Restriction map for pF1KB5865
 (Circular) (Six-base) MAPSORT of: pF1KB5865.seq  Check: 8651  from: 1  to: 4496



pF1KB5865  4496 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1336    1336 
   Size:       4496
 
AccI GT'mk_AC

Cuts at:   1534    1534 
   Size:       4496
 
Acc65I G'GTAC_C

Cuts at:   1512    1512 
   Size:       4496
 
AclI AA'CG_TT

Cuts at:   4336    4336 
   Size:       4496
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    232     328     376     628    1051    1498    2321    2753    3500 
   Size:         96      48     252     423     447     823     432     747

Cuts at:   3500    3768     232 
   Size:        268     960

  Fragments arranged by size: 

                 960     823     747     447     432     423     268     252
                  96      48
 
AflIII A'CryG_T

Cuts at:   1848    2952    3929    1848 
   Size:       1104     977    2415

  Fragments arranged by size: 

                2415    1104     977
 
AgeI A'CCGG_T

Cuts at:   2884    2884 
   Size:       4496
 
AleI CACnn'nnGTG

Cuts at:   1084    1084 
   Size:       4496
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1102    1102 
   Size:       4496
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1123    1155    1123 
   Size:         32    4464
 
AlwNI CAG_nnn'CTG

Cuts at:    123     210     771     924    1029    1053    1308    1401    1452 
   Size:         87     561     153     105      24     255      93      51

Cuts at:   1452    1801    3368     123 
   Size:        349    1567    1251

  Fragments arranged by size: 

                1567    1251     561     349     255     153     105      93
                  87      51      24
 
ApaLI G'TGCA_C

Cuts at:    506    3266     506 
   Size:       2760    1736
 
ApoI r'AATT_y

Cuts at:    517    1500    3849    4105     517 
   Size:        983    2349     256     908

  Fragments arranged by size: 

                2349     983     908     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4496
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4496
 
AvaI C'yCGr_G

Cuts at:   1036    1273    1516    1036 
   Size:        237     243    4016

  Fragments arranged by size: 

                4016     243     237
 
BamHI G'GATC_C

Cuts at:    370    1521     370 
   Size:       1151    3345
 
BanI G'GyrC_C

Cuts at:   1512    1926    2174    2209    1512 
   Size:        414     248      35    3799

  Fragments arranged by size: 

                3799     414     248      35
 
BanII G_rGCy'C

Cuts at:    827     915    1462    1510    2540     827 
   Size:         88     547      48    1030    2783

  Fragments arranged by size: 

                2783    1030     547      88      48
 
BbeI G_GCGC'C

Cuts at:   1930    2178    1930 
   Size:        248    4248
 
BbsI GAAGACnn'nnnn_

Cuts at:    560     781     560 
   Size:        221    4275
 
BbvCI CC'TCA_GC

Cuts at:    572     572 
   Size:       4496
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1180    1214    1264    1298    1180 
   Size:         34      50      34    4378

  Fragments arranged by size: 

                4378      50      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2394    3155    4496    2394 
   Size:        761    1341    2394

  Fragments arranged by size: 

                2394    1341     761
 
BclI T'GATC_A

Cuts at:    671     800    2016     671 
   Size:        129    1216    3151

  Fragments arranged by size: 

                3151    1216     129
 
BglII A'GATC_T

Cuts at:   2011    4048    2011 
   Size:       2037    2459
 
BlpI GC'TnA_GC

Cuts at:   1157    1608    1157 
   Size:        451    4045
 
Bme1580I G_kGCm'C

Cuts at:    510    1081    2121    2214    3270     510 
   Size:        571    1040      93    1056    1736

  Fragments arranged by size: 

                1736    1056    1040     571      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    407    1318    1708    1789    2124    4268     407 
   Size:        911     390      81     335    2144     635

  Fragments arranged by size: 

                2144     911     635     390     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2863    2863 
   Size:       4496
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    328     681    1432     328 
   Size:        353     751    3392

  Fragments arranged by size: 

                3392     751     353
 
Bpu10I CC'TnA_GC

Cuts at:    572     572 
   Size:       4496
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    484    1672    2551    3043    3341     484 
   Size:       1188     879     492     298    1639

  Fragments arranged by size: 

                1639    1188     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4200    4200 
   Size:       4496
 
BsaAI yAC'GTr

Cuts at:    378    1849     378 
   Size:       1471    3025
 
BsaBI GATnn'nnATC

Cuts at:      6    2010    4047       6 
   Size:       2004    2037     455

  Fragments arranged by size: 

                2037    2004     455
 
BsaHI Gr'CG_yC

Cuts at:   1313    1927    2175    2877    4373    1313 
   Size:        614     248     702    1496    1436

  Fragments arranged by size: 

                1496    1436     702     614     248
 
BsaWI w'CCGG_w

Cuts at:   1685    2206    2884    3158    3305    3716    1685 
   Size:        521     678     274     147     411    2465

  Fragments arranged by size: 

                2465     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1123    1153    1123 
   Size:         30    4466
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    134     298     301    1162    1165     134 
   Size:        164       3     861       3    3465

  Fragments arranged by size: 

                3465     861     164       3       3
 
BseYI C'CCAG_C

Cuts at:    855    1016    1355    1455    1921    2679    3256    3752     855 
   Size:        161     339     100     466     758     577     496    1599

  Fragments arranged by size: 

                1599     758     577     496     466     339     161     100
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1087    1087 
   Size:       4496
 
BsiEI CG_ry'CG

Cuts at:    104    2038    2084    3292     104 
   Size:       1934      46    1208    1308

  Fragments arranged by size: 

                1934    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    510     991    1091    1510    2288    2478    3270     510 
   Size:        481     100     419     778     190     792    1736

  Fragments arranged by size: 

                1736     792     778     481     419     190     100
 
BsiWI C'GTAC_G

Cuts at:    378     378 
   Size:       4496
 
Bsp1286I G_dGCh'C

Cuts at:    510     827     915     991    1081    1091    1462    1510    2121 
   Size:        317      88      76      90      10     371      48     611

Cuts at:   2121    2214    2288    2478    2540    3270     510 
   Size:         93      74     190      62     730    1736

  Fragments arranged by size: 

                1736     730     611     371     317     190      93      90
                  88      76      74      62      48      10
 
BspEI T'CCGG_A

Cuts at:   3716    3716 
   Size:       4496
 
BspHI T'CATG_A

Cuts at:   3672    3672 
   Size:       4496
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    779    1038    1336    1546    1760    2062    2443     779 
   Size:        259     298     210     214     302     381    2832

  Fragments arranged by size: 

                2832     381     302     298     259     214     210
 
BsrBI CCG'CTC

Cuts at:     50     954    2788    2842    4326    4491      50 
   Size:        904    1834      54    1484     165      55

  Fragments arranged by size: 

                1834    1484     904     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2408    2408 
   Size:       4496
 
BsrFI r'CCGG_y

Cuts at:   2494    2675    2884    2494 
   Size:        181     209    4106

  Fragments arranged by size: 

                4106     209     181
 
BssSI C'ACGA_G

Cuts at:    829    2767    3125     829 
   Size:       1938     358    2200

  Fragments arranged by size: 

                2200    1938     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    771     924    1873     771 
   Size:        153     949    3394

  Fragments arranged by size: 

                3394     949     153
 
BstBI TT'CG_AA

Cuts at:   2857    2857 
   Size:       4496
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1226    1226 
   Size:       4496
 
BstYI r'GATC_y

Cuts at:      1     370     495    1521    1988    2011    2346    2592    3604 
   Size:        369     125    1026     467      23     335     246    1012

Cuts at:   3604    3690    3702    4048       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1026    1012     467     449     369     346     335     246
                 125      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1054    1054 
   Size:       4496
 
BtgI C'CryG_G

Cuts at:    107     941     107 
   Size:        834    3662
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2446    2627    2790    2446 
   Size:        181     163    4152

  Fragments arranged by size: 

                4152     181     163
 
BtsI GCAGTG_nn'

Cuts at:    956    1108    1739     956 
   Size:        152     631    3713

  Fragments arranged by size: 

                3713     631     152
 
DraI TTT'AAA

Cuts at:   1495    1495 
   Size:       4496
 
DrdI GACnn_nn'nnGTC

Cuts at:   2202    3060    2202 
   Size:        858    3638
 
EaeI y'GGCC_r

Cuts at:    173     938    1373    1403    2081    2255    2646    2673    4018 
   Size:        765     435      30     678     174     391      27    1345

Cuts at:   4018     173 
   Size:        651

  Fragments arranged by size: 

                1345     765     678     651     435     391     174      30
                  27
 
EagI C'GGCC_G

Cuts at:   2081    2081 
   Size:       4496
 
EarI CTCTTCn'nnn_

Cuts at:    235     592     701    2519    2729     235 
   Size:        357     109    1818     210    2002

  Fragments arranged by size: 

                2002    1818     357     210     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    580    2704    3014    3160    4306     580 
   Size:       2124     310     146    1146     770

  Fragments arranged by size: 

                2124    1146     770     310     146
 
EcoICRI GAG'CTC

Cuts at:   1508    1508 
   Size:       4496
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    232     328     376     628     681    1051    1432    1498    2321 
   Size:         96      48     252      53     370     381      66     823

Cuts at:   2321    2753    3500    3768     232 
   Size:        432     747     268     960

  Fragments arranged by size: 

                 960     823     747     432     381     370     268     252
                  96      66      53      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:    832    1066     832 
   Size:        234    4262
 
EcoO109I rG'GnC_Cy

Cuts at:    339     768     972    1050    1058    1350    1635     339 
   Size:        429     204      78       8     292     285    3200

  Fragments arranged by size: 

                3200     429     292     285     204      78       8
 
EcoRI G'AATT_C

Cuts at:   1500    1500 
   Size:       4496
 
EcoRV GAT'ATC

Cuts at:    468     468 
   Size:       4496
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    603     633    2515     603 
   Size:         30    1882    2584

  Fragments arranged by size: 

                2584    1882      30
 
FspI TGC'GCA

Cuts at:    764    2277     764 
   Size:       1513    2983
 
HaeII r_GCGC'y

Cuts at:    863    1270    1930    2178    3200    4179     863 
   Size:        407     660     248    1022     979    1180

  Fragments arranged by size: 

                1180    1022     979     660     407     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    129     161    2338    2368    2370    2400     129 
   Size:         32    2177      30       2      30    2225

  Fragments arranged by size: 

                2225    2177      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1467    1535    1467 
   Size:         68    4428
 
KasI G'GCGC_C

Cuts at:   1926    2174    1926 
   Size:        248    4248
 
KpnI G_GTAC'C

Cuts at:   1516    1516 
   Size:       4496
 
MluI A'CGCG_T

Cuts at:   3929    3929 
   Size:       4496
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3167    3351    3167 
   Size:        184    4312
 
MscI TGG'CCA

Cuts at:    940    1375    1405    2257     940 
   Size:        435      30     852    3179

  Fragments arranged by size: 

                3179     852     435      30
 
MslI CAynn'nnrTG

Cuts at:   1084    2485    2612    1084 
   Size:       1401     127    2968

  Fragments arranged by size: 

                2968    1401     127
 
MspA1I CmG'CkG

Cuts at:   1020    1607    1921    2281    3294    3539    1020 
   Size:        587     314     360    1013     245    1977

  Fragments arranged by size: 

                1977    1013     587     360     314     245
 
NaeI GCC'GGC

Cuts at:   2677    2677 
   Size:       4496
 
NarI GG'CG_CC

Cuts at:   1927    2175    1927 
   Size:        248    4248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4496
 
NgoMIV G'CCGG_C

Cuts at:   2675    2675 
   Size:       4496
 
NspI r_CATG'y

Cuts at:    156     654    1549    2049    2956     156 
   Size:        498     895     500     907    1696

  Fragments arranged by size: 

                1696     907     895     500     498
 
PciI A'CATG_T

Cuts at:   2952    2952 
   Size:       4496
 
PflMI CCAn_nnn'nTGG

Cuts at:    753    1263    1401     753 
   Size:        510     138    3848

  Fragments arranged by size: 

                3848     510     138
 
PfoI T'CCnGG_A

Cuts at:    305    1445     305 
   Size:       1140    3356
 
PmeI GTTT'AAAC

Cuts at:   1495    1495 
   Size:       4496
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    418     450    1123    1155    3635    3667     418 
   Size:         32     673      32    2480      32    1247

  Fragments arranged by size: 

                2480    1247     673      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    768     972    1050    1058    1350     768 
   Size:        204      78       8     292    3914

  Fragments arranged by size: 

                3914     292     204      78       8
 
PstI C_TGCA'G

Cuts at:    369     920    1049    1193    1304    1385    1421    1543    2228 
   Size:        551     129     144     111      81      36     122     685

Cuts at:   2228     369 
   Size:       2637

  Fragments arranged by size: 

                2637     685     551     144     129     122     111      81
                  36
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4496
 
PvuII CAG'CTG

Cuts at:   1921    2281    1921 
   Size:        360    4136
 
RsrII CG'GwC_CG

Cuts at:   2691    2691 
   Size:       4496
 
SacI G_AGCT'C

Cuts at:   1510    1510 
   Size:       4496
 
SalI G'TCGA_C

Cuts at:   1533    1533 
   Size:       4496
 
SapI GCTCTTCn'nnn_

Cuts at:    235     592    2519    2729     235 
   Size:        357    1927     210    2002

  Fragments arranged by size: 

                2002    1927     357     210
 
SbfI CC_TGCA'GG

Cuts at:    369    1385    1543     369 
   Size:       1016     158    3322

  Fragments arranged by size: 

                3322    1016     158
 
ScaI AGT'ACT

Cuts at:   4013    4013 
   Size:       4496
 
SexAI A'CCwGG_T

Cuts at:   1121    1394    1121 
   Size:        273    4223
 
SfcI C'TryA_G

Cuts at:     33     365     916    1045    1189    1300    1381    1417    1539 
   Size:        332     551     129     144     111      81      36     122

Cuts at:   1539    2224    3217    3408      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     551     332     191     144     129
                 122     111      81      36
 
SfoI GGC'GCC

Cuts at:   1928    2176    1928 
   Size:        248    4248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4496
 
SmaI CCC'GGG

Cuts at:   1518    1518 
   Size:       4496
 
SmlI C'TyrA_G

Cuts at:    499    1651    2566    3058    3320     499 
   Size:       1152     915     492     262    1675

  Fragments arranged by size: 

                1675    1152     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4496
 
SphI G_CATG'C

Cuts at:    156    1549    2049     156 
   Size:       1393     500    2603

  Fragments arranged by size: 

                2603    1393     500
 
SspI AAT'ATT

Cuts at:    739     739 
   Size:       4496
 
StuI AGG'CCT

Cuts at:   1223    1223 
   Size:       4496
 
StyI C'CwwG_G

Cuts at:    107     881    1630    1984     107 
   Size:        774     749     354    2619

  Fragments arranged by size: 

                2619     774     749     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2079    2212    2671    2880    2079 
   Size:        133     459     209    3695

  Fragments arranged by size: 

                3695     459     209     133
 
TatI w'GTAC_w

Cuts at:   4011    4011 
   Size:       4496
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1298    1330    4013    4045    1298 
   Size:         32    2683      32    1749

  Fragments arranged by size: 

                2683    1749      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1123    2293    1123 
   Size:       1170    3326
 
XbaI T'CTAG_A

Cuts at:   1527    1527 
   Size:       4496
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    699     888    1991     699 
   Size:        189    1103    3204

  Fragments arranged by size: 

                3204    1103     189
 
XmaI C'CCGG_G

Cuts at:   1516    1516 
   Size:       4496
 
XmnI GAAnn'nnTTC

Cuts at:   1138    1138 
   Size:       4496
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     ApaI     AscI    AvrII     BaeI
    BfrBI     BglI    BmgBI     BmtI     BsmI    BsmBI    BsrGI   BssHII
   BstEII  BstZ17I     ClaI    CspCI   DraIII     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI     SfiI    SgrAI    SnaBI     SrfI     SwaI     XhoI     ZraI
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