Restriction map for
pF1KB5887
(Circular) (Six-base) MAPSORT of: pF1KB5887.seq Check: 9894 from: 1 to: 4604
pF1KB5887 4604 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 421 1642 421
Size: 1221 3383
Acc65I G'GTAC_C
Cuts at: 1620 1620
Size: 4604
AclI AA'CG_TT
Cuts at: 4444 4444
Size: 4604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1439 1553 2429 2861 3608 3876 1439
Size: 114 876 432 747 268 2167
Fragments arranged by size:
2167 876 747 432 268 114
AflIII A'CryG_T
Cuts at: 1956 3060 4037 1956
Size: 1104 977 2523
Fragments arranged by size:
2523 1104 977
AgeI A'CCGG_T
Cuts at: 2992 2992
Size: 4604
AhdI GACnn_n'nnGTC
Cuts at: 1510 1510
Size: 4604
AlwNI CAG_nnn'CTG
Cuts at: 1909 3476 1909
Size: 1567 3037
ApaLI G'TGCA_C
Cuts at: 3374 3374
Size: 4604
ApoI r'AATT_y
Cuts at: 172 426 852 861 1295 1586 1608 3957 4213
Size: 254 426 9 434 291 22 2349 256
Cuts at: 4213 172
Size: 563
Fragments arranged by size:
2349 563 434 426 291 256 254 22
9
AseI AT'TA_AT
Cuts at: 20 20
Size: 4604
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
AvaI C'yCGr_G
Cuts at: 570 1624 570
Size: 1054 3550
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 128 161 128
Size: 33 4571
BamHI G'GATC_C
Cuts at: 1629 1629
Size: 4604
BanI G'GyrC_C
Cuts at: 1433 1620 2034 2282 2317 1433
Size: 187 414 248 35 3720
Fragments arranged by size:
3720 414 248 187 35
BanII G_rGCy'C
Cuts at: 1618 2648 1618
Size: 1030 3574
BbeI G_GCGC'C
Cuts at: 1437 2038 2286 1437
Size: 601 248 3755
Fragments arranged by size:
3755 601 248
BbsI GAAGACnn'nnnn_
Cuts at: 357 1055 357
Size: 698 3906
BciVI GTATCCnnnnn_n'
Cuts at: 375 445 1088 2502 3263 4604 375
Size: 70 643 1414 761 1341 375
Fragments arranged by size:
1414 1341 761 643 375 70
BclI T'GATC_A
Cuts at: 2124 2124
Size: 4604
BglII A'GATC_T
Cuts at: 1344 2119 4156 1344
Size: 775 2037 1792
Fragments arranged by size:
2037 1792 775
BlpI GC'TnA_GC
Cuts at: 1716 1716
Size: 4604
Bme1580I G_kGCm'C
Cuts at: 2229 2322 3378 2229
Size: 93 1056 3455
Fragments arranged by size:
3455 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 545 1037 1816 1897 2232 4376 545
Size: 492 779 81 335 2144 773
Fragments arranged by size:
2144 779 773 492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4604
Bpu10I CC'TnA_GC
Cuts at: 1429 1429
Size: 4604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 1348 1780 2659 3151 3449 1171
Size: 177 432 879 492 298 2326
Fragments arranged by size:
2326 879 492 432 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 4308 4308
Size: 4604
BsaAI yAC'GTr
Cuts at: 1957 1957
Size: 4604
BsaBI GATnn'nnATC
Cuts at: 6 2118 4155 6
Size: 2112 2037 455
Fragments arranged by size:
2112 2037 455
BsaHI Gr'CG_yC
Cuts at: 1434 2035 2283 2985 4481 1434
Size: 601 248 702 1496 1557
Fragments arranged by size:
1557 1496 702 601 248
BsaWI w'CCGG_w
Cuts at: 1793 2314 2992 3266 3413 3824 1793
Size: 521 678 274 147 411 2573
Fragments arranged by size:
2573 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 272 302 272
Size: 30 4574
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1034 1168 1459 1034
Size: 134 291 4179
Fragments arranged by size:
4179 291 134
BseYI C'CCAG_C
Cuts at: 2029 2787 3364 3860 2029
Size: 758 577 496 2773
Fragments arranged by size:
2773 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 721
Size: 4604
BsiEI CG_ry'CG
Cuts at: 104 2146 2192 3400 104
Size: 2042 46 1208 1308
Fragments arranged by size:
2042 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1618 2396 2586 3378 1618
Size: 778 190 792 2844
Fragments arranged by size:
2844 792 778 190
BsmI GAATG_Cn'
Cuts at: 1359 1359
Size: 4604
Bsp1286I G_dGCh'C
Cuts at: 1618 2229 2322 2396 2586 2648 3378 1618
Size: 611 93 74 190 62 730 2844
Fragments arranged by size:
2844 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3824 3824
Size: 4604
BspHI T'CATG_A
Cuts at: 3780 3780
Size: 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 156 531 1654 1868 2170 2551 156
Size: 375 1123 214 302 381 2209
Fragments arranged by size:
2209 1123 381 375 302 214
BsrBI CCG'CTC
Cuts at: 50 2896 2950 4434 4599 50
Size: 2846 54 1484 165 55
Fragments arranged by size:
2846 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2516 2516
Size: 4604
BsrFI r'CCGG_y
Cuts at: 2602 2783 2992 2602
Size: 181 209 4214
Fragments arranged by size:
4214 209 181
BssSI C'ACGA_G
Cuts at: 251 2875 3233 251
Size: 2624 358 1622
Fragments arranged by size:
2624 1622 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1981 1981
Size: 4604
BstBI TT'CG_AA
Cuts at: 819 2965 819
Size: 2146 2458
BstEII G'GTnAC_C
Cuts at: 691 691
Size: 4604
BstYI r'GATC_y
Cuts at: 1 130 911 1344 1629 2096 2119 2454 2700
Size: 129 781 433 285 467 23 335 246
Cuts at: 2700 3712 3798 3810 4156 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 781 467 449 433 346 335 285
246 129 86 23 12
BtgI C'CryG_G
Cuts at: 890 890
Size: 4604
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2554 2735 2898 2554
Size: 181 163 4260
Fragments arranged by size:
4260 181 163
BtsI GCAGTG_nn'
Cuts at: 709 1847 709
Size: 1138 3466
DraI TTT'AAA
Cuts at: 1603 1603
Size: 4604
DrdI GACnn_nn'nnGTC
Cuts at: 2310 3168 2310
Size: 858 3746
EaeI y'GGCC_r
Cuts at: 625 2189 2363 2754 2781 4126 625
Size: 1564 174 391 27 1345 1103
Fragments arranged by size:
1564 1345 1103 391 174 27
EagI C'GGCC_G
Cuts at: 2189 2189
Size: 4604
EarI CTCTTCn'nnn_
Cuts at: 176 1415 2627 2837 176
Size: 1239 1212 210 1943
Fragments arranged by size:
1943 1239 1212 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2812 3122 3268 4414 2812
Size: 310 146 1146 3002
Fragments arranged by size:
3002 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1616 1616
Size: 4604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1439 1553 2429 2861 3608 3876 1439
Size: 114 876 432 747 268 2167
Fragments arranged by size:
2167 876 747 432 268 114
EcoNI CCTnn'n_nnAGG
Cuts at: 495 1441 495
Size: 946 3658
EcoO109I rG'GnC_Cy
Cuts at: 1743 1743
Size: 4604
EcoRI G'AATT_C
Cuts at: 1608 1608
Size: 4604
EcoRV GAT'ATC
Cuts at: 1304 1304
Size: 4604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1409 2623 1409
Size: 1214 3390
FspI TGC'GCA
Cuts at: 2385 2385
Size: 4604
HaeII r_GCGC'y
Cuts at: 1437 2038 2286 3308 4287 1437
Size: 601 248 1022 979 1754
Fragments arranged by size:
1754 1022 979 601 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 1496 1528 2446 2476 2478 2508 487
Size: 32 977 32 918 30 2 30 2583
Fragments arranged by size:
2583 977 918 32 32 30 30 2
HincII GTy'rAC
Cuts at: 945 1542 1643 945
Size: 597 101 3906
Fragments arranged by size:
3906 597 101
HindIII A'AGCT_T
Cuts at: 1450 1450
Size: 4604
HpaI GTT'AAC
Cuts at: 945 945
Size: 4604
KasI G'GCGC_C
Cuts at: 1433 2034 2282 1433
Size: 601 248 3755
Fragments arranged by size:
3755 601 248
KpnI G_GTAC'C
Cuts at: 1624 1624
Size: 4604
MluI A'CGCG_T
Cuts at: 4037 4037
Size: 4604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 327 3275 3459 327
Size: 2948 184 1472
Fragments arranged by size:
2948 1472 184
MscI TGG'CCA
Cuts at: 627 2365 627
Size: 1738 2866
MslI CAynn'nnrTG
Cuts at: 2593 2720 2593
Size: 127 4477
MspA1I CmG'CkG
Cuts at: 313 1715 2029 2389 3402 3647 313
Size: 1402 314 360 1013 245 1270
Fragments arranged by size:
1402 1270 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2785 2785
Size: 4604
NarI GG'CG_CC
Cuts at: 1434 2035 2283 1434
Size: 601 248 3755
Fragments arranged by size:
3755 601 248
NcoI C'CATG_G
Cuts at: 890 890
Size: 4604
NgoMIV G'CCGG_C
Cuts at: 2783 2783
Size: 4604
NspI r_CATG'y
Cuts at: 517 1069 1657 2157 3064 517
Size: 552 588 500 907 2057
Fragments arranged by size:
2057 907 588 552 500
PciI A'CATG_T
Cuts at: 3060 3060
Size: 4604
PmeI GTTT'AAAC
Cuts at: 1603 1603
Size: 4604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 4572
PshAI GACnn'nnGTC
Cuts at: 1547 1547
Size: 4604
PstI C_TGCA'G
Cuts at: 153 1651 2336 153
Size: 1498 685 2421
Fragments arranged by size:
2421 1498 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4604
PvuII CAG'CTG
Cuts at: 313 2029 2389 313
Size: 1716 360 2528
Fragments arranged by size:
2528 1716 360
RsrII CG'GwC_CG
Cuts at: 2799 2799
Size: 4604
SacI G_AGCT'C
Cuts at: 1618 1618
Size: 4604
SalI G'TCGA_C
Cuts at: 1641 1641
Size: 4604
SapI GCTCTTCn'nnn_
Cuts at: 1415 2627 2837 1415
Size: 1212 210 3182
Fragments arranged by size:
3182 1212 210
SbfI CC_TGCA'GG
Cuts at: 1651 1651
Size: 4604
ScaI AGT'ACT
Cuts at: 1416 4121 1416
Size: 2705 1899
SfcI C'TryA_G
Cuts at: 33 149 314 1647 2332 3325 3516 33
Size: 116 165 1333 685 993 191 1121
Fragments arranged by size:
1333 1121 993 685 191 165 116
SfoI GGC'GCC
Cuts at: 1435 2036 2284 1435
Size: 601 248 3755
Fragments arranged by size:
3755 601 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
SmaI CCC'GGG
Cuts at: 1626 1626
Size: 4604
SmlI C'TyrA_G
Cuts at: 1150 1327 1759 2674 3166 3428 1150
Size: 177 432 915 492 262 2326
Fragments arranged by size:
2326 915 492 432 262 177
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4604
SphI G_CATG'C
Cuts at: 1657 2157 1657
Size: 500 4104
SspI AAT'ATT
Cuts at: 816 1131 1479 816
Size: 315 348 3941
Fragments arranged by size:
3941 348 315
StyI C'CwwG_G
Cuts at: 890 1054 1738 2092 890
Size: 164 684 354 3402
Fragments arranged by size:
3402 684 354 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2187 2320 2779 2988 2187
Size: 133 459 209 3803
Fragments arranged by size:
3803 459 209 133
TatI w'GTAC_w
Cuts at: 449 1414 4119 449
Size: 965 2705 934
Fragments arranged by size:
2705 965 934
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 272 304 1029 1061 4121 4153 272
Size: 32 725 32 3060 32 723
Fragments arranged by size:
3060 725 723 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2401 2401
Size: 4604
XbaI T'CTAG_A
Cuts at: 1635 1635
Size: 4604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2099 2099
Size: 4604
XmaI C'CCGG_G
Cuts at: 1624 1624
Size: 4604
XmnI GAAnn'nnTTC
Cuts at: 1295 1295
Size: 4604
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI