size: 4538
BglII A'GATC_T
Cuts at: 2053 4090 2053
Size: 2037 2501
BlpI GC'TnA_GC
Cuts at: 822 1650 822
Size: 828 3710
Bme1580I G_kGCm'C
Cuts at: 367 453 1110 1211 2163 2256 3312 367
Size: 86 657 101 952 93 1056 1593
Fragments arranged by size:
1593 1056 952 657 101 93 86
BmrI ACTGGGnnnn_n'
Cuts at: 447 1146 1750 1831 2166 4310 447
Size: 699 604 81 335 2144 675
Fragments arranged by size:
2144 699 675 604 335 81
BmtI G_CTAG'C
Cuts at: 316 316
Size: 4538
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 810 2905 810
Size: 2095 2443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 727 890 508
Size: 219 163 4156
Fragments arranged by size:
4156 219 163
Bpu10I CC'TnA_GC
Cuts at: 952 952
Size: 4538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 511 1714 2593 3085 3383 271
Size: 240 1203 879 492 298 1426
Fragments arranged by size:
1426 1203 879 492 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 1365 4242 1365
Size: 2877 1661
BsaAI yAC'GTr
Cuts at: 1891 1891
Size: 4538
BsaBI GATnn'nnATC
Cuts at: 6 1088 2052 4089 6
Size: 1082 964 2037 455
Fragments arranged by size:
2037 1082 964 455
BsaHI Gr'CG_yC
Cuts at: 1969 2217 2919 4415 1969
Size: 248 702 1496 2092
Fragments arranged by size:
2092 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1727 2248 2926 3200 3347 3758 1727
Size: 521 678 274 147 411 2507
Fragments arranged by size:
2507 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1372 1402 1372
Size: 30 4508
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 508 157
Size: 351 4187
BseYI C'CCAG_C
Cuts at: 440 764 1049 1109 1963 2721 3298 3794 440
Size: 324 285 60 854 758 577 496 1184
Fragments arranged by size:
1184 854 758 577 496 324 285 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 485 676 983 404
Size: 81 191 307 3959
Fragments arranged by size:
3959 307 191 81
BsiEI CG_ry'CG
Cuts at: 104 2080 2126 3334 104
Size: 1976 46 1208 1308
Fragments arranged by size:
1976 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 791 1552 2330 2520 3312 791
Size: 761 778 190 792 2017
Fragments arranged by size:
2017 792 778 761 190
BsmI GAATG_Cn'
Cuts at: 226 226
Size: 4538
Bsp1286I G_dGCh'C
Cuts at: 367 453 791 1110 1211 1552 2163 2256 2330
Size: 86 338 319 101 341 611 93 74
Cuts at: 2330 2520 2582 3312 367
Size: 190 62 730 1593
Fragments arranged by size:
1593 730 611 341 338 319 190 101
93 86 74 62
BspEI T'CCGG_A
Cuts at: 3758 3758
Size: 4538
BspHI T'CATG_A
Cuts at: 3714 3714
Size: 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1588 1802 2104 2485 1588
Size: 214 302 381 3641
Fragments arranged by size:
3641 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2830 2884 4368 4533 50
Size: 2780 54 1484 165 55
Fragments arranged by size:
2780 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2450 2450
Size: 4538
BsrFI r'CCGG_y
Cuts at: 269 2536 2717 2926 269
Size: 2267 181 209 1881
Fragments arranged by size:
2267 1881 209 181
BssSI C'ACGA_G
Cuts at: 2809 3167 2809
Size: 358 4180
BstAPI GCAn_nnn'nTGC
Cuts at: 1215 1915 1215
Size: 700 3838
BstBI TT'CG_AA
Cuts at: 2899 2899
Size: 4538
BstYI r'GATC_y
Cuts at: 1 981 1519 1563 2030 2053 2388 2634 3646
Size: 980 538 44 467 23 335 246 1012
Cuts at: 3646 3732 3744 4090 1
Size: 86 12 346 449
Fragments arranged by size:
1012 980 538 467 449 346 335 246
86 44 23 12
BtgI C'CryG_G
Cuts at: 107 479 1000 1383 107
Size: 372 521 383 3262
Fragments arranged by size:
3262 521 383 372
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2488 2669 2832 2488
Size: 181 163 4194
Fragments arranged by size:
4194 181 163
BtsI GCAGTG_nn'
Cuts at: 392 1781 392
Size: 1389 3149
DraI TTT'AAA
Cuts at: 1537 1537
Size: 4538
DrdI GACnn_nn'nnGTC
Cuts at: 1177 2244 3102 1177
Size: 1067 858 2613
Fragments arranged by size:
2613 1067 858
EaeI y'GGCC_r
Cuts at: 201 2123 2297 2688 2715 4060 201
Size: 1922 174 391 27 1345 679
Fragments arranged by size:
1922 1345 679 391 174 27
EagI C'GGCC_G
Cuts at: 2123 2123
Size: 4538
EarI CTCTTCn'nnn_
Cuts at: 1067 2561 2771 1067
Size: 1494 210 2834
Fragments arranged by size:
2834 1494 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2746 3056 3202 4348 2746
Size: 310 146 1146 2936
Fragments arranged by size:
2936 1146 310 146
EcoICRI GAG'CTC
Cuts at: 789 1550 789
Size: 761 3777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 508 727 890 904 961 1291 1428 2363
Size: 410 219 163 14 57 330 137 935
Cuts at: 2363 2795 3542 3810 98
Size: 432 747 268 826
Fragments arranged by size:
935 826 747 432 410 330 268 219
163 137 57 14
EcoO109I rG'GnC_Cy
Cuts at: 432 777 888 1170 1426 1677 432
Size: 345 111 282 256 251 3293
Fragments arranged by size:
3293 345 282 256 251 111
EcoRI G'AATT_C
Cuts at: 1542 1542
Size: 4538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1054 1227 1251 2557 1054
Size: 173 24 1306 3035
Fragments arranged by size:
3035 1306 173 24
FspI TGC'GCA
Cuts at: 1198 2319 1198
Size: 1121 3417
HaeII r_GCGC'y
Cuts at: 279 1972 2220 3242 4221 279
Size: 1693 248 1022 979 596
Fragments arranged by size:
1693 1022 979 596 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 886 918 1372 1404 2380 2410 2412 2442 886
Size: 32 454 32 976 30 2 30 2982
Fragments arranged by size:
2982 976 454 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1577 1577
Size: 4538
KasI G'GCGC_C
Cuts at: 1968 2216 1968
Size: 248 4290
KpnI G_GTAC'C
Cuts at: 1558 1558
Size: 4538
MluI A'CGCG_T
Cuts at: 3971 3971
Size: 4538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 554 3209 3393 554
Size: 2655 184 1699
Fragments arranged by size:
2655 1699 184
MscI TGG'CCA
Cuts at: 203 2299 203
Size: 2096 2442
MslI CAynn'nnrTG
Cuts at: 2527 2654 2527
Size: 127 4411
MspA1I CmG'CkG
Cuts at: 696 729 1649 1963 2323 3336 3581 696
Size: 33 920 314 360 1013 245 1653
Fragments arranged by size:
1653 1013 920 360 314 245 33
NaeI GCC'GGC
Cuts at: 271 2719 271
Size: 2448 2090
NarI GG'CG_CC
Cuts at: 1969 2217 1969
Size: 248 4290
NcoI C'CATG_G
Cuts at: 107 479 1383 107
Size: 372 904 3262
Fragments arranged by size:
3262 904 372
NgoMIV G'CCGG_C
Cuts at: 269 2717 269
Size: 2448 2090
NheI G'CTAG_C
Cuts at: 312 312
Size: 4538
NsiI A_TGCA'T
Cuts at: 226 226
Size: 4538
NspI r_CATG'y
Cuts at: 155 962 1591 2091 2998 155
Size: 807 629 500 907 1695
Fragments arranged by size:
1695 907 807 629 500
PciI A'CATG_T
Cuts at: 958 2994 958
Size: 2036 2502
PflMI CCAn_nnn'nTGG
Cuts at: 480 1001 480
Size: 521 4017
PfoI T'CCnGG_A
Cuts at: 857 874 1337 857
Size: 17 463 4058
Fragments arranged by size:
4058 463 17
PmeI GTTT'AAAC
Cuts at: 1537 1537
Size: 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4506
PpuMI rG'GwC_Cy
Cuts at: 888 1170 1426 888
Size: 282 256 4000
Fragments arranged by size:
4000 282 256
PstI C_TGCA'G
Cuts at: 701 842 1186 1585 2270 701
Size: 141 344 399 685 2969
Fragments arranged by size:
2969 685 399 344 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4538
PvuII CAG'CTG
Cuts at: 696 729 1963 2323 696
Size: 33 1234 360 2911
Fragments arranged by size:
2911 1234 360 33
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 4538
SacI G_AGCT'C
Cuts at: 791 1552 791
Size: 761 3777
SalI G'TCGA_C
Cuts at: 1575 1575
Size: 4538
SapI GCTCTTCn'nnn_
Cuts at: 2561 2771 2561
Size: 210 4328
SbfI CC_TGCA'GG
Cuts at: 1585 1585
Size: 4538
ScaI AGT'ACT
Cuts at: 4055 4055
Size: 4538
SfcI C'TryA_G
Cuts at: 33 697 781 838 1182 1581 2266 3259 3450
Size: 664 84 57 344 399 685 993 191
Cuts at: 3450 33
Size: 1121
Fragments arranged by size:
1121 993 685 664 399 344 191 84
57
SfiI GGCCn_nnn'nGGCC
Cuts at: 760 760
Size: 4538
SfoI GGC'GCC
Cuts at: 1970 2218 1970
Size: 248 4290
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
SmaI CCC'GGG
Cuts at: 1560 1560
Size: 4538
SmlI C'TyrA_G
Cuts at: 286 490 1693 2608 3100 3362 286
Size: 204 1203 915 492 262 1462
Fragments arranged by size:
1462 1203 915 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4538
SphI G_CATG'C
Cuts at: 1591 2091 1591
Size: 500 4038
SspI AAT'ATT
Cuts at: 1506 1506
Size: 4538
StuI AGG'CCT
Cuts at: 1165 1165
Size: 4538
StyI C'CwwG_G
Cuts at: 107 479 1383 1672 2026 107
Size: 372 904 289 354 2619
Fragments arranged by size:
2619 904 372 354 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1063 1093 1165 2121 2254 2713 2922 1063
Size: 30 72 956 133 459 209 2679
Fragments arranged by size:
2679 956 459 209 133 72 30
TatI w'GTAC_w
Cuts at: 4053 4053
Size: 4538
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 453 485 966 981 998 1013 4055 4087 453
Size: 32 481 15 17 15 3042 32 904
Fragments arranged by size:
3042 904 481 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 2335 2335
Size: 4538
XbaI T'CTAG_A
Cuts at: 1569 1569
Size: 4538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2033 2033
Size: 4538
XmaI C'CCGG_G
Cuts at: 1558 1558
Size: 4538
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbsI BcgI BmgBI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI