Restriction map for
pF1KB5891
(Circular) (Six-base) MAPSORT of: pF1KB5891.seq Check: 5017 from: 1 to: 4607
pF1KB5891 4607 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1074 1074
Size: 4607
AccI GT'mk_AC
Cuts at: 323 1645 323
Size: 1322 3285
Acc65I G'GTAC_C
Cuts at: 1623 1623
Size: 4607
AclI AA'CG_TT
Cuts at: 4447 4447
Size: 4607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 2432 2864 3611 3879 390
Size: 2042 432 747 268 1118
Fragments arranged by size:
2042 1118 747 432 268
AflIII A'CryG_T
Cuts at: 700 1959 3063 4040 700
Size: 1259 1104 977 1267
Fragments arranged by size:
1267 1259 1104 977
AgeI A'CCGG_T
Cuts at: 2995 2995
Size: 4607
AhdI GACnn_n'nnGTC
Cuts at: 1593 1593
Size: 4607
AleI CACnn'nnGTG
Cuts at: 790 790
Size: 4607
AlwNI CAG_nnn'CTG
Cuts at: 463 868 1225 1912 3479 463
Size: 405 357 687 1567 1591
Fragments arranged by size:
1591 1567 687 405 357
ApaI G_GGCC'C
Cuts at: 1313 1313
Size: 4607
ApaLI G'TGCA_C
Cuts at: 3377 3377
Size: 4607
ApoI r'AATT_y
Cuts at: 550 739 925 1576 1611 3960 4216 550
Size: 189 186 651 35 2349 256 941
Fragments arranged by size:
2349 941 651 256 189 186 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 4607
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
AvaI C'yCGr_G
Cuts at: 296 1048 1627 296
Size: 752 579 3276
Fragments arranged by size:
3276 752 579
BamHI G'GATC_C
Cuts at: 354 1632 354
Size: 1278 3329
BanI G'GyrC_C
Cuts at: 140 1623 2037 2285 2320 140
Size: 1483 414 248 35 2427
Fragments arranged by size:
2427 1483 414 248 35
BanII G_rGCy'C
Cuts at: 269 1313 1621 2651 269
Size: 1044 308 1030 2225
Fragments arranged by size:
2225 1044 1030 308
BbeI G_GCGC'C
Cuts at: 2041 2289 2041
Size: 248 4359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4573
BciVI GTATCCnnnnn_n'
Cuts at: 788 2505 3266 4607 788
Size: 1717 761 1341 788
Fragments arranged by size:
1717 1341 788 761
BclI T'GATC_A
Cuts at: 2127 2127
Size: 4607
BglII A'GATC_T
Cuts at: 2122 4159 2122
Size: 2037 2570
BlpI GC'TnA_GC
Cuts at: 1719 1719
Size: 4607
Bme1580I G_kGCm'C
Cuts at: 1313 2232 2325 3381 1313
Size: 919 93 1056 2539
Fragments arranged by size:
2539 1056 919 93
BmgBI CAC'GTC
Cuts at: 1512 1512
Size: 4607
BmrI ACTGGGnnnn_n'
Cuts at: 451 931 1819 1900 2235 4379 451
Size: 480 888 81 335 2144 679
Fragments arranged by size:
2144 888 679 480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 4607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1121 1121
Size: 4607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1783 2662 3154 3452 1783
Size: 879 492 298 2938
Fragments arranged by size:
2938 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1139 4311 1139
Size: 3172 1435
BsaAI yAC'GTr
Cuts at: 688 703 1960 688
Size: 15 1257 3335
Fragments arranged by size:
3335 1257 15
BsaBI GATnn'nnATC
Cuts at: 6 1099 2121 4158 6
Size: 1093 1022 2037 455
Fragments arranged by size:
2037 1093 1022 455
BsaHI Gr'CG_yC
Cuts at: 1144 2038 2286 2988 4484 1144
Size: 894 248 702 1496 1267
Fragments arranged by size:
1496 1267 894 702 248
BsaWI w'CCGG_w
Cuts at: 1796 2317 2995 3269 3416 3827 1796
Size: 521 678 274 147 411 2576
Fragments arranged by size:
2576 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 485 902 1007 1010 1196 1199 1271 1274 1283
Size: 417 105 3 186 3 72 3 9
Cuts at: 1283 1286 1289 1292 1307 1483 1560 485
Size: 3 3 3 15 176 77 3532
Fragments arranged by size:
3532 417 186 176 105 77 72 15
9 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 225 762 1259 2032 2790 3367 3863 225
Size: 537 497 773 758 577 496 969
Fragments arranged by size:
969 773 758 577 537 497 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1355 1355
Size: 4607
BsiEI CG_ry'CG
Cuts at: 104 327 2149 2195 3403 104
Size: 223 1822 46 1208 1308
Fragments arranged by size:
1822 1308 1208 223 46
BsiHKAI G_wGCw'C
Cuts at: 269 1278 1621 2399 2589 3381 269
Size: 1009 343 778 190 792 1495
Fragments arranged by size:
1495 1009 792 778 343 190
BsmI GAATG_Cn'
Cuts at: 190 190
Size: 4607
Bsp1286I G_dGCh'C
Cuts at: 269 1278 1313 1621 2232 2325 2399 2589 2651
Size: 1009 35 308 611 93 74 190 62
Cuts at: 2651 3381 269
Size: 730 1495
Fragments arranged by size:
1495 1009 730 611 308 190 93 74
62 35
BspEI T'CCGG_A
Cuts at: 3827 3827
Size: 4607
BspHI T'CATG_A
Cuts at: 620 3783 620
Size: 3163 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1074 1433 1657 1871 2173 2554 1074
Size: 359 224 214 302 381 3127
Fragments arranged by size:
3127 381 359 302 224 214
BsrBI CCG'CTC
Cuts at: 50 2899 2953 4437 4602 50
Size: 2849 54 1484 165 55
Fragments arranged by size:
2849 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1512 1535 2519 1512
Size: 23 984 3600
Fragments arranged by size:
3600 984 23
BsrFI r'CCGG_y
Cuts at: 2605 2786 2995 2605
Size: 181 209 4217
Fragments arranged by size:
4217 209 181
BssSI C'ACGA_G
Cuts at: 2878 3236 2878
Size: 358 4249
BstAPI GCAn_nnn'nTGC
Cuts at: 1984 1984
Size: 4607
BstBI TT'CG_AA
Cuts at: 2968 2968
Size: 4607
BstYI r'GATC_y
Cuts at: 1 354 955 979 1632 2099 2122 2457 2703
Size: 353 601 24 653 467 23 335 246
Cuts at: 2703 3715 3801 3813 4159 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 653 601 467 449 353 346 335
246 86 24 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1424 2557 2738 2901 1424
Size: 1133 181 163 3130
Fragments arranged by size:
3130 1133 181 163
BtsI GCAGTG_nn'
Cuts at: 376 1850 376
Size: 1474 3133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 770 805 1235 1270 770
Size: 35 430 35 4107
Fragments arranged by size:
4107 430 35 35
DraI TTT'AAA
Cuts at: 1606 1606
Size: 4607
DrdI GACnn_nn'nnGTC
Cuts at: 2313 3171 2313
Size: 858 3749
EaeI y'GGCC_r
Cuts at: 1341 1514 2192 2366 2757 2784 4129 1341
Size: 173 678 174 391 27 1345 1819
Fragments arranged by size:
1819 1345 678 391 174 173 27
EagI C'GGCC_G
Cuts at: 2192 2192
Size: 4607
EarI CTCTTCn'nnn_
Cuts at: 625 704 2630 2840 625
Size: 79 1926 210 2392
Fragments arranged by size:
2392 1926 210 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 836 1064 1073 2815 3125 3271 4417 836
Size: 228 9 1742 310 146 1146 1026
Fragments arranged by size:
1742 1146 1026 310 228 146 9
EcoICRI GAG'CTC
Cuts at: 267 1619 267
Size: 1352 3255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 1121 2432 2864 3611 3879 390
Size: 731 1311 432 747 268 1118
Fragments arranged by size:
1311 1118 747 731 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1022 1022
Size: 4607
EcoO109I rG'GnC_Cy
Cuts at: 1039 1309 1310 1746 1039
Size: 270 1 436 3900
Fragments arranged by size:
3900 436 270 1
EcoRI G'AATT_C
Cuts at: 925 1611 925
Size: 686 3921
EcoRV GAT'ATC
Cuts at: 618 618
Size: 4607
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1410 2626 1410
Size: 1216 3391
FspI TGC'GCA
Cuts at: 1459 2388 1459
Size: 929 3678
HaeII r_GCGC'y
Cuts at: 2041 2289 3311 4290 2041
Size: 248 1022 979 2358
Fragments arranged by size:
2358 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 433 465 1555 1579 1587 1611 2449 2479 2481
Size: 32 1090 24 8 24 838 30 2
Cuts at: 2481 2511 433
Size: 30 2529
Fragments arranged by size:
2529 1090 838 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 324 1646 324
Size: 1322 3285
HindIII A'AGCT_T
Cuts at: 433 433
Size: 4607
KasI G'GCGC_C
Cuts at: 2037 2285 2037
Size: 248 4359
KpnI G_GTAC'C
Cuts at: 1627 1627
Size: 4607
MluI A'CGCG_T
Cuts at: 4040 4040
Size: 4607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 243 3278 3462 243
Size: 3035 184 1388
Fragments arranged by size:
3035 1388 184
MscI TGG'CCA
Cuts at: 1343 1516 2368 1343
Size: 173 852 3582
Fragments arranged by size:
3582 852 173
MslI CAynn'nnrTG
Cuts at: 790 2596 2723 790
Size: 1806 127 2674
Fragments arranged by size:
2674 1806 127
MspA1I CmG'CkG
Cuts at: 225 351 1225 1455 1476 1718 2032 2392 3405
Size: 126 874 230 21 242 314 360 1013
Cuts at: 3405 3650 225
Size: 245 1182
Fragments arranged by size:
1182 1013 874 360 314 245 242 230
126 21
NaeI GCC'GGC
Cuts at: 2788 2788
Size: 4607
NarI GG'CG_CC
Cuts at: 2038 2286 2038
Size: 248 4359
NgoMIV G'CCGG_C
Cuts at: 2786 2786
Size: 4607
NspI r_CATG'y
Cuts at: 1660 2160 3067 1660
Size: 500 907 3200
Fragments arranged by size:
3200 907 500
PciI A'CATG_T
Cuts at: 3063 3063
Size: 4607
PmeI GTTT'AAAC
Cuts at: 1606 1606
Size: 4607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4575
PspOMI G'GGCC_C
Cuts at: 1309 1309
Size: 4607
PstI C_TGCA'G
Cuts at: 605 1654 2339 605
Size: 1049 685 2873
Fragments arranged by size:
2873 1049 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4607
PvuII CAG'CTG
Cuts at: 225 351 1225 1455 2032 2392 225
Size: 126 874 230 577 360 2440
Fragments arranged by size:
2440 874 577 360 230 126
RsrII CG'GwC_CG
Cuts at: 2802 2802
Size: 4607
SacI G_AGCT'C
Cuts at: 269 1621 269
Size: 1352 3255
SalI G'TCGA_C
Cuts at: 322 1644 322
Size: 1322 3285
SapI GCTCTTCn'nnn_
Cuts at: 2630 2840 2630
Size: 210 4397
SbfI CC_TGCA'GG
Cuts at: 1654 1654
Size: 4607
ScaI AGT'ACT
Cuts at: 4124 4124
Size: 4607
SfcI C'TryA_G
Cuts at: 33 331 601 1121 1650 2335 3328 3519 33
Size: 298 270 520 529 685 993 191 1121
Fragments arranged by size:
1121 993 685 529 520 298 270 191
SfoI GGC'GCC
Cuts at: 2039 2287 2039
Size: 248 4359
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
SmaI CCC'GGG
Cuts at: 1050 1629 1050
Size: 579 4028
SmlI C'TyrA_G
Cuts at: 1762 2677 3169 3431 1762
Size: 915 492 262 2938
Fragments arranged by size:
2938 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4607
SphI G_CATG'C
Cuts at: 1660 2160 1660
Size: 500 4107
StyI C'CwwG_G
Cuts at: 136 359 611 1741 2095 136
Size: 223 252 1130 354 2648
Fragments arranged by size:
2648 1130 354 252 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 341 798 2190 2323 2782 2991 341
Size: 457 1392 133 459 209 1957
Fragments arranged by size:
1957 1392 459 457 209 133
TatI w'GTAC_w
Cuts at: 1547 4122 1547
Size: 2575 2032
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4124 4156 4124
Size: 32 4575
Tth111I GACn'n_nGTC
Cuts at: 2404 2404
Size: 4607
XbaI T'CTAG_A
Cuts at: 1638 1638
Size: 4607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 201 759 955 2102 201
Size: 558 196 1147 2706
Fragments arranged by size:
2706 1147 558 196
XmaI C'CCGG_G
Cuts at: 1048 1627 1048
Size: 579 4028
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbsI BbvCI BfrBI BglI BmtI Bpu10I BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI DraIII FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI