(Circular) (Six-base) MAPSORT of: pF1KB5878.seq Check: 5777 from: 1 to: 4598 pF1KB5878 4598 bp With 182 enzymes: SgfI * April 24, 2009 13:05 .. AccI GT'mk_AC Cuts at: 1636 1636 Size: 4598 Acc65I G'GTAC_C Cuts at: 1614 1614 Size: 4598 AclI AA'CG_TT Cuts at: 4438 4438 Size: 4598 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 985 2423 2855 3602 3870 985 Size: 1438 432 747 268 1713 Fragments arranged by size: 1713 1438 747 432 268 AflIII A'CryG_T Cuts at: 186 1950 3054 4031 186 Size: 1764 1104 977 753 Fragments arranged by size: 1764 1104 977 753 AgeI A'CCGG_T Cuts at: 2986 2986 Size: 4598 AlwNI CAG_nnn'CTG Cuts at: 1903 3470 1903 Size: 1567 3031 ApaLI G'TGCA_C Cuts at: 3368 3368 Size: 4598 ApoI r'AATT_y Cuts at: 126 1252 1577 1602 3951 4207 126 Size: 1126 325 25 2349 256 517 Fragments arranged by size: 2349 1126 517 325 256 25 AseI AT'TA_AT Cuts at: 20 1279 1427 20 Size: 1259 148 3191 Fragments arranged by size: 3191 1259 148 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4598 AvaI C'yCGr_G Cuts at: 1618 1618 Size: 4598 BamHI G'GATC_C Cuts at: 1623 1623 Size: 4598 BanI G'GyrC_C Cuts at: 1614 2028 2276 2311 1614 Size: 414 248 35 3901 Fragments arranged by size: 3901 414 248 35 BanII G_rGCy'C Cuts at: 1612 2642 1612 Size: 1030 3568 BbeI G_GCGC'C Cuts at: 2032 2280 2032 Size: 248 4350 BbsI GAAGACnn'nnnn_ Cuts at: 644 644 Size: 4598 BbvCI CC'TCA_GC Cuts at: 1259 1259 Size: 4598 BciVI GTATCCnnnnn_n' Cuts at: 2496 3257 4598 2496 Size: 761 1341 2496 Fragments arranged by size: 2496 1341 761 BclI T'GATC_A Cuts at: 2118 2118 Size: 4598 BglII A'GATC_T Cuts at: 773 2113 4150 773 Size: 1340 2037 1221 Fragments arranged by size: 2037 1340 1221 BlpI GC'TnA_GC Cuts at: 1710 1710 Size: 4598 Bme1580I G_kGCm'C Cuts at: 2223 2316 3372 2223 Size: 93 1056 3449 Fragments arranged by size: 3449 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1810 1891 2226 4370 1810 Size: 81 335 2144 2038 Fragments arranged by size: 2144 2038 335 81 BmtI G_CTAG'C Cuts at: 1249 1249 Size: 4598 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2965 2965 Size: 4598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 346 891 346 Size: 545 4053 Bpu10I CC'TnA_GC Cuts at: 1259 1259 Size: 4598 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1774 2653 3145 3443 1774 Size: 879 492 298 2929 Fragments arranged by size: 2929 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4302 4302 Size: 4598 BsaAI yAC'GTr Cuts at: 1951 1951 Size: 4598 BsaBI GATnn'nnATC Cuts at: 6 1529 2112 4149 6 Size: 1523 583 2037 455 Fragments arranged by size: 2037 1523 583 455 BsaHI Gr'CG_yC Cuts at: 2029 2277 2979 4475 2029 Size: 248 702 1496 2152 Fragments arranged by size: 2152 1496 702 248 BsaWI w'CCGG_w Cuts at: 1787 2308 2986 3260 3407 3818 1787 Size: 521 678 274 147 411 2567 Fragments arranged by size: 2567 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 904 997 1237 904 Size: 93 240 4265 Fragments arranged by size: 4265 240 93 BseYI C'CCAG_C Cuts at: 2023 2781 3358 3854 2023 Size: 758 577 496 2767 Fragments arranged by size: 2767 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 256 256 Size: 4598 BsiEI CG_ry'CG Cuts at: 104 2140 2186 3394 104 Size: 2036 46 1208 1308 Fragments arranged by size: 2036 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1612 2390 2580 3372 1612 Size: 778 190 792 2838 Fragments arranged by size: 2838 792 778 190 BsmI GAATG_Cn' Cuts at: 633 828 1011 1290 633 Size: 195 183 279 3941 Fragments arranged by size: 3941 279 195 183 Bsp1286I G_dGCh'C Cuts at: 1612 2223 2316 2390 2580 2642 3372 1612 Size: 611 93 74 190 62 730 2838 Fragments arranged by size: 2838 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3818 3818 Size: 4598 BspHI T'CATG_A Cuts at: 749 3774 749 Size: 3025 1573 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1648 1862 2164 2545 1648 Size: 214 302 381 3701 Fragments arranged by size: 3701 381 302 214 BsrBI CCG'CTC Cuts at: 50 2890 2944 4428 4593 50 Size: 2840 54 1484 165 55 Fragments arranged by size: 2840 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 833 2510 833 Size: 1677 2921 BsrFI r'CCGG_y Cuts at: 2596 2777 2986 2596 Size: 181 209 4208 Fragments arranged by size: 4208 209 181 BssSI C'ACGA_G Cuts at: 2869 3227 2869 Size: 358 4240 BstAPI GCAn_nnn'nTGC Cuts at: 1975 1975 Size: 4598 BstBI TT'CG_AA Cuts at: 2959 2959 Size: 4598 BstYI r'GATC_y Cuts at: 1 773 1623 2090 2113 2448 2694 3706 3792 Size: 772 850 467 23 335 246 1012 86 Cuts at: 3792 3804 4150 1 Size: 12 346 449 Fragments arranged by size: 1012 850 772 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4598 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2548 2729 2892 2548 Size: 181 163 4254 Fragments arranged by size: 4254 181 163 BtsI GCAGTG_nn' Cuts at: 1841 1841 Size: 4598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1362 1397 1362 Size: 35 4563 DraI TTT'AAA Cuts at: 1512 1597 1512 Size: 85 4513 DrdI GACnn_nn'nnGTC Cuts at: 2304 3162 2304 Size: 858 3740 EaeI y'GGCC_r Cuts at: 2183 2357 2748 2775 4120 2183 Size: 174 391 27 1345 2661 Fragments arranged by size: 2661 1345 391 174 27 EagI C'GGCC_G Cuts at: 2183 2183 Size: 4598 EarI CTCTTCn'nnn_ Cuts at: 311 617 839 1214 1340 1385 1538 1580 2621 Size: 306 222 375 126 45 153 42 1041 Cuts at: 2621 2831 311 Size: 210 2078 Fragments arranged by size: 2078 1041 375 306 222 210 153 126 45 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2806 3116 3262 4408 2806 Size: 310 146 1146 2996 Fragments arranged by size: 2996 1146 310 146 EcoICRI GAG'CTC Cuts at: 1610 1610 Size: 4598 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 346 891 985 2423 2855 3602 3870 346 Size: 545 94 1438 432 747 268 1074 Fragments arranged by size: 1438 1074 747 545 432 268 94 EcoO109I rG'GnC_Cy Cuts at: 1737 1737 Size: 4598 EcoRI G'AATT_C Cuts at: 1602 1602 Size: 4598 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2617 2617 Size: 4598 FspI TGC'GCA Cuts at: 2379 2379 Size: 4598 HaeII r_GCGC'y Cuts at: 2032 2280 3302 4281 2032 Size: 248 1022 979 2349 Fragments arranged by size: 2349 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2440 2470 2472 2502 2440 Size: 30 2 30 4536 Fragments arranged by size: 4536 30 30 2 HincII GTy'rAC Cuts at: 210 1013 1637 210 Size: 803 624 3171 Fragments arranged by size: 3171 803 624 HindIII A'AGCT_T Cuts at: 719 719 Size: 4598 KasI G'GCGC_C Cuts at: 2028 2276 2028 Size: 248 4350 KpnI G_GTAC'C Cuts at: 1618 1618 Size: 4598 MluI A'CGCG_T Cuts at: 4031 4031 Size: 4598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 287 773 3269 3453 287 Size: 486 2496 184 1432 Fragments arranged by size: 2496 1432 486 184 MscI TGG'CCA Cuts at: 2359 2359 Size: 4598 MslI CAynn'nnrTG Cuts at: 2587 2714 2587 Size: 127 4471 MspA1I CmG'CkG Cuts at: 732 1311 1709 2023 2383 3396 3641 732 Size: 579 398 314 360 1013 245 1689 Fragments arranged by size: 1689 1013 579 398 360 314 245 NaeI GCC'GGC Cuts at: 2779 2779 Size: 4598 NarI GG'CG_CC Cuts at: 2029 2277 2029 Size: 248 4350 NcoI C'CATG_G Cuts at: 107 107 Size: 4598 NgoMIV G'CCGG_C Cuts at: 2777 2777 Size: 4598 NheI G'CTAG_C Cuts at: 1245 1245 Size: 4598 NspI r_CATG'y Cuts at: 190 936 1474 1651 2151 3058 190 Size: 746 538 177 500 907 1730 Fragments arranged by size: 1730 907 746 538 500 177 PciI A'CATG_T Cuts at: 186 3054 186 Size: 2868 1730 PmeI GTTT'AAAC Cuts at: 1597 1597 Size: 4598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 350 382 3737 3769 350 Size: 32 3355 32 1179 Fragments arranged by size: 3355 1179 32 32 PstI C_TGCA'G Cuts at: 636 1645 2330 636 Size: 1009 685 2904 Fragments arranged by size: 2904 1009 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4598 PvuII CAG'CTG Cuts at: 732 1311 2023 2383 732 Size: 579 712 360 2947 Fragments arranged by size: 2947 712 579 360 RsrII CG'GwC_CG Cuts at: 2793 2793 Size: 4598 SacI G_AGCT'C Cuts at: 1612 1612 Size: 4598 SalI G'TCGA_C Cuts at: 1635 1635 Size: 4598 SapI GCTCTTCn'nnn_ Cuts at: 1340 2621 2831 1340 Size: 1281 210 3107 Fragments arranged by size: 3107 1281 210 SbfI CC_TGCA'GG Cuts at: 1645 1645 Size: 4598 ScaI AGT'ACT Cuts at: 667 4115 667 Size: 3448 1150 SfcI C'TryA_G Cuts at: 33 135 632 994 1201 1641 2326 3319 3510 Size: 102 497 362 207 440 685 993 191 Cuts at: 3510 33 Size: 1121 Fragments arranged by size: 1121 993 685 497 440 362 207 191 102 SfoI GGC'GCC Cuts at: 2030 2278 2030 Size: 248 4350 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4598 SmaI CCC'GGG Cuts at: 1620 1620 Size: 4598 SmlI C'TyrA_G Cuts at: 1753 2668 3160 3422 1753 Size: 915 492 262 2929 Fragments arranged by size: 2929 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4598 SphI G_CATG'C Cuts at: 1474 1651 2151 1474 Size: 177 500 3921 Fragments arranged by size: 3921 500 177 StyI C'CwwG_G Cuts at: 107 1732 2086 107 Size: 1625 354 2619 Fragments arranged by size: 2619 1625 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2181 2314 2773 2982 2181 Size: 133 459 209 3797 Fragments arranged by size: 3797 459 209 133 TatI w'GTAC_w Cuts at: 665 4113 665 Size: 3448 1150 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4115 4147 4115 Size: 32 4566 Tth111I GACn'n_nGTC Cuts at: 2395 2395 Size: 4598 XbaI T'CTAG_A Cuts at: 1629 1629 Size: 4598 XcmI CCAnnnn_n'nnnnTGG Cuts at: 591 2093 591 Size: 1502 3096 XmaI C'CCGG_G Cuts at: 1618 1618 Size: 4598 XmnI GAAnn'nnTTC Cuts at: 848 848 Size: 4598 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI