Restriction map for
pF1KB5845
(Circular) (Six-base) MAPSORT of: pF1KB5845.seq Check: 9524 from: 1 to: 3530
pF1KB5845 3530 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 509 509
Size: 3530
AccI GT'mk_AC
Cuts at: 568 568
Size: 3530
Acc65I G'GTAC_C
Cuts at: 546 546
Size: 3530
AclI AA'CG_TT
Cuts at: 3370 3370
Size: 3530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 452 466 1355 1787 2534 2802 391
Size: 61 14 889 432 747 268 1119
Fragments arranged by size:
1119 889 747 432 268 61 14
AflIII A'CryG_T
Cuts at: 217 882 1986 2963 217
Size: 665 1104 977 784
Fragments arranged by size:
1104 977 784 665
AgeI A'CCGG_T
Cuts at: 1918 1918
Size: 3530
AlwNI CAG_nnn'CTG
Cuts at: 430 835 2402 430
Size: 405 1567 1558
Fragments arranged by size:
1567 1558 405
ApaI G_GGCC'C
Cuts at: 241 241
Size: 3530
ApaLI G'TGCA_C
Cuts at: 2300 2300
Size: 3530
ApoI r'AATT_y
Cuts at: 534 2883 3139 534
Size: 2349 256 925
Fragments arranged by size:
2349 925 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3530
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3530
AvaI C'yCGr_G
Cuts at: 550 550
Size: 3530
BamHI G'GATC_C
Cuts at: 555 555
Size: 3530
BanI G'GyrC_C
Cuts at: 546 960 1208 1243 546
Size: 414 248 35 2833
Fragments arranged by size:
2833 414 248 35
BanII G_rGCy'C
Cuts at: 241 544 1574 241
Size: 303 1030 2197
Fragments arranged by size:
2197 1030 303
BbeI G_GCGC'C
Cuts at: 964 1212 964
Size: 248 3282
BciVI GTATCCnnnnn_n'
Cuts at: 1428 2189 3530 1428
Size: 761 1341 1428
Fragments arranged by size:
1428 1341 761
BclI T'GATC_A
Cuts at: 1050 1050
Size: 3530
BglII A'GATC_T
Cuts at: 1045 3082 1045
Size: 2037 1493
BlpI GC'TnA_GC
Cuts at: 642 642
Size: 3530
Bme1580I G_kGCm'C
Cuts at: 241 355 1155 1248 2304 241
Size: 114 800 93 1056 1467
Fragments arranged by size:
1467 1056 800 114 93
BmrI ACTGGGnnnn_n'
Cuts at: 240 742 823 1158 3302 240
Size: 502 81 335 2144 468
Fragments arranged by size:
2144 502 468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1897 1897
Size: 3530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 303 280
Size: 23 3507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1585 2077 2375 706
Size: 879 492 298 1861
Fragments arranged by size:
1861 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3234 3234
Size: 3530
BsaAI yAC'GTr
Cuts at: 883 883
Size: 3530
BsaBI GATnn'nnATC
Cuts at: 6 1044 3081 6
Size: 1038 2037 455
Fragments arranged by size:
2037 1038 455
BsaHI Gr'CG_yC
Cuts at: 961 1209 1911 3407 961
Size: 248 702 1496 1084
Fragments arranged by size:
1496 1084 702 248
BsaWI w'CCGG_w
Cuts at: 719 1240 1918 2192 2339 2750 719
Size: 521 678 274 147 411 1499
Fragments arranged by size:
1499 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 226
Size: 3530
BseYI C'CCAG_C
Cuts at: 269 287 955 1713 2290 2786 269
Size: 18 668 758 577 496 1013
Fragments arranged by size:
1013 758 668 577 496 18
BsiEI CG_ry'CG
Cuts at: 104 1072 1118 2326 104
Size: 968 46 1208 1308
Fragments arranged by size:
1308 1208 968 46
BsiHKAI G_wGCw'C
Cuts at: 544 1322 1512 2304 544
Size: 778 190 792 1770
Fragments arranged by size:
1770 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 241 355 544 1155 1248 1322 1512 1574 2304
Size: 114 189 611 93 74 190 62 730
Cuts at: 2304 241
Size: 1467
Fragments arranged by size:
1467 730 611 190 189 114 93 74
62
BspEI T'CCGG_A
Cuts at: 2750 2750
Size: 3530
BspHI T'CATG_A
Cuts at: 2706 2706
Size: 3530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 580 794 1096 1477 509
Size: 71 214 302 381 2562
Fragments arranged by size:
2562 381 302 214 71
BsrBI CCG'CTC
Cuts at: 50 1822 1876 3360 3525 50
Size: 1772 54 1484 165 55
Fragments arranged by size:
1772 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1442 1442
Size: 3530
BsrFI r'CCGG_y
Cuts at: 440 1528 1709 1918 440
Size: 1088 181 209 2052
Fragments arranged by size:
2052 1088 209 181
BssSI C'ACGA_G
Cuts at: 1801 2159 1801
Size: 358 3172
BstAPI GCAn_nnn'nTGC
Cuts at: 907 907
Size: 3530
BstBI TT'CG_AA
Cuts at: 1891 1891
Size: 3530
BstYI r'GATC_y
Cuts at: 1 296 555 1022 1045 1380 1626 2638 2724
Size: 295 259 467 23 335 246 1012 86
Cuts at: 2724 2736 3082 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 295 259 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 389 389
Size: 3530
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1480 1661 1824 1480
Size: 181 163 3186
Fragments arranged by size:
3186 181 163
BtsI GCAGTG_nn'
Cuts at: 773 773
Size: 3530
DraI TTT'AAA
Cuts at: 529 529
Size: 3530
DrdI GACnn_nn'nnGTC
Cuts at: 1236 2094 1236
Size: 858 2672
EaeI y'GGCC_r
Cuts at: 480 1115 1289 1680 1707 3052 480
Size: 635 174 391 27 1345 958
Fragments arranged by size:
1345 958 635 391 174 27
EagI C'GGCC_G
Cuts at: 1115 1115
Size: 3530
EarI CTCTTCn'nnn_
Cuts at: 1553 1763 1553
Size: 210 3320
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1738 2048 2194 3340 1738
Size: 310 146 1146 1928
Fragments arranged by size:
1928 1146 310 146
EcoICRI GAG'CTC
Cuts at: 542 542
Size: 3530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 303 391 452 466 1355 1787 2534 2802
Size: 23 88 61 14 889 432 747 268
Cuts at: 2802 280
Size: 1008
Fragments arranged by size:
1008 889 747 432 268 88 61 23
14
EcoO109I rG'GnC_Cy
Cuts at: 237 238 669 237
Size: 1 431 3098
Fragments arranged by size:
3098 431 1
EcoRI G'AATT_C
Cuts at: 534 534
Size: 3530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1549 1549
Size: 3530
FspI TGC'GCA
Cuts at: 1311 1311
Size: 3530
HaeII r_GCGC'y
Cuts at: 964 1212 2234 3213 964
Size: 248 1022 979 1281
Fragments arranged by size:
1281 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 417 1372 1402 1404 1434 385
Size: 32 955 30 2 30 2481
Fragments arranged by size:
2481 955 32 30 30 2
HincII GTy'rAC
Cuts at: 569 569
Size: 3530
KasI G'GCGC_C
Cuts at: 960 1208 960
Size: 248 3282
KpnI G_GTAC'C
Cuts at: 550 550
Size: 3530
MluI A'CGCG_T
Cuts at: 2963 2963
Size: 3530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 202 2201 2385 202
Size: 1999 184 1347
Fragments arranged by size:
1999 1347 184
MscI TGG'CCA
Cuts at: 1291 1291
Size: 3530
MslI CAynn'nnrTG
Cuts at: 1519 1646 1519
Size: 127 3403
MspA1I CmG'CkG
Cuts at: 291 427 641 955 1315 2328 2573 291
Size: 136 214 314 360 1013 245 1248
Fragments arranged by size:
1248 1013 360 314 245 214 136
NaeI GCC'GGC
Cuts at: 1711 1711
Size: 3530
NarI GG'CG_CC
Cuts at: 961 1209 961
Size: 248 3282
NgoMIV G'CCGG_C
Cuts at: 1709 1709
Size: 3530
NspI r_CATG'y
Cuts at: 221 583 1083 1990 221
Size: 362 500 907 1761
Fragments arranged by size:
1761 907 500 362
PciI A'CATG_T
Cuts at: 217 1986 217
Size: 1769 1761
PflMI CCAn_nnn'nTGG
Cuts at: 120 120
Size: 3530
PmeI GTTT'AAAC
Cuts at: 529 529
Size: 3530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2701 2669
Size: 32 3498
PspOMI G'GGCC_C
Cuts at: 237 237
Size: 3530
PstI C_TGCA'G
Cuts at: 296 577 1262 296
Size: 281 685 2564
Fragments arranged by size:
2564 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3530
PvuII CAG'CTG
Cuts at: 291 427 955 1315 291
Size: 136 528 360 2506
Fragments arranged by size:
2506 528 360 136
RsrII CG'GwC_CG
Cuts at: 1725 1725
Size: 3530
SacI G_AGCT'C
Cuts at: 544 544
Size: 3530
SalI G'TCGA_C
Cuts at: 567 567
Size: 3530
SapI GCTCTTCn'nnn_
Cuts at: 1553 1763 1553
Size: 210 3320
SbfI CC_TGCA'GG
Cuts at: 577 577
Size: 3530
ScaI AGT'ACT
Cuts at: 3047 3047
Size: 3530
SfcI C'TryA_G
Cuts at: 33 292 573 1258 2251 2442 33
Size: 259 281 685 993 191 1121
Fragments arranged by size:
1121 993 685 281 259 191
SfoI GGC'GCC
Cuts at: 962 1210 962
Size: 248 3282
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3530
SmaI CCC'GGG
Cuts at: 552 552
Size: 3530
SmlI C'TyrA_G
Cuts at: 685 1600 2092 2354 685
Size: 915 492 262 1861
Fragments arranged by size:
1861 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3530
SphI G_CATG'C
Cuts at: 583 1083 583
Size: 500 3030
StyI C'CwwG_G
Cuts at: 664 1018 664
Size: 354 3176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 240 1113 1246 1705 1914 240
Size: 873 133 459 209 1856
Fragments arranged by size:
1856 873 459 209 133
TatI w'GTAC_w
Cuts at: 3045 3045
Size: 3530
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 3498
Tth111I GACn'n_nGTC
Cuts at: 1327 1327
Size: 3530
XbaI T'CTAG_A
Cuts at: 561 561
Size: 3530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 307 1025 307
Size: 718 2812
XmaI C'CCGG_G
Cuts at: 550 550
Size: 3530
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI