Restriction map for
pF1KB5829
(Circular) (Six-base) MAPSORT of: pF1KB5829.seq Check: 8333 from: 1 to: 3485
pF1KB5829 3485 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 523 523
Size: 3485
Acc65I G'GTAC_C
Cuts at: 501 501
Size: 3485
AclI AA'CG_TT
Cuts at: 3325 3325
Size: 3485
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 1310 1742 2489 2757 117
Size: 1193 432 747 268 845
Fragments arranged by size:
1193 845 747 432 268
AflIII A'CryG_T
Cuts at: 837 1941 2918 837
Size: 1104 977 1404
Fragments arranged by size:
1404 1104 977
AgeI A'CCGG_T
Cuts at: 1873 1873
Size: 3485
AlwNI CAG_nnn'CTG
Cuts at: 177 343 790 2357 177
Size: 166 447 1567 1305
Fragments arranged by size:
1567 1305 447 166
ApaLI G'TGCA_C
Cuts at: 2255 2255
Size: 3485
ApoI r'AATT_y
Cuts at: 489 2838 3094 489
Size: 2349 256 880
Fragments arranged by size:
2349 880 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3485
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3485
AvaI C'yCGr_G
Cuts at: 505 505
Size: 3485
BamHI G'GATC_C
Cuts at: 350 510 350
Size: 160 3325
BanI G'GyrC_C
Cuts at: 271 290 501 915 1163 1198 271
Size: 19 211 414 248 35 2558
Fragments arranged by size:
2558 414 248 211 35 19
BanII G_rGCy'C
Cuts at: 228 443 499 1529 228
Size: 215 56 1030 2184
Fragments arranged by size:
2184 1030 215 56
BbeI G_GCGC'C
Cuts at: 919 1167 919
Size: 248 3237
BbvCI CC'TCA_GC
Cuts at: 336 336
Size: 3485
BciVI GTATCCnnnnn_n'
Cuts at: 1383 2144 3485 1383
Size: 761 1341 1383
Fragments arranged by size:
1383 1341 761
BclI T'GATC_A
Cuts at: 450 1005 450
Size: 555 2930
BglII A'GATC_T
Cuts at: 136 1000 3037 136
Size: 864 2037 584
Fragments arranged by size:
2037 864 584
BlpI GC'TnA_GC
Cuts at: 442 597 442
Size: 155 3330
Bme1580I G_kGCm'C
Cuts at: 276 1110 1203 2259 276
Size: 834 93 1056 1502
Fragments arranged by size:
1502 1056 834 93
BmrI ACTGGGnnnn_n'
Cuts at: 433 697 778 1113 3257 433
Size: 264 81 335 2144 661
Fragments arranged by size:
2144 661 335 264 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 416 363
Size: 53 3432
Bpu10I CC'TnA_GC
Cuts at: 336 336
Size: 3485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 661 1540 2032 2330 128
Size: 533 879 492 298 1283
Fragments arranged by size:
1283 879 533 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 182 379 3189 182
Size: 197 2810 478
Fragments arranged by size:
2810 478 197
BsaAI yAC'GTr
Cuts at: 838 838
Size: 3485
BsaBI GATnn'nnATC
Cuts at: 6 999 3036 6
Size: 993 2037 455
Fragments arranged by size:
2037 993 455
BsaHI Gr'CG_yC
Cuts at: 916 1164 1866 3362 916
Size: 248 702 1496 1039
Fragments arranged by size:
1496 1039 702 248
BsaWI w'CCGG_w
Cuts at: 674 1195 1873 2147 2294 2705 674
Size: 521 678 274 147 411 1454
Fragments arranged by size:
1454 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 389 419 389
Size: 30 3455
BseYI C'CCAG_C
Cuts at: 245 910 1668 2245 2741 245
Size: 665 758 577 496 989
Fragments arranged by size:
989 758 665 577 496
BsiEI CG_ry'CG
Cuts at: 104 1027 1073 2281 104
Size: 923 46 1208 1308
Fragments arranged by size:
1308 1208 923 46
BsiHKAI G_wGCw'C
Cuts at: 443 499 1277 1467 2259 443
Size: 56 778 190 792 1669
Fragments arranged by size:
1669 792 778 190 56
Bsp1286I G_dGCh'C
Cuts at: 228 276 443 499 1110 1203 1277 1467 1529
Size: 48 167 56 611 93 74 190 62
Cuts at: 1529 2259 228
Size: 730 1454
Fragments arranged by size:
1454 730 611 190 167 93 74 62
56 48
BspEI T'CCGG_A
Cuts at: 2705 2705
Size: 3485
BspHI T'CATG_A
Cuts at: 2661 2661
Size: 3485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 468 535 749 1051 1432 468
Size: 67 214 302 381 2521
Fragments arranged by size:
2521 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1777 1831 3315 3480 50
Size: 1727 54 1484 165 55
Fragments arranged by size:
1727 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1397 1397
Size: 3485
BsrFI r'CCGG_y
Cuts at: 1483 1664 1873 1483
Size: 181 209 3095
Fragments arranged by size:
3095 209 181
BssSI C'ACGA_G
Cuts at: 1756 2114 1756
Size: 358 3127
BstAPI GCAn_nnn'nTGC
Cuts at: 862 862
Size: 3485
BstBI TT'CG_AA
Cuts at: 1846 1846
Size: 3485
BstYI r'GATC_y
Cuts at: 1 112 136 255 325 350 510 977 1000
Size: 111 24 119 70 25 160 467 23
Cuts at: 1000 1335 1581 2593 2679 2691 3037 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 160 119
111 86 70 25 24 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3485
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1435 1616 1779 1435
Size: 181 163 3141
Fragments arranged by size:
3141 181 163
BtsI GCAGTG_nn'
Cuts at: 162 728 162
Size: 566 2919
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 390 425 390
Size: 35 3450
DraI TTT'AAA
Cuts at: 484 484
Size: 3485
DrdI GACnn_nn'nnGTC
Cuts at: 1191 2049 1191
Size: 858 2627
EaeI y'GGCC_r
Cuts at: 1070 1244 1635 1662 3007 1070
Size: 174 391 27 1345 1548
Fragments arranged by size:
1548 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1070 1070
Size: 3485
EarI CTCTTCn'nnn_
Cuts at: 1508 1718 1508
Size: 210 3275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1693 2003 2149 3295 1693
Size: 310 146 1146 1883
Fragments arranged by size:
1883 1146 310 146
EcoICRI GAG'CTC
Cuts at: 441 497 441
Size: 56 3429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 363 416 1310 1742 2489 2757 117
Size: 246 53 894 432 747 268 845
Fragments arranged by size:
894 845 747 432 268 246 53
EcoO109I rG'GnC_Cy
Cuts at: 174 624 174
Size: 450 3035
EcoRI G'AATT_C
Cuts at: 489 489
Size: 3485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1504 1504
Size: 3485
FspI TGC'GCA
Cuts at: 1266 1266
Size: 3485
HaeII r_GCGC'y
Cuts at: 919 1167 2189 3168 919
Size: 248 1022 979 1236
Fragments arranged by size:
1236 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1327 1357 1359 1389 1327
Size: 30 2 30 3423
Fragments arranged by size:
3423 30 30 2
HincII GTy'rAC
Cuts at: 524 524
Size: 3485
HindIII A'AGCT_T
Cuts at: 146 146
Size: 3485
KasI G'GCGC_C
Cuts at: 915 1163 915
Size: 248 3237
KpnI G_GTAC'C
Cuts at: 505 505
Size: 3485
MluI A'CGCG_T
Cuts at: 2918 2918
Size: 3485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2340 2156
Size: 184 3301
MscI TGG'CCA
Cuts at: 1246 1246
Size: 3485
MslI CAynn'nnrTG
Cuts at: 1474 1601 1474
Size: 127 3358
MspA1I CmG'CkG
Cuts at: 596 910 1270 2283 2528 596
Size: 314 360 1013 245 1553
Fragments arranged by size:
1553 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1666 1666
Size: 3485
NarI GG'CG_CC
Cuts at: 916 1164 916
Size: 248 3237
NcoI C'CATG_G
Cuts at: 107 107
Size: 3485
NgoMIV G'CCGG_C
Cuts at: 1664 1664
Size: 3485
NspI r_CATG'y
Cuts at: 538 1038 1945 538
Size: 500 907 2078
Fragments arranged by size:
2078 907 500
PciI A'CATG_T
Cuts at: 1941 1941
Size: 3485
PfoI T'CCnGG_A
Cuts at: 393 393
Size: 3485
PmeI GTTT'AAAC
Cuts at: 484 484
Size: 3485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2624 2656 2624
Size: 32 3453
PstI C_TGCA'G
Cuts at: 157 532 1217 157
Size: 375 685 2425
Fragments arranged by size:
2425 685 375
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3485
PvuII CAG'CTG
Cuts at: 910 1270 910
Size: 360 3125
RsrII CG'GwC_CG
Cuts at: 1680 1680
Size: 3485
SacI G_AGCT'C
Cuts at: 443 499 443
Size: 56 3429
SalI G'TCGA_C
Cuts at: 522 522
Size: 3485
SapI GCTCTTCn'nnn_
Cuts at: 1508 1718 1508
Size: 210 3275
SbfI CC_TGCA'GG
Cuts at: 532 532
Size: 3485
ScaI AGT'ACT
Cuts at: 3002 3002
Size: 3485
SfcI C'TryA_G
Cuts at: 33 153 528 1213 2206 2397 33
Size: 120 375 685 993 191 1121
Fragments arranged by size:
1121 993 685 375 191 120
SfoI GGC'GCC
Cuts at: 917 1165 917
Size: 248 3237
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3485
SmaI CCC'GGG
Cuts at: 507 507
Size: 3485
SmlI C'TyrA_G
Cuts at: 143 640 1555 2047 2309 143
Size: 497 915 492 262 1319
Fragments arranged by size:
1319 915 497 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3485
SphI G_CATG'C
Cuts at: 538 1038 538
Size: 500 2985
StuI AGG'CCT
Cuts at: 362 384 362
Size: 22 3463
StyI C'CwwG_G
Cuts at: 107 363 619 973 107
Size: 256 256 354 2619
Fragments arranged by size:
2619 354 256 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 259 1068 1201 1660 1869 259
Size: 809 133 459 209 1875
Fragments arranged by size:
1875 809 459 209 133
TatI w'GTAC_w
Cuts at: 3000 3000
Size: 3485
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 389 421 3002 3034 389
Size: 32 2581 32 840
Fragments arranged by size:
2581 840 32 32
Tth111I GACn'n_nGTC
Cuts at: 1282 1282
Size: 3485
XbaI T'CTAG_A
Cuts at: 516 516
Size: 3485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 980 980
Size: 3485
XmaI C'CCGG_G
Cuts at: 505 505
Size: 3485
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI