Restriction map for
pF1KB5874
(Circular) (Six-base) MAPSORT of: pF1KB5874.seq Check: 2908 from: 1 to: 4595
pF1KB5874 4595 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1633 1633
Size: 4595
Acc65I G'GTAC_C
Cuts at: 1611 1611
Size: 4595
AclI AA'CG_TT
Cuts at: 4435 4435
Size: 4595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 617 947 2420 2852 3599 3867 226
Size: 391 330 1473 432 747 268 954
Fragments arranged by size:
1473 954 747 432 391 330 268
AfeI AGC'GCT
Cuts at: 166 891 166
Size: 725 3870
AflIII A'CryG_T
Cuts at: 1947 3051 4028 1947
Size: 1104 977 2514
Fragments arranged by size:
2514 1104 977
AgeI A'CCGG_T
Cuts at: 2983 2983
Size: 4595
AlwNI CAG_nnn'CTG
Cuts at: 1900 3467 1900
Size: 1567 3028
ApaLI G'TGCA_C
Cuts at: 3365 3365
Size: 4595
ApoI r'AATT_y
Cuts at: 435 544 1599 3948 4204 435
Size: 109 1055 2349 256 826
Fragments arranged by size:
2349 1055 826 256 109
AseI AT'TA_AT
Cuts at: 20 20
Size: 4595
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
AvaI C'yCGr_G
Cuts at: 146 1615 146
Size: 1469 3126
BamHI G'GATC_C
Cuts at: 1620 1620
Size: 4595
BanI G'GyrC_C
Cuts at: 1411 1611 2025 2273 2308 1411
Size: 200 414 248 35 3698
Fragments arranged by size:
3698 414 248 200 35
BanII G_rGCy'C
Cuts at: 784 856 1609 2639 784
Size: 72 753 1030 2740
Fragments arranged by size:
2740 1030 753 72
BbeI G_GCGC'C
Cuts at: 2029 2277 2029
Size: 248 4347
BbsI GAAGACnn'nnnn_
Cuts at: 635 1304 635
Size: 669 3926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4561
BciVI GTATCCnnnnn_n'
Cuts at: 2493 3254 4595 2493
Size: 761 1341 2493
Fragments arranged by size:
2493 1341 761
BclI T'GATC_A
Cuts at: 2115 2115
Size: 4595
BglI GCCn_nnn'nGGC
Cuts at: 731 731
Size: 4595
BglII A'GATC_T
Cuts at: 763 1321 1567 2110 4147 763
Size: 558 246 543 2037 1211
Fragments arranged by size:
2037 1211 558 543 246
BlpI GC'TnA_GC
Cuts at: 1707 1707
Size: 4595
Bme1580I G_kGCm'C
Cuts at: 2220 2313 3369 2220
Size: 93 1056 3446
Fragments arranged by size:
3446 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1308 1807 1888 2223 4367 1308
Size: 499 81 335 2144 1536
Fragments arranged by size:
2144 1536 499 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 782 2962 782
Size: 2180 2415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 648 1470 214
Size: 434 822 3339
Fragments arranged by size:
3339 822 434
Bpu10I CC'TnA_GC
Cuts at: 133 133
Size: 4595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1566 1771 2650 3142 3440 1566
Size: 205 879 492 298 2721
Fragments arranged by size:
2721 879 492 298 205
BsaI GGTCTCn'nnnn_
Cuts at: 692 1417 4299 692
Size: 725 2882 988
Fragments arranged by size:
2882 988 725
BsaAI yAC'GTr
Cuts at: 1948 1948
Size: 4595
BsaBI GATnn'nnATC
Cuts at: 6 1326 1566 2109 4146 6
Size: 1320 240 543 2037 455
Fragments arranged by size:
2037 1320 543 455 240
BsaHI Gr'CG_yC
Cuts at: 651 2026 2274 2976 4472 651
Size: 1375 248 702 1496 774
Fragments arranged by size:
1496 1375 774 702 248
BsaWI w'CCGG_w
Cuts at: 1784 2305 2983 3257 3404 3815 1784
Size: 521 678 274 147 411 2564
Fragments arranged by size:
2564 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 424 454 804 834 1002 1032 1414 1444 424
Size: 30 350 30 168 30 382 30 3575
Fragments arranged by size:
3575 382 350 168 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1041 1051 1054 1207 1248 1406 1041
Size: 10 3 153 41 158 4230
Fragments arranged by size:
4230 158 153 41 10 3
BseYI C'CCAG_C
Cuts at: 856 2020 2778 3355 3851 856
Size: 1164 758 577 496 1600
Fragments arranged by size:
1600 1164 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 471
Size: 4595
BsiEI CG_ry'CG
Cuts at: 104 129 2137 2183 3391 104
Size: 25 2008 46 1208 1308
Fragments arranged by size:
2008 1308 1208 46 25
BsiHKAI G_wGCw'C
Cuts at: 1609 2387 2577 3369 1609
Size: 778 190 792 2835
Fragments arranged by size:
2835 792 778 190
BsmI GAATG_Cn'
Cuts at: 1410 1410
Size: 4595
Bsp1286I G_dGCh'C
Cuts at: 784 856 1609 2220 2313 2387 2577 2639 3369
Size: 72 753 611 93 74 190 62 730
Cuts at: 3369 784
Size: 2010
Fragments arranged by size:
2010 753 730 611 190 93 74 72
62
BspEI T'CCGG_A
Cuts at: 3815 3815
Size: 4595
BspHI T'CATG_A
Cuts at: 3771 3771
Size: 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1645 1859 2161 2542 1645
Size: 214 302 381 3698
Fragments arranged by size:
3698 381 302 214
BsrBI CCG'CTC
Cuts at: 50 788 1310 1334 1350 2887 2941 4425 4590
Size: 738 522 24 16 1537 54 1484 165
Cuts at: 4590 50
Size: 55
Fragments arranged by size:
1537 1484 738 522 165 55 54 24
16
BsrDI GCAATG_nn'
Cuts at: 2507 2507
Size: 4595
BsrFI r'CCGG_y
Cuts at: 653 2593 2774 2983 653
Size: 1940 181 209 2265
Fragments arranged by size:
2265 1940 209 181
BsrGI T'GTAC_A
Cuts at: 119 119
Size: 4595
BssSI C'ACGA_G
Cuts at: 2866 3224 2866
Size: 358 4237
BstAPI GCAn_nnn'nTGC
Cuts at: 256 1972 256
Size: 1716 2879
BstBI TT'CG_AA
Cuts at: 2956 2956
Size: 4595
BstXI CCAn_nnnn'nTGG
Cuts at: 892 892
Size: 4595
BstYI r'GATC_y
Cuts at: 1 188 201 763 935 1321 1441 1531 1567
Size: 187 13 562 172 386 120 90 36
Cuts at: 1567 1620 2087 2110 2445 2691 3703 3789 3801
Size: 53 467 23 335 246 1012 86 12
Cuts at: 3801 4147 1
Size: 346 449
Fragments arranged by size:
1012 562 467 449 386 346 335 246
187 172 120 90 86 53 36 23
13 12
BtgI C'CryG_G
Cuts at: 112 318 325 112
Size: 206 7 4382
Fragments arranged by size:
4382 206 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 170 2545 2726 2889 170
Size: 2375 181 163 1876
Fragments arranged by size:
2375 1876 181 163
BtsI GCAGTG_nn'
Cuts at: 363 1838 363
Size: 1475 3120
DraI TTT'AAA
Cuts at: 1594 1594
Size: 4595
DrdI GACnn_nn'nnGTC
Cuts at: 2301 3159 2301
Size: 858 3737
EaeI y'GGCC_r
Cuts at: 126 381 2180 2354 2745 2772 4117 126
Size: 255 1799 174 391 27 1345 604
Fragments arranged by size:
1799 1345 604 391 255 174 27
EagI C'GGCC_G
Cuts at: 126 2180 126
Size: 2054 2541
EarI CTCTTCn'nnn_
Cuts at: 923 1075 1174 2618 2828 923
Size: 152 99 1444 210 2690
Fragments arranged by size:
2690 1444 210 152 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1078 2803 3113 3259 4405 1078
Size: 1725 310 146 1146 1268
Fragments arranged by size:
1725 1268 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1607 1607
Size: 4595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 226 617 648 947 1470 2420 2852 3599
Size: 12 391 31 299 523 950 432 747
Cuts at: 3599 3867 214
Size: 268 942
Fragments arranged by size:
950 942 747 523 432 391 299 268
31 12
EcoO109I rG'GnC_Cy
Cuts at: 1574 1734 1574
Size: 160 4435
EcoRI G'AATT_C
Cuts at: 1599 1599
Size: 4595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4595
FspI TGC'GCA
Cuts at: 2376 2376
Size: 4595
HaeII r_GCGC'y
Cuts at: 168 893 2029 2277 3299 4278 168
Size: 725 1136 248 1022 979 485
Fragments arranged by size:
1136 1022 979 725 485 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 424 456 804 836 960 990 992 1002 1022
Size: 32 348 32 124 30 2 10 20
Cuts at: 1022 1034 1188 1220 1371 1403 1414 1446 2437
Size: 12 154 32 151 32 11 32 991
Cuts at: 2437 2467 2469 2499 424
Size: 30 2 30 2520
Fragments arranged by size:
2520 991 348 154 151 124 32 32
32 32 32 30 30 30 20 12
11 10 2 2
HincII GTy'rAC
Cuts at: 1634 1634
Size: 4595
KasI G'GCGC_C
Cuts at: 2025 2273 2025
Size: 248 4347
KpnI G_GTAC'C
Cuts at: 1615 1615
Size: 4595
MluI A'CGCG_T
Cuts at: 4028 4028
Size: 4595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 566 802 988 1078 3266 3450 566
Size: 236 186 90 2188 184 1711
Fragments arranged by size:
2188 1711 236 186 184 90
MscI TGG'CCA
Cuts at: 2356 2356
Size: 4595
MslI CAynn'nnrTG
Cuts at: 2584 2711 2584
Size: 127 4468
MspA1I CmG'CkG
Cuts at: 114 320 455 1706 2020 2380 3393 3638 114
Size: 206 135 1251 314 360 1013 245 1071
Fragments arranged by size:
1251 1071 1013 360 314 245 206 135
NaeI GCC'GGC
Cuts at: 2776 2776
Size: 4595
NarI GG'CG_CC
Cuts at: 2026 2274 2026
Size: 248 4347
NgoMIV G'CCGG_C
Cuts at: 2774 2774
Size: 4595
NspI r_CATG'y
Cuts at: 316 1360 1648 2148 3055 316
Size: 1044 288 500 907 1856
Fragments arranged by size:
1856 1044 907 500 288
PciI A'CATG_T
Cuts at: 3051 3051
Size: 4595
PfoI T'CCnGG_A
Cuts at: 191 790 191
Size: 599 3996
PmeI GTTT'AAAC
Cuts at: 1594 1594
Size: 4595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4563
PpuMI rG'GwC_Cy
Cuts at: 1574 1574
Size: 4595
PshAI GACnn'nnGTC
Cuts at: 654 654
Size: 4595
PstI C_TGCA'G
Cuts at: 1642 2327 1642
Size: 685 3910
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4595
PvuII CAG'CTG
Cuts at: 455 2020 2380 455
Size: 1565 360 2670
Fragments arranged by size:
2670 1565 360
RsrII CG'GwC_CG
Cuts at: 2790 2790
Size: 4595
SacI G_AGCT'C
Cuts at: 1609 1609
Size: 4595
SacII CC_GC'GG
Cuts at: 115 321 115
Size: 206 4389
SalI G'TCGA_C
Cuts at: 1632 1632
Size: 4595
SapI GCTCTTCn'nnn_
Cuts at: 1075 2618 2828 1075
Size: 1543 210 2842
Fragments arranged by size:
2842 1543 210
SbfI CC_TGCA'GG
Cuts at: 1642 1642
Size: 4595
ScaI AGT'ACT
Cuts at: 4112 4112
Size: 4595
SfcI C'TryA_G
Cuts at: 33 1638 2323 3316 3507 33
Size: 1605 685 993 191 1121
Fragments arranged by size:
1605 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2027 2275 2027
Size: 248 4347
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
SmaI CCC'GGG
Cuts at: 148 1617 148
Size: 1469 3126
SmlI C'TyrA_G
Cuts at: 1581 1750 2665 3157 3419 1581
Size: 169 915 492 262 2757
Fragments arranged by size:
2757 915 492 262 169
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4595
SphI G_CATG'C
Cuts at: 316 1648 2148 316
Size: 1332 500 2763
Fragments arranged by size:
2763 1332 500
StyI C'CwwG_G
Cuts at: 1729 2083 1729
Size: 354 4241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 758 1508 2178 2311 2770 2979 758
Size: 750 670 133 459 209 2374
Fragments arranged by size:
2374 750 670 459 209 133
TatI w'GTAC_w
Cuts at: 119 416 4110 119
Size: 297 3694 604
Fragments arranged by size:
3694 604 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1570 1602 4112 4144 1570
Size: 32 2510 32 2021
Fragments arranged by size:
2510 2021 32 32
Tth111I GACn'n_nGTC
Cuts at: 952 1428 2392 952
Size: 476 964 3155
Fragments arranged by size:
3155 964 476
XbaI T'CTAG_A
Cuts at: 1528 1564 1626 1528
Size: 36 62 4497
Fragments arranged by size:
4497 62 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 2090
Size: 4595
XmaI C'CCGG_G
Cuts at: 146 1615 146
Size: 1469 3126
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI