Restriction map for
pF1KB5848
(Circular) (Six-base) MAPSORT of: pF1KB5848.seq Check: 4571 from: 1 to: 4571
pF1KB5848 4571 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 345 1352 1609 345
Size: 1007 257 3307
Fragments arranged by size:
3307 1007 257
Acc65I G'GTAC_C
Cuts at: 1587 1587
Size: 4571
AclI AA'CG_TT
Cuts at: 4411 4411
Size: 4571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 2396 2828 3575 3843 748
Size: 1648 432 747 268 1476
Fragments arranged by size:
1648 1476 747 432 268
AfeI AGC'GCT
Cuts at: 217 217
Size: 4571
AflIII A'CryG_T
Cuts at: 1923 3027 4004 1923
Size: 1104 977 2490
Fragments arranged by size:
2490 1104 977
AgeI A'CCGG_T
Cuts at: 2959 2959
Size: 4571
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 707 739 707
Size: 32 4539
AlwNI CAG_nnn'CTG
Cuts at: 684 1005 1876 3443 684
Size: 321 871 1567 1812
Fragments arranged by size:
1812 1567 871 321
ApaLI G'TGCA_C
Cuts at: 3341 3341
Size: 4571
ApoI r'AATT_y
Cuts at: 598 667 746 757 1054 1277 1310 1575 3924
Size: 69 79 11 297 223 33 265 2349
Cuts at: 3924 4180 598
Size: 256 989
Fragments arranged by size:
2349 989 297 265 256 223 79 69
33 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 4571
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
AvaI C'yCGr_G
Cuts at: 1591 1591
Size: 4571
BamHI G'GATC_C
Cuts at: 1596 1596
Size: 4571
BanI G'GyrC_C
Cuts at: 1587 2001 2249 2284 1587
Size: 414 248 35 3874
Fragments arranged by size:
3874 414 248 35
BanII G_rGCy'C
Cuts at: 229 625 1585 2615 229
Size: 396 960 1030 2185
Fragments arranged by size:
2185 1030 960 396
BbeI G_GCGC'C
Cuts at: 2005 2253 2005
Size: 248 4323
BbsI GAAGACnn'nnnn_
Cuts at: 736 736
Size: 4571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1377 1411 1377
Size: 34 4537
BciVI GTATCCnnnnn_n'
Cuts at: 1197 2469 3230 4571 1197
Size: 1272 761 1341 1197
Fragments arranged by size:
1341 1272 1197 761
BclI T'GATC_A
Cuts at: 485 660 1037 2091 485
Size: 175 377 1054 2965
Fragments arranged by size:
2965 1054 377 175
BfrBI ATG'CAT
Cuts at: 945 945
Size: 4571
BglI GCCn_nnn'nGGC
Cuts at: 154 154
Size: 4571
BglII A'GATC_T
Cuts at: 2086 4123 2086
Size: 2037 2534
BlpI GC'TnA_GC
Cuts at: 977 1683 977
Size: 706 3865
Bme1580I G_kGCm'C
Cuts at: 2196 2289 3345 2196
Size: 93 1056 3422
Fragments arranged by size:
3422 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1783 1864 2199 4343 1783
Size: 81 335 2144 2011
Fragments arranged by size:
2144 2011 335 81
BmtI G_CTAG'C
Cuts at: 985 985
Size: 4571
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2938 2938
Size: 4571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1027 706
Size: 321 4250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 627 1747 2626 3118 3416 583
Size: 44 1120 879 492 298 1738
Fragments arranged by size:
1738 1120 879 492 298 44
BsaI GGTCTCn'nnnn_
Cuts at: 4275 4275
Size: 4571
BsaAI yAC'GTr
Cuts at: 1924 1924
Size: 4571
BsaBI GATnn'nnATC
Cuts at: 6 536 2085 4122 6
Size: 530 1549 2037 455
Fragments arranged by size:
2037 1549 530 455
BsaHI Gr'CG_yC
Cuts at: 2002 2250 2952 4448 2002
Size: 248 702 1496 2125
Fragments arranged by size:
2125 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1760 2281 2959 3233 3380 3791 1760
Size: 521 678 274 147 411 2540
Fragments arranged by size:
2540 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 498 528 665 695 498
Size: 30 137 30 4374
Fragments arranged by size:
4374 137 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1276 1498 1276
Size: 222 4349
BseYI C'CCAG_C
Cuts at: 278 1996 2754 3331 3827 278
Size: 1718 758 577 496 1022
Fragments arranged by size:
1718 1022 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 724 250
Size: 474 4097
BsiEI CG_ry'CG
Cuts at: 104 2113 2159 3367 104
Size: 2009 46 1208 1308
Fragments arranged by size:
2009 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1585 2363 2553 3345 1585
Size: 778 190 792 2811
Fragments arranged by size:
2811 792 778 190
BsmI GAATG_Cn'
Cuts at: 775 775
Size: 4571
Bsp1286I G_dGCh'C
Cuts at: 229 625 1585 2196 2289 2363 2553 2615 3345
Size: 396 960 611 93 74 190 62 730
Cuts at: 3345 229
Size: 1455
Fragments arranged by size:
1455 960 730 611 396 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3791 3791
Size: 4571
BspHI T'CATG_A
Cuts at: 3747 3747
Size: 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1078 1621 1835 2137 2518 1078
Size: 543 214 302 381 3131
Fragments arranged by size:
3131 543 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2863 2917 4401 4566 50
Size: 2813 54 1484 165 55
Fragments arranged by size:
2813 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 641 889 2483 641
Size: 248 1594 2729
Fragments arranged by size:
2729 1594 248
BsrFI r'CCGG_y
Cuts at: 126 2569 2750 2959 126
Size: 2443 181 209 1738
Fragments arranged by size:
2443 1738 209 181
BsrGI T'GTAC_A
Cuts at: 996 1304 996
Size: 308 4263
BssSI C'ACGA_G
Cuts at: 2842 3200 2842
Size: 358 4213
BstAPI GCAn_nnn'nTGC
Cuts at: 273 1948 273
Size: 1675 2896
BstBI TT'CG_AA
Cuts at: 2932 2932
Size: 4571
BstYI r'GATC_y
Cuts at: 1 705 1596 2063 2086 2421 2667 3679 3765
Size: 704 891 467 23 335 246 1012 86
Cuts at: 3765 3777 4123 1
Size: 12 346 449
Fragments arranged by size:
1012 891 704 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 346 1353 346
Size: 1007 3564
Bsu36I CC'TnA_GG
Cuts at: 808 808
Size: 4571
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2521 2702 2865 2521
Size: 181 163 4227
Fragments arranged by size:
4227 181 163
BtsI GCAGTG_nn'
Cuts at: 343 1814 343
Size: 1471 3100
DraI TTT'AAA
Cuts at: 1570 1570
Size: 4571
DrdI GACnn_nn'nnGTC
Cuts at: 2277 3135 2277
Size: 858 3713
EaeI y'GGCC_r
Cuts at: 1060 2156 2330 2721 2748 4093 1060
Size: 1096 174 391 27 1345 1538
Fragments arranged by size:
1538 1345 1096 391 174 27
EagI C'GGCC_G
Cuts at: 2156 2156
Size: 4571
EarI CTCTTCn'nnn_
Cuts at: 376 539 1148 2594 2804 376
Size: 163 609 1446 210 2143
Fragments arranged by size:
2143 1446 609 210 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2779 3089 3235 4381 2779
Size: 310 146 1146 2969
Fragments arranged by size:
2969 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1583 1583
Size: 4571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 748 1027 2396 2828 3575 3843 706
Size: 42 279 1369 432 747 268 1434
Fragments arranged by size:
1434 1369 747 432 279 268 42
EcoO109I rG'GnC_Cy
Cuts at: 1710 1710
Size: 4571
EcoRI G'AATT_C
Cuts at: 598 1575 598
Size: 977 3594
EcoRV GAT'ATC
Cuts at: 1175 1175
Size: 4571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1177 2590 1177
Size: 1413 3158
FspI TGC'GCA
Cuts at: 2352 2352
Size: 4571
HaeII r_GCGC'y
Cuts at: 146 219 2005 2253 3275 4254 146
Size: 73 1786 248 1022 979 463
Fragments arranged by size:
1786 1022 979 463 248 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 764 796 2413 2443 2445 2475 764
Size: 32 1617 30 2 30 2860
Fragments arranged by size:
2860 1617 32 30 30 2
HincII GTy'rAC
Cuts at: 1610 1610
Size: 4571
HindIII A'AGCT_T
Cuts at: 1390 1390
Size: 4571
KasI G'GCGC_C
Cuts at: 2001 2249 2001
Size: 248 4323
KpnI G_GTAC'C
Cuts at: 1591 1591
Size: 4571
MluI A'CGCG_T
Cuts at: 4004 4004
Size: 4571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1357 3242 3426 1357
Size: 1885 184 2502
Fragments arranged by size:
2502 1885 184
MscI TGG'CCA
Cuts at: 1062 2332 1062
Size: 1270 3301
MslI CAynn'nnrTG
Cuts at: 836 2560 2687 836
Size: 1724 127 2720
Fragments arranged by size:
2720 1724 127
MspA1I CmG'CkG
Cuts at: 273 1682 1996 2356 3369 3614 273
Size: 1409 314 360 1013 245 1230
Fragments arranged by size:
1409 1230 1013 360 314 245
NaeI GCC'GGC
Cuts at: 128 2752 128
Size: 2624 1947
NarI GG'CG_CC
Cuts at: 2002 2250 2002
Size: 248 4323
NgoMIV G'CCGG_C
Cuts at: 126 2750 126
Size: 2624 1947
NheI G'CTAG_C
Cuts at: 981 981
Size: 4571
NsiI A_TGCA'T
Cuts at: 947 947
Size: 4571
NspI r_CATG'y
Cuts at: 1536 1624 2124 3031 1536
Size: 88 500 907 3076
Fragments arranged by size:
3076 907 500 88
PasI CC'CwG_GG
Cuts at: 222 222
Size: 4571
PciI A'CATG_T
Cuts at: 3027 3027
Size: 4571
PfoI T'CCnGG_A
Cuts at: 683 1004 683
Size: 321 4250
PmeI GTTT'AAAC
Cuts at: 1570 1570
Size: 4571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4539
PstI C_TGCA'G
Cuts at: 1075 1618 2303 1075
Size: 543 685 3343
Fragments arranged by size:
3343 685 543
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4571
PvuII CAG'CTG
Cuts at: 1996 2356 1996
Size: 360 4211
RsrII CG'GwC_CG
Cuts at: 192 2766 192
Size: 2574 1997
SacI G_AGCT'C
Cuts at: 1585 1585
Size: 4571
SalI G'TCGA_C
Cuts at: 1608 1608
Size: 4571
SapI GCTCTTCn'nnn_
Cuts at: 376 1148 2594 2804 376
Size: 772 1446 210 2143
Fragments arranged by size:
2143 1446 772 210
SbfI CC_TGCA'GG
Cuts at: 1618 1618
Size: 4571
ScaI AGT'ACT
Cuts at: 4088 4088
Size: 4571
SfcI C'TryA_G
Cuts at: 33 1071 1614 2299 3292 3483 33
Size: 1038 543 685 993 191 1121
Fragments arranged by size:
1121 1038 993 685 543 191
SfoI GGC'GCC
Cuts at: 2003 2251 2003
Size: 248 4323
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
SgrAI Cr'CCGG_yG
Cuts at: 126 126
Size: 4571
SmaI CCC'GGG
Cuts at: 1593 1593
Size: 4571
SmlI C'TyrA_G
Cuts at: 562 606 1726 2641 3133 3395 562
Size: 44 1120 915 492 262 1738
Fragments arranged by size:
1738 1120 915 492 262 44
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4571
SphI G_CATG'C
Cuts at: 1624 2124 1624
Size: 500 4071
SspI AAT'ATT
Cuts at: 863 863
Size: 4571
StuI AGG'CCT
Cuts at: 568 568
Size: 4571
StyI C'CwwG_G
Cuts at: 1705 2059 1705
Size: 354 4217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 180 2154 2287 2746 2955 180
Size: 1974 133 459 209 1796
Fragments arranged by size:
1974 1796 459 209 133
TatI w'GTAC_w
Cuts at: 996 1223 1304 4086 996
Size: 227 81 2782 1481
Fragments arranged by size:
2782 1481 227 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4088 4120 4088
Size: 32 4539
Tth111I GACn'n_nGTC
Cuts at: 2368 2368
Size: 4571
XbaI T'CTAG_A
Cuts at: 1506 1602 1506
Size: 96 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1344 2066 1344
Size: 722 3849
XmaI C'CCGG_G
Cuts at: 1591 1591
Size: 4571
XmnI GAAnn'nnTTC
Cuts at: 1244 1244
Size: 4571
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NsiI NspI PasI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BmgBI Bpu10I BsiWI BsmBI BssHII
BstEII BstXI BtgI ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NcoI NdeI NotI NruI PacI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SwaI XhoI
ZraI