Restriction map for
pF1KB5837
(Circular) (Six-base) MAPSORT of: pF1KB5837.seq Check: 2349 from: 1 to: 3833
pF1KB5837 3833 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 502 502
Size: 3833
AccI GT'mk_AC
Cuts at: 871 871
Size: 3833
Acc65I G'GTAC_C
Cuts at: 256 849 256
Size: 593 3240
AclI AA'CG_TT
Cuts at: 3673 3673
Size: 3833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 1658 2090 2837 3105 313
Size: 1345 432 747 268 1041
Fragments arranged by size:
1345 1041 747 432 268
AflIII A'CryG_T
Cuts at: 1185 2289 3266 1185
Size: 1104 977 1752
Fragments arranged by size:
1752 1104 977
AgeI A'CCGG_T
Cuts at: 2221 2221
Size: 3833
AhdI GACnn_n'nnGTC
Cuts at: 414 414
Size: 3833
AleI CACnn'nnGTG
Cuts at: 565 568 565
Size: 3 3830
AlwNI CAG_nnn'CTG
Cuts at: 1138 2705 1138
Size: 1567 2266
ApaI G_GGCC'C
Cuts at: 724 724
Size: 3833
ApaLI G'TGCA_C
Cuts at: 182 2603 182
Size: 2421 1412
ApoI r'AATT_y
Cuts at: 298 837 3186 3442 298
Size: 539 2349 256 689
Fragments arranged by size:
2349 689 539 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
AvaI C'yCGr_G
Cuts at: 229 853 229
Size: 624 3209
BamHI G'GATC_C
Cuts at: 134 858 134
Size: 724 3109
BanI G'GyrC_C
Cuts at: 256 849 1263 1511 1546 256
Size: 593 414 248 35 2543
Fragments arranged by size:
2543 593 414 248 35
BanII G_rGCy'C
Cuts at: 379 724 847 1877 379
Size: 345 123 1030 2335
Fragments arranged by size:
2335 1030 345 123
BbeI G_GCGC'C
Cuts at: 1267 1515 1267
Size: 248 3585
BbvCI CC'TCA_GC
Cuts at: 385 587 653 385
Size: 202 66 3565
Fragments arranged by size:
3565 202 66
BciVI GTATCCnnnnn_n'
Cuts at: 1731 2492 3833 1731
Size: 761 1341 1731
Fragments arranged by size:
1731 1341 761
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3833
BglI GCCn_nnn'nGGC
Cuts at: 817 817
Size: 3833
BglII A'GATC_T
Cuts at: 1348 3385 1348
Size: 2037 1796
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3833
Bme1580I G_kGCm'C
Cuts at: 186 724 1458 1551 2607 186
Size: 538 734 93 1056 1412
Fragments arranged by size:
1412 1056 734 538 93
BmgBI CAC'GTC
Cuts at: 288 521 535 288
Size: 233 14 3586
Fragments arranged by size:
3586 233 14
BmrI ACTGGGnnnn_n'
Cuts at: 1045 1126 1461 3605 1045
Size: 81 335 2144 1273
Fragments arranged by size:
2144 1273 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 3833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 770 770
Size: 3833
Bpu10I CC'TnA_GC
Cuts at: 385 587 653 385
Size: 202 66 3565
Fragments arranged by size:
3565 202 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1888 2380 2678 1009
Size: 879 492 298 2164
Fragments arranged by size:
2164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 733 3537 733
Size: 2804 1029
BsaAI yAC'GTr
Cuts at: 187 1186 187
Size: 999 2834
BsaBI GATnn'nnATC
Cuts at: 6 1347 3384 6
Size: 1341 2037 455
Fragments arranged by size:
2037 1341 455
BsaHI Gr'CG_yC
Cuts at: 1264 1512 2214 3710 1264
Size: 248 702 1496 1387
Fragments arranged by size:
1496 1387 702 248
BsaWI w'CCGG_w
Cuts at: 537 1022 1543 2221 2495 2642 3053 537
Size: 485 521 678 274 147 411 1317
Fragments arranged by size:
1317 678 521 485 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 159 189 159
Size: 30 3803
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 767 767
Size: 3833
BseYI C'CCAG_C
Cuts at: 372 607 705 710 1258 2016 2593 3089 372
Size: 235 98 5 548 758 577 496 1116
Fragments arranged by size:
1116 758 577 548 496 235 98 5
BsiEI CG_ry'CG
Cuts at: 104 1375 1421 2629 104
Size: 1271 46 1208 1308
Fragments arranged by size:
1308 1271 1208 46
BsiHKAI G_wGCw'C
Cuts at: 186 847 1625 1815 2607 186
Size: 661 778 190 792 1412
Fragments arranged by size:
1412 792 778 661 190
Bsp1286I G_dGCh'C
Cuts at: 186 379 724 847 1458 1551 1625 1815 1877
Size: 193 345 123 611 93 74 190 62
Cuts at: 1877 2607 186
Size: 730 1412
Fragments arranged by size:
1412 730 611 345 193 190 123 93
74 62
BspEI T'CCGG_A
Cuts at: 3053 3053
Size: 3833
BspHI T'CATG_A
Cuts at: 3009 3009
Size: 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 502 883 1097 1399 1780 502
Size: 381 214 302 381 2555
Fragments arranged by size:
2555 381 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2125 2179 3663 3828 50
Size: 2075 54 1484 165 55
Fragments arranged by size:
2075 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1745 1745
Size: 3833
BsrFI r'CCGG_y
Cuts at: 117 360 726 1831 2012 2221 117
Size: 243 366 1105 181 209 1729
Fragments arranged by size:
1729 1105 366 243 209 181
BssSI C'ACGA_G
Cuts at: 736 2104 2462 736
Size: 1368 358 2107
Fragments arranged by size:
2107 1368 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1210 1210
Size: 3833
BstBI TT'CG_AA
Cuts at: 2194 2194
Size: 3833
BstXI CCAn_nnnn'nTGG
Cuts at: 567 567
Size: 3833
BstYI r'GATC_y
Cuts at: 1 134 858 1325 1348 1683 1929 2941 3027
Size: 133 724 467 23 335 246 1012 86
Cuts at: 3027 3039 3385 1
Size: 12 346 449
Fragments arranged by size:
1012 724 467 449 346 335 246 133
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 332 332
Size: 3833
BtgI C'CryG_G
Cuts at: 130 210 716 130
Size: 80 506 3247
Fragments arranged by size:
3247 506 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1783 1964 2127 1783
Size: 181 163 3489
Fragments arranged by size:
3489 181 163
BtsI GCAGTG_nn'
Cuts at: 1076 1076
Size: 3833
DraI TTT'AAA
Cuts at: 832 832
Size: 3833
DraIII CAC_nnn'GTG
Cuts at: 190 190
Size: 3833
DrdI GACnn_nn'nnGTC
Cuts at: 1539 2397 1539
Size: 858 2975
EaeI y'GGCC_r
Cuts at: 437 1418 1592 1983 2010 3355 437
Size: 981 174 391 27 1345 915
Fragments arranged by size:
1345 981 915 391 174 27
EagI C'GGCC_G
Cuts at: 1418 1418
Size: 3833
EarI CTCTTCn'nnn_
Cuts at: 229 1856 2066 229
Size: 1627 210 1996
Fragments arranged by size:
1996 1627 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2041 2351 2497 3643 2041
Size: 310 146 1146 2231
Fragments arranged by size:
2231 1146 310 146
EcoICRI GAG'CTC
Cuts at: 845 845
Size: 3833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 770 1658 2090 2837 3105 313
Size: 457 888 432 747 268 1041
Fragments arranged by size:
1041 888 747 457 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 656 656
Size: 3833
EcoO109I rG'GnC_Cy
Cuts at: 972 972
Size: 3833
EcoRI G'AATT_C
Cuts at: 837 837
Size: 3833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3833
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3833
HaeII r_GCGC'y
Cuts at: 385 1267 1515 2537 3516 385
Size: 882 248 1022 979 702
Fragments arranged by size:
1022 979 882 702 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 206 238 400 432 1675 1705 1707 1737 206
Size: 32 162 32 1243 30 2 30 2302
Fragments arranged by size:
2302 1243 162 32 32 30 30 2
HincII GTy'rAC
Cuts at: 872 872
Size: 3833
KasI G'GCGC_C
Cuts at: 1263 1511 1263
Size: 248 3585
KpnI G_GTAC'C
Cuts at: 260 853 260
Size: 593 3240
MluI A'CGCG_T
Cuts at: 3266 3266
Size: 3833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2688 2504
Size: 184 3649
MscI TGG'CCA
Cuts at: 1594 1594
Size: 3833
MslI CAynn'nnrTG
Cuts at: 457 565 568 1822 1949 457
Size: 108 3 1254 127 2341
Fragments arranged by size:
2341 1254 127 108 3
MspA1I CmG'CkG
Cuts at: 132 372 944 1258 1618 2631 2876 132
Size: 240 572 314 360 1013 245 1089
Fragments arranged by size:
1089 1013 572 360 314 245 240
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 3833
NarI GG'CG_CC
Cuts at: 1264 1512 1264
Size: 248 3585
NcoI C'CATG_G
Cuts at: 716 716
Size: 3833
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 3833
NspI r_CATG'y
Cuts at: 577 886 1386 2293 577
Size: 309 500 907 2117
Fragments arranged by size:
2117 907 500 309
PciI A'CATG_T
Cuts at: 2289 2289
Size: 3833
PflMI CCAn_nnn'nTGG
Cuts at: 717 717
Size: 3833
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3833
PmlI CAC'GTG
Cuts at: 187 187
Size: 3833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3801
PspOMI G'GGCC_C
Cuts at: 720 720
Size: 3833
PstI C_TGCA'G
Cuts at: 880 1565 880
Size: 685 3148
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3833
PvuII CAG'CTG
Cuts at: 372 1258 1618 372
Size: 886 360 2587
Fragments arranged by size:
2587 886 360
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3833
SacI G_AGCT'C
Cuts at: 847 847
Size: 3833
SacII CC_GC'GG
Cuts at: 133 133
Size: 3833
SalI G'TCGA_C
Cuts at: 870 870
Size: 3833
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
SbfI CC_TGCA'GG
Cuts at: 880 880
Size: 3833
ScaI AGT'ACT
Cuts at: 3350 3350
Size: 3833
SfcI C'TryA_G
Cuts at: 33 876 1561 2554 2745 33
Size: 843 685 993 191 1121
Fragments arranged by size:
1121 993 843 685 191
SfoI GGC'GCC
Cuts at: 1265 1513 1265
Size: 248 3585
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
SmaI CCC'GGG
Cuts at: 855 855
Size: 3833
SmlI C'TyrA_G
Cuts at: 988 1903 2395 2657 988
Size: 915 492 262 2164
Fragments arranged by size:
2164 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3833
SphI G_CATG'C
Cuts at: 577 886 1386 577
Size: 309 500 3024
Fragments arranged by size:
3024 500 309
StyI C'CwwG_G
Cuts at: 434 716 967 1321 434
Size: 282 251 354 2946
Fragments arranged by size:
2946 354 282 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1416 1549 2008 2217 1416
Size: 133 459 209 3032
Fragments arranged by size:
3032 459 209 133
TatI w'GTAC_w
Cuts at: 3348 3348
Size: 3833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 3801
Tth111I GACn'n_nGTC
Cuts at: 533 1630 533
Size: 1097 2736
XbaI T'CTAG_A
Cuts at: 864 864
Size: 3833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1328 1328
Size: 3833
XmaI C'CCGG_G
Cuts at: 853 853
Size: 3833
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI