Restriction map for
pF1KB5892
(Circular) (Six-base) MAPSORT of: pF1KB5892.seq Check: 6976 from: 1 to: 4685
pF1KB5892 4685 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 182 1723 182
Size: 1541 3144
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4685
AclI AA'CG_TT
Cuts at: 4525 4525
Size: 4685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 784 836 974 2510 2942 3689 3957 426
Size: 358 52 138 1536 432 747 268 1154
Fragments arranged by size:
1536 1154 747 432 358 268 138 52
AflIII A'CryG_T
Cuts at: 499 2037 3141 4118 499
Size: 1538 1104 977 1066
Fragments arranged by size:
1538 1104 1066 977
AgeI A'CCGG_T
Cuts at: 3073 3073
Size: 4685
AhdI GACnn_n'nnGTC
Cuts at: 1278 1278
Size: 4685
AleI CACnn'nnGTG
Cuts at: 1675 1675
Size: 4685
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1175 1207 1175
Size: 32 4653
AlwNI CAG_nnn'CTG
Cuts at: 1011 1290 1990 3557 1011
Size: 279 700 1567 2139
Fragments arranged by size:
2139 1567 700 279
ApaLI G'TGCA_C
Cuts at: 1406 3455 1406
Size: 2049 2636
ApoI r'AATT_y
Cuts at: 317 395 492 1590 1689 4038 4294 317
Size: 78 97 1098 99 2349 256 708
Fragments arranged by size:
2349 1098 708 256 99 97 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 4685
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
AvaI C'yCGr_G
Cuts at: 190 262 1705 190
Size: 72 1443 3170
Fragments arranged by size:
3170 1443 72
BamHI G'GATC_C
Cuts at: 1710 1710
Size: 4685
BanI G'GyrC_C
Cuts at: 1701 2115 2363 2398 1701
Size: 414 248 35 3988
Fragments arranged by size:
3988 414 248 35
BanII G_rGCy'C
Cuts at: 693 1699 2729 693
Size: 1006 1030 2649
Fragments arranged by size:
2649 1030 1006
BbeI G_GCGC'C
Cuts at: 2119 2367 2119
Size: 248 4437
BbsI GAAGACnn'nnnn_
Cuts at: 636 1517 636
Size: 881 3804
BbvCI CC'TCA_GC
Cuts at: 1358 1358
Size: 4685
BciVI GTATCCnnnnn_n'
Cuts at: 1148 2583 3344 4685 1148
Size: 1435 761 1341 1148
Fragments arranged by size:
1435 1341 1148 761
BclI T'GATC_A
Cuts at: 2205 2205
Size: 4685
BglII A'GATC_T
Cuts at: 197 708 2200 4237 197
Size: 511 1492 2037 645
Fragments arranged by size:
2037 1492 645 511
BlpI GC'TnA_GC
Cuts at: 1797 1797
Size: 4685
Bme1580I G_kGCm'C
Cuts at: 1410 2310 2403 3459 1410
Size: 900 93 1056 2636
Fragments arranged by size:
2636 1056 900 93
BmrI ACTGGGnnnn_n'
Cuts at: 1897 1978 2313 4457 1897
Size: 81 335 2144 2125
Fragments arranged by size:
2144 2125 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 4685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 317 1432 241
Size: 76 1115 3494
Fragments arranged by size:
3494 1115 76
Bpu10I CC'TnA_GC
Cuts at: 1358 1358
Size: 4685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1861 2740 3232 3530 1861
Size: 879 492 298 3016
Fragments arranged by size:
3016 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4389 4389
Size: 4685
BsaAI yAC'GTr
Cuts at: 1135 2038 1135
Size: 903 3782
BsaBI GATnn'nnATC
Cuts at: 6 725 2199 4236 6
Size: 719 1474 2037 455
Fragments arranged by size:
2037 1474 719 455
BsaHI Gr'CG_yC
Cuts at: 239 2116 2364 3066 4562 239
Size: 1877 248 702 1496 362
Fragments arranged by size:
1877 1496 702 362 248
BsaWI w'CCGG_w
Cuts at: 882 1874 2395 3073 3347 3494 3905 882
Size: 992 521 678 274 147 411 1662
Fragments arranged by size:
1662 992 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 238 208
Size: 30 4655
BseYI C'CCAG_C
Cuts at: 620 1283 2110 2868 3445 3941 620
Size: 663 827 758 577 496 1364
Fragments arranged by size:
1364 827 758 663 577 496
BsiEI CG_ry'CG
Cuts at: 104 2227 2273 3481 104
Size: 2123 46 1208 1308
Fragments arranged by size:
2123 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1410 1699 2477 2667 3459 1410
Size: 289 778 190 792 2636
Fragments arranged by size:
2636 792 778 289 190
BsmI GAATG_Cn'
Cuts at: 1065 1614 1065
Size: 549 4136
BsmBI CGTCTCn'nnnn_
Cuts at: 1513 1513
Size: 4685
Bsp1286I G_dGCh'C
Cuts at: 693 1410 1699 2310 2403 2477 2667 2729 3459
Size: 717 289 611 93 74 190 62 730
Cuts at: 3459 693
Size: 1919
Fragments arranged by size:
1919 730 717 611 289 190 93 74
62
BspEI T'CCGG_A
Cuts at: 882 3905 882
Size: 3023 1662
BspHI T'CATG_A
Cuts at: 3861 3861
Size: 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 420 779 1167 1735 1949 2251 2632 420
Size: 359 388 568 214 302 381 2473
Fragments arranged by size:
2473 568 388 381 359 302 214
BsrBI CCG'CTC
Cuts at: 50 2977 3031 4515 4680 50
Size: 2927 54 1484 165 55
Fragments arranged by size:
2927 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2597 2597
Size: 4685
BsrFI r'CCGG_y
Cuts at: 2683 2864 3073 2683
Size: 181 209 4295
Fragments arranged by size:
4295 209 181
BssSI C'ACGA_G
Cuts at: 2956 3314 2956
Size: 358 4327
BstAPI GCAn_nnn'nTGC
Cuts at: 2062 2062
Size: 4685
BstBI TT'CG_AA
Cuts at: 3046 3046
Size: 4685
BstXI CCAn_nnnn'nTGG
Cuts at: 1677 1677
Size: 4685
BstYI r'GATC_y
Cuts at: 1 197 708 1710 2177 2200 2535 2781 3793
Size: 196 511 1002 467 23 335 246 1012
Cuts at: 3793 3879 3891 4237 1
Size: 86 12 346 449
Fragments arranged by size:
1012 1002 511 467 449 346 335 246
196 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 746 746
Size: 4685
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2635 2816 2979 2635
Size: 181 163 4341
Fragments arranged by size:
4341 181 163
BtsI GCAGTG_nn'
Cuts at: 114 168 424 1928 114
Size: 54 256 1504 2871
Fragments arranged by size:
2871 1504 256 54
DraI TTT'AAA
Cuts at: 1684 1684
Size: 4685
DrdI GACnn_nn'nnGTC
Cuts at: 2391 3249 2391
Size: 858 3827
EaeI y'GGCC_r
Cuts at: 2270 2444 2835 2862 4207 2270
Size: 174 391 27 1345 2748
Fragments arranged by size:
2748 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2270 2270
Size: 4685
EarI CTCTTCn'nnn_
Cuts at: 2708 2918 2708
Size: 210 4475
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2893 3203 3349 4495 2893
Size: 310 146 1146 3083
Fragments arranged by size:
3083 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1697 1697
Size: 4685
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 317 426 784 836 974 1432 2510 2942
Size: 76 109 358 52 138 458 1078 432
Cuts at: 2942 3689 3957 241
Size: 747 268 969
Fragments arranged by size:
1078 969 747 458 432 358 268 138
109 76 52
EcoO109I rG'GnC_Cy
Cuts at: 1824 1824
Size: 4685
EcoRI G'AATT_C
Cuts at: 1689 1689
Size: 4685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 4685
FspI TGC'GCA
Cuts at: 2466 2466
Size: 4685
HaeII r_GCGC'y
Cuts at: 168 2119 2367 3389 4368 168
Size: 1951 248 1022 979 485
Fragments arranged by size:
1951 1022 979 485 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 573 605 1264 1296 2527 2557 2559 2589 573
Size: 32 659 32 1231 30 2 30 2669
Fragments arranged by size:
2669 1231 659 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1464 1724 1464
Size: 260 4425
HindIII A'AGCT_T
Cuts at: 295 474 295
Size: 179 4506
KasI G'GCGC_C
Cuts at: 2115 2363 2115
Size: 248 4437
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4685
MluI A'CGCG_T
Cuts at: 4118 4118
Size: 4685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 666 698 782 3356 3540 666
Size: 32 84 2574 184 1811
Fragments arranged by size:
2574 1811 184 84 32
MscI TGG'CCA
Cuts at: 2446 2446
Size: 4685
MslI CAynn'nnrTG
Cuts at: 1675 2674 2801 1675
Size: 999 127 3559
Fragments arranged by size:
3559 999 127
MspA1I CmG'CkG
Cuts at: 324 357 446 624 1362 1796 2110 2470 3483
Size: 33 89 178 738 434 314 360 1013
Cuts at: 3483 3728 324
Size: 245 1281
Fragments arranged by size:
1281 1013 738 434 360 314 245 178
89 33
NaeI GCC'GGC
Cuts at: 2866 2866
Size: 4685
NarI GG'CG_CC
Cuts at: 2116 2364 2116
Size: 248 4437
NgoMIV G'CCGG_C
Cuts at: 2864 2864
Size: 4685
NspI r_CATG'y
Cuts at: 503 1738 2238 3145 503
Size: 1235 500 907 2043
Fragments arranged by size:
2043 1235 907 500
PasI CC'CwG_GG
Cuts at: 1555 1555
Size: 4685
PciI A'CATG_T
Cuts at: 499 3141 499
Size: 2642 2043
PfoI T'CCnGG_A
Cuts at: 1193 1193
Size: 4685
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4653
PsiI TTA'TAA
Cuts at: 560 560
Size: 4685
PstI C_TGCA'G
Cuts at: 431 1114 1164 1732 2417 431
Size: 683 50 568 685 2699
Fragments arranged by size:
2699 685 683 568 50
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4685
PvuII CAG'CTG
Cuts at: 357 624 1362 2110 2470 357
Size: 267 738 748 360 2572
Fragments arranged by size:
2572 748 738 360 267
RsrII CG'GwC_CG
Cuts at: 2880 2880
Size: 4685
SacI G_AGCT'C
Cuts at: 1699 1699
Size: 4685
SalI G'TCGA_C
Cuts at: 1722 1722
Size: 4685
SapI GCTCTTCn'nnn_
Cuts at: 2708 2918 2708
Size: 210 4475
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4685
ScaI AGT'ACT
Cuts at: 4202 4202
Size: 4685
SfcI C'TryA_G
Cuts at: 33 427 526 1110 1160 1728 2413 3406 3597
Size: 394 99 584 50 568 685 993 191
Cuts at: 3597 33
Size: 1121
Fragments arranged by size:
1121 993 685 584 568 394 191 99
50
SfoI GGC'GCC
Cuts at: 2117 2365 2117
Size: 248 4437
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
SmaI CCC'GGG
Cuts at: 1707 1707
Size: 4685
SmlI C'TyrA_G
Cuts at: 1840 2755 3247 3509 1840
Size: 915 492 262 3016
Fragments arranged by size:
3016 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4685
SphI G_CATG'C
Cuts at: 1738 2238 1738
Size: 500 4185
SspI AAT'ATT
Cuts at: 546 546
Size: 4685
StyI C'CwwG_G
Cuts at: 1819 2173 1819
Size: 354 4331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1568 1676 2268 2401 2860 3069 1568
Size: 108 592 133 459 209 3184
Fragments arranged by size:
3184 592 459 209 133 108
TatI w'GTAC_w
Cuts at: 4200 4200
Size: 4685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1440 1472 4202 4234 1440
Size: 32 2730 32 1891
Fragments arranged by size:
2730 1891 32 32
Tth111I GACn'n_nGTC
Cuts at: 2482 2482
Size: 4685
XbaI T'CTAG_A
Cuts at: 1716 1716
Size: 4685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2180 2180
Size: 4685
XmaI C'CCGG_G
Cuts at: 1705 1705
Size: 4685
XmnI GAAnn'nnTTC
Cuts at: 476 809 878 476
Size: 333 69 4283
Fragments arranged by size:
4283 333 69
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsrGI BssHII BstEII BstZ17I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI