Restriction map for
pF1KB5865
(Circular) (Six-base) MAPSORT of: pF1KB5865.seq Check: 8651 from: 1 to: 4496
pF1KB5865 4496 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1336 1336
Size: 4496
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 328 376 628 1051 1498 2321 2753 3500
Size: 96 48 252 423 447 823 432 747
Cuts at: 3500 3768 232
Size: 268 960
Fragments arranged by size:
960 823 747 447 432 423 268 252
96 48
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AleI CACnn'nnGTG
Cuts at: 1084 1084
Size: 4496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1102 1102
Size: 4496
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1123 1155 1123
Size: 32 4464
AlwNI CAG_nnn'CTG
Cuts at: 123 210 771 924 1029 1053 1308 1401 1452
Size: 87 561 153 105 24 255 93 51
Cuts at: 1452 1801 3368 123
Size: 349 1567 1251
Fragments arranged by size:
1567 1251 561 349 255 153 105 93
87 51 24
ApaLI G'TGCA_C
Cuts at: 506 3266 506
Size: 2760 1736
ApoI r'AATT_y
Cuts at: 517 1500 3849 4105 517
Size: 983 2349 256 908
Fragments arranged by size:
2349 983 908 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1036 1273 1516 1036
Size: 237 243 4016
Fragments arranged by size:
4016 243 237
BamHI G'GATC_C
Cuts at: 370 1521 370
Size: 1151 3345
BanI G'GyrC_C
Cuts at: 1512 1926 2174 2209 1512
Size: 414 248 35 3799
Fragments arranged by size:
3799 414 248 35
BanII G_rGCy'C
Cuts at: 827 915 1462 1510 2540 827
Size: 88 547 48 1030 2783
Fragments arranged by size:
2783 1030 547 88 48
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 560 781 560
Size: 221 4275
BbvCI CC'TCA_GC
Cuts at: 572 572
Size: 4496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1180 1214 1264 1298 1180
Size: 34 50 34 4378
Fragments arranged by size:
4378 50 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 671 800 2016 671
Size: 129 1216 3151
Fragments arranged by size:
3151 1216 129
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 1157 1608 1157
Size: 451 4045
Bme1580I G_kGCm'C
Cuts at: 510 1081 2121 2214 3270 510
Size: 571 1040 93 1056 1736
Fragments arranged by size:
1736 1056 1040 571 93
BmrI ACTGGGnnnn_n'
Cuts at: 407 1318 1708 1789 2124 4268 407
Size: 911 390 81 335 2144 635
Fragments arranged by size:
2144 911 635 390 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 681 1432 328
Size: 353 751 3392
Fragments arranged by size:
3392 751 353
Bpu10I CC'TnA_GC
Cuts at: 572 572
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1672 2551 3043 3341 484
Size: 1188 879 492 298 1639
Fragments arranged by size:
1639 1188 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 378 1849 378
Size: 1471 3025
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1313 1927 2175 2877 4373 1313
Size: 614 248 702 1496 1436
Fragments arranged by size:
1496 1436 702 614 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3158 3305 3716 1685
Size: 521 678 274 147 411 2465
Fragments arranged by size:
2465 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1123 1153 1123
Size: 30 4466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 134 298 301 1162 1165 134
Size: 164 3 861 3 3465
Fragments arranged by size:
3465 861 164 3 3
BseYI C'CCAG_C
Cuts at: 855 1016 1355 1455 1921 2679 3256 3752 855
Size: 161 339 100 466 758 577 496 1599
Fragments arranged by size:
1599 758 577 496 466 339 161 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1087
Size: 4496
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 510 991 1091 1510 2288 2478 3270 510
Size: 481 100 419 778 190 792 1736
Fragments arranged by size:
1736 792 778 481 419 190 100
BsiWI C'GTAC_G
Cuts at: 378 378
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 510 827 915 991 1081 1091 1462 1510 2121
Size: 317 88 76 90 10 371 48 611
Cuts at: 2121 2214 2288 2478 2540 3270 510
Size: 93 74 190 62 730 1736
Fragments arranged by size:
1736 730 611 371 317 190 93 90
88 76 74 62 48 10
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 779 1038 1336 1546 1760 2062 2443 779
Size: 259 298 210 214 302 381 2832
Fragments arranged by size:
2832 381 302 298 259 214 210
BsrBI CCG'CTC
Cuts at: 50 954 2788 2842 4326 4491 50
Size: 904 1834 54 1484 165 55
Fragments arranged by size:
1834 1484 904 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 2494 2675 2884 2494
Size: 181 209 4106
Fragments arranged by size:
4106 209 181
BssSI C'ACGA_G
Cuts at: 829 2767 3125 829
Size: 1938 358 2200
Fragments arranged by size:
2200 1938 358
BstAPI GCAn_nnn'nTGC
Cuts at: 771 924 1873 771
Size: 153 949 3394
Fragments arranged by size:
3394 949 153
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstXI CCAn_nnnn'nTGG
Cuts at: 1226 1226
Size: 4496
BstYI r'GATC_y
Cuts at: 1 370 495 1521 1988 2011 2346 2592 3604
Size: 369 125 1026 467 23 335 246 1012
Cuts at: 3604 3690 3702 4048 1
Size: 86 12 346 449
Fragments arranged by size:
1026 1012 467 449 369 346 335 246
125 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1054 1054
Size: 4496
BtgI C'CryG_G
Cuts at: 107 941 107
Size: 834 3662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 956 1108 1739 956
Size: 152 631 3713
Fragments arranged by size:
3713 631 152
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 173 938 1373 1403 2081 2255 2646 2673 4018
Size: 765 435 30 678 174 391 27 1345
Cuts at: 4018 173
Size: 651
Fragments arranged by size:
1345 765 678 651 435 391 174 30
27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 235 592 701 2519 2729 235
Size: 357 109 1818 210 2002
Fragments arranged by size:
2002 1818 357 210 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 580 2704 3014 3160 4306 580
Size: 2124 310 146 1146 770
Fragments arranged by size:
2124 1146 770 310 146
EcoICRI GAG'CTC
Cuts at: 1508 1508
Size: 4496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 328 376 628 681 1051 1432 1498 2321
Size: 96 48 252 53 370 381 66 823
Cuts at: 2321 2753 3500 3768 232
Size: 432 747 268 960
Fragments arranged by size:
960 823 747 432 381 370 268 252
96 66 53 48
EcoNI CCTnn'n_nnAGG
Cuts at: 832 1066 832
Size: 234 4262
EcoO109I rG'GnC_Cy
Cuts at: 339 768 972 1050 1058 1350 1635 339
Size: 429 204 78 8 292 285 3200
Fragments arranged by size:
3200 429 292 285 204 78 8
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
EcoRV GAT'ATC
Cuts at: 468 468
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 603 633 2515 603
Size: 30 1882 2584
Fragments arranged by size:
2584 1882 30
FspI TGC'GCA
Cuts at: 764 2277 764
Size: 1513 2983
HaeII r_GCGC'y
Cuts at: 863 1270 1930 2178 3200 4179 863
Size: 407 660 248 1022 979 1180
Fragments arranged by size:
1180 1022 979 660 407 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 129 161 2338 2368 2370 2400 129
Size: 32 2177 30 2 30 2225
Fragments arranged by size:
2225 2177 32 30 30 2
HincII GTy'rAC
Cuts at: 1467 1535 1467
Size: 68 4428
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 940 1375 1405 2257 940
Size: 435 30 852 3179
Fragments arranged by size:
3179 852 435 30
MslI CAynn'nnrTG
Cuts at: 1084 2485 2612 1084
Size: 1401 127 2968
Fragments arranged by size:
2968 1401 127
MspA1I CmG'CkG
Cuts at: 1020 1607 1921 2281 3294 3539 1020
Size: 587 314 360 1013 245 1977
Fragments arranged by size:
1977 1013 587 360 314 245
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4496
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NspI r_CATG'y
Cuts at: 156 654 1549 2049 2956 156
Size: 498 895 500 907 1696
Fragments arranged by size:
1696 907 895 500 498
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 753 1263 1401 753
Size: 510 138 3848
Fragments arranged by size:
3848 510 138
PfoI T'CCnGG_A
Cuts at: 305 1445 305
Size: 1140 3356
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 418 450 1123 1155 3635 3667 418
Size: 32 673 32 2480 32 1247
Fragments arranged by size:
2480 1247 673 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 768 972 1050 1058 1350 768
Size: 204 78 8 292 3914
Fragments arranged by size:
3914 292 204 78 8
PstI C_TGCA'G
Cuts at: 369 920 1049 1193 1304 1385 1421 1543 2228
Size: 551 129 144 111 81 36 122 685
Cuts at: 2228 369
Size: 2637
Fragments arranged by size:
2637 685 551 144 129 122 111 81
36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1921 2281 1921
Size: 360 4136
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1510 1510
Size: 4496
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 235 592 2519 2729 235
Size: 357 1927 210 2002
Fragments arranged by size:
2002 1927 357 210
SbfI CC_TGCA'GG
Cuts at: 369 1385 1543 369
Size: 1016 158 3322
Fragments arranged by size:
3322 1016 158
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SexAI A'CCwGG_T
Cuts at: 1121 1394 1121
Size: 273 4223
SfcI C'TryA_G
Cuts at: 33 365 916 1045 1189 1300 1381 1417 1539
Size: 332 551 129 144 111 81 36 122
Cuts at: 1539 2224 3217 3408 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 551 332 191 144 129
122 111 81 36
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 499 1651 2566 3058 3320 499
Size: 1152 915 492 262 1675
Fragments arranged by size:
1675 1152 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 156 1549 2049 156
Size: 1393 500 2603
Fragments arranged by size:
2603 1393 500
SspI AAT'ATT
Cuts at: 739 739
Size: 4496
StuI AGG'CCT
Cuts at: 1223 1223
Size: 4496
StyI C'CwwG_G
Cuts at: 107 881 1630 1984 107
Size: 774 749 354 2619
Fragments arranged by size:
2619 774 749 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2079 2212 2671 2880 2079
Size: 133 459 209 3695
Fragments arranged by size:
3695 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4011
Size: 4496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1298 1330 4013 4045 1298
Size: 32 2683 32 1749
Fragments arranged by size:
2683 1749 32 32
Tth111I GACn'n_nGTC
Cuts at: 1123 2293 1123
Size: 1170 3326
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 699 888 1991 699
Size: 189 1103 3204
Fragments arranged by size:
3204 1103 189
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
XmnI GAAnn'nnTTC
Cuts at: 1138 1138
Size: 4496
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI