Restriction map for
pF1KB5835
(Circular) (Six-base) MAPSORT of: pF1KB5835.seq Check: 1076 from: 1 to: 4703
pF1KB5835 4703 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 1741 1741
Size: 4703
Acc65I G'GTAC_C
Cuts at: 1719 1719
Size: 4703
AclI AA'CG_TT
Cuts at: 4543 4543
Size: 4703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1652 2528 2960 3707 3975 718
Size: 934 876 432 747 268 1446
Fragments arranged by size:
1446 934 876 747 432 268
AflII C'TTAA_G
Cuts at: 1375 1375
Size: 4703
AflIII A'CryG_T
Cuts at: 492 2055 3159 4136 492
Size: 1563 1104 977 1059
Fragments arranged by size:
1563 1104 1059 977
AgeI A'CCGG_T
Cuts at: 3091 3091
Size: 4703
AhdI GACnn_n'nnGTC
Cuts at: 672 672
Size: 4703
AlwNI CAG_nnn'CTG
Cuts at: 2008 3575 2008
Size: 1567 3136
ApaI G_GGCC'C
Cuts at: 140 280 1225 140
Size: 140 945 3618
Fragments arranged by size:
3618 945 140
ApaLI G'TGCA_C
Cuts at: 187 3473 187
Size: 3286 1417
ApoI r'AATT_y
Cuts at: 1241 1707 4056 4312 1241
Size: 466 2349 256 1632
Fragments arranged by size:
2349 1632 466 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4703
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
AvaI C'yCGr_G
Cuts at: 1568 1723 1568
Size: 155 4548
BamHI G'GATC_C
Cuts at: 1683 1728 1683
Size: 45 4658
BanI G'GyrC_C
Cuts at: 1719 2133 2381 2416 1719
Size: 414 248 35 4006
Fragments arranged by size:
4006 414 248 35
BanII G_rGCy'C
Cuts at: 140 280 467 1061 1225 1418 1521 1569 1717
Size: 140 187 594 164 193 103 48 148
Cuts at: 1717 2747 140
Size: 1030 2096
Fragments arranged by size:
2096 1030 594 193 187 164 148 140
103 48
BbeI G_GCGC'C
Cuts at: 2137 2385 2137
Size: 248 4455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 893 927 90
Size: 34 769 34 3866
Fragments arranged by size:
3866 769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 681 2601 3362 4703 681
Size: 1920 761 1341 681
Fragments arranged by size:
1920 1341 761 681
BclI T'GATC_A
Cuts at: 1034 1265 2223 1034
Size: 231 958 3514
Fragments arranged by size:
3514 958 231
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4703
BglI GCCn_nnn'nGGC
Cuts at: 1488 1488
Size: 4703
BglII A'GATC_T
Cuts at: 2218 4255 2218
Size: 2037 2666
BlpI GC'TnA_GC
Cuts at: 1815 1815
Size: 4703
Bme1580I G_kGCm'C
Cuts at: 140 191 280 1225 2328 2421 3477 140
Size: 51 89 945 1103 93 1056 1366
Fragments arranged by size:
1366 1103 1056 945 93 89 51
BmrI ACTGGGnnnn_n'
Cuts at: 996 1134 1515 1553 1915 1996 2331 4475 996
Size: 138 381 38 362 81 335 2144 1224
Fragments arranged by size:
2144 1224 381 362 335 138 81 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1535 3070 1535
Size: 1535 3168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 550 1156 1426 1432 451
Size: 99 606 270 6 3722
Fragments arranged by size:
3722 606 270 99 6
Bpu10I CC'TnA_GC
Cuts at: 1462 1462
Size: 4703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 969 1674 1879 2758 3250 3548 969
Size: 705 205 879 492 298 2124
Fragments arranged by size:
2124 879 705 492 298 205
BsaI GGTCTCn'nnnn_
Cuts at: 4407 4407
Size: 4703
BsaAI yAC'GTr
Cuts at: 663 2056 663
Size: 1393 3310
BsaBI GATnn'nnATC
Cuts at: 6 2217 4254 6
Size: 2211 2037 455
Fragments arranged by size:
2211 2037 455
BsaHI Gr'CG_yC
Cuts at: 2134 2382 3084 4580 2134
Size: 248 702 1496 2257
Fragments arranged by size:
2257 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1892 2413 3091 3365 3512 3923 1892
Size: 521 678 274 147 411 2672
Fragments arranged by size:
2672 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 574 604 574
Size: 30 4673
BseYI C'CCAG_C
Cuts at: 280 1330 2128 2886 3463 3959 280
Size: 1050 798 758 577 496 1024
Fragments arranged by size:
1050 1024 798 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 204
Size: 4703
BsiEI CG_ry'CG
Cuts at: 104 2245 2291 3499 104
Size: 2141 46 1208 1308
Fragments arranged by size:
2141 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 191 662 1521 1569 1717 2495 2685 3477 191
Size: 471 859 48 148 778 190 792 1417
Fragments arranged by size:
1417 859 792 778 471 190 148 48
BsmBI CGTCTCn'nnnn_
Cuts at: 680 680
Size: 4703
Bsp1286I G_dGCh'C
Cuts at: 140 191 280 467 662 1061 1225 1418 1521
Size: 51 89 187 195 399 164 193 103
Cuts at: 1521 1569 1717 2328 2421 2495 2685 2747 3477
Size: 48 148 611 93 74 190 62 730
Cuts at: 3477 140
Size: 1366
Fragments arranged by size:
1366 730 611 399 195 193 190 187
164 148 103 93 89 74 62 51
48
BspEI T'CCGG_A
Cuts at: 3923 3923
Size: 4703
BspHI T'CATG_A
Cuts at: 3879 3879
Size: 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 388 1753 1967 2269 2650 388
Size: 1365 214 302 381 2441
Fragments arranged by size:
2441 1365 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2995 3049 4533 4698 50
Size: 2945 54 1484 165 55
Fragments arranged by size:
2945 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 191 702 2615 191
Size: 511 1913 2279
Fragments arranged by size:
2279 1913 511
BsrFI r'CCGG_y
Cuts at: 2701 2882 3091 2701
Size: 181 209 4313
Fragments arranged by size:
4313 209 181
BssSI C'ACGA_G
Cuts at: 2974 3332 2974
Size: 358 4345
BstAPI GCAn_nnn'nTGC
Cuts at: 2080 2080
Size: 4703
BstBI TT'CG_AA
Cuts at: 3064 3064
Size: 4703
BstYI r'GATC_y
Cuts at: 1 1683 1728 2195 2218 2553 2799 3811 3897
Size: 1682 45 467 23 335 246 1012 86
Cuts at: 3897 3909 4255 1
Size: 12 346 449
Fragments arranged by size:
1682 1012 467 449 346 335 246 86
45 23 12
Bsu36I CC'TnA_GG
Cuts at: 1352 1484 1352
Size: 132 4571
BtgI C'CryG_G
Cuts at: 172 172
Size: 4703
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2653 2834 2997 2653
Size: 181 163 4359
Fragments arranged by size:
4359 181 163
BtsI GCAGTG_nn'
Cuts at: 1288 1946 1288
Size: 658 4045
DraI TTT'AAA
Cuts at: 1046 1702 1046
Size: 656 4047
DraIII CAC_nnn'GTG
Cuts at: 433 433
Size: 4703
DrdI GACnn_nn'nnGTC
Cuts at: 2409 3267 2409
Size: 858 3845
EaeI y'GGCC_r
Cuts at: 1307 2288 2462 2853 2880 4225 1307
Size: 981 174 391 27 1345 1785
Fragments arranged by size:
1785 1345 981 391 174 27
EagI C'GGCC_G
Cuts at: 2288 2288
Size: 4703
EarI CTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2911 3221 3367 4513 2911
Size: 310 146 1146 3101
Fragments arranged by size:
3101 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1519 1567 1715 1519
Size: 48 148 4507
Fragments arranged by size:
4507 148 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 550 718 1156 1426 1432 1652 2528 2960
Size: 99 168 438 270 6 220 876 432
Cuts at: 2960 3707 3975 451
Size: 747 268 1179
Fragments arranged by size:
1179 876 747 438 432 270 268 220
168 99 6
EcoNI CCTnn'n_nnAGG
Cuts at: 232 1604 232
Size: 1372 3331
EcoO109I rG'GnC_Cy
Cuts at: 136 137 277 1356 1842 136
Size: 1 140 1079 486 2997
Fragments arranged by size:
2997 1079 486 140 1
EcoRI G'AATT_C
Cuts at: 1241 1707 1241
Size: 466 4237
EcoRV GAT'ATC
Cuts at: 753 753
Size: 4703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4703
FspI TGC'GCA
Cuts at: 2484 2484
Size: 4703
HaeII r_GCGC'y
Cuts at: 2137 2385 3407 4386 2137
Size: 248 1022 979 2454
Fragments arranged by size:
2454 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 658 690 962 994 1515 1547 2545 2575 2577
Size: 32 272 32 521 32 998 30 2
Cuts at: 2577 2607 658
Size: 30 2754
Fragments arranged by size:
2754 998 521 272 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1742 1742
Size: 4703
KasI G'GCGC_C
Cuts at: 2133 2381 2133
Size: 248 4455
KpnI G_GTAC'C
Cuts at: 1723 1723
Size: 4703
MluI A'CGCG_T
Cuts at: 4136 4136
Size: 4703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 560 3374 3558 560
Size: 2814 184 1705
Fragments arranged by size:
2814 1705 184
MscI TGG'CCA
Cuts at: 1309 2464 1309
Size: 1155 3548
MslI CAynn'nnrTG
Cuts at: 794 1157 2692 2819 794
Size: 363 1535 127 2678
Fragments arranged by size:
2678 1535 363 127
MspA1I CmG'CkG
Cuts at: 937 1119 1814 2128 2488 3501 3746 937
Size: 182 695 314 360 1013 245 1894
Fragments arranged by size:
1894 1013 695 360 314 245 182
NaeI GCC'GGC
Cuts at: 2884 2884
Size: 4703
NarI GG'CG_CC
Cuts at: 2134 2382 2134
Size: 248 4455
NdeI CA'TA_TG
Cuts at: 1077 1077
Size: 4703
NgoMIV G'CCGG_C
Cuts at: 2882 2882
Size: 4703
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4703
NspI r_CATG'y
Cuts at: 291 402 496 1395 1438 1756 2256 3163 291
Size: 111 94 899 43 318 500 907 1831
Fragments arranged by size:
1831 907 899 500 318 111 94 43
PciI A'CATG_T
Cuts at: 492 3159 492
Size: 2667 2036
PmeI GTTT'AAAC
Cuts at: 1702 1702
Size: 4703
PmlI CAC'GTG
Cuts at: 663 663
Size: 4703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 238 270 3842 3874 238
Size: 32 3572 32 1067
Fragments arranged by size:
3572 1067 32 32
PpuMI rG'GwC_Cy
Cuts at: 1356 1356
Size: 4703
PshAI GACnn'nnGTC
Cuts at: 1600 1600
Size: 4703
PspOMI G'GGCC_C
Cuts at: 136 276 1221 136
Size: 140 945 3618
Fragments arranged by size:
3618 945 140
PspXI vC'TCGA_Gb
Cuts at: 1568 1568
Size: 4703
PstI C_TGCA'G
Cuts at: 245 268 1750 2435 245
Size: 23 1482 685 2513
Fragments arranged by size:
2513 1482 685 23
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4703
PvuII CAG'CTG
Cuts at: 937 1119 2128 2488 937
Size: 182 1009 360 3152
Fragments arranged by size:
3152 1009 360 182
RsrII CG'GwC_CG
Cuts at: 2898 2898
Size: 4703
SacI G_AGCT'C
Cuts at: 1521 1569 1717 1521
Size: 48 148 4507
Fragments arranged by size:
4507 148 48
SalI G'TCGA_C
Cuts at: 1740 1740
Size: 4703
SapI GCTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
SbfI CC_TGCA'GG
Cuts at: 1750 1750
Size: 4703
ScaI AGT'ACT
Cuts at: 4220 4220
Size: 4703
SfcI C'TryA_G
Cuts at: 33 241 264 740 1746 2431 3424 3615 33
Size: 208 23 476 1006 685 993 191 1121
Fragments arranged by size:
1121 1006 993 685 476 208 191 23
SfoI GGC'GCC
Cuts at: 2135 2383 2135
Size: 248 4455
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
SmaI CCC'GGG
Cuts at: 1725 1725
Size: 4703
SmlI C'TyrA_G
Cuts at: 948 1375 1568 1689 1858 2773 3265 3527 948
Size: 427 193 121 169 915 492 262 2124
Fragments arranged by size:
2124 915 492 427 262 193 169 121
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4703
SphI G_CATG'C
Cuts at: 1395 1756 2256 1395
Size: 361 500 3842
Fragments arranged by size:
3842 500 361
StyI C'CwwG_G
Cuts at: 1205 1837 2191 1205
Size: 632 354 3717
Fragments arranged by size:
3717 632 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1514 2286 2419 2878 3087 1514
Size: 772 133 459 209 3130
Fragments arranged by size:
3130 772 459 209 133
TatI w'GTAC_w
Cuts at: 1451 4218 1451
Size: 2767 1936
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 551 583 4220 4252 551
Size: 32 3637 32 1002
Fragments arranged by size:
3637 1002 32 32
Tth111I GACn'n_nGTC
Cuts at: 2500 2500
Size: 4703
XbaI T'CTAG_A
Cuts at: 1734 1734
Size: 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2198 2198
Size: 4703
XhoI C'TCGA_G
Cuts at: 1568 1568
Size: 4703
XmaI C'CCGG_G
Cuts at: 1723 1723
Size: 4703
XmnI GAAnn'nnTTC
Cuts at: 250 250
Size: 4703
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BmgBI BmtI BseRI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
PacI PasI PflMI PfoI PsiI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI