Restriction map for
pF1KB5836
(Circular) (Six-base) MAPSORT of: pF1KB5836.seq Check: 7026 from: 1 to: 4556
pF1KB5836 4556 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 127 127
Size: 4556
AccI GT'mk_AC
Cuts at: 1594 1594
Size: 4556
Acc65I G'GTAC_C
Cuts at: 1572 1572
Size: 4556
AclI AA'CG_TT
Cuts at: 4396 4396
Size: 4556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 2381 2813 3560 3828 473
Size: 1908 432 747 268 1201
Fragments arranged by size:
1908 1201 747 432 268
AflII C'TTAA_G
Cuts at: 399 399
Size: 4556
AflIII A'CryG_T
Cuts at: 1908 3012 3989 1908
Size: 1104 977 2475
Fragments arranged by size:
2475 1104 977
AgeI A'CCGG_T
Cuts at: 2944 2944
Size: 4556
AleI CACnn'nnGTG
Cuts at: 1124 1124
Size: 4556
AlwNI CAG_nnn'CTG
Cuts at: 1861 3428 1861
Size: 1567 2989
ApaLI G'TGCA_C
Cuts at: 3326 3326
Size: 4556
ApoI r'AATT_y
Cuts at: 507 633 801 1028 1186 1560 3909 4165 507
Size: 126 168 227 158 374 2349 256 898
Fragments arranged by size:
2349 898 374 256 227 168 158 126
AseI AT'TA_AT
Cuts at: 20 389 20
Size: 369 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 1576 1576
Size: 4556
BamHI G'GATC_C
Cuts at: 1581 1581
Size: 4556
BanI G'GyrC_C
Cuts at: 1572 1986 2234 2269 1572
Size: 414 248 35 3859
Fragments arranged by size:
3859 414 248 35
BanII G_rGCy'C
Cuts at: 1167 1570 2600 1167
Size: 403 1030 3123
Fragments arranged by size:
3123 1030 403
BbeI G_GCGC'C
Cuts at: 1990 2238 1990
Size: 248 4308
BbvCI CC'TCA_GC
Cuts at: 152 152
Size: 4556
BciVI GTATCCnnnnn_n'
Cuts at: 1312 2454 3215 4556 1312
Size: 1142 761 1341 1312
Fragments arranged by size:
1341 1312 1142 761
BclI T'GATC_A
Cuts at: 2076 2076
Size: 4556
BglII A'GATC_T
Cuts at: 924 2071 4108 924
Size: 1147 2037 1372
Fragments arranged by size:
2037 1372 1147
BlpI GC'TnA_GC
Cuts at: 1668 1668
Size: 4556
Bme1580I G_kGCm'C
Cuts at: 2181 2274 3330 2181
Size: 93 1056 3407
Fragments arranged by size:
3407 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1768 1849 2184 4328 1768
Size: 81 335 2144 1996
Fragments arranged by size:
2144 1996 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2923 2923
Size: 4556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 263
Size: 4556
Bpu10I CC'TnA_GC
Cuts at: 152 152
Size: 4556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 1732 2611 3103 3401 333
Size: 1399 879 492 298 1488
Fragments arranged by size:
1488 1399 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4260 4260
Size: 4556
BsaAI yAC'GTr
Cuts at: 1909 1909
Size: 4556
BsaBI GATnn'nnATC
Cuts at: 6 2070 4107 6
Size: 2064 2037 455
Fragments arranged by size:
2064 2037 455
BsaHI Gr'CG_yC
Cuts at: 124 1987 2235 2937 4433 124
Size: 1863 248 702 1496 247
Fragments arranged by size:
1863 1496 702 248 247
BsaWI w'CCGG_w
Cuts at: 1745 2266 2944 3218 3365 3776 1745
Size: 521 678 274 147 411 2525
Fragments arranged by size:
2525 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1981 2739 3316 3812 1981
Size: 758 577 496 2725
Fragments arranged by size:
2725 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2098 2144 3352 104
Size: 1994 46 1208 1308
Fragments arranged by size:
1994 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1570 2348 2538 3330 1570
Size: 778 190 792 2796
Fragments arranged by size:
2796 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1167 1570 2181 2274 2348 2538 2600 3330 1167
Size: 403 611 93 74 190 62 730 2393
Fragments arranged by size:
2393 730 611 403 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3776 3776
Size: 4556
BspHI T'CATG_A
Cuts at: 3732 3732
Size: 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1606 1820 2122 2503 1606
Size: 214 302 381 3659
Fragments arranged by size:
3659 381 302 214
BsrBI CCG'CTC
Cuts at: 50 679 2848 2902 4386 4551 50
Size: 629 2169 54 1484 165 55
Fragments arranged by size:
2169 1484 629 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2468 2468
Size: 4556
BsrFI r'CCGG_y
Cuts at: 2554 2735 2944 2554
Size: 181 209 4166
Fragments arranged by size:
4166 209 181
BssSI C'ACGA_G
Cuts at: 2827 3185 2827
Size: 358 4198
BstAPI GCAn_nnn'nTGC
Cuts at: 1933 1933
Size: 4556
BstBI TT'CG_AA
Cuts at: 2917 2917
Size: 4556
BstEII G'GTnAC_C
Cuts at: 759 759
Size: 4556
BstXI CCAn_nnnn'nTGG
Cuts at: 1076 1076
Size: 4556
BstYI r'GATC_y
Cuts at: 1 291 924 1581 2048 2071 2406 2652 3664
Size: 290 633 657 467 23 335 246 1012
Cuts at: 3664 3750 3762 4108 1
Size: 86 12 346 449
Fragments arranged by size:
1012 657 633 467 449 346 335 290
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 248 107
Size: 141 4415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2506 2687 2850 2506
Size: 181 163 4212
Fragments arranged by size:
4212 181 163
BtsI GCAGTG_nn'
Cuts at: 1187 1799 1187
Size: 612 3944
DraI TTT'AAA
Cuts at: 638 1555 638
Size: 917 3639
DrdI GACnn_nn'nnGTC
Cuts at: 2262 3120 2262
Size: 858 3698
EaeI y'GGCC_r
Cuts at: 209 1040 2141 2315 2706 2733 4078 209
Size: 831 1101 174 391 27 1345 687
Fragments arranged by size:
1345 1101 831 687 391 174 27
EagI C'GGCC_G
Cuts at: 2141 2141
Size: 4556
EarI CTCTTCn'nnn_
Cuts at: 449 1295 2579 2789 449
Size: 846 1284 210 2216
Fragments arranged by size:
2216 1284 846 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2764 3074 3220 4366 2764
Size: 310 146 1146 2954
Fragments arranged by size:
2954 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1568 1568
Size: 4556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 473 2381 2813 3560 3828 263
Size: 210 1908 432 747 268 991
Fragments arranged by size:
1908 991 747 432 268 210
EcoNI CCTnn'n_nnAGG
Cuts at: 887 887
Size: 4556
EcoO109I rG'GnC_Cy
Cuts at: 1695 1695
Size: 4556
EcoRI G'AATT_C
Cuts at: 507 1186 1560 507
Size: 679 374 3503
Fragments arranged by size:
3503 679 374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 4556
FspI TGC'GCA
Cuts at: 2337 2337
Size: 4556
HaeII r_GCGC'y
Cuts at: 1343 1990 2238 3260 4239 1343
Size: 647 248 1022 979 1660
Fragments arranged by size:
1660 1022 979 647 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1310 1342 2398 2428 2430 2460 1310
Size: 32 1056 30 2 30 3406
Fragments arranged by size:
3406 1056 32 30 30 2
HincII GTy'rAC
Cuts at: 1508 1595 1508
Size: 87 4469
HpaI GTT'AAC
Cuts at: 1508 1508
Size: 4556
KasI G'GCGC_C
Cuts at: 1986 2234 1986
Size: 248 4308
KpnI G_GTAC'C
Cuts at: 1576 1576
Size: 4556
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 3227 3411 443
Size: 2784 184 1588
Fragments arranged by size:
2784 1588 184
MscI TGG'CCA
Cuts at: 1042 2317 1042
Size: 1275 3281
MslI CAynn'nnrTG
Cuts at: 748 908 936 1124 1310 2545 2672 748
Size: 160 28 188 186 1235 127 2632
Fragments arranged by size:
2632 1235 188 186 160 127 28
MspA1I CmG'CkG
Cuts at: 151 1667 1981 2341 3354 3599 151
Size: 1516 314 360 1013 245 1108
Fragments arranged by size:
1516 1108 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2737 2737
Size: 4556
NarI GG'CG_CC
Cuts at: 1987 2235 1987
Size: 248 4308
NcoI C'CATG_G
Cuts at: 107 248 107
Size: 141 4415
NgoMIV G'CCGG_C
Cuts at: 2735 2735
Size: 4556
NspI r_CATG'y
Cuts at: 1609 2109 3016 1609
Size: 500 907 3149
Fragments arranged by size:
3149 907 500
PacI TTA_AT'TAA
Cuts at: 476 476
Size: 4556
PciI A'CATG_T
Cuts at: 3012 3012
Size: 4556
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4524
PshAI GACnn'nnGTC
Cuts at: 1418 1418
Size: 4556
PstI C_TGCA'G
Cuts at: 199 1603 2288 199
Size: 1404 685 2467
Fragments arranged by size:
2467 1404 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 151 1981 2341 151
Size: 1830 360 2366
Fragments arranged by size:
2366 1830 360
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 1570 1570
Size: 4556
SalI G'TCGA_C
Cuts at: 1593 1593
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 449 1295 2579 2789 449
Size: 846 1284 210 2216
Fragments arranged by size:
2216 1284 846 210
SbfI CC_TGCA'GG
Cuts at: 1603 1603
Size: 4556
ScaI AGT'ACT
Cuts at: 1468 4073 1468
Size: 2605 1951
SfcI C'TryA_G
Cuts at: 33 195 1599 2284 3277 3468 33
Size: 162 1404 685 993 191 1121
Fragments arranged by size:
1404 1121 993 685 191 162
SfoI GGC'GCC
Cuts at: 1988 2236 1988
Size: 248 4308
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 1578 1578
Size: 4556
SmlI C'TyrA_G
Cuts at: 348 399 1711 2626 3118 3380 348
Size: 51 1312 915 492 262 1524
Fragments arranged by size:
1524 1312 915 492 262 51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1609 2109 1609
Size: 500 4056
SspI AAT'ATT
Cuts at: 1156 1156
Size: 4556
StuI AGG'CCT
Cuts at: 1172 1172
Size: 4556
StyI C'CwwG_G
Cuts at: 107 248 1043 1173 1416 1690 2044 107
Size: 141 795 130 243 274 354 2619
Fragments arranged by size:
2619 795 354 274 243 141 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2139 2272 2731 2940 2139
Size: 133 459 209 3755
Fragments arranged by size:
3755 459 209 133
TatI w'GTAC_w
Cuts at: 1466 4071 1466
Size: 2605 1951
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4073 4105 4073
Size: 32 4524
Tth111I GACn'n_nGTC
Cuts at: 2353 2353
Size: 4556
XbaI T'CTAG_A
Cuts at: 1587 1587
Size: 4556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2051 2051
Size: 4556
XmaI C'CCGG_G
Cuts at: 1576 1576
Size: 4556
XmnI GAAnn'nnTTC
Cuts at: 190 734 818 1447 190
Size: 544 84 629 3299
Fragments arranged by size:
3299 629 544 84
ZraI GAC'GTC
Cuts at: 125 125
Size: 4556
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PacI PciI PmeI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PasI PflMI
PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI