(Circular) (Six-base) MAPSORT of: pF1KB5827.seq Check: 3199 from: 1 to: 5180 pF1KB5827 5180 bp With 182 enzymes: SgfI * September 25, 2012 15:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1775 1775 Size: 5180 AccI GT'mk_AC Cuts at: 2218 2218 Size: 5180 Acc65I G'GTAC_C Cuts at: 2196 2196 Size: 5180 AclI AA'CG_TT Cuts at: 5020 5020 Size: 5180 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 3005 3437 4184 4452 3005 Size: 432 747 268 3733 Fragments arranged by size: 3733 747 432 268 AflIII A'CryG_T Cuts at: 2532 3636 4613 2532 Size: 1104 977 3099 Fragments arranged by size: 3099 1104 977 AgeI A'CCGG_T Cuts at: 3568 3568 Size: 5180 AhdI GACnn_n'nnGTC Cuts at: 1611 1611 Size: 5180 AleI CACnn'nnGTG Cuts at: 1777 1777 Size: 5180 AlwNI CAG_nnn'CTG Cuts at: 1313 1379 1455 2485 4052 1313 Size: 66 76 1030 1567 2441 Fragments arranged by size: 2441 1567 1030 76 66 ApaI G_GGCC'C Cuts at: 891 2025 891 Size: 1134 4046 ApaLI G'TGCA_C Cuts at: 3950 3950 Size: 5180 ApoI r'AATT_y Cuts at: 2184 4533 4789 2184 Size: 2349 256 2575 Fragments arranged by size: 2575 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5180 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5180 AvaI C'yCGr_G Cuts at: 220 2200 220 Size: 1980 3200 AvrII C'CTAG_G Cuts at: 819 819 Size: 5180 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1393 1426 1393 Size: 33 5147 BamHI G'GATC_C Cuts at: 2205 2205 Size: 5180 BanI G'GyrC_C Cuts at: 175 181 1994 2196 2610 2858 2893 175 Size: 6 1813 202 414 248 35 2462 Fragments arranged by size: 2462 1813 414 248 202 35 6 BanII G_rGCy'C Cuts at: 692 891 1262 1762 1809 2025 2194 3224 692 Size: 199 371 500 47 216 169 1030 2648 Fragments arranged by size: 2648 1030 500 371 216 199 169 47 BbeI G_GCGC'C Cuts at: 179 185 2614 2862 179 Size: 6 2429 248 2497 Fragments arranged by size: 2497 2429 248 6 BbsI GAAGACnn'nnnn_ Cuts at: 1572 2083 1572 Size: 511 4669 BbvCI CC'TCA_GC Cuts at: 841 1459 841 Size: 618 4562 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 201 235 201 Size: 34 5146 BciVI GTATCCnnnnn_n' Cuts at: 784 3078 3839 5180 784 Size: 2294 761 1341 784 Fragments arranged by size: 2294 1341 784 761 BclI T'GATC_A Cuts at: 2700 2700 Size: 5180 BfrBI ATG'CAT Cuts at: 1020 1020 Size: 5180 BglI GCCn_nnn'nGGC Cuts at: 197 1992 197 Size: 1795 3385 BglII A'GATC_T Cuts at: 2695 4732 2695 Size: 2037 3143 BlpI GC'TnA_GC Cuts at: 592 1372 2292 592 Size: 780 920 3480 Fragments arranged by size: 3480 920 780 Bme1580I G_kGCm'C Cuts at: 891 2025 2154 2805 2898 3954 891 Size: 1134 129 651 93 1056 2117 Fragments arranged by size: 2117 1134 1056 651 129 93 BmrI ACTGGGnnnn_n' Cuts at: 2157 2392 2473 2808 4952 2157 Size: 235 81 335 2144 2385 Fragments arranged by size: 2385 2144 335 235 81 BmtI G_CTAG'C Cuts at: 1682 1682 Size: 5180 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3547 3547 Size: 5180 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 460 706 1335 1381 460 Size: 246 629 46 4259 Fragments arranged by size: 4259 629 246 46 Bpu10I CC'TnA_GC Cuts at: 841 1264 1331 1459 841 Size: 423 67 128 4562 Fragments arranged by size: 4562 423 128 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1420 2026 2356 3235 3727 4025 1420 Size: 606 330 879 492 298 2575 Fragments arranged by size: 2575 879 606 492 330 298 BsaI GGTCTCn'nnnn_ Cuts at: 686 4884 686 Size: 4198 982 BsaAI yAC'GTr Cuts at: 643 2533 643 Size: 1890 3290 BsaBI GATnn'nnATC Cuts at: 6 2694 4731 6 Size: 2688 2037 455 Fragments arranged by size: 2688 2037 455 BsaHI Gr'CG_yC Cuts at: 176 182 2611 2859 3561 5057 176 Size: 6 2429 248 702 1496 299 Fragments arranged by size: 2429 1496 702 299 248 6 BsaWI w'CCGG_w Cuts at: 1610 2369 2890 3568 3842 3989 4400 1610 Size: 759 521 678 274 147 411 2390 Fragments arranged by size: 2390 759 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1117 1147 1117 Size: 30 5150 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 209 389 563 1552 209 Size: 180 174 989 3837 Fragments arranged by size: 3837 989 180 174 BseYI C'CCAG_C Cuts at: 939 960 1114 1734 1786 2168 2605 3363 3940 Size: 21 154 620 52 382 437 758 577 Cuts at: 3940 4436 939 Size: 496 1683 Fragments arranged by size: 1683 758 620 577 496 437 382 154 52 21 BsiEI CG_ry'CG Cuts at: 104 192 2722 2768 3976 104 Size: 88 2530 46 1208 1308 Fragments arranged by size: 2530 1308 1208 88 46 BsiHKAI G_wGCw'C Cuts at: 692 1262 1367 2033 2194 2972 3162 3954 692 Size: 570 105 666 161 778 190 792 1918 Fragments arranged by size: 1918 792 778 666 570 190 161 105 Bsp1286I G_dGCh'C Cuts at: 692 891 1262 1367 1762 1809 2025 2033 2154 Size: 199 371 105 395 47 216 8 121 Cuts at: 2154 2194 2805 2898 2972 3162 3224 3954 692 Size: 40 611 93 74 190 62 730 1918 Fragments arranged by size: 1918 730 611 395 371 216 199 190 121 105 93 74 62 47 40 8 BspEI T'CCGG_A Cuts at: 4400 4400 Size: 5180 BspHI T'CATG_A Cuts at: 308 606 4356 308 Size: 298 3750 1132 Fragments arranged by size: 3750 1132 298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 321 482 892 1518 1683 1775 2163 2230 2444 Size: 161 410 626 165 92 388 67 214 Cuts at: 2444 2746 3127 321 Size: 302 381 2374 Fragments arranged by size: 2374 626 410 388 381 302 214 165 161 92 67 BsrBI CCG'CTC Cuts at: 50 291 1752 3472 3526 5010 5175 50 Size: 241 1461 1720 54 1484 165 55 Fragments arranged by size: 1720 1484 1461 241 165 55 54 BsrDI GCAATG_nn' Cuts at: 3092 3092 Size: 5180 BsrFI r'CCGG_y Cuts at: 178 184 1646 3178 3359 3568 178 Size: 6 1462 1532 181 209 1790 Fragments arranged by size: 1790 1532 1462 209 181 6 BssSI C'ACGA_G Cuts at: 3451 3809 3451 Size: 358 4822 BstAPI GCAn_nnn'nTGC Cuts at: 907 2001 2557 907 Size: 1094 556 3530 Fragments arranged by size: 3530 1094 556 BstBI TT'CG_AA Cuts at: 3541 3541 Size: 5180 BstEII G'GTnAC_C Cuts at: 760 2058 760 Size: 1298 3882 BstXI CCAn_nnnn'nTGG Cuts at: 527 527 Size: 5180 BstYI r'GATC_y Cuts at: 1 1904 2205 2672 2695 3030 3276 4288 4374 Size: 1903 301 467 23 335 246 1012 86 Cuts at: 4374 4386 4732 1 Size: 12 346 449 Fragments arranged by size: 1903 1012 467 449 346 335 301 246 86 23 12 BtgI C'CryG_G Cuts at: 107 809 1775 107 Size: 702 966 3512 Fragments arranged by size: 3512 966 702 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3130 3311 3474 3130 Size: 181 163 4836 Fragments arranged by size: 4836 181 163 BtsI GCAGTG_nn' Cuts at: 1744 2031 2423 1744 Size: 287 392 4501 Fragments arranged by size: 4501 392 287 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 371 406 371 Size: 35 5145 DraI TTT'AAA Cuts at: 2179 2179 Size: 5180 DrdI GACnn_nn'nnGTC Cuts at: 2886 3744 2886 Size: 858 4322 EaeI y'GGCC_r Cuts at: 189 542 1644 2765 2939 3330 3357 4702 189 Size: 353 1102 1121 174 391 27 1345 667 Fragments arranged by size: 1345 1121 1102 667 391 353 174 27 EagI C'GGCC_G Cuts at: 189 2765 189 Size: 2576 2604 EarI CTCTTCn'nnn_ Cuts at: 3203 3413 3203 Size: 210 4970 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3388 3698 3844 4990 3388 Size: 310 146 1146 3578 Fragments arranged by size: 3578 1146 310 146 EcoICRI GAG'CTC Cuts at: 690 1260 2192 690 Size: 570 932 3678 Fragments arranged by size: 3678 932 570 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 460 706 1335 1381 3005 3437 4184 4452 460 Size: 246 629 46 1624 432 747 268 1188 Fragments arranged by size: 1624 1188 747 629 432 268 246 46 EcoNI CCTnn'n_nnAGG Cuts at: 478 919 1033 478 Size: 441 114 4625 Fragments arranged by size: 4625 441 114 EcoO109I rG'GnC_Cy Cuts at: 333 537 845 888 1515 1537 2010 2319 333 Size: 204 308 43 627 22 473 309 3194 Fragments arranged by size: 3194 627 473 309 308 204 43 22 EcoRI G'AATT_C Cuts at: 2184 2184 Size: 5180 EcoRV GAT'ATC Cuts at: 2165 2165 Size: 5180 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1083 3199 1083 Size: 2116 3064 FspI TGC'GCA Cuts at: 2961 2961 Size: 5180 HaeII r_GCGC'y Cuts at: 179 185 220 2614 2862 3884 4863 179 Size: 6 35 2394 248 1022 979 496 Fragments arranged by size: 2394 1022 979 496 248 35 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1597 1629 3022 3052 3054 3084 1597 Size: 32 1393 30 2 30 3693 Fragments arranged by size: 3693 1393 32 30 30 2 HincII GTy'rAC Cuts at: 2219 2219 Size: 5180 HindIII A'AGCT_T Cuts at: 1438 1438 Size: 5180 KasI G'GCGC_C Cuts at: 175 181 2610 2858 175 Size: 6 2429 248 2497 Fragments arranged by size: 2497 2429 248 6 KpnI G_GTAC'C Cuts at: 2200 2200 Size: 5180 MluI A'CGCG_T Cuts at: 4613 4613 Size: 5180 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3851 4035 3851 Size: 184 4996 MscI TGG'CCA Cuts at: 544 2941 544 Size: 2397 2783 MslI CAynn'nnrTG Cuts at: 525 605 880 1777 1814 1973 2036 2131 3169 Size: 80 275 897 37 159 63 95 1038 Cuts at: 3169 3296 525 Size: 127 2409 Fragments arranged by size: 2409 1038 897 275 159 127 95 80 63 37 MspA1I CmG'CkG Cuts at: 1131 1323 1730 1797 2291 2605 2965 3978 4223 Size: 192 407 67 494 314 360 1013 245 Cuts at: 4223 1131 Size: 2088 Fragments arranged by size: 2088 1013 494 407 360 314 245 192 67 NaeI GCC'GGC Cuts at: 180 186 3361 180 Size: 6 3175 1999 Fragments arranged by size: 3175 1999 6 NarI GG'CG_CC Cuts at: 176 182 2611 2859 176 Size: 6 2429 248 2497 Fragments arranged by size: 2497 2429 248 6 NcoI C'CATG_G Cuts at: 107 107 Size: 5180 NdeI CA'TA_TG Cuts at: 526 526 Size: 5180 NgoMIV G'CCGG_C Cuts at: 178 184 3359 178 Size: 6 3175 1999 Fragments arranged by size: 3175 1999 6 NheI G'CTAG_C Cuts at: 1678 1678 Size: 5180 NsiI A_TGCA'T Cuts at: 1022 1022 Size: 5180 NspI r_CATG'y Cuts at: 2048 2233 2733 3640 2048 Size: 185 500 907 3588 Fragments arranged by size: 3588 907 500 185 PasI CC'CwG_GG Cuts at: 891 891 Size: 5180 PciI A'CATG_T Cuts at: 3636 3636 Size: 5180 PflMI CCAn_nnn'nTGG Cuts at: 884 1313 884 Size: 429 4751 PfoI T'CCnGG_A Cuts at: 830 830 Size: 5180 PmeI GTTT'AAAC Cuts at: 2179 2179 Size: 5180 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4319 4351 4319 Size: 32 5148 PpuMI rG'GwC_Cy Cuts at: 333 537 845 1537 333 Size: 204 308 692 3976 Fragments arranged by size: 3976 692 308 204 PshAI GACnn'nnGTC Cuts at: 704 704 Size: 5180 PspOMI G'GGCC_C Cuts at: 887 2021 887 Size: 1134 4046 PstI C_TGCA'G Cuts at: 479 1124 2227 2912 479 Size: 645 1103 685 2747 Fragments arranged by size: 2747 1103 685 645 PvuI CG_AT'CG Cuts at: 104 104 Size: 5180 PvuII CAG'CTG Cuts at: 1131 1323 1730 1797 2605 2965 1131 Size: 192 407 67 808 360 3346 Fragments arranged by size: 3346 808 407 360 192 67 RsrII CG'GwC_CG Cuts at: 3375 3375 Size: 5180 SacI G_AGCT'C Cuts at: 692 1262 2194 692 Size: 570 932 3678 Fragments arranged by size: 3678 932 570 SalI G'TCGA_C Cuts at: 2217 2217 Size: 5180 SapI GCTCTTCn'nnn_ Cuts at: 3203 3413 3203 Size: 210 4970 SbfI CC_TGCA'GG Cuts at: 1124 2227 1124 Size: 1103 4077 ScaI AGT'ACT Cuts at: 4697 4697 Size: 5180 SfcI C'TryA_G Cuts at: 33 475 618 1120 1586 1710 1725 1900 2119 Size: 442 143 502 466 124 15 175 219 Cuts at: 2119 2223 2908 3901 4092 33 Size: 104 685 993 191 1121 Fragments arranged by size: 1121 993 685 502 466 442 219 191 175 143 124 104 15 SfoI GGC'GCC Cuts at: 177 183 2612 2860 177 Size: 6 2429 248 2497 Fragments arranged by size: 2497 2429 248 6 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5180 SgrAI Cr'CCGG_yG Cuts at: 178 184 178 Size: 6 5174 SmaI CCC'GGG Cuts at: 2202 2202 Size: 5180 SmlI C'TyrA_G Cuts at: 1435 2005 2335 3250 3742 4004 1435 Size: 570 330 915 492 262 2611 Fragments arranged by size: 2611 915 570 492 330 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5180 SphI G_CATG'C Cuts at: 2048 2233 2733 2048 Size: 185 500 4495 Fragments arranged by size: 4495 500 185 StuI AGG'CCT Cuts at: 1633 1633 Size: 5180 StyI C'CwwG_G Cuts at: 107 819 1034 1058 2314 2668 107 Size: 712 215 24 1256 354 2619 Fragments arranged by size: 2619 1256 712 354 215 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1128 2763 2896 3355 3564 1128 Size: 1635 133 459 209 2744 Fragments arranged by size: 2744 1635 459 209 133 TatI w'GTAC_w Cuts at: 4695 4695 Size: 5180 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 917 949 1117 1149 4697 4729 917 Size: 32 168 32 3548 32 1368 Fragments arranged by size: 3548 1368 168 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2977 2977 Size: 5180 XbaI T'CTAG_A Cuts at: 2211 2211 Size: 5180 XcmI CCAnnnn_n'nnnnTGG Cuts at: 996 1356 1794 2675 996 Size: 360 438 881 3501 Fragments arranged by size: 3501 881 438 360 XmaI C'CCGG_G Cuts at: 2200 2200 Size: 5180 XmnI GAAnn'nnTTC Cuts at: 1424 1424 Size: 5180 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AlfI AloI AscI BmgBI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII FseI FspAI HpaI MfeI NotI NruI PacI PmlI PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI SwaI XhoI ZraI