Restriction map for
pF1KB5869
(Circular) (Six-base) MAPSORT of: pF1KB5869.seq Check: 577 from: 1 to: 4502
pF1KB5869 4502 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 566 1540 566
Size: 974 3528
Acc65I G'GTAC_C
Cuts at: 1518 1518
Size: 4502
AclI AA'CG_TT
Cuts at: 4342 4342
Size: 4502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2759 3506 3774 2327
Size: 432 747 268 3055
Fragments arranged by size:
3055 747 432 268
AflIII A'CryG_T
Cuts at: 626 628 1101 1854 2958 3935 626
Size: 2 473 753 1104 977 1193
Fragments arranged by size:
1193 1104 977 753 473 2
AgeI A'CCGG_T
Cuts at: 2890 2890
Size: 4502
AleI CACnn'nnGTG
Cuts at: 521 521
Size: 4502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 472 504 472
Size: 32 4470
AlwNI CAG_nnn'CTG
Cuts at: 1807 3374 1807
Size: 1567 2935
ApaI G_GGCC'C
Cuts at: 343 343
Size: 4502
ApaLI G'TGCA_C
Cuts at: 3272 3272
Size: 4502
ApoI r'AATT_y
Cuts at: 1506 3855 4111 1506
Size: 2349 256 1897
Fragments arranged by size:
2349 1897 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
AvaI C'yCGr_G
Cuts at: 463 1522 463
Size: 1059 3443
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 239 272 239
Size: 33 4469
BamHI G'GATC_C
Cuts at: 1527 1527
Size: 4502
BanI G'GyrC_C
Cuts at: 1518 1932 2180 2215 1518
Size: 414 248 35 3805
Fragments arranged by size:
3805 414 248 35
BanII G_rGCy'C
Cuts at: 193 310 343 1516 2546 193
Size: 117 33 1173 1030 2149
Fragments arranged by size:
2149 1173 1030 117 33
BbeI G_GCGC'C
Cuts at: 1936 2184 1936
Size: 248 4254
BbsI GAAGACnn'nnnn_
Cuts at: 809 809
Size: 4502
BciVI GTATCCnnnnn_n'
Cuts at: 1343 2400 3161 4502 1343
Size: 1057 761 1341 1343
Fragments arranged by size:
1343 1341 1057 761
BclI T'GATC_A
Cuts at: 2022 2022
Size: 4502
BglI GCCn_nnn'nGGC
Cuts at: 173 173
Size: 4502
BglII A'GATC_T
Cuts at: 2017 4054 2017
Size: 2037 2465
BlpI GC'TnA_GC
Cuts at: 1614 1614
Size: 4502
Bme1580I G_kGCm'C
Cuts at: 264 343 1122 2127 2220 3276 264
Size: 79 779 1005 93 1056 1490
Fragments arranged by size:
1490 1056 1005 779 93 79
BmrI ACTGGGnnnn_n'
Cuts at: 247 1219 1714 1795 2130 4274 247
Size: 972 495 81 335 2144 475
Fragments arranged by size:
2144 972 495 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 1429 353
Size: 1076 3426
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 1678 2557 3049 3347 1330
Size: 348 879 492 298 2485
Fragments arranged by size:
2485 879 492 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 1471 4206 1471
Size: 2735 1767
BsaAI yAC'GTr
Cuts at: 629 1855 629
Size: 1226 3276
BsaBI GATnn'nnATC
Cuts at: 6 2016 4053 6
Size: 2010 2037 455
Fragments arranged by size:
2037 2010 455
BsaHI Gr'CG_yC
Cuts at: 1933 2181 2883 4379 1933
Size: 248 702 1496 2056
Fragments arranged by size:
2056 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1109 1335 1691 2212 2890 3164 3311 3722 1109
Size: 226 356 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 356 274 226 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 765 765
Size: 4502
BseYI C'CCAG_C
Cuts at: 235 394 1129 1377 1927 2685 3262 3758 235
Size: 159 735 248 550 758 577 496 979
Fragments arranged by size:
979 758 735 577 550 496 248 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 1090 299
Size: 791 3711
BsiEI CG_ry'CG
Cuts at: 104 2044 2090 3298 104
Size: 1940 46 1208 1308
Fragments arranged by size:
1940 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 357 1415 1516 2294 2484 3276 357
Size: 1058 101 778 190 792 1583
Fragments arranged by size:
1583 1058 792 778 190 101
Bsp1286I G_dGCh'C
Cuts at: 193 264 310 343 357 1122 1415 1516 2127
Size: 71 46 33 14 765 293 101 611
Cuts at: 2127 2220 2294 2484 2546 3276 193
Size: 93 74 190 62 730 1419
Fragments arranged by size:
1419 765 730 611 293 190 101 93
74 71 62 46 33 14
BspEI T'CCGG_A
Cuts at: 1335 3722 1335
Size: 2387 2115
BspHI T'CATG_A
Cuts at: 131 798 3678 131
Size: 667 2880 955
Fragments arranged by size:
2880 955 667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1340 1552 1766 2068 2449 1340
Size: 212 214 302 381 3393
Fragments arranged by size:
3393 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 1058 2794 2848 4332 4497 50
Size: 1008 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 1008 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2414 2414
Size: 4502
BsrFI r'CCGG_y
Cuts at: 2500 2681 2890 2500
Size: 181 209 4112
Fragments arranged by size:
4112 209 181
BssSI C'ACGA_G
Cuts at: 2773 3131 2773
Size: 358 4144
BstAPI GCAn_nnn'nTGC
Cuts at: 677 784 1879 677
Size: 107 1095 3300
Fragments arranged by size:
3300 1095 107
BstBI TT'CG_AA
Cuts at: 2863 2863
Size: 4502
BstXI CCAn_nnnn'nTGG
Cuts at: 1129 1129
Size: 4502
BstYI r'GATC_y
Cuts at: 1 502 541 1527 1994 2017 2352 2598 3610
Size: 501 39 986 467 23 335 246 1012
Cuts at: 3610 3696 3708 4054 1
Size: 86 12 346 449
Fragments arranged by size:
1012 986 501 467 449 346 335 246
86 39 23 12
BstZ17I GTA'TAC
Cuts at: 567 567
Size: 4502
Bsu36I CC'TnA_GG
Cuts at: 348 546 348
Size: 198 4304
BtgI C'CryG_G
Cuts at: 265 335 1091 265
Size: 70 756 3676
Fragments arranged by size:
3676 756 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2452 2633 2796 2452
Size: 181 163 4158
Fragments arranged by size:
4158 181 163
BtsI GCAGTG_nn'
Cuts at: 287 1122 1745 287
Size: 835 623 3044
Fragments arranged by size:
3044 835 623
DraI TTT'AAA
Cuts at: 1501 1501
Size: 4502
DrdI GACnn_nn'nnGTC
Cuts at: 2208 3066 2208
Size: 858 3644
EaeI y'GGCC_r
Cuts at: 165 1088 2087 2261 2652 2679 4024 165
Size: 923 999 174 391 27 1345 643
Fragments arranged by size:
1345 999 923 643 391 174 27
EagI C'GGCC_G
Cuts at: 2087 2087
Size: 4502
EarI CTCTTCn'nnn_
Cuts at: 715 1423 2525 2735 715
Size: 708 1102 210 2482
Fragments arranged by size:
2482 1102 708 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2710 3020 3166 4312 2710
Size: 310 146 1146 2900
Fragments arranged by size:
2900 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1514 1514
Size: 4502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 1429 2327 2759 3506 3774 353
Size: 1076 898 432 747 268 1081
Fragments arranged by size:
1081 1076 898 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 287 615 1254 1641 287
Size: 328 639 387 3148
Fragments arranged by size:
3148 639 387 328
EcoRI G'AATT_C
Cuts at: 1506 1506
Size: 4502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 853 2521 853
Size: 1668 2834
FspI TGC'GCA
Cuts at: 2283 2283
Size: 4502
HaeII r_GCGC'y
Cuts at: 367 1936 2184 3206 4185 367
Size: 1569 248 1022 979 684
Fragments arranged by size:
1569 1022 979 684 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 529 561 2344 2374 2376 2406 529
Size: 32 1783 30 2 30 2625
Fragments arranged by size:
2625 1783 32 30 30 2
HincII GTy'rAC
Cuts at: 417 1541 417
Size: 1124 3378
KasI G'GCGC_C
Cuts at: 1932 2180 1932
Size: 248 4254
KpnI G_GTAC'C
Cuts at: 1522 1522
Size: 4502
MluI A'CGCG_T
Cuts at: 3935 3935
Size: 4502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1184 1286 1454 3173 3357 1184
Size: 102 168 1719 184 2329
Fragments arranged by size:
2329 1719 184 168 102
MscI TGG'CCA
Cuts at: 167 1090 2263 167
Size: 923 1173 2406
Fragments arranged by size:
2406 1173 923
MslI CAynn'nnrTG
Cuts at: 521 715 811 877 1192 1468 2491 2618 521
Size: 194 96 66 315 276 1023 127 2405
Fragments arranged by size:
2405 1023 315 276 194 127 96 66
MspA1I CmG'CkG
Cuts at: 677 1485 1613 1927 2287 3300 3545 677
Size: 808 128 314 360 1013 245 1634
Fragments arranged by size:
1634 1013 808 360 314 245 128
NaeI GCC'GGC
Cuts at: 2683 2683
Size: 4502
NarI GG'CG_CC
Cuts at: 1933 2181 1933
Size: 248 4254
NcoI C'CATG_G
Cuts at: 335 335
Size: 4502
NdeI CA'TA_TG
Cuts at: 913 913
Size: 4502
NgoMIV G'CCGG_C
Cuts at: 2681 2681
Size: 4502
NspI r_CATG'y
Cuts at: 810 1105 1191 1555 2055 2962 810
Size: 295 86 364 500 907 2350
Fragments arranged by size:
2350 907 500 364 295 86
PasI CC'CwG_GG
Cuts at: 303 303
Size: 4502
PciI A'CATG_T
Cuts at: 1101 2958 1101
Size: 1857 2645
PfoI T'CCnGG_A
Cuts at: 369 369
Size: 4502
PmeI GTTT'AAAC
Cuts at: 1501 1501
Size: 4502
PmlI CAC'GTG
Cuts at: 629 629
Size: 4502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4470
PpuMI rG'GwC_Cy
Cuts at: 287 615 287
Size: 328 4174
PshAI GACnn'nnGTC
Cuts at: 662 662
Size: 4502
PspOMI G'GGCC_C
Cuts at: 339 339
Size: 4502
PstI C_TGCA'G
Cuts at: 1490 1549 2234 1490
Size: 59 685 3758
Fragments arranged by size:
3758 685 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4502
PvuII CAG'CTG
Cuts at: 1927 2287 1927
Size: 360 4142
RsrII CG'GwC_CG
Cuts at: 2697 2697
Size: 4502
SacI G_AGCT'C
Cuts at: 1516 1516
Size: 4502
SalI G'TCGA_C
Cuts at: 1539 1539
Size: 4502
SanDI GG'GwC_CC
Cuts at: 287 287
Size: 4502
SapI GCTCTTCn'nnn_
Cuts at: 2525 2735 2525
Size: 210 4292
SbfI CC_TGCA'GG
Cuts at: 1549 1549
Size: 4502
ScaI AGT'ACT
Cuts at: 685 4019 685
Size: 3334 1168
SexAI A'CCwGG_T
Cuts at: 998 998
Size: 4502
SfcI C'TryA_G
Cuts at: 33 1486 1545 2230 3223 3414 33
Size: 1453 59 685 993 191 1121
Fragments arranged by size:
1453 1121 993 685 191 59
SfoI GGC'GCC
Cuts at: 1934 2182 1934
Size: 248 4254
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
SmaI CCC'GGG
Cuts at: 465 1524 465
Size: 1059 3443
SmlI C'TyrA_G
Cuts at: 1309 1657 2572 3064 3326 1309
Size: 348 915 492 262 2485
Fragments arranged by size:
2485 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4502
SphI G_CATG'C
Cuts at: 1555 2055 1555
Size: 500 4002
StuI AGG'CCT
Cuts at: 1315 1315
Size: 4502
StyI C'CwwG_G
Cuts at: 335 989 1250 1636 1990 335
Size: 654 261 386 354 2847
Fragments arranged by size:
2847 654 386 354 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 408 2085 2218 2677 2886 408
Size: 1677 133 459 209 2024
Fragments arranged by size:
2024 1677 459 209 133
TatI w'GTAC_w
Cuts at: 683 1230 4017 683
Size: 547 2787 1168
Fragments arranged by size:
2787 1168 547
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 261 293 4019 4051 261
Size: 32 3726 32 712
Fragments arranged by size:
3726 712 32 32
Tth111I GACn'n_nGTC
Cuts at: 1000 2299 1000
Size: 1299 3203
XbaI T'CTAG_A
Cuts at: 825 1533 825
Size: 708 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1997 1997
Size: 4502
XmaI C'CCGG_G
Cuts at: 463 1522 463
Size: 1059 3443
XmnI GAAnn'nnTTC
Cuts at: 120 120
Size: 4502
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PflMI PsiI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI