Restriction map for
pF1KB5888
(Circular) (Six-base) MAPSORT of: pF1KB5888.seq Check: 8172 from: 1 to: 4676
pF1KB5888 4676 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 887 1714 887
Size: 827 3849
Acc65I G'GTAC_C
Cuts at: 1618 1692 1618
Size: 74 4602
AclI AA'CG_TT
Cuts at: 4516 4516
Size: 4676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1622 2501 2933 3680 3948 1622
Size: 879 432 747 268 2350
Fragments arranged by size:
2350 879 747 432 268
AflIII A'CryG_T
Cuts at: 2028 3132 4109 2028
Size: 1104 977 2595
Fragments arranged by size:
2595 1104 977
AgeI A'CCGG_T
Cuts at: 3064 3064
Size: 4676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 593 593
Size: 4676
AlwNI CAG_nnn'CTG
Cuts at: 1981 3548 1981
Size: 1567 3109
ApaLI G'TGCA_C
Cuts at: 3446 3446
Size: 4676
ApoI r'AATT_y
Cuts at: 1660 1680 4029 4285 1660
Size: 20 2349 256 2051
Fragments arranged by size:
2349 2051 256 20
AseI AT'TA_AT
Cuts at: 20 1213 20
Size: 1193 3483
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
AvaI C'yCGr_G
Cuts at: 1696 1696
Size: 4676
BamHI G'GATC_C
Cuts at: 801 1701 801
Size: 900 3776
BanI G'GyrC_C
Cuts at: 1048 1618 1692 2106 2354 2389 1048
Size: 570 74 414 248 35 3335
Fragments arranged by size:
3335 570 414 248 74 35
BanII G_rGCy'C
Cuts at: 903 1690 2720 903
Size: 787 1030 2859
Fragments arranged by size:
2859 1030 787
BbeI G_GCGC'C
Cuts at: 2110 2358 2110
Size: 248 4428
BbsI GAAGACnn'nnnn_
Cuts at: 192 1526 192
Size: 1334 3342
BciVI GTATCCnnnnn_n'
Cuts at: 2574 3335 4676 2574
Size: 761 1341 2574
Fragments arranged by size:
2574 1341 761
BclI T'GATC_A
Cuts at: 2196 2196
Size: 4676
BglII A'GATC_T
Cuts at: 2191 4228 2191
Size: 2037 2639
BlpI GC'TnA_GC
Cuts at: 1788 1788
Size: 4676
Bme1580I G_kGCm'C
Cuts at: 968 980 2301 2394 3450 968
Size: 12 1321 93 1056 2194
Fragments arranged by size:
2194 1321 1056 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 355 709 1888 1969 2304 4448 355
Size: 354 1179 81 335 2144 583
Fragments arranged by size:
2144 1179 583 354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3043 3043
Size: 4676
Bpu10I CC'TnA_GC
Cuts at: 1241 1241
Size: 4676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1367 1852 2731 3223 3521 542
Size: 825 485 879 492 298 1697
Fragments arranged by size:
1697 879 825 492 485 298
BsaI GGTCTCn'nnnn_
Cuts at: 4380 4380
Size: 4676
BsaAI yAC'GTr
Cuts at: 2029 2029
Size: 4676
BsaBI GATnn'nnATC
Cuts at: 6 488 1583 2190 4227 6
Size: 482 1095 607 2037 455
Fragments arranged by size:
2037 1095 607 482 455
BsaHI Gr'CG_yC
Cuts at: 2107 2355 3057 4553 2107
Size: 248 702 1496 2230
Fragments arranged by size:
2230 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1865 2386 3064 3338 3485 3896 1865
Size: 521 678 274 147 411 2645
Fragments arranged by size:
2645 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 121 2101 2859 3436 3932 121
Size: 1980 758 577 496 865
Fragments arranged by size:
1980 865 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 589 533
Size: 56 4620
BsiEI CG_ry'CG
Cuts at: 104 2218 2264 3472 104
Size: 2114 46 1208 1308
Fragments arranged by size:
2114 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1690 2468 2658 3450 1690
Size: 778 190 792 2916
Fragments arranged by size:
2916 792 778 190
BsmI GAATG_Cn'
Cuts at: 348 348
Size: 4676
Bsp1286I G_dGCh'C
Cuts at: 903 968 980 1690 2301 2394 2468 2658 2720
Size: 65 12 710 611 93 74 190 62
Cuts at: 2720 3450 903
Size: 730 2129
Fragments arranged by size:
2129 730 710 611 190 93 74 65
62 12
BspEI T'CCGG_A
Cuts at: 3896 3896
Size: 4676
BspHI T'CATG_A
Cuts at: 3852 3852
Size: 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1726 1940 2242 2623 1726
Size: 214 302 381 3779
Fragments arranged by size:
3779 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2968 3022 4506 4671 50
Size: 2918 54 1484 165 55
Fragments arranged by size:
2918 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2588 2588
Size: 4676
BsrFI r'CCGG_y
Cuts at: 1432 2674 2855 3064 1432
Size: 1242 181 209 3044
Fragments arranged by size:
3044 1242 209 181
BsrGI T'GTAC_A
Cuts at: 1329 1329
Size: 4676
BssSI C'ACGA_G
Cuts at: 2947 3305 2947
Size: 358 4318
BstAPI GCAn_nnn'nTGC
Cuts at: 2053 2053
Size: 4676
BstBI TT'CG_AA
Cuts at: 3037 3037
Size: 4676
BstYI r'GATC_y
Cuts at: 1 801 1584 1701 2168 2191 2526 2772 3784
Size: 800 783 117 467 23 335 246 1012
Cuts at: 3784 3870 3882 4228 1
Size: 86 12 346 449
Fragments arranged by size:
1012 800 783 467 449 346 335 246
117 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4676
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 182 2626 2807 2970 182
Size: 2444 181 163 1888
Fragments arranged by size:
2444 1888 181 163
BtsI GCAGTG_nn'
Cuts at: 1151 1919 1151
Size: 768 3908
DraI TTT'AAA
Cuts at: 306 1675 306
Size: 1369 3307
DrdI GACnn_nn'nnGTC
Cuts at: 2382 3240 2382
Size: 858 3818
EaeI y'GGCC_r
Cuts at: 110 1430 2261 2435 2826 2853 4198 110
Size: 1320 831 174 391 27 1345 588
Fragments arranged by size:
1345 1320 831 588 391 174 27
EagI C'GGCC_G
Cuts at: 2261 2261
Size: 4676
EarI CTCTTCn'nnn_
Cuts at: 722 2699 2909 722
Size: 1977 210 2489
Fragments arranged by size:
2489 1977 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2884 3194 3340 4486 2884
Size: 310 146 1146 3074
Fragments arranged by size:
3074 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1688 1688
Size: 4676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1622 2501 2933 3680 3948 1622
Size: 879 432 747 268 2350
Fragments arranged by size:
2350 879 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1815 1815
Size: 4676
EcoRI G'AATT_C
Cuts at: 1680 1680
Size: 4676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1266 2695 1266
Size: 1429 3247
FspI TGC'GCA
Cuts at: 2457 2457
Size: 4676
HaeII r_GCGC'y
Cuts at: 2110 2358 3380 4359 2110
Size: 248 1022 979 2427
Fragments arranged by size:
2427 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 674 706 1075 1107 1215 1247 2518 2548 2550
Size: 32 369 32 108 32 1271 30 2
Cuts at: 2550 2580 674
Size: 30 2770
Fragments arranged by size:
2770 1271 369 108 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1715 1715
Size: 4676
KasI G'GCGC_C
Cuts at: 2106 2354 2106
Size: 248 4428
KpnI G_GTAC'C
Cuts at: 1622 1696 1622
Size: 74 4602
MfeI C'AATT_G
Cuts at: 370 1077 370
Size: 707 3969
MluI A'CGCG_T
Cuts at: 4109 4109
Size: 4676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1184 1660 3347 3531 1184
Size: 476 1687 184 2329
Fragments arranged by size:
2329 1687 476 184
MscI TGG'CCA
Cuts at: 2437 2437
Size: 4676
MslI CAynn'nnrTG
Cuts at: 194 620 1514 2665 2792 194
Size: 426 894 1151 127 2078
Fragments arranged by size:
2078 1151 894 426 127
MspA1I CmG'CkG
Cuts at: 1787 2101 2461 3474 3719 1787
Size: 314 360 1013 245 2744
Fragments arranged by size:
2744 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2857 2857
Size: 4676
NarI GG'CG_CC
Cuts at: 2107 2355 2107
Size: 248 4428
NcoI C'CATG_G
Cuts at: 107 107
Size: 4676
NgoMIV G'CCGG_C
Cuts at: 2855 2855
Size: 4676
NspI r_CATG'y
Cuts at: 1729 2229 3136 1729
Size: 500 907 3269
Fragments arranged by size:
3269 907 500
PciI A'CATG_T
Cuts at: 3132 3132
Size: 4676
PmeI GTTT'AAAC
Cuts at: 1675 1675
Size: 4676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4644
PstI C_TGCA'G
Cuts at: 793 1723 2408 793
Size: 930 685 3061
Fragments arranged by size:
3061 930 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4676
PvuII CAG'CTG
Cuts at: 2101 2461 2101
Size: 360 4316
RsrII CG'GwC_CG
Cuts at: 2871 2871
Size: 4676
SacI G_AGCT'C
Cuts at: 1690 1690
Size: 4676
SalI G'TCGA_C
Cuts at: 1713 1713
Size: 4676
SapI GCTCTTCn'nnn_
Cuts at: 2699 2909 2699
Size: 210 4466
SbfI CC_TGCA'GG
Cuts at: 1723 1723
Size: 4676
ScaI AGT'ACT
Cuts at: 4193 4193
Size: 4676
SfcI C'TryA_G
Cuts at: 33 789 1719 2404 3397 3588 33
Size: 756 930 685 993 191 1121
Fragments arranged by size:
1121 993 930 756 685 191
SfoI GGC'GCC
Cuts at: 2108 2356 2108
Size: 248 4428
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
SmaI CCC'GGG
Cuts at: 1698 1698
Size: 4676
SmlI C'TyrA_G
Cuts at: 557 1382 1831 2746 3238 3500 557
Size: 825 449 915 492 262 1733
Fragments arranged by size:
1733 915 825 492 449 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4676
SphI G_CATG'C
Cuts at: 1729 2229 1729
Size: 500 4176
StyI C'CwwG_G
Cuts at: 107 1810 2164 107
Size: 1703 354 2619
Fragments arranged by size:
2619 1703 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1133 2259 2392 2851 3060 1133
Size: 1126 133 459 209 2749
Fragments arranged by size:
2749 1126 459 209 133
TatI w'GTAC_w
Cuts at: 1329 4191 1329
Size: 2862 1814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1132 1164 4193 4225 1132
Size: 32 3029 32 1583
Fragments arranged by size:
3029 1583 32 32
Tth111I GACn'n_nGTC
Cuts at: 2473 2473
Size: 4676
XbaI T'CTAG_A
Cuts at: 276 474 1401 1707 276
Size: 198 927 306 3245
Fragments arranged by size:
3245 927 306 198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2171 2171
Size: 4676
XmaI C'CCGG_G
Cuts at: 1696 1696
Size: 4676
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BseRI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI