Restriction map for
pF1KB5839
(Circular) (Six-base) MAPSORT of: pF1KB5839.seq Check: 1054 from: 1 to: 3722
pF1KB5839 3722 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 760 760
Size: 3722
Acc65I G'GTAC_C
Cuts at: 738 738
Size: 3722
AclI AA'CG_TT
Cuts at: 3562 3562
Size: 3722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 1547 1979 2726 2994 239
Size: 1308 432 747 268 967
Fragments arranged by size:
1308 967 747 432 268
AfeI AGC'GCT
Cuts at: 481 481
Size: 3722
AflIII A'CryG_T
Cuts at: 1074 2178 3155 1074
Size: 1104 977 1641
Fragments arranged by size:
1641 1104 977
AgeI A'CCGG_T
Cuts at: 2110 2110
Size: 3722
AlwNI CAG_nnn'CTG
Cuts at: 1027 2594 1027
Size: 1567 2155
ApaLI G'TGCA_C
Cuts at: 2492 2492
Size: 3722
ApoI r'AATT_y
Cuts at: 726 3075 3331 726
Size: 2349 256 1117
Fragments arranged by size:
2349 1117 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 742 742
Size: 3722
AvrII C'CTAG_G
Cuts at: 327 327
Size: 3722
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 738 1152 1400 1435 738
Size: 414 248 35 3025
Fragments arranged by size:
3025 414 248 35
BanII G_rGCy'C
Cuts at: 736 1766 736
Size: 1030 2692
BbeI G_GCGC'C
Cuts at: 1156 1404 1156
Size: 248 3474
BbsI GAAGACnn'nnnn_
Cuts at: 157 241 685 157
Size: 84 444 3194
Fragments arranged by size:
3194 444 84
BciVI GTATCCnnnnn_n'
Cuts at: 1620 2381 3722 1620
Size: 761 1341 1620
Fragments arranged by size:
1620 1341 761
BclI T'GATC_A
Cuts at: 605 1242 605
Size: 637 3085
BglII A'GATC_T
Cuts at: 1237 3274 1237
Size: 2037 1685
BlpI GC'TnA_GC
Cuts at: 577 834 577
Size: 257 3465
Bme1580I G_kGCm'C
Cuts at: 279 1347 1440 2496 279
Size: 1068 93 1056 1505
Fragments arranged by size:
1505 1068 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 934 1015 1350 3494 934
Size: 81 335 2144 1162
Fragments arranged by size:
2144 1162 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 3722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1777 2269 2567 898
Size: 879 492 298 2053
Fragments arranged by size:
2053 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 308 3426 308
Size: 3118 604
BsaAI yAC'GTr
Cuts at: 1075 1075
Size: 3722
BsaBI GATnn'nnATC
Cuts at: 6 1236 3273 6
Size: 1230 2037 455
Fragments arranged by size:
2037 1230 455
BsaHI Gr'CG_yC
Cuts at: 569 1153 1401 2103 3599 569
Size: 584 248 702 1496 692
Fragments arranged by size:
1496 702 692 584 248
BsaWI w'CCGG_w
Cuts at: 911 1432 2110 2384 2531 2942 911
Size: 521 678 274 147 411 1691
Fragments arranged by size:
1691 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 318 1147 1905 2482 2978 318
Size: 829 758 577 496 1062
Fragments arranged by size:
1062 829 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 582
Size: 3722
BsiEI CG_ry'CG
Cuts at: 104 1264 1310 2518 104
Size: 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 46
BsiHKAI G_wGCw'C
Cuts at: 736 1514 1704 2496 736
Size: 778 190 792 1962
Fragments arranged by size:
1962 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 279 736 1347 1440 1514 1704 1766 2496 279
Size: 457 611 93 74 190 62 730 1505
Fragments arranged by size:
1505 730 611 457 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2942 2942
Size: 3722
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 986 1288 1669 772
Size: 214 302 381 2825
Fragments arranged by size:
2825 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2014 2068 3552 3717 50
Size: 1964 54 1484 165 55
Fragments arranged by size:
1964 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 375 1634 375
Size: 1259 2463
BsrFI r'CCGG_y
Cuts at: 1720 1901 2110 1720
Size: 181 209 3332
Fragments arranged by size:
3332 209 181
BssSI C'ACGA_G
Cuts at: 1993 2351 1993
Size: 358 3364
BstAPI GCAn_nnn'nTGC
Cuts at: 1099 1099
Size: 3722
BstBI TT'CG_AA
Cuts at: 426 2083 426
Size: 1657 2065
BstYI r'GATC_y
Cuts at: 1 747 1214 1237 1572 1818 2830 2916 2928
Size: 746 467 23 335 246 1012 86 12
Cuts at: 2928 3274 1
Size: 346 449
Fragments arranged by size:
1012 746 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 443 443
Size: 3722
BtgI C'CryG_G
Cuts at: 107 287 107
Size: 180 3542
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1672 1853 2016 1672
Size: 181 163 3378
Fragments arranged by size:
3378 181 163
BtsI GCAGTG_nn'
Cuts at: 965 965
Size: 3722
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 1428 2286 1428
Size: 858 2864
EaeI y'GGCC_r
Cuts at: 641 1307 1481 1872 1899 3244 641
Size: 666 174 391 27 1345 1119
Fragments arranged by size:
1345 1119 666 391 174 27
EagI C'GGCC_G
Cuts at: 1307 1307
Size: 3722
EarI CTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 435 1930 2240 2386 3532 435
Size: 1495 310 146 1146 625
Fragments arranged by size:
1495 1146 625 310 146
EcoICRI GAG'CTC
Cuts at: 734 734
Size: 3722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 1547 1979 2726 2994 239
Size: 1308 432 747 268 967
Fragments arranged by size:
1308 967 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 346 861 346
Size: 515 3207
EcoRI G'AATT_C
Cuts at: 726 726
Size: 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FspI TGC'GCA
Cuts at: 391 1503 391
Size: 1112 2610
HaeII r_GCGC'y
Cuts at: 325 483 1156 1404 2426 3405 325
Size: 158 673 248 1022 979 642
Fragments arranged by size:
1022 979 673 642 248 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1564 1594 1596 1626 1564
Size: 30 2 30 3660
Fragments arranged by size:
3660 30 30 2
HincII GTy'rAC
Cuts at: 761 761
Size: 3722
KasI G'GCGC_C
Cuts at: 1152 1400 1152
Size: 248 3474
KpnI G_GTAC'C
Cuts at: 742 742
Size: 3722
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 107 2393 2577 107
Size: 2286 184 1252
Fragments arranged by size:
2286 1252 184
MscI TGG'CCA
Cuts at: 1483 1483
Size: 3722
MslI CAynn'nnrTG
Cuts at: 112 403 1711 1838 112
Size: 291 1308 127 1996
Fragments arranged by size:
1996 1308 291 127
MspA1I CmG'CkG
Cuts at: 833 1147 1507 2520 2765 833
Size: 314 360 1013 245 1790
Fragments arranged by size:
1790 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1903 1903
Size: 3722
NarI GG'CG_CC
Cuts at: 1153 1401 1153
Size: 248 3474
NcoI C'CATG_G
Cuts at: 107 287 107
Size: 180 3542
NgoMIV G'CCGG_C
Cuts at: 1901 1901
Size: 3722
NspI r_CATG'y
Cuts at: 775 1275 2182 775
Size: 500 907 2315
Fragments arranged by size:
2315 907 500
PasI CC'CwG_GG
Cuts at: 279 279
Size: 3722
PciI A'CATG_T
Cuts at: 2178 2178
Size: 3722
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PstI C_TGCA'G
Cuts at: 167 769 1454 167
Size: 602 685 2435
Fragments arranged by size:
2435 685 602
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 1147 1507 1147
Size: 360 3362
RsrII CG'GwC_CG
Cuts at: 1917 1917
Size: 3722
SacI G_AGCT'C
Cuts at: 736 736
Size: 3722
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
SbfI CC_TGCA'GG
Cuts at: 769 769
Size: 3722
ScaI AGT'ACT
Cuts at: 514 3239 514
Size: 2725 997
SfcI C'TryA_G
Cuts at: 33 163 453 655 765 1450 2443 2634 33
Size: 130 290 202 110 685 993 191 1121
Fragments arranged by size:
1121 993 685 290 202 191 130 110
SfoI GGC'GCC
Cuts at: 1154 1402 1154
Size: 248 3474
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 744 744
Size: 3722
SmlI C'TyrA_G
Cuts at: 877 1792 2284 2546 877
Size: 915 492 262 2053
Fragments arranged by size:
2053 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3722
SphI G_CATG'C
Cuts at: 775 1275 775
Size: 500 3222
StyI C'CwwG_G
Cuts at: 107 287 327 856 1210 107
Size: 180 40 529 354 2619
Fragments arranged by size:
2619 529 354 180 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1305 1438 1897 2106 1305
Size: 133 459 209 2921
Fragments arranged by size:
2921 459 209 133
TatI w'GTAC_w
Cuts at: 512 3237 512
Size: 2725 997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 3690
Tth111I GACn'n_nGTC
Cuts at: 1519 1519
Size: 3722
XbaI T'CTAG_A
Cuts at: 540 753 540
Size: 213 3509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 694 1217 694
Size: 523 3199
XmaI C'CCGG_G
Cuts at: 742 742
Size: 3722
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI