Restriction map for
pF1KB5878
(Circular) (Six-base) MAPSORT of: pF1KB5878.seq Check: 5777 from: 1 to: 4598
pF1KB5878 4598 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1636 1636
Size: 4598
Acc65I G'GTAC_C
Cuts at: 1614 1614
Size: 4598
AclI AA'CG_TT
Cuts at: 4438 4438
Size: 4598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 2423 2855 3602 3870 985
Size: 1438 432 747 268 1713
Fragments arranged by size:
1713 1438 747 432 268
AflIII A'CryG_T
Cuts at: 186 1950 3054 4031 186
Size: 1764 1104 977 753
Fragments arranged by size:
1764 1104 977 753
AgeI A'CCGG_T
Cuts at: 2986 2986
Size: 4598
AlwNI CAG_nnn'CTG
Cuts at: 1903 3470 1903
Size: 1567 3031
ApaLI G'TGCA_C
Cuts at: 3368 3368
Size: 4598
ApoI r'AATT_y
Cuts at: 126 1252 1577 1602 3951 4207 126
Size: 1126 325 25 2349 256 517
Fragments arranged by size:
2349 1126 517 325 256 25
AseI AT'TA_AT
Cuts at: 20 1279 1427 20
Size: 1259 148 3191
Fragments arranged by size:
3191 1259 148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
AvaI C'yCGr_G
Cuts at: 1618 1618
Size: 4598
BamHI G'GATC_C
Cuts at: 1623 1623
Size: 4598
BanI G'GyrC_C
Cuts at: 1614 2028 2276 2311 1614
Size: 414 248 35 3901
Fragments arranged by size:
3901 414 248 35
BanII G_rGCy'C
Cuts at: 1612 2642 1612
Size: 1030 3568
BbeI G_GCGC'C
Cuts at: 2032 2280 2032
Size: 248 4350
BbsI GAAGACnn'nnnn_
Cuts at: 644 644
Size: 4598
BbvCI CC'TCA_GC
Cuts at: 1259 1259
Size: 4598
BciVI GTATCCnnnnn_n'
Cuts at: 2496 3257 4598 2496
Size: 761 1341 2496
Fragments arranged by size:
2496 1341 761
BclI T'GATC_A
Cuts at: 2118 2118
Size: 4598
BglII A'GATC_T
Cuts at: 773 2113 4150 773
Size: 1340 2037 1221
Fragments arranged by size:
2037 1340 1221
BlpI GC'TnA_GC
Cuts at: 1710 1710
Size: 4598
Bme1580I G_kGCm'C
Cuts at: 2223 2316 3372 2223
Size: 93 1056 3449
Fragments arranged by size:
3449 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1810 1891 2226 4370 1810
Size: 81 335 2144 2038
Fragments arranged by size:
2144 2038 335 81
BmtI G_CTAG'C
Cuts at: 1249 1249
Size: 4598
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 4598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 891 346
Size: 545 4053
Bpu10I CC'TnA_GC
Cuts at: 1259 1259
Size: 4598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1774 2653 3145 3443 1774
Size: 879 492 298 2929
Fragments arranged by size:
2929 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4302 4302
Size: 4598
BsaAI yAC'GTr
Cuts at: 1951 1951
Size: 4598
BsaBI GATnn'nnATC
Cuts at: 6 1529 2112 4149 6
Size: 1523 583 2037 455
Fragments arranged by size:
2037 1523 583 455
BsaHI Gr'CG_yC
Cuts at: 2029 2277 2979 4475 2029
Size: 248 702 1496 2152
Fragments arranged by size:
2152 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1787 2308 2986 3260 3407 3818 1787
Size: 521 678 274 147 411 2567
Fragments arranged by size:
2567 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 904 997 1237 904
Size: 93 240 4265
Fragments arranged by size:
4265 240 93
BseYI C'CCAG_C
Cuts at: 2023 2781 3358 3854 2023
Size: 758 577 496 2767
Fragments arranged by size:
2767 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4598
BsiEI CG_ry'CG
Cuts at: 104 2140 2186 3394 104
Size: 2036 46 1208 1308
Fragments arranged by size:
2036 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1612 2390 2580 3372 1612
Size: 778 190 792 2838
Fragments arranged by size:
2838 792 778 190
BsmI GAATG_Cn'
Cuts at: 633 828 1011 1290 633
Size: 195 183 279 3941
Fragments arranged by size:
3941 279 195 183
Bsp1286I G_dGCh'C
Cuts at: 1612 2223 2316 2390 2580 2642 3372 1612
Size: 611 93 74 190 62 730 2838
Fragments arranged by size:
2838 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3818 3818
Size: 4598
BspHI T'CATG_A
Cuts at: 749 3774 749
Size: 3025 1573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1648 1862 2164 2545 1648
Size: 214 302 381 3701
Fragments arranged by size:
3701 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2890 2944 4428 4593 50
Size: 2840 54 1484 165 55
Fragments arranged by size:
2840 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 833 2510 833
Size: 1677 2921
BsrFI r'CCGG_y
Cuts at: 2596 2777 2986 2596
Size: 181 209 4208
Fragments arranged by size:
4208 209 181
BssSI C'ACGA_G
Cuts at: 2869 3227 2869
Size: 358 4240
BstAPI GCAn_nnn'nTGC
Cuts at: 1975 1975
Size: 4598
BstBI TT'CG_AA
Cuts at: 2959 2959
Size: 4598
BstYI r'GATC_y
Cuts at: 1 773 1623 2090 2113 2448 2694 3706 3792
Size: 772 850 467 23 335 246 1012 86
Cuts at: 3792 3804 4150 1
Size: 12 346 449
Fragments arranged by size:
1012 850 772 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4598
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2548 2729 2892 2548
Size: 181 163 4254
Fragments arranged by size:
4254 181 163
BtsI GCAGTG_nn'
Cuts at: 1841 1841
Size: 4598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1362 1397 1362
Size: 35 4563
DraI TTT'AAA
Cuts at: 1512 1597 1512
Size: 85 4513
DrdI GACnn_nn'nnGTC
Cuts at: 2304 3162 2304
Size: 858 3740
EaeI y'GGCC_r
Cuts at: 2183 2357 2748 2775 4120 2183
Size: 174 391 27 1345 2661
Fragments arranged by size:
2661 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2183 2183
Size: 4598
EarI CTCTTCn'nnn_
Cuts at: 311 617 839 1214 1340 1385 1538 1580 2621
Size: 306 222 375 126 45 153 42 1041
Cuts at: 2621 2831 311
Size: 210 2078
Fragments arranged by size:
2078 1041 375 306 222 210 153 126
45 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2806 3116 3262 4408 2806
Size: 310 146 1146 2996
Fragments arranged by size:
2996 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1610 1610
Size: 4598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 891 985 2423 2855 3602 3870 346
Size: 545 94 1438 432 747 268 1074
Fragments arranged by size:
1438 1074 747 545 432 268 94
EcoO109I rG'GnC_Cy
Cuts at: 1737 1737
Size: 4598
EcoRI G'AATT_C
Cuts at: 1602 1602
Size: 4598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2617 2617
Size: 4598
FspI TGC'GCA
Cuts at: 2379 2379
Size: 4598
HaeII r_GCGC'y
Cuts at: 2032 2280 3302 4281 2032
Size: 248 1022 979 2349
Fragments arranged by size:
2349 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2440 2470 2472 2502 2440
Size: 30 2 30 4536
Fragments arranged by size:
4536 30 30 2
HincII GTy'rAC
Cuts at: 210 1013 1637 210
Size: 803 624 3171
Fragments arranged by size:
3171 803 624
HindIII A'AGCT_T
Cuts at: 719 719
Size: 4598
KasI G'GCGC_C
Cuts at: 2028 2276 2028
Size: 248 4350
KpnI G_GTAC'C
Cuts at: 1618 1618
Size: 4598
MluI A'CGCG_T
Cuts at: 4031 4031
Size: 4598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 287 773 3269 3453 287
Size: 486 2496 184 1432
Fragments arranged by size:
2496 1432 486 184
MscI TGG'CCA
Cuts at: 2359 2359
Size: 4598
MslI CAynn'nnrTG
Cuts at: 2587 2714 2587
Size: 127 4471
MspA1I CmG'CkG
Cuts at: 732 1311 1709 2023 2383 3396 3641 732
Size: 579 398 314 360 1013 245 1689
Fragments arranged by size:
1689 1013 579 398 360 314 245
NaeI GCC'GGC
Cuts at: 2779 2779
Size: 4598
NarI GG'CG_CC
Cuts at: 2029 2277 2029
Size: 248 4350
NcoI C'CATG_G
Cuts at: 107 107
Size: 4598
NgoMIV G'CCGG_C
Cuts at: 2777 2777
Size: 4598
NheI G'CTAG_C
Cuts at: 1245 1245
Size: 4598
NspI r_CATG'y
Cuts at: 190 936 1474 1651 2151 3058 190
Size: 746 538 177 500 907 1730
Fragments arranged by size:
1730 907 746 538 500 177
PciI A'CATG_T
Cuts at: 186 3054 186
Size: 2868 1730
PmeI GTTT'AAAC
Cuts at: 1597 1597
Size: 4598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 350 382 3737 3769 350
Size: 32 3355 32 1179
Fragments arranged by size:
3355 1179 32 32
PstI C_TGCA'G
Cuts at: 636 1645 2330 636
Size: 1009 685 2904
Fragments arranged by size:
2904 1009 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4598
PvuII CAG'CTG
Cuts at: 732 1311 2023 2383 732
Size: 579 712 360 2947
Fragments arranged by size:
2947 712 579 360
RsrII CG'GwC_CG
Cuts at: 2793 2793
Size: 4598
SacI G_AGCT'C
Cuts at: 1612 1612
Size: 4598
SalI G'TCGA_C
Cuts at: 1635 1635
Size: 4598
SapI GCTCTTCn'nnn_
Cuts at: 1340 2621 2831 1340
Size: 1281 210 3107
Fragments arranged by size:
3107 1281 210
SbfI CC_TGCA'GG
Cuts at: 1645 1645
Size: 4598
ScaI AGT'ACT
Cuts at: 667 4115 667
Size: 3448 1150
SfcI C'TryA_G
Cuts at: 33 135 632 994 1201 1641 2326 3319 3510
Size: 102 497 362 207 440 685 993 191
Cuts at: 3510 33
Size: 1121
Fragments arranged by size:
1121 993 685 497 440 362 207 191
102
SfoI GGC'GCC
Cuts at: 2030 2278 2030
Size: 248 4350
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
SmaI CCC'GGG
Cuts at: 1620 1620
Size: 4598
SmlI C'TyrA_G
Cuts at: 1753 2668 3160 3422 1753
Size: 915 492 262 2929
Fragments arranged by size:
2929 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4598
SphI G_CATG'C
Cuts at: 1474 1651 2151 1474
Size: 177 500 3921
Fragments arranged by size:
3921 500 177
StyI C'CwwG_G
Cuts at: 107 1732 2086 107
Size: 1625 354 2619
Fragments arranged by size:
2619 1625 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2181 2314 2773 2982 2181
Size: 133 459 209 3797
Fragments arranged by size:
3797 459 209 133
TatI w'GTAC_w
Cuts at: 665 4113 665
Size: 3448 1150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4115 4147 4115
Size: 32 4566
Tth111I GACn'n_nGTC
Cuts at: 2395 2395
Size: 4598
XbaI T'CTAG_A
Cuts at: 1629 1629
Size: 4598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 591 2093 591
Size: 1502 3096
XmaI C'CCGG_G
Cuts at: 1618 1618
Size: 4598
XmnI GAAnn'nnTTC
Cuts at: 848 848
Size: 4598
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI