Restriction map for
pF1KB5861
(Circular) (Six-base) MAPSORT of: pF1KB5861.seq Check: 7465 from: 1 to: 4496
pF1KB5861 4496 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 266 1512 266
Size: 1246 3250
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1421 2321 2753 3500 3768 1421
Size: 900 432 747 268 2149
Fragments arranged by size:
2149 900 747 432 268
AfeI AGC'GCT
Cuts at: 640 640
Size: 4496
AflII C'TTAA_G
Cuts at: 652 652
Size: 4496
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 263 2884 263
Size: 2621 1875
AhdI GACnn_n'nnGTC
Cuts at: 236 236
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 900 1488 1801 3368 900
Size: 588 313 1567 2028
Fragments arranged by size:
2028 1567 588 313
ApaI G_GGCC'C
Cuts at: 125 125
Size: 4496
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 1500 3849 4105 1500
Size: 2349 256 1891
Fragments arranged by size:
2349 1891 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 125 593 1175 1453 1516 125
Size: 468 582 278 63 3105
Fragments arranged by size:
3105 582 468 278 63
BamHI G'GATC_C
Cuts at: 477 1521 477
Size: 1044 3452
BanI G'GyrC_C
Cuts at: 204 266 743 1476 1512 1926 2174 2209 204
Size: 62 477 733 36 414 248 35 2491
Fragments arranged by size:
2491 733 477 414 248 62 36 35
BanII G_rGCy'C
Cuts at: 125 416 1510 2540 125
Size: 291 1094 1030 2081
Fragments arranged by size:
2081 1094 1030 291
BbeI G_GCGC'C
Cuts at: 208 1930 2178 208
Size: 1722 248 2526
Fragments arranged by size:
2526 1722 248
BbvCI CC'TCA_GC
Cuts at: 1147 1210 1147
Size: 63 4433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4462
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglI GCCn_nnn'nGGC
Cuts at: 181 1180 181
Size: 999 3497
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 361 1608 361
Size: 1247 3249
Bme1580I G_kGCm'C
Cuts at: 125 1481 2121 2214 3270 125
Size: 1356 640 93 1056 1351
Fragments arranged by size:
1356 1351 1056 640 93
BmgBI CAC'GTC
Cuts at: 393 393
Size: 4496
BmrI ACTGGGnnnn_n'
Cuts at: 600 697 780 1708 1789 2124 4268 600
Size: 97 83 928 81 335 2144 828
Fragments arranged by size:
2144 928 828 335 97 83 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 151 2863 151
Size: 2712 1784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 256 313 589 700 922 946 1069 1273
Size: 42 57 276 111 222 24 123 204
Cuts at: 1273 1391 214
Size: 118 3319
Fragments arranged by size:
3319 276 222 204 123 118 111 57
42 24
Bpu10I CC'TnA_GC
Cuts at: 1147 1210 1147
Size: 63 4433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 436 1351 1672 2551 3043 3341 400
Size: 36 915 321 879 492 298 1555
Fragments arranged by size:
1555 915 879 492 321 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 214 4200 214
Size: 3986 510
BsaAI yAC'GTr
Cuts at: 1849 1849
Size: 4496
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 205 467 1927 2175 2877 4373 205
Size: 262 1460 248 702 1496 328
Fragments arranged by size:
1496 1460 702 328 262 248
BsaWI w'CCGG_w
Cuts at: 263 1685 2206 2884 3158 3305 3716 263
Size: 1422 521 678 274 147 411 1043
Fragments arranged by size:
1422 1043 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 919 949 919
Size: 30 4466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 709 820 967 991 994 1066 1177 232
Size: 477 111 147 24 3 72 111 3551
Fragments arranged by size:
3551 477 147 111 111 72 24 3
BseYI C'CCAG_C
Cuts at: 1103 1202 1401 1481 1921 2679 3256 3752 1103
Size: 99 199 80 440 758 577 496 1847
Fragments arranged by size:
1847 758 577 496 440 199 99 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1273
Size: 4496
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 416 755 1510 2288 2478 3270 416
Size: 339 755 778 190 792 1642
Fragments arranged by size:
1642 792 778 755 339 190
BsmI GAATG_Cn'
Cuts at: 1197 1197
Size: 4496
BsmBI CGTCTCn'nnnn_
Cuts at: 289 660 289
Size: 371 4125
Bsp1286I G_dGCh'C
Cuts at: 125 416 755 1481 1510 2121 2214 2288 2478
Size: 291 339 726 29 611 93 74 190
Cuts at: 2478 2540 3270 125
Size: 62 730 1351
Fragments arranged by size:
1351 730 726 611 339 291 190 93
74 62 29
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 624 3672 624
Size: 3048 1448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 1760 2062 2443 1546
Size: 214 302 381 3599
Fragments arranged by size:
3599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 353 1458 2788 2842 4326 4491 50
Size: 303 1105 1330 54 1484 165 55
Fragments arranged by size:
1484 1330 1105 303 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 263 740 1411 2494 2675 2884 263
Size: 477 671 1083 181 209 1875
Fragments arranged by size:
1875 1083 671 477 209 181
BsrGI T'GTAC_A
Cuts at: 486 486
Size: 4496
BssSI C'ACGA_G
Cuts at: 661 1039 2767 3125 661
Size: 378 1728 358 2032
Fragments arranged by size:
2032 1728 378 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1284 1873 1284
Size: 589 3907
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstEII G'GTnAC_C
Cuts at: 1414 1414
Size: 4496
BstXI CCAn_nnnn'nTGG
Cuts at: 924 924
Size: 4496
BstYI r'GATC_y
Cuts at: 1 477 1521 1988 2011 2346 2592 3604 3690
Size: 476 1044 467 23 335 246 1012 86
Cuts at: 3690 3702 4048 1
Size: 12 346 449
Fragments arranged by size:
1044 1012 476 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1355 1466 1355
Size: 111 4385
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 1739 1739
Size: 4496
ClaI AT'CG_AT
Cuts at: 284 284
Size: 4496
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 587 2081 2255 2646 2673 4018 587
Size: 1494 174 391 27 1345 1065
Fragments arranged by size:
1494 1345 1065 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 623 722 916 1136 1154 1258 2519 2729 623
Size: 99 194 220 18 104 1261 210 2390
Fragments arranged by size:
2390 1261 220 210 194 104 99 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 727 2704 3014 3160 4306 727
Size: 1977 310 146 1146 917
Fragments arranged by size:
1977 1146 917 310 146
EcoICRI GAG'CTC
Cuts at: 414 1508 414
Size: 1094 3402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 256 313 589 700 922 946 1069 1273
Size: 42 57 276 111 222 24 123 204
Cuts at: 1273 1391 1421 2321 2753 3500 3768 214
Size: 118 30 900 432 747 268 942
Fragments arranged by size:
942 900 747 432 276 268 222 204
123 118 111 57 42 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 274 1213 274
Size: 939 3557
EcoO109I rG'GnC_Cy
Cuts at: 122 597 1338 1635 122
Size: 475 741 297 2983
Fragments arranged by size:
2983 741 475 297
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
EcoRV GAT'ATC
Cuts at: 867 867
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 867 2515 867
Size: 1648 2848
FspI TGC'GCA
Cuts at: 991 2277 991
Size: 1286 3210
HaeII r_GCGC'y
Cuts at: 208 642 741 1155 1209 1930 2178 3200 4179
Size: 434 99 414 54 721 248 1022 979
Cuts at: 4179 208
Size: 525
Fragments arranged by size:
1022 979 721 525 434 414 248 99
54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 222 254 1212 1244 2338 2368 2370 2400 222
Size: 32 958 32 1094 30 2 30 2318
Fragments arranged by size:
2318 1094 958 32 32 30 30 2
HincII GTy'rAC
Cuts at: 765 1535 765
Size: 770 3726
KasI G'GCGC_C
Cuts at: 204 1926 2174 204
Size: 1722 248 2526
Fragments arranged by size:
2526 1722 248
KpnI G_GTAC'C
Cuts at: 270 1516 270
Size: 1246 3250
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 2485 2612 2485
Size: 127 4369
MspA1I CmG'CkG
Cuts at: 150 1302 1607 1921 2281 3294 3539 150
Size: 1152 305 314 360 1013 245 1107
Fragments arranged by size:
1152 1107 1013 360 314 305 245
NaeI GCC'GGC
Cuts at: 742 2677 742
Size: 1935 2561
NarI GG'CG_CC
Cuts at: 205 1927 2175 205
Size: 1722 248 2526
Fragments arranged by size:
2526 1722 248
NgoMIV G'CCGG_C
Cuts at: 740 2675 740
Size: 1935 2561
NspI r_CATG'y
Cuts at: 966 996 1006 1549 2049 2956 966
Size: 30 10 543 500 907 2506
Fragments arranged by size:
2506 907 543 500 30 10
PasI CC'CwG_GG
Cuts at: 1242 1242
Size: 4496
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 1488 1488
Size: 4496
PfoI T'CCnGG_A
Cuts at: 272 884 272
Size: 612 3884
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 597 597
Size: 4496
PspOMI G'GGCC_C
Cuts at: 121 121
Size: 4496
PstI C_TGCA'G
Cuts at: 326 1277 1543 2228 326
Size: 951 266 685 2594
Fragments arranged by size:
2594 951 685 266
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1921 2281 1921
Size: 360 4136
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 416 1510 416
Size: 1094 3402
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 722 1136 2519 2729 722
Size: 414 1383 210 2489
Fragments arranged by size:
2489 1383 414 210
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 322 1273 1539 2224 3217 3408 33
Size: 289 951 266 685 993 191 1121
Fragments arranged by size:
1121 993 951 685 289 266 191
SfoI GGC'GCC
Cuts at: 206 1928 2176 206
Size: 1722 248 2526
Fragments arranged by size:
2526 1722 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 595 1177 1518 595
Size: 582 341 3573
Fragments arranged by size:
3573 582 341
SmlI C'TyrA_G
Cuts at: 415 451 652 1366 1453 1651 2566 3058 3320
Size: 36 201 714 87 198 915 492 262
Cuts at: 3320 415
Size: 1591
Fragments arranged by size:
1591 915 714 492 262 201 198 87
36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 996 1549 2049 996
Size: 553 500 3443
Fragments arranged by size:
3443 553 500
StuI AGG'CCT
Cuts at: 251 251
Size: 4496
StyI C'CwwG_G
Cuts at: 917 1196 1630 1984 917
Size: 279 434 354 3429
Fragments arranged by size:
3429 434 354 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1391 2079 2212 2671 2880 1391
Size: 688 133 459 209 3007
Fragments arranged by size:
3007 688 459 209 133
TatI w'GTAC_w
Cuts at: 254 288 486 4011 254
Size: 34 198 3525 739
Fragments arranged by size:
3525 739 198 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4464
Tth111I GACn'n_nGTC
Cuts at: 379 2293 379
Size: 1914 2582
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 1991 489
Size: 1502 2994
XhoI C'TCGA_G
Cuts at: 1453 1453
Size: 4496
XmaI C'CCGG_G
Cuts at: 593 1175 1516 593
Size: 582 341 3573
Fragments arranged by size:
3573 582 341
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AleI AlfI AloI AscI AvrII BaeI
BbsI BfrBI BmtI BsiWI BssHII BstZ17I BtgI CspCI
DraIII FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI