Restriction map for
pF1KB5856
(Circular) (Six-base) MAPSORT of: pF1KB5856.seq Check: 188 from: 1 to: 4580
pF1KB5856 4580 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1618 1618
Size: 4580
Acc65I G'GTAC_C
Cuts at: 1596 1596
Size: 4580
AclI AA'CG_TT
Cuts at: 4420 4420
Size: 4580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1202 2405 2837 3584 3852 536
Size: 666 1203 432 747 268 1264
Fragments arranged by size:
1264 1203 747 666 432 268
AflIII A'CryG_T
Cuts at: 771 1488 1932 3036 4013 771
Size: 717 444 1104 977 1338
Fragments arranged by size:
1338 1104 977 717 444
AgeI A'CCGG_T
Cuts at: 2968 2968
Size: 4580
AlwNI CAG_nnn'CTG
Cuts at: 1885 3452 1885
Size: 1567 3013
ApaLI G'TGCA_C
Cuts at: 3350 3350
Size: 4580
ApoI r'AATT_y
Cuts at: 315 393 460 735 1393 1567 1584 3933 4189
Size: 78 67 275 658 174 17 2349 256
Cuts at: 4189 315
Size: 706
Fragments arranged by size:
2349 706 658 275 256 174 78 67
17
AseI AT'TA_AT
Cuts at: 20 926 20
Size: 906 3674
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
AvaI C'yCGr_G
Cuts at: 1206 1600 1206
Size: 394 4186
BamHI G'GATC_C
Cuts at: 1605 1605
Size: 4580
BanI G'GyrC_C
Cuts at: 166 1596 2010 2258 2293 166
Size: 1430 414 248 35 2453
Fragments arranged by size:
2453 1430 414 248 35
BanII G_rGCy'C
Cuts at: 1442 1594 2624 1442
Size: 152 1030 3398
Fragments arranged by size:
3398 1030 152
BbeI G_GCGC'C
Cuts at: 2014 2262 2014
Size: 248 4332
BbsI GAAGACnn'nnnn_
Cuts at: 1124 1124
Size: 4580
BciVI GTATCCnnnnn_n'
Cuts at: 735 2478 3239 4580 735
Size: 1743 761 1341 735
Fragments arranged by size:
1743 1341 761 735
BclI T'GATC_A
Cuts at: 372 1323 2100 372
Size: 951 777 2852
Fragments arranged by size:
2852 951 777
BfrBI ATG'CAT
Cuts at: 1110 1110
Size: 4580
BglII A'GATC_T
Cuts at: 2095 4132 2095
Size: 2037 2543
BlpI GC'TnA_GC
Cuts at: 889 1692 889
Size: 803 3777
Bme1580I G_kGCm'C
Cuts at: 169 2205 2298 3354 169
Size: 2036 93 1056 1395
Fragments arranged by size:
2036 1395 1056 93
BmgBI CAC'GTC
Cuts at: 1034 1200 1034
Size: 166 4414
BmrI ACTGGGnnnn_n'
Cuts at: 1792 1873 2208 4352 1792
Size: 81 335 2144 2020
Fragments arranged by size:
2144 2020 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1073 1287 1314 1073
Size: 214 27 4339
Fragments arranged by size:
4339 214 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1756 2635 3127 3425 1756
Size: 879 492 298 2911
Fragments arranged by size:
2911 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4284 4284
Size: 4580
BsaAI yAC'GTr
Cuts at: 1933 1933
Size: 4580
BsaBI GATnn'nnATC
Cuts at: 6 590 2094 4131 6
Size: 584 1504 2037 455
Fragments arranged by size:
2037 1504 584 455
BsaHI Gr'CG_yC
Cuts at: 2011 2259 2961 4457 2011
Size: 248 702 1496 2134
Fragments arranged by size:
2134 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1769 2290 2968 3242 3389 3800 1769
Size: 521 678 274 147 411 2549
Fragments arranged by size:
2549 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 106 532 106
Size: 426 4154
BseYI C'CCAG_C
Cuts at: 2005 2763 3340 3836 2005
Size: 758 577 496 2749
Fragments arranged by size:
2749 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2122 2168 3376 104
Size: 2018 46 1208 1308
Fragments arranged by size:
2018 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1442 1594 2372 2562 3354 1442
Size: 152 778 190 792 2668
Fragments arranged by size:
2668 792 778 190 152
BsmBI CGTCTCn'nnnn_
Cuts at: 563 563
Size: 4580
Bsp1286I G_dGCh'C
Cuts at: 169 1442 1594 2205 2298 2372 2562 2624 3354
Size: 1273 152 611 93 74 190 62 730
Cuts at: 3354 169
Size: 1395
Fragments arranged by size:
1395 1273 730 611 190 152 93 74
62
BspEI T'CCGG_A
Cuts at: 3800 3800
Size: 4580
BspHI T'CATG_A
Cuts at: 530 3756 530
Size: 3226 1354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1630 1844 2146 2527 1630
Size: 214 302 381 3683
Fragments arranged by size:
3683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2872 2926 4410 4575 50
Size: 2822 54 1484 165 55
Fragments arranged by size:
2822 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2492 2492
Size: 4580
BsrFI r'CCGG_y
Cuts at: 2578 2759 2968 2578
Size: 181 209 4190
Fragments arranged by size:
4190 209 181
BsrGI T'GTAC_A
Cuts at: 620 620
Size: 4580
BssSI C'ACGA_G
Cuts at: 2851 3209 2851
Size: 358 4222
BstAPI GCAn_nnn'nTGC
Cuts at: 1957 1957
Size: 4580
BstBI TT'CG_AA
Cuts at: 2941 2941
Size: 4580
BstXI CCAn_nnnn'nTGG
Cuts at: 1362 1362
Size: 4580
BstYI r'GATC_y
Cuts at: 1 174 653 1500 1605 2072 2095 2430 2676
Size: 173 479 847 105 467 23 335 246
Cuts at: 2676 3688 3774 3786 4132 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 847 479 467 449 346 335 246
173 105 86 23 12
BtgI C'CryG_G
Cuts at: 107 170 107
Size: 63 4517
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1266 2530 2711 2874 1266
Size: 1264 181 163 2972
Fragments arranged by size:
2972 1264 181 163
BtsI GCAGTG_nn'
Cuts at: 1823 1823
Size: 4580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1452 1487 1452
Size: 35 4545
DraI TTT'AAA
Cuts at: 1289 1579 1289
Size: 290 4290
DrdI GACnn_nn'nnGTC
Cuts at: 2286 3144 2286
Size: 858 3722
EaeI y'GGCC_r
Cuts at: 2165 2339 2730 2757 4102 2165
Size: 174 391 27 1345 2643
Fragments arranged by size:
2643 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2165 2165
Size: 4580
EarI CTCTTCn'nnn_
Cuts at: 1070 2603 2813 1070
Size: 1533 210 2837
Fragments arranged by size:
2837 1533 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2788 3098 3244 4390 2788
Size: 310 146 1146 2978
Fragments arranged by size:
2978 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1440 1592 1440
Size: 152 4428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1073 1202 1287 1314 2405 2837 3584 3852
Size: 537 129 85 27 1091 432 747 268
Cuts at: 3852 536
Size: 1264
Fragments arranged by size:
1264 1091 747 537 432 268 129 85
27
EcoO109I rG'GnC_Cy
Cuts at: 273 1719 273
Size: 1446 3134
EcoRI G'AATT_C
Cuts at: 315 735 1584 315
Size: 420 849 3311
Fragments arranged by size:
3311 849 420
EcoRV GAT'ATC
Cuts at: 507 507
Size: 4580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 862 1007 2599 862
Size: 145 1592 2843
Fragments arranged by size:
2843 1592 145
FspI TGC'GCA
Cuts at: 2361 2361
Size: 4580
HaeII r_GCGC'y
Cuts at: 2014 2262 3284 4263 2014
Size: 248 1022 979 2331
Fragments arranged by size:
2331 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1069 1101 1424 1456 2422 2452 2454 2484 1069
Size: 32 323 32 966 30 2 30 3165
Fragments arranged by size:
3165 966 323 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1619 1619
Size: 4580
KasI G'GCGC_C
Cuts at: 2010 2258 2010
Size: 248 4332
KpnI G_GTAC'C
Cuts at: 1600 1600
Size: 4580
MfeI C'AATT_G
Cuts at: 215 215
Size: 4580
MluI A'CGCG_T
Cuts at: 4013 4013
Size: 4580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 405 3251 3435 405
Size: 2846 184 1550
Fragments arranged by size:
2846 1550 184
MscI TGG'CCA
Cuts at: 2341 2341
Size: 4580
MslI CAynn'nnrTG
Cuts at: 380 616 2569 2696 380
Size: 236 1953 127 2264
Fragments arranged by size:
2264 1953 236 127
MspA1I CmG'CkG
Cuts at: 172 1358 1691 2005 2365 3378 3623 172
Size: 1186 333 314 360 1013 245 1129
Fragments arranged by size:
1186 1129 1013 360 333 314 245
NaeI GCC'GGC
Cuts at: 2761 2761
Size: 4580
NarI GG'CG_CC
Cuts at: 2011 2259 2011
Size: 248 4332
NcoI C'CATG_G
Cuts at: 107 107
Size: 4580
NgoMIV G'CCGG_C
Cuts at: 2759 2759
Size: 4580
NsiI A_TGCA'T
Cuts at: 1112 1112
Size: 4580
NspI r_CATG'y
Cuts at: 775 1110 1492 1633 2133 3040 775
Size: 335 382 141 500 907 2315
Fragments arranged by size:
2315 907 500 382 335 141
PciI A'CATG_T
Cuts at: 771 1488 3036 771
Size: 717 1548 2315
Fragments arranged by size:
2315 1548 717
PfoI T'CCnGG_A
Cuts at: 1125 1125
Size: 4580
PmeI GTTT'AAAC
Cuts at: 1579 1579
Size: 4580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1224 1256 3719 3751 1224
Size: 32 2463 32 2053
Fragments arranged by size:
2463 2053 32 32
PpuMI rG'GwC_Cy
Cuts at: 273 273
Size: 4580
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 825 857 825
Size: 32 4548
PstI C_TGCA'G
Cuts at: 1627 2312 1627
Size: 685 3895
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4580
PvuII CAG'CTG
Cuts at: 1358 2005 2365 1358
Size: 647 360 3573
Fragments arranged by size:
3573 647 360
RsrII CG'GwC_CG
Cuts at: 2775 2775
Size: 4580
SacI G_AGCT'C
Cuts at: 1442 1594 1442
Size: 152 4428
SacII CC_GC'GG
Cuts at: 173 173
Size: 4580
SalI G'TCGA_C
Cuts at: 1617 1617
Size: 4580
SapI GCTCTTCn'nnn_
Cuts at: 1070 2603 2813 1070
Size: 1533 210 2837
Fragments arranged by size:
2837 1533 210
SbfI CC_TGCA'GG
Cuts at: 1627 1627
Size: 4580
ScaI AGT'ACT
Cuts at: 745 4097 745
Size: 3352 1228
SfcI C'TryA_G
Cuts at: 33 1623 2308 3301 3492 33
Size: 1590 685 993 191 1121
Fragments arranged by size:
1590 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2012 2260 2012
Size: 248 4332
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
SmaI CCC'GGG
Cuts at: 1208 1602 1208
Size: 394 4186
SmlI C'TyrA_G
Cuts at: 1735 2650 3142 3404 1735
Size: 915 492 262 2911
Fragments arranged by size:
2911 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4580
SphI G_CATG'C
Cuts at: 1110 1633 2133 1110
Size: 523 500 3557
Fragments arranged by size:
3557 523 500
SspI AAT'ATT
Cuts at: 345 366 953 1191 345
Size: 21 587 238 3734
Fragments arranged by size:
3734 587 238 21
StyI C'CwwG_G
Cuts at: 107 1714 2068 107
Size: 1607 354 2619
Fragments arranged by size:
2619 1607 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2163 2296 2755 2964 2163
Size: 133 459 209 3779
Fragments arranged by size:
3779 459 209 133
TatI w'GTAC_w
Cuts at: 620 743 4095 620
Size: 123 3352 1105
Fragments arranged by size:
3352 1105 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4097 4129 4097
Size: 32 4548
Tth111I GACn'n_nGTC
Cuts at: 1201 2377 1201
Size: 1176 3404
XbaI T'CTAG_A
Cuts at: 1611 1611
Size: 4580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1270 2075 1270
Size: 805 3775
XmaI C'CCGG_G
Cuts at: 1206 1600 1206
Size: 394 4186
XmnI GAAnn'nnTTC
Cuts at: 1190 1311 1190
Size: 121 4459
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI FseI FspAI HindIII HpaI
NdeI NheI NotI NruI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI