Restriction map for
pF1KB5830
(Circular) (Six-base) MAPSORT of: pF1KB5830.seq Check: 9576 from: 1 to: 4556
pF1KB5830 4556 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 923 1409 1594 923
Size: 486 185 3885
Fragments arranged by size:
3885 486 185
Acc65I G'GTAC_C
Cuts at: 1572 1572
Size: 4556
AclI AA'CG_TT
Cuts at: 4396 4396
Size: 4556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 772 2381 2813 3560 3828 214
Size: 558 1609 432 747 268 942
Fragments arranged by size:
1609 942 747 558 432 268
AflII C'TTAA_G
Cuts at: 1261 1261
Size: 4556
AflIII A'CryG_T
Cuts at: 1908 3012 3989 1908
Size: 1104 977 2475
Fragments arranged by size:
2475 1104 977
AgeI A'CCGG_T
Cuts at: 2944 2944
Size: 4556
AleI CACnn'nnGTG
Cuts at: 149 149
Size: 4556
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1312 1344 1312
Size: 32 4524
AlwNI CAG_nnn'CTG
Cuts at: 1861 3428 1861
Size: 1567 2989
ApaI G_GGCC'C
Cuts at: 450 864 1298 450
Size: 414 434 3708
Fragments arranged by size:
3708 434 414
ApaLI G'TGCA_C
Cuts at: 3326 3326
Size: 4556
ApoI r'AATT_y
Cuts at: 1432 1560 3909 4165 1432
Size: 128 2349 256 1823
Fragments arranged by size:
2349 1823 256 128
AseI AT'TA_AT
Cuts at: 20 20
Size: 4556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 232 833 1231 1576 232
Size: 601 398 345 3212
Fragments arranged by size:
3212 601 398 345
BamHI G'GATC_C
Cuts at: 1581 1581
Size: 4556
BanI G'GyrC_C
Cuts at: 731 1059 1572 1986 2234 2269 731
Size: 328 513 414 248 35 3018
Fragments arranged by size:
3018 513 414 328 248 35
BanII G_rGCy'C
Cuts at: 450 864 1190 1298 1570 2600 450
Size: 414 326 108 272 1030 2406
Fragments arranged by size:
2406 1030 414 326 272 108
BbeI G_GCGC'C
Cuts at: 1063 1990 2238 1063
Size: 927 248 3381
Fragments arranged by size:
3381 927 248
BbsI GAAGACnn'nnnn_
Cuts at: 304 837 304
Size: 533 4023
BbvCI CC'TCA_GC
Cuts at: 1336 1336
Size: 4556
BciVI GTATCCnnnnn_n'
Cuts at: 2454 3215 4556 2454
Size: 761 1341 2454
Fragments arranged by size:
2454 1341 761
BclI T'GATC_A
Cuts at: 243 2076 243
Size: 1833 2723
BglII A'GATC_T
Cuts at: 2071 4108 2071
Size: 2037 2519
BlpI GC'TnA_GC
Cuts at: 1225 1668 1225
Size: 443 4113
Bme1580I G_kGCm'C
Cuts at: 450 864 1298 2181 2274 3330 450
Size: 414 434 883 93 1056 1676
Fragments arranged by size:
1676 1056 883 434 414 93
BmgBI CAC'GTC
Cuts at: 918 1356 1400 918
Size: 438 44 4074
Fragments arranged by size:
4074 438 44
BmrI ACTGGGnnnn_n'
Cuts at: 482 1437 1515 1768 1849 2184 4328 482
Size: 955 78 253 81 335 2144 710
Fragments arranged by size:
2144 955 710 335 253 81 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 903 2923 903
Size: 2020 2536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 368 703 1093 1513 192
Size: 176 335 390 420 3235
Fragments arranged by size:
3235 420 390 335 176
Bpu10I CC'TnA_GC
Cuts at: 1336 1336
Size: 4556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 916 1732 2611 3103 3401 421
Size: 495 816 879 492 298 1576
Fragments arranged by size:
1576 879 816 495 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4260 4260
Size: 4556
BsaAI yAC'GTr
Cuts at: 375 1909 375
Size: 1534 3022
BsaBI GATnn'nnATC
Cuts at: 6 2070 4107 6
Size: 2064 2037 455
Fragments arranged by size:
2064 2037 455
BsaHI Gr'CG_yC
Cuts at: 1060 1987 2235 2937 4433 1060
Size: 927 248 702 1496 1183
Fragments arranged by size:
1496 1183 927 702 248
BsaWI w'CCGG_w
Cuts at: 1745 2266 2944 3218 3365 3776 1745
Size: 521 678 274 147 411 2525
Fragments arranged by size:
2525 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 256 286 1312 1342 256
Size: 30 1026 30 3470
Fragments arranged by size:
3470 1026 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 412 486 523 868 412
Size: 74 37 345 4100
Fragments arranged by size:
4100 345 74 37
BseYI C'CCAG_C
Cuts at: 1113 1252 1297 1981 2739 3316 3812 1113
Size: 139 45 684 758 577 496 1857
Fragments arranged by size:
1857 758 684 577 496 139 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 896 896
Size: 4556
BsiEI CG_ry'CG
Cuts at: 104 777 1146 2098 2144 3352 104
Size: 673 369 952 46 1208 1308
Fragments arranged by size:
1308 1208 952 673 369 46
BsiHKAI G_wGCw'C
Cuts at: 1190 1325 1570 2348 2538 3330 1190
Size: 135 245 778 190 792 2416
Fragments arranged by size:
2416 792 778 245 190 135
BsmI GAATG_Cn'
Cuts at: 289 289
Size: 4556
Bsp1286I G_dGCh'C
Cuts at: 450 864 1190 1298 1325 1570 2181 2274 2348
Size: 414 326 108 27 245 611 93 74
Cuts at: 2348 2538 2600 3330 450
Size: 190 62 730 1676
Fragments arranged by size:
1676 730 611 414 326 245 190 108
93 74 62 27
BspEI T'CCGG_A
Cuts at: 3776 3776
Size: 4556
BspHI T'CATG_A
Cuts at: 3732 3732
Size: 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1606 1820 2122 2503 1606
Size: 214 302 381 3659
Fragments arranged by size:
3659 381 302 214
BsrBI CCG'CTC
Cuts at: 50 858 2848 2902 4386 4551 50
Size: 808 1990 54 1484 165 55
Fragments arranged by size:
1990 1484 808 165 55 54
BsrDI GCAATG_nn'
Cuts at: 671 2468 671
Size: 1797 2759
BsrFI r'CCGG_y
Cuts at: 2554 2735 2944 2554
Size: 181 209 4166
Fragments arranged by size:
4166 209 181
BssSI C'ACGA_G
Cuts at: 1171 2827 3185 1171
Size: 1656 358 2542
Fragments arranged by size:
2542 1656 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1933 1933
Size: 4556
BstBI TT'CG_AA
Cuts at: 2917 2917
Size: 4556
BstXI CCAn_nnnn'nTGG
Cuts at: 151 420 151
Size: 269 4287
BstYI r'GATC_y
Cuts at: 1 650 1205 1581 2048 2071 2406 2652 3664
Size: 649 555 376 467 23 335 246 1012
Cuts at: 3664 3750 3762 4108 1
Size: 86 12 346 449
Fragments arranged by size:
1012 649 555 467 449 376 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 924 924
Size: 4556
Bsu36I CC'TnA_GG
Cuts at: 1064 1391 1064
Size: 327 4229
BtgI C'CryG_G
Cuts at: 178 464 1097 178
Size: 286 633 3637
Fragments arranged by size:
3637 633 286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1316 2506 2687 2850 1316
Size: 1190 181 163 3022
Fragments arranged by size:
3022 1190 181 163
BtsI GCAGTG_nn'
Cuts at: 349 743 1799 349
Size: 394 1056 3106
Fragments arranged by size:
3106 1056 394
DraI TTT'AAA
Cuts at: 1555 1555
Size: 4556
DrdI GACnn_nn'nnGTC
Cuts at: 2262 3120 2262
Size: 858 3698
EaeI y'GGCC_r
Cuts at: 630 966 1089 1143 1535 2141 2315 2706 2733
Size: 336 123 54 392 606 174 391 27
Cuts at: 2733 4078 630
Size: 1345 1108
Fragments arranged by size:
1345 1108 606 392 391 336 174 123
54 27
EagI C'GGCC_G
Cuts at: 1143 2141 1143
Size: 998 3558
EarI CTCTTCn'nnn_
Cuts at: 190 1186 1196 2579 2789 190
Size: 996 10 1383 210 1957
Fragments arranged by size:
1957 1383 996 210 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2764 3074 3220 4366 2764
Size: 310 146 1146 2954
Fragments arranged by size:
2954 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1188 1568 1188
Size: 380 4176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 214 368 703 772 1093 1513 2381 2813
Size: 22 154 335 69 321 420 868 432
Cuts at: 2813 3560 3828 192
Size: 747 268 920
Fragments arranged by size:
920 868 747 432 420 335 321 268
154 69 22
EcoNI CCTnn'n_nnAGG
Cuts at: 583 658 583
Size: 75 4481
EcoO109I rG'GnC_Cy
Cuts at: 237 365 447 459 714 1043 1269 1294 1695
Size: 128 82 12 255 329 226 25 401
Cuts at: 1695 237
Size: 3098
Fragments arranged by size:
3098 401 329 255 226 128 82 25
12
EcoRI G'AATT_C
Cuts at: 1560 1560
Size: 4556
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 609 777 2575 609
Size: 168 1798 2590
Fragments arranged by size:
2590 1798 168
FspI TGC'GCA
Cuts at: 2337 2337
Size: 4556
HaeII r_GCGC'y
Cuts at: 1063 1179 1990 2238 3260 4239 1063
Size: 116 811 248 1022 979 1380
Fragments arranged by size:
1380 1022 979 811 248 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1225 1257 2398 2428 2430 2460 1225
Size: 32 1141 30 2 30 3321
Fragments arranged by size:
3321 1141 32 30 30 2
HincII GTy'rAC
Cuts at: 1410 1595 1410
Size: 185 4371
KasI G'GCGC_C
Cuts at: 1059 1986 2234 1059
Size: 927 248 3381
Fragments arranged by size:
3381 927 248
KpnI G_GTAC'C
Cuts at: 1576 1576
Size: 4556
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3227 3411 3227
Size: 184 4372
MscI TGG'CCA
Cuts at: 968 2317 968
Size: 1349 3207
MslI CAynn'nnrTG
Cuts at: 149 418 610 1195 2545 2672 149
Size: 269 192 585 1350 127 2033
Fragments arranged by size:
2033 1350 585 269 192 127
MspA1I CmG'CkG
Cuts at: 291 444 1506 1667 1981 2341 3354 3599 291
Size: 153 1062 161 314 360 1013 245 1248
Fragments arranged by size:
1248 1062 1013 360 314 245 161 153
NaeI GCC'GGC
Cuts at: 2737 2737
Size: 4556
NarI GG'CG_CC
Cuts at: 1060 1987 2235 1060
Size: 927 248 3381
Fragments arranged by size:
3381 927 248
NcoI C'CATG_G
Cuts at: 464 464
Size: 4556
NgoMIV G'CCGG_C
Cuts at: 2735 2735
Size: 4556
NotI GC'GGCC_GC
Cuts at: 1143 1143
Size: 4556
NspI r_CATG'y
Cuts at: 1609 2109 3016 1609
Size: 500 907 3149
Fragments arranged by size:
3149 907 500
PciI A'CATG_T
Cuts at: 3012 3012
Size: 4556
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PmlI CAC'GTG
Cuts at: 375 375
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1312 1344 3695 3727 1312
Size: 32 2351 32 2141
Fragments arranged by size:
2351 2141 32 32
PpuMI rG'GwC_Cy
Cuts at: 365 459 714 1043 1269 365
Size: 94 255 329 226 3652
Fragments arranged by size:
3652 329 255 226 94
PshAI GACnn'nnGTC
Cuts at: 352 352
Size: 4556
PspOMI G'GGCC_C
Cuts at: 446 860 1294 446
Size: 414 434 3708
Fragments arranged by size:
3708 434 414
PstI C_TGCA'G
Cuts at: 344 1603 2288 344
Size: 1259 685 2612
Fragments arranged by size:
2612 1259 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 291 1981 2341 291
Size: 1690 360 2506
Fragments arranged by size:
2506 1690 360
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 1190 1570 1190
Size: 380 4176
SalI G'TCGA_C
Cuts at: 1408 1593 1408
Size: 185 4371
SanDI GG'GwC_CC
Cuts at: 1043 1043
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 190 2579 2789 190
Size: 2389 210 1957
Fragments arranged by size:
2389 1957 210
SbfI CC_TGCA'GG
Cuts at: 1603 1603
Size: 4556
ScaI AGT'ACT
Cuts at: 901 4073 901
Size: 3172 1384
SfcI C'TryA_G
Cuts at: 33 340 1599 2284 3277 3468 33
Size: 307 1259 685 993 191 1121
Fragments arranged by size:
1259 1121 993 685 307 191
SfoI GGC'GCC
Cuts at: 1061 1988 2236 1061
Size: 927 248 3381
Fragments arranged by size:
3381 927 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 835 1578 835
Size: 743 3813
SmlI C'TyrA_G
Cuts at: 436 895 1261 1711 2626 3118 3380 436
Size: 459 366 450 915 492 262 1612
Fragments arranged by size:
1612 915 492 459 450 366 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1609 2109 1609
Size: 500 4056
StyI C'CwwG_G
Cuts at: 464 579 677 1307 1690 2044 464
Size: 115 98 630 383 354 2976
Fragments arranged by size:
2976 630 383 354 115 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 1105 2139 2272 2731 2940 316
Size: 789 1034 133 459 209 1932
Fragments arranged by size:
1932 1034 789 459 209 133
TatI w'GTAC_w
Cuts at: 899 4071 899
Size: 3172 1384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 170 202 460 492 4073 4105 170
Size: 32 258 32 3581 32 621
Fragments arranged by size:
3581 621 258 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1357 2353 1357
Size: 996 3560
XbaI T'CTAG_A
Cuts at: 1587 1587
Size: 4556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1314 2051 1314
Size: 737 3819
XmaI C'CCGG_G
Cuts at: 833 1576 833
Size: 743 3813
XmnI GAAnn'nnTTC
Cuts at: 559 1033 559
Size: 474 4082
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsiWI BsmBI BsrGI BssHII
BstEII ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PflMI PfoI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI