Restriction map for
pF1KB5849
(Circular) (Six-base) MAPSORT of: pF1KB5849.seq Check: 8281 from: 1 to: 3851
pF1KB5849 3851 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 889 889
Size: 3851
Acc65I G'GTAC_C
Cuts at: 867 867
Size: 3851
AclI AA'CG_TT
Cuts at: 3691 3691
Size: 3851
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1676 2108 2855 3123 1676
Size: 432 747 268 2404
Fragments arranged by size:
2404 747 432 268
AfeI AGC'GCT
Cuts at: 745 745
Size: 3851
AflIII A'CryG_T
Cuts at: 1203 2307 3284 1203
Size: 1104 977 1770
Fragments arranged by size:
1770 1104 977
AgeI A'CCGG_T
Cuts at: 2239 2239
Size: 3851
AleI CACnn'nnGTG
Cuts at: 391 391
Size: 3851
AlwNI CAG_nnn'CTG
Cuts at: 192 289 1156 2723 192
Size: 97 867 1567 1320
Fragments arranged by size:
1567 1320 867 97
ApaLI G'TGCA_C
Cuts at: 2621 2621
Size: 3851
ApoI r'AATT_y
Cuts at: 855 3204 3460 855
Size: 2349 256 1246
Fragments arranged by size:
2349 1246 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
AvaI C'yCGr_G
Cuts at: 612 703 871 612
Size: 91 168 3592
Fragments arranged by size:
3592 168 91
BamHI G'GATC_C
Cuts at: 876 876
Size: 3851
BanI G'GyrC_C
Cuts at: 676 867 1281 1529 1564 676
Size: 191 414 248 35 2963
Fragments arranged by size:
2963 414 248 191 35
BanII G_rGCy'C
Cuts at: 556 695 704 710 865 1895 556
Size: 139 9 6 155 1030 2512
Fragments arranged by size:
2512 1030 155 139 9 6
BbeI G_GCGC'C
Cuts at: 1285 1533 1285
Size: 248 3603
BbvCI CC'TCA_GC
Cuts at: 362 362
Size: 3851
BciVI GTATCCnnnnn_n'
Cuts at: 1749 2510 3851 1749
Size: 761 1341 1749
Fragments arranged by size:
1749 1341 761
BclI T'GATC_A
Cuts at: 395 1371 395
Size: 976 2875
BglII A'GATC_T
Cuts at: 1366 3403 1366
Size: 2037 1814
BlpI GC'TnA_GC
Cuts at: 963 963
Size: 3851
Bme1580I G_kGCm'C
Cuts at: 679 1476 1569 2625 679
Size: 797 93 1056 1905
Fragments arranged by size:
1905 1056 797 93
BmrI ACTGGGnnnn_n'
Cuts at: 423 1063 1144 1479 3623 423
Size: 640 81 335 2144 651
Fragments arranged by size:
2144 651 640 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 570 2218 570
Size: 1648 2203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 217 166
Size: 51 3800
Bpu10I CC'TnA_GC
Cuts at: 362 362
Size: 3851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1906 2398 2696 1027
Size: 879 492 298 2182
Fragments arranged by size:
2182 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3555 3555
Size: 3851
BsaAI yAC'GTr
Cuts at: 1204 1204
Size: 3851
BsaBI GATnn'nnATC
Cuts at: 6 1365 3402 6
Size: 1359 2037 455
Fragments arranged by size:
2037 1359 455
BsaHI Gr'CG_yC
Cuts at: 1282 1530 2232 3728 1282
Size: 248 702 1496 1405
Fragments arranged by size:
1496 1405 702 248
BsaWI w'CCGG_w
Cuts at: 410 1040 1561 2239 2513 2660 3071 410
Size: 630 521 678 274 147 411 1190
Fragments arranged by size:
1190 678 630 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 351 562 351
Size: 211 3640
BseYI C'CCAG_C
Cuts at: 238 514 1276 2034 2611 3107 238
Size: 276 762 758 577 496 982
Fragments arranged by size:
982 762 758 577 496 276
BsiEI CG_ry'CG
Cuts at: 104 411 1393 1439 2647 104
Size: 307 982 46 1208 1308
Fragments arranged by size:
1308 1208 982 307 46
BsiHKAI G_wGCw'C
Cuts at: 865 1643 1833 2625 865
Size: 778 190 792 2091
Fragments arranged by size:
2091 792 778 190
BsmI GAATG_Cn'
Cuts at: 511 511
Size: 3851
Bsp1286I G_dGCh'C
Cuts at: 556 679 695 704 710 865 1476 1569 1643
Size: 123 16 9 6 155 611 93 74
Cuts at: 1643 1833 1895 2625 556
Size: 190 62 730 1782
Fragments arranged by size:
1782 730 611 190 155 123 93 74
62 16 9 6
BspEI T'CCGG_A
Cuts at: 3071 3071
Size: 3851
BspHI T'CATG_A
Cuts at: 3027 3027
Size: 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 901 1115 1417 1798 901
Size: 214 302 381 2954
Fragments arranged by size:
2954 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2143 2197 3681 3846 50
Size: 2093 54 1484 165 55
Fragments arranged by size:
2093 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1763 1763
Size: 3851
BsrFI r'CCGG_y
Cuts at: 1849 2030 2239 1849
Size: 181 209 3461
Fragments arranged by size:
3461 209 181
BsrGI T'GTAC_A
Cuts at: 587 587
Size: 3851
BssSI C'ACGA_G
Cuts at: 2122 2480 2122
Size: 358 3493
BstAPI GCAn_nnn'nTGC
Cuts at: 192 1228 192
Size: 1036 2815
BstBI TT'CG_AA
Cuts at: 2212 2212
Size: 3851
BstEII G'GTnAC_C
Cuts at: 561 561
Size: 3851
BstYI r'GATC_y
Cuts at: 1 637 818 876 1343 1366 1701 1947 2959
Size: 636 181 58 467 23 335 246 1012
Cuts at: 2959 3045 3057 3403 1
Size: 86 12 346 449
Fragments arranged by size:
1012 636 467 449 346 335 246 181
86 58 23 12
Bsu36I CC'TnA_GG
Cuts at: 539 539
Size: 3851
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 264 1801 1982 2145 264
Size: 1537 181 163 1970
Fragments arranged by size:
1970 1537 181 163
BtsI GCAGTG_nn'
Cuts at: 193 655 1094 193
Size: 462 439 2950
Fragments arranged by size:
2950 462 439
DraI TTT'AAA
Cuts at: 850 850
Size: 3851
DrdI GACnn_nn'nnGTC
Cuts at: 1557 2415 1557
Size: 858 2993
EaeI y'GGCC_r
Cuts at: 152 1436 1610 2001 2028 3373 152
Size: 1284 174 391 27 1345 630
Fragments arranged by size:
1345 1284 630 391 174 27
EagI C'GGCC_G
Cuts at: 1436 1436
Size: 3851
EarI CTCTTCn'nnn_
Cuts at: 196 328 1874 2084 196
Size: 132 1546 210 1963
Fragments arranged by size:
1963 1546 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 748 2059 2369 2515 3661 748
Size: 1311 310 146 1146 938
Fragments arranged by size:
1311 1146 938 310 146
EcoICRI GAG'CTC
Cuts at: 863 863
Size: 3851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 217 1676 2108 2855 3123 166
Size: 51 1459 432 747 268 894
Fragments arranged by size:
1459 894 747 432 268 51
EcoO109I rG'GnC_Cy
Cuts at: 990 990
Size: 3851
EcoRI G'AATT_C
Cuts at: 855 855
Size: 3851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3851
FspI TGC'GCA
Cuts at: 478 1632 478
Size: 1154 2697
HaeII r_GCGC'y
Cuts at: 747 1285 1533 2555 3534 747
Size: 538 248 1022 979 1064
Fragments arranged by size:
1064 1022 979 538 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 629 661 679 711 1693 1723 1725 1755 629
Size: 32 18 32 982 30 2 30 2725
Fragments arranged by size:
2725 982 32 32 30 30 18 2
HincII GTy'rAC
Cuts at: 890 890
Size: 3851
KasI G'GCGC_C
Cuts at: 1281 1529 1281
Size: 248 3603
KpnI G_GTAC'C
Cuts at: 871 871
Size: 3851
MluI A'CGCG_T
Cuts at: 3284 3284
Size: 3851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 407 2522 2706 407
Size: 2115 184 1552
Fragments arranged by size:
2115 1552 184
MscI TGG'CCA
Cuts at: 154 1612 154
Size: 1458 2393
MslI CAynn'nnrTG
Cuts at: 391 1840 1967 391
Size: 1449 127 2275
Fragments arranged by size:
2275 1449 127
MspA1I CmG'CkG
Cuts at: 324 534 962 1276 1636 2649 2894 324
Size: 210 428 314 360 1013 245 1281
Fragments arranged by size:
1281 1013 428 360 314 245 210
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 3851
NarI GG'CG_CC
Cuts at: 1282 1530 1282
Size: 248 3603
NdeI CA'TA_TG
Cuts at: 390 390
Size: 3851
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 3851
NspI r_CATG'y
Cuts at: 741 904 1404 2311 741
Size: 163 500 907 2281
Fragments arranged by size:
2281 907 500 163
PciI A'CATG_T
Cuts at: 2307 2307
Size: 3851
PmeI GTTT'AAAC
Cuts at: 850 850
Size: 3851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3819
PstI C_TGCA'G
Cuts at: 637 662 898 1583 637
Size: 25 236 685 2905
Fragments arranged by size:
2905 685 236 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3851
PvuII CAG'CTG
Cuts at: 1276 1636 1276
Size: 360 3491
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 3851
SacI G_AGCT'C
Cuts at: 865 865
Size: 3851
SalI G'TCGA_C
Cuts at: 888 888
Size: 3851
SapI GCTCTTCn'nnn_
Cuts at: 1874 2084 1874
Size: 210 3641
SbfI CC_TGCA'GG
Cuts at: 898 898
Size: 3851
ScaI AGT'ACT
Cuts at: 3368 3368
Size: 3851
SfcI C'TryA_G
Cuts at: 33 633 658 894 1579 2572 2763 33
Size: 600 25 236 685 993 191 1121
Fragments arranged by size:
1121 993 685 600 236 191 25
SfoI GGC'GCC
Cuts at: 1283 1531 1283
Size: 248 3603
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
SmaI CCC'GGG
Cuts at: 614 873 614
Size: 259 3592
SmlI C'TyrA_G
Cuts at: 1006 1921 2413 2675 1006
Size: 915 492 262 2182
Fragments arranged by size:
2182 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3851
SphI G_CATG'C
Cuts at: 904 1404 904
Size: 500 3351
StuI AGG'CCT
Cuts at: 778 778
Size: 3851
StyI C'CwwG_G
Cuts at: 985 1339 985
Size: 354 3497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 618 1434 1567 2026 2235 274
Size: 344 816 133 459 209 1890
Fragments arranged by size:
1890 816 459 344 209 133
TatI w'GTAC_w
Cuts at: 587 3366 587
Size: 2779 1072
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 3819
Tth111I GACn'n_nGTC
Cuts at: 1648 1648
Size: 3851
XbaI T'CTAG_A
Cuts at: 882 882
Size: 3851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1346 1346
Size: 3851
XmaI C'CCGG_G
Cuts at: 612 871 612
Size: 259 3592
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmBI BssHII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI