Restriction map for
pF1KB5833
(Circular) (Six-base) MAPSORT of: pF1KB5833.seq Check: 3523 from: 1 to: 3638
pF1KB5833 3638 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 676 676
Size: 3638
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 632 3478 632
Size: 2846 792
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AhdI GACnn_n'nnGTC
Cuts at: 456 456
Size: 3638
AlwNI CAG_nnn'CTG
Cuts at: 943 2510 943
Size: 1567 2071
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 642 2991 3247 642
Size: 2349 256 1033
Fragments arranged by size:
2349 1033 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 197 461 560 658 197
Size: 264 99 98 3177
Fragments arranged by size:
3177 264 99 98
BamHI G'GATC_C
Cuts at: 663 663
Size: 3638
BanI G'GyrC_C
Cuts at: 654 1068 1316 1351 654
Size: 414 248 35 2941
Fragments arranged by size:
2941 414 248 35
BanII G_rGCy'C
Cuts at: 652 1682 652
Size: 1030 2608
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BciVI GTATCCnnnnn_n'
Cuts at: 1536 2297 3638 1536
Size: 761 1341 1536
Fragments arranged by size:
1536 1341 761
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglII A'GATC_T
Cuts at: 525 1153 3190 525
Size: 628 2037 973
Fragments arranged by size:
2037 973 628
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 1263 1356 2412 1263
Size: 93 1056 2489
Fragments arranged by size:
2489 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 850 931 1266 3410 850
Size: 81 335 2144 1078
Fragments arranged by size:
2144 1078 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 316 445 550 173
Size: 143 129 105 3261
Fragments arranged by size:
3261 143 129 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1693 2185 2483 814
Size: 879 492 298 1969
Fragments arranged by size:
1969 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 304 3342 304
Size: 3038 600
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 1152 3189 6
Size: 1146 2037 455
Fragments arranged by size:
2037 1146 455
BsaHI Gr'CG_yC
Cuts at: 1069 1317 2019 3515 1069
Size: 248 702 1496 1192
Fragments arranged by size:
1496 1192 702 248
BsaWI w'CCGG_w
Cuts at: 333 827 1348 2026 2300 2447 2858 333
Size: 494 521 678 274 147 411 1113
Fragments arranged by size:
1113 678 521 494 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 283
Size: 30 3608
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 182 412 182
Size: 230 3408
BseYI C'CCAG_C
Cuts at: 1063 1821 2398 2894 1063
Size: 758 577 496 1807
Fragments arranged by size:
1807 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 278 1180 1226 2434 104
Size: 174 902 46 1208 1308
Fragments arranged by size:
1308 1208 902 174 46
BsiHKAI G_wGCw'C
Cuts at: 652 1430 1620 2412 652
Size: 778 190 792 1878
Fragments arranged by size:
1878 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 421 421
Size: 3638
Bsp1286I G_dGCh'C
Cuts at: 652 1263 1356 1430 1620 1682 2412 652
Size: 611 93 74 190 62 730 1878
Fragments arranged by size:
1878 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 333 2858 333
Size: 2525 1113
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 902 1204 1585 688
Size: 214 302 381 2741
Fragments arranged by size:
2741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 327 378 486 1930 1984 3468 3633 50
Size: 277 51 108 1444 54 1484 165 55
Fragments arranged by size:
1484 1444 277 165 108 55 54 51
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 1636 1817 2026 1636
Size: 181 209 3248
Fragments arranged by size:
3248 209 181
BssSI C'ACGA_G
Cuts at: 1909 2267 1909
Size: 358 3280
BstAPI GCAn_nnn'nTGC
Cuts at: 1015 1015
Size: 3638
BstBI TT'CG_AA
Cuts at: 1999 1999
Size: 3638
BstEII G'GTnAC_C
Cuts at: 381 381
Size: 3638
BstYI r'GATC_y
Cuts at: 1 407 525 663 1130 1153 1488 1734 2746
Size: 406 118 138 467 23 335 246 1012
Cuts at: 2746 2832 2844 3190 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 406 346 335 246 138
118 86 23 12
BtgI C'CryG_G
Cuts at: 343 343
Size: 3638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1588 1769 1932 1588
Size: 181 163 3294
Fragments arranged by size:
3294 181 163
BtsI GCAGTG_nn'
Cuts at: 881 881
Size: 3638
DraI TTT'AAA
Cuts at: 637 637
Size: 3638
DrdI GACnn_nn'nnGTC
Cuts at: 435 1344 2202 435
Size: 909 858 1871
Fragments arranged by size:
1871 909 858
EaeI y'GGCC_r
Cuts at: 431 1223 1397 1788 1815 3160 431
Size: 792 174 391 27 1345 909
Fragments arranged by size:
1345 909 792 391 174 27
EagI C'GGCC_G
Cuts at: 1223 1223
Size: 3638
EarI CTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 217 382 1846 2156 2302 3448 217
Size: 165 1464 310 146 1146 407
Fragments arranged by size:
1464 1146 407 310 165 146
EcoICRI GAG'CTC
Cuts at: 650 650
Size: 3638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 316 445 550 1463 1895 2642 2910 173
Size: 143 129 105 913 432 747 268 901
Fragments arranged by size:
913 901 747 432 268 143 129 105
EcoO109I rG'GnC_Cy
Cuts at: 458 777 458
Size: 319 3319
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
EcoRV GAT'ATC
Cuts at: 557 557
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 158 1657 158
Size: 1499 2139
FspI TGC'GCA
Cuts at: 1419 1419
Size: 3638
HaeII r_GCGC'y
Cuts at: 616 1072 1320 2342 3321 616
Size: 456 248 1022 979 933
Fragments arranged by size:
1022 979 933 456 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 405 437 442 474 1480 1510 1512 1542 405
Size: 32 5 32 1006 30 2 30 2501
Fragments arranged by size:
2501 1006 32 32 30 30 5 2
HincII GTy'rAC
Cuts at: 677 677
Size: 3638
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 281 2309 2493 281
Size: 2028 184 1426
Fragments arranged by size:
2028 1426 184
MscI TGG'CCA
Cuts at: 1399 1399
Size: 3638
MslI CAynn'nnrTG
Cuts at: 439 1627 1754 439
Size: 1188 127 2323
Fragments arranged by size:
2323 1188 127
MspA1I CmG'CkG
Cuts at: 359 749 1063 1423 2436 2681 359
Size: 390 314 360 1013 245 1316
Fragments arranged by size:
1316 1013 390 360 314 245
NaeI GCC'GGC
Cuts at: 1819 1819
Size: 3638
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NgoMIV G'CCGG_C
Cuts at: 1817 1817
Size: 3638
NspI r_CATG'y
Cuts at: 691 1191 2098 691
Size: 500 907 2231
Fragments arranged by size:
2231 907 500
PasI CC'CwG_GG
Cuts at: 175 175
Size: 3638
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 3606
PpuMI rG'GwC_Cy
Cuts at: 458 458
Size: 3638
PstI C_TGCA'G
Cuts at: 685 1370 685
Size: 685 2953
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 1063 1423 1063
Size: 360 3278
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 652 652
Size: 3638
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
SbfI CC_TGCA'GG
Cuts at: 685 685
Size: 3638
ScaI AGT'ACT
Cuts at: 3155 3155
Size: 3638
SfcI C'TryA_G
Cuts at: 33 681 1366 2359 2550 33
Size: 648 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 191
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 199 463 660 199
Size: 264 197 3177
Fragments arranged by size:
3177 264 197
SmlI C'TyrA_G
Cuts at: 560 793 1708 2200 2462 560
Size: 233 915 492 262 1736
Fragments arranged by size:
1736 915 492 262 233
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
StyI C'CwwG_G
Cuts at: 772 1126 772
Size: 354 3284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 292 1221 1354 1813 2022 292
Size: 929 133 459 209 1908
Fragments arranged by size:
1908 929 459 209 133
TatI w'GTAC_w
Cuts at: 3153 3153
Size: 3638
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3606
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 145 669 145
Size: 524 3114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1133 1133
Size: 3638
XhoI C'TCGA_G
Cuts at: 560 560
Size: 3638
XmaI C'CCGG_G
Cuts at: 197 461 658 197
Size: 264 197 3177
Fragments arranged by size:
3177 264 197
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI