Restriction map for
pF1KB5853
(Circular) (Six-base) MAPSORT of: pF1KB5853.seq Check: 8748 from: 1 to: 4079
pF1KB5853 4079 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 904 983 1117 904
Size: 79 134 3866
Fragments arranged by size:
3866 134 79
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4079
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1904 2336 3083 3351 1904
Size: 432 747 268 2632
Fragments arranged by size:
2632 747 432 268
AfeI AGC'GCT
Cuts at: 376 376
Size: 4079
AflIII A'CryG_T
Cuts at: 1431 2535 3512 1431
Size: 1104 977 1998
Fragments arranged by size:
1998 1104 977
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AhdI GACnn_n'nnGTC
Cuts at: 282 282
Size: 4079
AleI CACnn'nnGTG
Cuts at: 366 499 366
Size: 133 3946
AlwNI CAG_nnn'CTG
Cuts at: 1384 2951 1384
Size: 1567 2512
ApaLI G'TGCA_C
Cuts at: 2849 2849
Size: 4079
ApoI r'AATT_y
Cuts at: 1083 3432 3688 1083
Size: 2349 256 1474
Fragments arranged by size:
2349 1474 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 1099 1099
Size: 4079
AvrII C'CTAG_G
Cuts at: 849 849
Size: 4079
BamHI G'GATC_C
Cuts at: 1104 1104
Size: 4079
BanI G'GyrC_C
Cuts at: 1095 1509 1757 1792 1095
Size: 414 248 35 3382
Fragments arranged by size:
3382 414 248 35
BanII G_rGCy'C
Cuts at: 452 1093 2123 452
Size: 641 1030 2408
Fragments arranged by size:
2408 1030 641
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3831
BbvCI CC'TCA_GC
Cuts at: 307 307
Size: 4079
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4079
BglII A'GATC_T
Cuts at: 1594 3631 1594
Size: 2037 2042
BlpI GC'TnA_GC
Cuts at: 1191 1191
Size: 4079
Bme1580I G_kGCm'C
Cuts at: 1704 1797 2853 1704
Size: 93 1056 2930
Fragments arranged by size:
2930 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 384 1291 1372 1707 3851 384
Size: 907 81 335 2144 612
Fragments arranged by size:
2144 907 612 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 4079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 634
Size: 4079
Bpu10I CC'TnA_GC
Cuts at: 307 1039 307
Size: 732 3347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 2134 2626 2924 1255
Size: 879 492 298 2410
Fragments arranged by size:
2410 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1029 3783 1029
Size: 2754 1325
BsaAI yAC'GTr
Cuts at: 1432 1432
Size: 4079
BsaBI GATnn'nnATC
Cuts at: 6 1593 3630 6
Size: 1587 2037 455
Fragments arranged by size:
2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 1510 1758 2460 3956 1510
Size: 248 702 1496 1633
Fragments arranged by size:
1633 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1268 1789 2467 2741 2888 3299 1268
Size: 521 678 274 147 411 2048
Fragments arranged by size:
2048 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 254 464 568 579 1504 2262 2839 3335 254
Size: 210 104 11 925 758 577 496 998
Fragments arranged by size:
998 925 758 577 496 210 104 11
BsiEI CG_ry'CG
Cuts at: 104 1621 1667 2875 104
Size: 1517 46 1208 1308
Fragments arranged by size:
1517 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 125 1093 1871 2061 2853 125
Size: 968 778 190 792 1351
Fragments arranged by size:
1351 968 792 778 190
BsmI GAATG_Cn'
Cuts at: 232 232
Size: 4079
Bsp1286I G_dGCh'C
Cuts at: 125 452 1093 1704 1797 1871 2061 2123 2853
Size: 327 641 611 93 74 190 62 730
Cuts at: 2853 125
Size: 1351
Fragments arranged by size:
1351 730 641 611 327 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3299 3299
Size: 4079
BspHI T'CATG_A
Cuts at: 3255 3255
Size: 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1129 1343 1645 2026 1129
Size: 214 302 381 3182
Fragments arranged by size:
3182 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3909 4074 50
Size: 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 781 1991 781
Size: 1210 2869
BsrFI r'CCGG_y
Cuts at: 2077 2258 2467 2077
Size: 181 209 3689
Fragments arranged by size:
3689 209 181
BssSI C'ACGA_G
Cuts at: 2350 2708 2350
Size: 358 3721
BstAPI GCAn_nnn'nTGC
Cuts at: 888 1456 888
Size: 568 3511
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 342 342
Size: 4079
BstYI r'GATC_y
Cuts at: 1 1013 1104 1571 1594 1929 2175 3187 3273
Size: 1012 91 467 23 335 246 1012 86
Cuts at: 3273 3285 3631 1
Size: 12 346 449
Fragments arranged by size:
1012 1012 467 449 346 335 246 91
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 355 355
Size: 4079
BtgI C'CryG_G
Cuts at: 107 107
Size: 4079
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 232 2029 2210 2373 232
Size: 1797 181 163 1938
Fragments arranged by size:
1938 1797 181 163
BtsI GCAGTG_nn'
Cuts at: 227 574 1322 227
Size: 347 748 2984
Fragments arranged by size:
2984 748 347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 678 713 941 976 678
Size: 35 228 35 3781
Fragments arranged by size:
3781 228 35 35
DraI TTT'AAA
Cuts at: 1078 1078
Size: 4079
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 597 656 1664 1838 2229 2256 3601 597
Size: 59 1008 174 391 27 1345 1075
Fragments arranged by size:
1345 1075 1008 391 174 59 27
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4079
EarI CTCTTCn'nnn_
Cuts at: 928 2102 2312 928
Size: 1174 210 2695
Fragments arranged by size:
2695 1174 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2287 2597 2743 3889 2287
Size: 310 146 1146 2477
Fragments arranged by size:
2477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1091 1091
Size: 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1904 2336 3083 3351 634
Size: 1270 432 747 268 1362
Fragments arranged by size:
1362 1270 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 310 310
Size: 4079
EcoO109I rG'GnC_Cy
Cuts at: 258 302 416 513 1218 258
Size: 44 114 97 705 3119
Fragments arranged by size:
3119 705 114 97 44
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 4079
FspI TGC'GCA
Cuts at: 1860 1860
Size: 4079
HaeII r_GCGC'y
Cuts at: 378 1513 1761 2783 3762 378
Size: 1135 248 1022 979 695
Fragments arranged by size:
1135 1022 979 695 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 466 498 611 643 1921 1951 1953
Size: 32 166 32 113 32 1278 30 2
Cuts at: 1953 1983 268
Size: 30 2364
Fragments arranged by size:
2364 1278 166 113 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1118 1118
Size: 4079
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3831
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4079
MluI A'CGCG_T
Cuts at: 3512 3512
Size: 4079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 597 2750 2934 197
Size: 400 2153 184 1342
Fragments arranged by size:
2153 1342 400 184
MscI TGG'CCA
Cuts at: 599 658 1840 599
Size: 59 1182 2838
Fragments arranged by size:
2838 1182 59
MslI CAynn'nnrTG
Cuts at: 366 499 2068 2195 366
Size: 133 1569 127 2250
Fragments arranged by size:
2250 1569 133 127
MspA1I CmG'CkG
Cuts at: 654 1190 1504 1864 2877 3122 654
Size: 536 314 360 1013 245 1611
Fragments arranged by size:
1611 1013 536 360 314 245
NaeI GCC'GGC
Cuts at: 2260 2260
Size: 4079
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3831
NcoI C'CATG_G
Cuts at: 107 107
Size: 4079
NdeI CA'TA_TG
Cuts at: 631 631
Size: 4079
NgoMIV G'CCGG_C
Cuts at: 2258 2258
Size: 4079
NspI r_CATG'y
Cuts at: 1132 1632 2539 1132
Size: 500 907 2672
Fragments arranged by size:
2672 907 500
PciI A'CATG_T
Cuts at: 2535 2535
Size: 4079
PfoI T'CCnGG_A
Cuts at: 125 266 125
Size: 141 3938
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PpuMI rG'GwC_Cy
Cuts at: 258 302 416 513 258
Size: 44 114 97 3824
Fragments arranged by size:
3824 114 97 44
PstI C_TGCA'G
Cuts at: 1126 1811 1126
Size: 685 3394
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 654 1504 1864 654
Size: 850 360 2869
Fragments arranged by size:
2869 850 360
RsrII CG'GwC_CG
Cuts at: 2274 2274
Size: 4079
SacI G_AGCT'C
Cuts at: 1093 1093
Size: 4079
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SanDI GG'GwC_CC
Cuts at: 258 302 258
Size: 44 4035
SapI GCTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4079
ScaI AGT'ACT
Cuts at: 3596 3596
Size: 4079
SexAI A'CCwGG_T
Cuts at: 515 515
Size: 4079
SfcI C'TryA_G
Cuts at: 33 828 1122 1807 2800 2991 33
Size: 795 294 685 993 191 1121
Fragments arranged by size:
1121 993 795 685 294 191
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3831
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4079
SmlI C'TyrA_G
Cuts at: 1234 2149 2641 2903 1234
Size: 915 492 262 2410
Fragments arranged by size:
2410 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
SspI AAT'ATT
Cuts at: 210 210
Size: 4079
StyI C'CwwG_G
Cuts at: 107 849 1213 1567 107
Size: 742 364 354 2619
Fragments arranged by size:
2619 742 364 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 456 924 1011 1662 1795 2254 2463 127
Size: 329 468 87 651 133 459 209 1743
Fragments arranged by size:
1743 651 468 459 329 209 133 87
TatI w'GTAC_w
Cuts at: 3594 3594
Size: 4079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 900 1876 900
Size: 976 3103
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 744 1574 744
Size: 830 3249
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4079
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI ApaI AscI BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI