Restriction map for
pF1KB5866
(Circular) (Six-base) MAPSORT of: pF1KB5866.seq Check: 2023 from: 1 to: 4586
pF1KB5866 4586 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 911 1624 911
Size: 713 3873
Acc65I G'GTAC_C
Cuts at: 1602 1602
Size: 4586
AclI AA'CG_TT
Cuts at: 4426 4426
Size: 4586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 1305 2411 2843 3590 3858 213
Size: 1092 1106 432 747 268 941
Fragments arranged by size:
1106 1092 941 747 432 268
AflIII A'CryG_T
Cuts at: 548 1938 3042 4019 548
Size: 1390 1104 977 1115
Fragments arranged by size:
1390 1115 1104 977
AgeI A'CCGG_T
Cuts at: 2974 2974
Size: 4586
AlwNI CAG_nnn'CTG
Cuts at: 1891 3458 1891
Size: 1567 3019
ApaLI G'TGCA_C
Cuts at: 3356 3356
Size: 4586
ApoI r'AATT_y
Cuts at: 1590 3939 4195 1590
Size: 2349 256 1981
Fragments arranged by size:
2349 1981 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4586
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
AvaI C'yCGr_G
Cuts at: 1606 1606
Size: 4586
BamHI G'GATC_C
Cuts at: 996 1611 996
Size: 615 3971
BanI G'GyrC_C
Cuts at: 1602 2016 2264 2299 1602
Size: 414 248 35 3889
Fragments arranged by size:
3889 414 248 35
BanII G_rGCy'C
Cuts at: 327 1600 2630 327
Size: 1273 1030 2283
Fragments arranged by size:
2283 1273 1030
BbeI G_GCGC'C
Cuts at: 2020 2268 2020
Size: 248 4338
BbsI GAAGACnn'nnnn_
Cuts at: 385 1394 385
Size: 1009 3577
BciVI GTATCCnnnnn_n'
Cuts at: 2484 3245 4586 2484
Size: 761 1341 2484
Fragments arranged by size:
2484 1341 761
BclI T'GATC_A
Cuts at: 2106 2106
Size: 4586
BglII A'GATC_T
Cuts at: 1214 2101 4138 1214
Size: 887 2037 1662
Fragments arranged by size:
2037 1662 887
BlpI GC'TnA_GC
Cuts at: 1698 1698
Size: 4586
Bme1580I G_kGCm'C
Cuts at: 237 2211 2304 3360 237
Size: 1974 93 1056 1463
Fragments arranged by size:
1974 1463 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1458 1798 1879 2214 4358 1458
Size: 340 81 335 2144 1686
Fragments arranged by size:
2144 1686 340 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 642 2953 642
Size: 2311 2275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 404
Size: 4586
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 2641 3133 3431 1762
Size: 879 492 298 2917
Fragments arranged by size:
2917 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4290 4290
Size: 4586
BsaAI yAC'GTr
Cuts at: 1328 1939 1328
Size: 611 3975
BsaBI GATnn'nnATC
Cuts at: 6 2100 4137 6
Size: 2094 2037 455
Fragments arranged by size:
2094 2037 455
BsaHI Gr'CG_yC
Cuts at: 2017 2265 2967 4463 2017
Size: 248 702 1496 2140
Fragments arranged by size:
2140 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1775 2296 2974 3248 3395 3806 1775
Size: 521 678 274 147 411 2555
Fragments arranged by size:
2555 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1145 1175 1145
Size: 30 4556
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 519 615 232
Size: 287 96 4203
Fragments arranged by size:
4203 287 96
BseYI C'CCAG_C
Cuts at: 277 2011 2769 3346 3842 277
Size: 1734 758 577 496 1021
Fragments arranged by size:
1734 1021 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2128 2174 3382 104
Size: 2024 46 1208 1308
Fragments arranged by size:
2024 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1600 2378 2568 3360 1600
Size: 778 190 792 2826
Fragments arranged by size:
2826 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 237 327 1600 2211 2304 2378 2568 2630 3360
Size: 90 1273 611 93 74 190 62 730
Cuts at: 3360 237
Size: 1463
Fragments arranged by size:
1463 1273 730 611 190 93 90 74
62
BspEI T'CCGG_A
Cuts at: 3806 3806
Size: 4586
BspHI T'CATG_A
Cuts at: 3762 3762
Size: 4586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1636 1850 2152 2533 1636
Size: 214 302 381 3689
Fragments arranged by size:
3689 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2878 2932 4416 4581 50
Size: 2828 54 1484 165 55
Fragments arranged by size:
2828 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2498 2498
Size: 4586
BsrFI r'CCGG_y
Cuts at: 1243 2584 2765 2974 1243
Size: 1341 181 209 2855
Fragments arranged by size:
2855 1341 209 181
BsrGI T'GTAC_A
Cuts at: 1188 1188
Size: 4586
BssSI C'ACGA_G
Cuts at: 2857 3215 2857
Size: 358 4228
BstAPI GCAn_nnn'nTGC
Cuts at: 785 1963 785
Size: 1178 3408
BstBI TT'CG_AA
Cuts at: 2947 2947
Size: 4586
BstYI r'GATC_y
Cuts at: 1 996 1214 1362 1611 2078 2101 2436 2682
Size: 995 218 148 249 467 23 335 246
Cuts at: 2682 3694 3780 3792 4138 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 995 467 449 346 335 249 246
218 148 86 23 12
BstZ17I GTA'TAC
Cuts at: 912 912
Size: 4586
Bsu36I CC'TnA_GG
Cuts at: 283 283
Size: 4586
BtgI C'CryG_G
Cuts at: 237 237
Size: 4586
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2536 2717 2880 2536
Size: 181 163 4242
Fragments arranged by size:
4242 181 163
BtsI GCAGTG_nn'
Cuts at: 1829 1829
Size: 4586
DraI TTT'AAA
Cuts at: 1585 1585
Size: 4586
DrdI GACnn_nn'nnGTC
Cuts at: 892 2292 3150 892
Size: 1400 858 2328
Fragments arranged by size:
2328 1400 858
EaeI y'GGCC_r
Cuts at: 2171 2345 2736 2763 4108 2171
Size: 174 391 27 1345 2649
Fragments arranged by size:
2649 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2171 2171
Size: 4586
EarI CTCTTCn'nnn_
Cuts at: 256 263 537 2609 2819 256
Size: 7 274 2072 210 2023
Fragments arranged by size:
2072 2023 274 210 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2794 3104 3250 4396 2794
Size: 310 146 1146 2984
Fragments arranged by size:
2984 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1598 1598
Size: 4586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 404 1305 2411 2843 3590 3858 213
Size: 191 901 1106 432 747 268 941
Fragments arranged by size:
1106 941 901 747 432 268 191
EcoO109I rG'GnC_Cy
Cuts at: 1114 1562 1725 1114
Size: 448 163 3975
Fragments arranged by size:
3975 448 163
EcoRI G'AATT_C
Cuts at: 1590 1590
Size: 4586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4586
FspI TGC'GCA
Cuts at: 2367 2367
Size: 4586
HaeII r_GCGC'y
Cuts at: 2020 2268 3290 4269 2020
Size: 248 1022 979 2337
Fragments arranged by size:
2337 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1252 1284 2428 2458 2460 2490 1252
Size: 32 1144 30 2 30 3348
Fragments arranged by size:
3348 1144 32 30 30 2
HincII GTy'rAC
Cuts at: 207 978 1355 1625 207
Size: 771 377 270 3168
Fragments arranged by size:
3168 771 377 270
HpaI GTT'AAC
Cuts at: 1355 1355
Size: 4586
KasI G'GCGC_C
Cuts at: 2016 2264 2016
Size: 248 4338
KpnI G_GTAC'C
Cuts at: 1606 1606
Size: 4586
MluI A'CGCG_T
Cuts at: 4019 4019
Size: 4586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3257 3441 3257
Size: 184 4402
MscI TGG'CCA
Cuts at: 2347 2347
Size: 4586
MslI CAynn'nnrTG
Cuts at: 2575 2702 2575
Size: 127 4459
MspA1I CmG'CkG
Cuts at: 343 1275 1697 2011 2371 3384 3629 343
Size: 932 422 314 360 1013 245 1300
Fragments arranged by size:
1300 1013 932 422 360 314 245
NaeI GCC'GGC
Cuts at: 2767 2767
Size: 4586
NarI GG'CG_CC
Cuts at: 2017 2265 2017
Size: 248 4338
NgoMIV G'CCGG_C
Cuts at: 2765 2765
Size: 4586
NspI r_CATG'y
Cuts at: 552 1317 1639 2139 3046 552
Size: 765 322 500 907 2092
Fragments arranged by size:
2092 907 765 500 322
PciI A'CATG_T
Cuts at: 548 3042 548
Size: 2494 2092
PfoI T'CCnGG_A
Cuts at: 710 710
Size: 4586
PmeI GTTT'AAAC
Cuts at: 1585 1585
Size: 4586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4554
PpuMI rG'GwC_Cy
Cuts at: 1114 1114
Size: 4586
PstI C_TGCA'G
Cuts at: 1633 2318 1633
Size: 685 3901
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4586
PvuII CAG'CTG
Cuts at: 343 1275 2011 2371 343
Size: 932 736 360 2558
Fragments arranged by size:
2558 932 736 360
RsrII CG'GwC_CG
Cuts at: 2781 2781
Size: 4586
SacI G_AGCT'C
Cuts at: 1600 1600
Size: 4586
SalI G'TCGA_C
Cuts at: 1623 1623
Size: 4586
SanDI GG'GwC_CC
Cuts at: 1114 1114
Size: 4586
SapI GCTCTTCn'nnn_
Cuts at: 2609 2819 2609
Size: 210 4376
SbfI CC_TGCA'GG
Cuts at: 1633 1633
Size: 4586
ScaI AGT'ACT
Cuts at: 622 4103 622
Size: 3481 1105
SexAI A'CCwGG_T
Cuts at: 1062 1062
Size: 4586
SfcI C'TryA_G
Cuts at: 33 428 1629 2314 3307 3498 33
Size: 395 1201 685 993 191 1121
Fragments arranged by size:
1201 1121 993 685 395 191
SfoI GGC'GCC
Cuts at: 2018 2266 2018
Size: 248 4338
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
SmaI CCC'GGG
Cuts at: 1608 1608
Size: 4586
SmlI C'TyrA_G
Cuts at: 1741 2656 3148 3410 1741
Size: 915 492 262 2917
Fragments arranged by size:
2917 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4586
SphI G_CATG'C
Cuts at: 1639 2139 1639
Size: 500 4086
SspI AAT'ATT
Cuts at: 772 804 1072 772
Size: 32 268 4286
Fragments arranged by size:
4286 268 32
StuI AGG'CCT
Cuts at: 288 288
Size: 4586
StyI C'CwwG_G
Cuts at: 198 1720 2074 198
Size: 1522 354 2710
Fragments arranged by size:
2710 1522 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2169 2302 2761 2970 2169
Size: 133 459 209 3785
Fragments arranged by size:
3785 459 209 133
TatI w'GTAC_w
Cuts at: 545 620 1188 1460 4101 545
Size: 75 568 272 2641 1030
Fragments arranged by size:
2641 1030 568 272 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 837 869 932 964 4103 4135 837
Size: 32 63 32 3139 32 1288
Fragments arranged by size:
3139 1288 63 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2383 2383
Size: 4586
XbaI T'CTAG_A
Cuts at: 1617 1617
Size: 4586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1070 2081 114
Size: 956 1011 2619
Fragments arranged by size:
2619 1011 956
XmaI C'CCGG_G
Cuts at: 1606 1606
Size: 4586
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI