Restriction map for
pF1KB5841
(Circular) (Six-base) MAPSORT of: pF1KB5841.seq Check: 9386 from: 1 to: 4322
pF1KB5841 4322 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 984 984
Size: 4322
AccI GT'mk_AC
Cuts at: 1360 1360
Size: 4322
Acc65I G'GTAC_C
Cuts at: 1338 1338
Size: 4322
AclI AA'CG_TT
Cuts at: 4162 4162
Size: 4322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 536 592 1135 2147 2579 3326 3594 351
Size: 185 56 543 1012 432 747 268 1079
Fragments arranged by size:
1079 1012 747 543 432 268 185 56
AfeI AGC'GCT
Cuts at: 610 610
Size: 4322
AflIII A'CryG_T
Cuts at: 1674 2778 3755 1674
Size: 1104 977 2241
Fragments arranged by size:
2241 1104 977
AgeI A'CCGG_T
Cuts at: 2710 2710
Size: 4322
AhdI GACnn_n'nnGTC
Cuts at: 666 666
Size: 4322
AleI CACnn'nnGTG
Cuts at: 561 561
Size: 4322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1231 1231
Size: 4322
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 382 414 751 783 382
Size: 32 337 32 3921
Fragments arranged by size:
3921 337 32 32
AlwNI CAG_nnn'CTG
Cuts at: 940 1627 3194 940
Size: 687 1567 2068
Fragments arranged by size:
2068 1567 687
ApaI G_GGCC'C
Cuts at: 200 739 934 200
Size: 539 195 3588
Fragments arranged by size:
3588 539 195
ApaLI G'TGCA_C
Cuts at: 545 3092 545
Size: 2547 1775
ApoI r'AATT_y
Cuts at: 1326 3675 3931 1326
Size: 2349 256 1717
Fragments arranged by size:
2349 1717 256
AscI GG'CGCG_CC
Cuts at: 538 538
Size: 4322
AseI AT'TA_AT
Cuts at: 20 20
Size: 4322
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
AvaI C'yCGr_G
Cuts at: 616 1342 616
Size: 726 3596
BamHI G'GATC_C
Cuts at: 1347 1347
Size: 4322
BanI G'GyrC_C
Cuts at: 358 497 655 831 915 1338 1752 2000 2035
Size: 139 158 176 84 423 414 248 35
Cuts at: 2035 358
Size: 2645
Fragments arranged by size:
2645 423 414 248 176 158 139 84
35
BanII G_rGCy'C
Cuts at: 200 464 739 934 1184 1336 2366 200
Size: 264 275 195 250 152 1030 2156
Fragments arranged by size:
2156 1030 275 264 250 195 152
BbeI G_GCGC'C
Cuts at: 659 919 1756 2004 659
Size: 260 837 248 2977
Fragments arranged by size:
2977 837 260 248
BciVI GTATCCnnnnn_n'
Cuts at: 2220 2981 4322 2220
Size: 761 1341 2220
Fragments arranged by size:
2220 1341 761
BclI T'GATC_A
Cuts at: 1842 1842
Size: 4322
BglI GCCn_nnn'nGGC
Cuts at: 1258 1258
Size: 4322
BglII A'GATC_T
Cuts at: 1837 3874 1837
Size: 2037 2285
BlpI GC'TnA_GC
Cuts at: 1434 1434
Size: 4322
Bme1580I G_kGCm'C
Cuts at: 200 500 549 739 934 1308 1947 2040 3096
Size: 300 49 190 195 374 639 93 1056
Cuts at: 3096 200
Size: 1426
Fragments arranged by size:
1426 1056 639 374 300 195 190 93
49
BmrI ACTGGGnnnn_n'
Cuts at: 1534 1615 1950 4094 1534
Size: 81 335 2144 1762
Fragments arranged by size:
2144 1762 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 576 2689 576
Size: 2113 2209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 607 625 1312 1330 571
Size: 36 18 687 18 3563
Fragments arranged by size:
3563 687 36 18 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1498 2377 2869 3167 1498
Size: 879 492 298 2653
Fragments arranged by size:
2653 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 247 403 4026 247
Size: 156 3623 543
Fragments arranged by size:
3623 543 156
BsaAI yAC'GTr
Cuts at: 285 349 705 747 876 1038 1675 285
Size: 64 356 42 129 162 637 2932
Fragments arranged by size:
2932 637 356 162 129 64 42
BsaBI GATnn'nnATC
Cuts at: 6 1836 3873 6
Size: 1830 2037 455
Fragments arranged by size:
2037 1830 455
BsaHI Gr'CG_yC
Cuts at: 656 788 916 1753 2001 2703 4199 656
Size: 132 128 837 248 702 1496 779
Fragments arranged by size:
1496 837 779 702 248 132 128
BsaWI w'CCGG_w
Cuts at: 380 1511 2032 2710 2984 3131 3542 380
Size: 1131 521 678 274 147 411 1160
Fragments arranged by size:
1160 1131 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 382 412 584 614 382
Size: 30 172 30 4090
Fragments arranged by size:
4090 172 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 547
Size: 4322
BseYI C'CCAG_C
Cuts at: 643 1059 1232 1747 2505 3082 3578 643
Size: 416 173 515 758 577 496 1387
Fragments arranged by size:
1387 758 577 515 496 416 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 926 933 926
Size: 7 4315
BsiEI CG_ry'CG
Cuts at: 104 476 1864 1910 3118 104
Size: 372 1388 46 1208 1308
Fragments arranged by size:
1388 1308 1208 372 46
BsiHKAI G_wGCw'C
Cuts at: 464 549 1316 1336 2114 2304 3096 464
Size: 85 767 20 778 190 792 1690
Fragments arranged by size:
1690 792 778 767 190 85 20
Bsp1286I G_dGCh'C
Cuts at: 200 464 500 549 739 934 1184 1308 1316
Size: 264 36 49 190 195 250 124 8
Cuts at: 1316 1336 1947 2040 2114 2304 2366 3096 200
Size: 20 611 93 74 190 62 730 1426
Fragments arranged by size:
1426 730 611 264 250 195 190 190
124 93 74 62 49 36 20 8
BspEI T'CCGG_A
Cuts at: 380 3542 380
Size: 3162 1160
BspHI T'CATG_A
Cuts at: 3498 3498
Size: 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 638 984 1372 1586 1888 2269 638
Size: 346 388 214 302 381 2691
Fragments arranged by size:
2691 388 381 346 302 214
BsrBI CCG'CTC
Cuts at: 50 925 2614 2668 4152 4317 50
Size: 875 1689 54 1484 165 55
Fragments arranged by size:
1689 1484 875 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2234 2234
Size: 4322
BsrFI r'CCGG_y
Cuts at: 581 2320 2501 2710 581
Size: 1739 181 209 2193
Fragments arranged by size:
2193 1739 209 181
BssHII G'CGCG_C
Cuts at: 538 538
Size: 4322
BssSI C'ACGA_G
Cuts at: 561 2593 2951 561
Size: 2032 358 1932
Fragments arranged by size:
2032 1932 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1699 1699
Size: 4322
BstBI TT'CG_AA
Cuts at: 2683 2683
Size: 4322
BstEII G'GTnAC_C
Cuts at: 672 672
Size: 4322
BstYI r'GATC_y
Cuts at: 1 1347 1814 1837 2172 2418 3430 3516 3528
Size: 1346 467 23 335 246 1012 86 12
Cuts at: 3528 3874 1
Size: 346 449
Fragments arranged by size:
1346 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 121 146 121
Size: 25 4297
BtgI C'CryG_G
Cuts at: 107 791 107
Size: 684 3638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2272 2453 2616 2272
Size: 181 163 3978
Fragments arranged by size:
3978 181 163
BtsI GCAGTG_nn'
Cuts at: 1003 1565 1003
Size: 562 3760
ClaI AT'CG_AT
Cuts at: 752 752
Size: 4322
DraI TTT'AAA
Cuts at: 1321 1321
Size: 4322
DraIII CAC_nnn'GTG
Cuts at: 133 133
Size: 4322
DrdI GACnn_nn'nnGTC
Cuts at: 793 2028 2886 793
Size: 1235 858 2229
Fragments arranged by size:
2229 1235 858
EaeI y'GGCC_r
Cuts at: 524 1102 1907 2081 2472 2499 3844 524
Size: 578 805 174 391 27 1345 1002
Fragments arranged by size:
1345 1002 805 578 391 174 27
EagI C'GGCC_G
Cuts at: 1907 1907
Size: 4322
EarI CTCTTCn'nnn_
Cuts at: 1022 1246 1291 2345 2555 1022
Size: 224 45 1054 210 2789
Fragments arranged by size:
2789 1054 224 210 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2530 2840 2986 4132 2530
Size: 310 146 1146 2720
Fragments arranged by size:
2720 1146 310 146
EcoICRI GAG'CTC
Cuts at: 462 1334 462
Size: 872 3450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 536 571 592 607 625 1135 1312 1330
Size: 185 35 21 15 18 510 177 18
Cuts at: 1330 2147 2579 3326 3594 351
Size: 817 432 747 268 1079
Fragments arranged by size:
1079 817 747 510 432 268 185 177
35 21 18 18 15
EcoO109I rG'GnC_Cy
Cuts at: 196 197 735 736 937 1461 196
Size: 1 538 1 201 524 3057
Fragments arranged by size:
3057 538 524 201 1 1
EcoRI G'AATT_C
Cuts at: 1326 1326
Size: 4322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 315 2341 315
Size: 2026 2296
FspI TGC'GCA
Cuts at: 2103 2103
Size: 4322
HaeII r_GCGC'y
Cuts at: 612 659 829 919 1756 2004 3026 4005 612
Size: 47 170 90 837 248 1022 979 929
Fragments arranged by size:
1022 979 929 837 248 170 90 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 652 684 2164 2194 2196 2226 652
Size: 32 1480 30 2 30 2748
Fragments arranged by size:
2748 1480 32 30 30 2
HincII GTy'rAC
Cuts at: 798 1068 1361 798
Size: 270 293 3759
Fragments arranged by size:
3759 293 270
HindIII A'AGCT_T
Cuts at: 1147 1147
Size: 4322
KasI G'GCGC_C
Cuts at: 655 915 1752 2000 655
Size: 260 837 248 2977
Fragments arranged by size:
2977 837 260 248
KpnI G_GTAC'C
Cuts at: 1342 1342
Size: 4322
MluI A'CGCG_T
Cuts at: 3755 3755
Size: 4322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1146 2993 3177 1146
Size: 1847 184 2291
Fragments arranged by size:
2291 1847 184
MscI TGG'CCA
Cuts at: 526 1104 2083 526
Size: 578 979 2765
Fragments arranged by size:
2765 979 578
MslI CAynn'nnrTG
Cuts at: 170 561 2311 2438 170
Size: 391 1750 127 2054
Fragments arranged by size:
2054 1750 391 127
MspA1I CmG'CkG
Cuts at: 647 969 1063 1433 1747 2107 3120 3365 647
Size: 322 94 370 314 360 1013 245 1604
Fragments arranged by size:
1604 1013 370 360 322 314 245 94
NaeI GCC'GGC
Cuts at: 583 2503 583
Size: 1920 2402
NarI GG'CG_CC
Cuts at: 656 916 1753 2001 656
Size: 260 837 248 2977
Fragments arranged by size:
2977 837 260 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4322
NgoMIV G'CCGG_C
Cuts at: 581 2501 581
Size: 1920 2402
NspI r_CATG'y
Cuts at: 1375 1875 2782 1375
Size: 500 907 2915
Fragments arranged by size:
2915 907 500
PasI CC'CwG_GG
Cuts at: 335 335
Size: 4322
PciI A'CATG_T
Cuts at: 2778 2778
Size: 4322
PflMI CCAn_nnn'nTGG
Cuts at: 285 1086 1165 285
Size: 801 79 3442
Fragments arranged by size:
3442 801 79
PfoI T'CCnGG_A
Cuts at: 602 602
Size: 4322
PmeI GTTT'AAAC
Cuts at: 1321 1321
Size: 4322
PmlI CAC'GTG
Cuts at: 285 349 705 285
Size: 64 356 3902
Fragments arranged by size:
3902 356 64
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 382 414 3461 3493 382
Size: 32 3047 32 1211
Fragments arranged by size:
3047 1211 32 32
PpuMI rG'GwC_Cy
Cuts at: 937 937
Size: 4322
PspOMI G'GGCC_C
Cuts at: 196 735 930 196
Size: 539 195 3588
Fragments arranged by size:
3588 539 195
PstI C_TGCA'G
Cuts at: 239 1369 2054 239
Size: 1130 685 2507
Fragments arranged by size:
2507 1130 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4322
PvuII CAG'CTG
Cuts at: 1063 1747 2107 1063
Size: 684 360 3278
Fragments arranged by size:
3278 684 360
RsrII CG'GwC_CG
Cuts at: 2517 2517
Size: 4322
SacI G_AGCT'C
Cuts at: 464 1336 464
Size: 872 3450
SalI G'TCGA_C
Cuts at: 1359 1359
Size: 4322
SapI GCTCTTCn'nnn_
Cuts at: 2345 2555 2345
Size: 210 4112
SbfI CC_TGCA'GG
Cuts at: 239 1369 239
Size: 1130 3192
ScaI AGT'ACT
Cuts at: 3839 3839
Size: 4322
SfcI C'TryA_G
Cuts at: 33 235 1279 1365 2050 3043 3234 33
Size: 202 1044 86 685 993 191 1121
Fragments arranged by size:
1121 1044 993 685 202 191 86
SfoI GGC'GCC
Cuts at: 657 917 1754 2002 657
Size: 260 837 248 2977
Fragments arranged by size:
2977 837 260 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
SmaI CCC'GGG
Cuts at: 1344 1344
Size: 4322
SmlI C'TyrA_G
Cuts at: 1477 2392 2884 3146 1477
Size: 915 492 262 2653
Fragments arranged by size:
2653 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4322
SphI G_CATG'C
Cuts at: 1375 1875 1375
Size: 500 3822
StuI AGG'CCT
Cuts at: 233 233
Size: 4322
StyI C'CwwG_G
Cuts at: 107 413 1456 1810 107
Size: 306 1043 354 2619
Fragments arranged by size:
2619 1043 354 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 376 490 660 900 1905 2038 2497 2706 376
Size: 114 170 240 1005 133 459 209 1992
Fragments arranged by size:
1992 1005 459 240 209 170 133 114
TatI w'GTAC_w
Cuts at: 3837 3837
Size: 4322
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 119 151 584 616 3839 3871 119
Size: 32 433 32 3223 32 570
Fragments arranged by size:
3223 570 433 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2119 2119
Size: 4322
XbaI T'CTAG_A
Cuts at: 183 1353 183
Size: 1170 3152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 825 1254 1817 825
Size: 429 563 3330
Fragments arranged by size:
3330 563 429
XmaI C'CCGG_G
Cuts at: 1342 1342
Size: 4322
XmnI GAAnn'nnTTC
Cuts at: 439 439
Size: 4322
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BstXI
BstZ17I CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI