(Circular) (Six-base) MAPSORT of: pF1KB5865.seq Check: 8651 from: 1 to: 4496 pF1KB5865 4496 bp With 182 enzymes: SgfI * October 7, 2009 14:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1336 1336 Size: 4496 AccI GT'mk_AC Cuts at: 1534 1534 Size: 4496 Acc65I G'GTAC_C Cuts at: 1512 1512 Size: 4496 AclI AA'CG_TT Cuts at: 4336 4336 Size: 4496 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 232 328 376 628 1051 1498 2321 2753 3500 Size: 96 48 252 423 447 823 432 747 Cuts at: 3500 3768 232 Size: 268 960 Fragments arranged by size: 960 823 747 447 432 423 268 252 96 48 AflIII A'CryG_T Cuts at: 1848 2952 3929 1848 Size: 1104 977 2415 Fragments arranged by size: 2415 1104 977 AgeI A'CCGG_T Cuts at: 2884 2884 Size: 4496 AleI CACnn'nnGTG Cuts at: 1084 1084 Size: 4496 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1102 1102 Size: 4496 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1123 1155 1123 Size: 32 4464 AlwNI CAG_nnn'CTG Cuts at: 123 210 771 924 1029 1053 1308 1401 1452 Size: 87 561 153 105 24 255 93 51 Cuts at: 1452 1801 3368 123 Size: 349 1567 1251 Fragments arranged by size: 1567 1251 561 349 255 153 105 93 87 51 24 ApaLI G'TGCA_C Cuts at: 506 3266 506 Size: 2760 1736 ApoI r'AATT_y Cuts at: 517 1500 3849 4105 517 Size: 983 2349 256 908 Fragments arranged by size: 2349 983 908 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4496 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4496 AvaI C'yCGr_G Cuts at: 1036 1273 1516 1036 Size: 237 243 4016 Fragments arranged by size: 4016 243 237 BamHI G'GATC_C Cuts at: 370 1521 370 Size: 1151 3345 BanI G'GyrC_C Cuts at: 1512 1926 2174 2209 1512 Size: 414 248 35 3799 Fragments arranged by size: 3799 414 248 35 BanII G_rGCy'C Cuts at: 827 915 1462 1510 2540 827 Size: 88 547 48 1030 2783 Fragments arranged by size: 2783 1030 547 88 48 BbeI G_GCGC'C Cuts at: 1930 2178 1930 Size: 248 4248 BbsI GAAGACnn'nnnn_ Cuts at: 560 781 560 Size: 221 4275 BbvCI CC'TCA_GC Cuts at: 572 572 Size: 4496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1180 1214 1264 1298 1180 Size: 34 50 34 4378 Fragments arranged by size: 4378 50 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2394 3155 4496 2394 Size: 761 1341 2394 Fragments arranged by size: 2394 1341 761 BclI T'GATC_A Cuts at: 671 800 2016 671 Size: 129 1216 3151 Fragments arranged by size: 3151 1216 129 BglII A'GATC_T Cuts at: 2011 4048 2011 Size: 2037 2459 BlpI GC'TnA_GC Cuts at: 1157 1608 1157 Size: 451 4045 Bme1580I G_kGCm'C Cuts at: 510 1081 2121 2214 3270 510 Size: 571 1040 93 1056 1736 Fragments arranged by size: 1736 1056 1040 571 93 BmrI ACTGGGnnnn_n' Cuts at: 407 1318 1708 1789 2124 4268 407 Size: 911 390 81 335 2144 635 Fragments arranged by size: 2144 911 635 390 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2863 2863 Size: 4496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 328 681 1432 328 Size: 353 751 3392 Fragments arranged by size: 3392 751 353 Bpu10I CC'TnA_GC Cuts at: 572 572 Size: 4496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 484 1672 2551 3043 3341 484 Size: 1188 879 492 298 1639 Fragments arranged by size: 1639 1188 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4200 4200 Size: 4496 BsaAI yAC'GTr Cuts at: 378 1849 378 Size: 1471 3025 BsaBI GATnn'nnATC Cuts at: 6 2010 4047 6 Size: 2004 2037 455 Fragments arranged by size: 2037 2004 455 BsaHI Gr'CG_yC Cuts at: 1313 1927 2175 2877 4373 1313 Size: 614 248 702 1496 1436 Fragments arranged by size: 1496 1436 702 614 248 BsaWI w'CCGG_w Cuts at: 1685 2206 2884 3158 3305 3716 1685 Size: 521 678 274 147 411 2465 Fragments arranged by size: 2465 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1123 1153 1123 Size: 30 4466 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 134 298 301 1162 1165 134 Size: 164 3 861 3 3465 Fragments arranged by size: 3465 861 164 3 3 BseYI C'CCAG_C Cuts at: 855 1016 1355 1455 1921 2679 3256 3752 855 Size: 161 339 100 466 758 577 496 1599 Fragments arranged by size: 1599 758 577 496 466 339 161 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1087 1087 Size: 4496 BsiEI CG_ry'CG Cuts at: 104 2038 2084 3292 104 Size: 1934 46 1208 1308 Fragments arranged by size: 1934 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 510 991 1091 1510 2288 2478 3270 510 Size: 481 100 419 778 190 792 1736 Fragments arranged by size: 1736 792 778 481 419 190 100 BsiWI C'GTAC_G Cuts at: 378 378 Size: 4496 Bsp1286I G_dGCh'C Cuts at: 510 827 915 991 1081 1091 1462 1510 2121 Size: 317 88 76 90 10 371 48 611 Cuts at: 2121 2214 2288 2478 2540 3270 510 Size: 93 74 190 62 730 1736 Fragments arranged by size: 1736 730 611 371 317 190 93 90 88 76 74 62 48 10 BspEI T'CCGG_A Cuts at: 3716 3716 Size: 4496 BspHI T'CATG_A Cuts at: 3672 3672 Size: 4496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 779 1038 1336 1546 1760 2062 2443 779 Size: 259 298 210 214 302 381 2832 Fragments arranged by size: 2832 381 302 298 259 214 210 BsrBI CCG'CTC Cuts at: 50 954 2788 2842 4326 4491 50 Size: 904 1834 54 1484 165 55 Fragments arranged by size: 1834 1484 904 165 55 54 BsrDI GCAATG_nn' Cuts at: 2408 2408 Size: 4496 BsrFI r'CCGG_y Cuts at: 2494 2675 2884 2494 Size: 181 209 4106 Fragments arranged by size: 4106 209 181 BssSI C'ACGA_G Cuts at: 829 2767 3125 829 Size: 1938 358 2200 Fragments arranged by size: 2200 1938 358 BstAPI GCAn_nnn'nTGC Cuts at: 771 924 1873 771 Size: 153 949 3394 Fragments arranged by size: 3394 949 153 BstBI TT'CG_AA Cuts at: 2857 2857 Size: 4496 BstXI CCAn_nnnn'nTGG Cuts at: 1226 1226 Size: 4496 BstYI r'GATC_y Cuts at: 1 370 495 1521 1988 2011 2346 2592 3604 Size: 369 125 1026 467 23 335 246 1012 Cuts at: 3604 3690 3702 4048 1 Size: 86 12 346 449 Fragments arranged by size: 1026 1012 467 449 369 346 335 246 125 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1054 1054 Size: 4496 BtgI C'CryG_G Cuts at: 107 941 107 Size: 834 3662 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2446 2627 2790 2446 Size: 181 163 4152 Fragments arranged by size: 4152 181 163 BtsI GCAGTG_nn' Cuts at: 956 1108 1739 956 Size: 152 631 3713 Fragments arranged by size: 3713 631 152 DraI TTT'AAA Cuts at: 1495 1495 Size: 4496 DrdI GACnn_nn'nnGTC Cuts at: 2202 3060 2202 Size: 858 3638 EaeI y'GGCC_r Cuts at: 173 938 1373 1403 2081 2255 2646 2673 4018 Size: 765 435 30 678 174 391 27 1345 Cuts at: 4018 173 Size: 651 Fragments arranged by size: 1345 765 678 651 435 391 174 30 27 EagI C'GGCC_G Cuts at: 2081 2081 Size: 4496 EarI CTCTTCn'nnn_ Cuts at: 235 592 701 2519 2729 235 Size: 357 109 1818 210 2002 Fragments arranged by size: 2002 1818 357 210 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 580 2704 3014 3160 4306 580 Size: 2124 310 146 1146 770 Fragments arranged by size: 2124 1146 770 310 146 EcoICRI GAG'CTC Cuts at: 1508 1508 Size: 4496 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 232 328 376 628 681 1051 1432 1498 2321 Size: 96 48 252 53 370 381 66 823 Cuts at: 2321 2753 3500 3768 232 Size: 432 747 268 960 Fragments arranged by size: 960 823 747 432 381 370 268 252 96 66 53 48 EcoNI CCTnn'n_nnAGG Cuts at: 832 1066 832 Size: 234 4262 EcoO109I rG'GnC_Cy Cuts at: 339 768 972 1050 1058 1350 1635 339 Size: 429 204 78 8 292 285 3200 Fragments arranged by size: 3200 429 292 285 204 78 8 EcoRI G'AATT_C Cuts at: 1500 1500 Size: 4496 EcoRV GAT'ATC Cuts at: 468 468 Size: 4496 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 603 633 2515 603 Size: 30 1882 2584 Fragments arranged by size: 2584 1882 30 FspI TGC'GCA Cuts at: 764 2277 764 Size: 1513 2983 HaeII r_GCGC'y Cuts at: 863 1270 1930 2178 3200 4179 863 Size: 407 660 248 1022 979 1180 Fragments arranged by size: 1180 1022 979 660 407 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 129 161 2338 2368 2370 2400 129 Size: 32 2177 30 2 30 2225 Fragments arranged by size: 2225 2177 32 30 30 2 HincII GTy'rAC Cuts at: 1467 1535 1467 Size: 68 4428 KasI G'GCGC_C Cuts at: 1926 2174 1926 Size: 248 4248 KpnI G_GTAC'C Cuts at: 1516 1516 Size: 4496 MluI A'CGCG_T Cuts at: 3929 3929 Size: 4496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3167 3351 3167 Size: 184 4312 MscI TGG'CCA Cuts at: 940 1375 1405 2257 940 Size: 435 30 852 3179 Fragments arranged by size: 3179 852 435 30 MslI CAynn'nnrTG Cuts at: 1084 2485 2612 1084 Size: 1401 127 2968 Fragments arranged by size: 2968 1401 127 MspA1I CmG'CkG Cuts at: 1020 1607 1921 2281 3294 3539 1020 Size: 587 314 360 1013 245 1977 Fragments arranged by size: 1977 1013 587 360 314 245 NaeI GCC'GGC Cuts at: 2677 2677 Size: 4496 NarI GG'CG_CC Cuts at: 1927 2175 1927 Size: 248 4248 NcoI C'CATG_G Cuts at: 107 107 Size: 4496 NgoMIV G'CCGG_C Cuts at: 2675 2675 Size: 4496 NspI r_CATG'y Cuts at: 156 654 1549 2049 2956 156 Size: 498 895 500 907 1696 Fragments arranged by size: 1696 907 895 500 498 PciI A'CATG_T Cuts at: 2952 2952 Size: 4496 PflMI CCAn_nnn'nTGG Cuts at: 753 1263 1401 753 Size: 510 138 3848 Fragments arranged by size: 3848 510 138 PfoI T'CCnGG_A Cuts at: 305 1445 305 Size: 1140 3356 PmeI GTTT'AAAC Cuts at: 1495 1495 Size: 4496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 418 450 1123 1155 3635 3667 418 Size: 32 673 32 2480 32 1247 Fragments arranged by size: 2480 1247 673 32 32 32 PpuMI rG'GwC_Cy Cuts at: 768 972 1050 1058 1350 768 Size: 204 78 8 292 3914 Fragments arranged by size: 3914 292 204 78 8 PstI C_TGCA'G Cuts at: 369 920 1049 1193 1304 1385 1421 1543 2228 Size: 551 129 144 111 81 36 122 685 Cuts at: 2228 369 Size: 2637 Fragments arranged by size: 2637 685 551 144 129 122 111 81 36 PvuI CG_AT'CG Cuts at: 104 104 Size: 4496 PvuII CAG'CTG Cuts at: 1921 2281 1921 Size: 360 4136 RsrII CG'GwC_CG Cuts at: 2691 2691 Size: 4496 SacI G_AGCT'C Cuts at: 1510 1510 Size: 4496 SalI G'TCGA_C Cuts at: 1533 1533 Size: 4496 SapI GCTCTTCn'nnn_ Cuts at: 235 592 2519 2729 235 Size: 357 1927 210 2002 Fragments arranged by size: 2002 1927 357 210 SbfI CC_TGCA'GG Cuts at: 369 1385 1543 369 Size: 1016 158 3322 Fragments arranged by size: 3322 1016 158 ScaI AGT'ACT Cuts at: 4013 4013 Size: 4496 SexAI A'CCwGG_T Cuts at: 1121 1394 1121 Size: 273 4223 SfcI C'TryA_G Cuts at: 33 365 916 1045 1189 1300 1381 1417 1539 Size: 332 551 129 144 111 81 36 122 Cuts at: 1539 2224 3217 3408 33 Size: 685 993 191 1121 Fragments arranged by size: 1121 993 685 551 332 191 144 129 122 111 81 36 SfoI GGC'GCC Cuts at: 1928 2176 1928 Size: 248 4248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4496 SmaI CCC'GGG Cuts at: 1518 1518 Size: 4496 SmlI C'TyrA_G Cuts at: 499 1651 2566 3058 3320 499 Size: 1152 915 492 262 1675 Fragments arranged by size: 1675 1152 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4496 SphI G_CATG'C Cuts at: 156 1549 2049 156 Size: 1393 500 2603 Fragments arranged by size: 2603 1393 500 SspI AAT'ATT Cuts at: 739 739 Size: 4496 StuI AGG'CCT Cuts at: 1223 1223 Size: 4496 StyI C'CwwG_G Cuts at: 107 881 1630 1984 107 Size: 774 749 354 2619 Fragments arranged by size: 2619 774 749 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2079 2212 2671 2880 2079 Size: 133 459 209 3695 Fragments arranged by size: 3695 459 209 133 TatI w'GTAC_w Cuts at: 4011 4011 Size: 4496 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1298 1330 4013 4045 1298 Size: 32 2683 32 1749 Fragments arranged by size: 2683 1749 32 32 Tth111I GACn'n_nGTC Cuts at: 1123 2293 1123 Size: 1170 3326 XbaI T'CTAG_A Cuts at: 1527 1527 Size: 4496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 699 888 1991 699 Size: 189 1103 3204 Fragments arranged by size: 3204 1103 189 XmaI C'CCGG_G Cuts at: 1516 1516 Size: 4496 XmnI GAAnn'nnTTC Cuts at: 1138 1138 Size: 4496 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI ApaI AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI