Restriction map for
pF1KB5894
(Circular) (Six-base) MAPSORT of: pF1KB5894.seq Check: 8440 from: 1 to: 4685
pF1KB5894 4685 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1723 1723
Size: 4685
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4685
AclI AA'CG_TT
Cuts at: 4525 4525
Size: 4685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 2510 2942 3689 3957 784
Size: 1726 432 747 268 1512
Fragments arranged by size:
1726 1512 747 432 268
AflIII A'CryG_T
Cuts at: 237 2037 3141 4118 237
Size: 1800 1104 977 804
Fragments arranged by size:
1800 1104 977 804
AgeI A'CCGG_T
Cuts at: 3073 3073
Size: 4685
AleI CACnn'nnGTG
Cuts at: 157 157
Size: 4685
AlwNI CAG_nnn'CTG
Cuts at: 807 1675 1990 3557 807
Size: 868 315 1567 1935
Fragments arranged by size:
1935 1567 868 315
ApaLI G'TGCA_C
Cuts at: 3455 3455
Size: 4685
ApoI r'AATT_y
Cuts at: 733 1600 1654 1689 4038 4294 733
Size: 867 54 35 2349 256 1124
Fragments arranged by size:
2349 1124 867 256 54 35
AseI AT'TA_AT
Cuts at: 20 926 20
Size: 906 3779
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
AvaI C'yCGr_G
Cuts at: 1705 1705
Size: 4685
AvrII C'CTAG_G
Cuts at: 1659 1659
Size: 4685
BamHI G'GATC_C
Cuts at: 1710 1710
Size: 4685
BanI G'GyrC_C
Cuts at: 1701 2115 2363 2398 1701
Size: 414 248 35 3988
Fragments arranged by size:
3988 414 248 35
BanII G_rGCy'C
Cuts at: 525 1699 2729 525
Size: 1174 1030 2481
Fragments arranged by size:
2481 1174 1030
BbeI G_GCGC'C
Cuts at: 2119 2367 2119
Size: 248 4437
BbvCI CC'TCA_GC
Cuts at: 353 353
Size: 4685
BciVI GTATCCnnnnn_n'
Cuts at: 420 2583 3344 4685 420
Size: 2163 761 1341 420
Fragments arranged by size:
2163 1341 761 420
BclI T'GATC_A
Cuts at: 197 2205 197
Size: 2008 2677
BglII A'GATC_T
Cuts at: 2200 4237 2200
Size: 2037 2648
BlpI GC'TnA_GC
Cuts at: 1046 1620 1797 1046
Size: 574 177 3934
Fragments arranged by size:
3934 574 177
Bme1580I G_kGCm'C
Cuts at: 2310 2403 3459 2310
Size: 93 1056 3536
Fragments arranged by size:
3536 1056 93
BmgBI CAC'GTC
Cuts at: 1402 1402
Size: 4685
BmrI ACTGGGnnnn_n'
Cuts at: 137 1897 1978 2313 4457 137
Size: 1760 81 335 2144 365
Fragments arranged by size:
2144 1760 365 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 4685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 1538
Size: 4685
Bpu10I CC'TnA_GC
Cuts at: 353 353
Size: 4685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1861 2740 3232 3530 1861
Size: 879 492 298 3016
Fragments arranged by size:
3016 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4389 4389
Size: 4685
BsaAI yAC'GTr
Cuts at: 2038 2038
Size: 4685
BsaBI GATnn'nnATC
Cuts at: 6 1315 2199 4236 6
Size: 1309 884 2037 455
Fragments arranged by size:
2037 1309 884 455
BsaHI Gr'CG_yC
Cuts at: 2116 2364 3066 4562 2116
Size: 248 702 1496 2239
Fragments arranged by size:
2239 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1057 1874 2395 3073 3347 3494 3905 1057
Size: 817 521 678 274 147 411 1837
Fragments arranged by size:
1837 817 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 162 2110 2868 3445 3941 162
Size: 1948 758 577 496 906
Fragments arranged by size:
1948 906 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2227 2273 3481 104
Size: 2123 46 1208 1308
Fragments arranged by size:
2123 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 525 669 1526 1699 2477 2667 3459 525
Size: 144 857 173 778 190 792 1751
Fragments arranged by size:
1751 857 792 778 190 173 144
BsmI GAATG_Cn'
Cuts at: 704 1426 704
Size: 722 3963
Bsp1286I G_dGCh'C
Cuts at: 525 669 1526 1699 2310 2403 2477 2667 2729
Size: 144 857 173 611 93 74 190 62
Cuts at: 2729 3459 525
Size: 730 1751
Fragments arranged by size:
1751 857 730 611 190 173 144 93
74 62
BspEI T'CCGG_A
Cuts at: 1057 3905 1057
Size: 2848 1837
BspHI T'CATG_A
Cuts at: 3861 3861
Size: 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1450 1735 1949 2251 2632 1450
Size: 285 214 302 381 3503
Fragments arranged by size:
3503 381 302 285 214
BsrBI CCG'CTC
Cuts at: 50 655 2977 3031 4515 4680 50
Size: 605 2322 54 1484 165 55
Fragments arranged by size:
2322 1484 605 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1475 2597 1475
Size: 1122 3563
BsrFI r'CCGG_y
Cuts at: 2683 2864 3073 2683
Size: 181 209 4295
Fragments arranged by size:
4295 209 181
BssSI C'ACGA_G
Cuts at: 2956 3314 2956
Size: 358 4327
BstAPI GCAn_nnn'nTGC
Cuts at: 768 1423 2062 768
Size: 655 639 3391
Fragments arranged by size:
3391 655 639
BstBI TT'CG_AA
Cuts at: 3046 3046
Size: 4685
BstXI CCAn_nnnn'nTGG
Cuts at: 347 347
Size: 4685
BstYI r'GATC_y
Cuts at: 1 1710 2177 2200 2535 2781 3793 3879 3891
Size: 1709 467 23 335 246 1012 86 12
Cuts at: 3891 4237 1
Size: 346 449
Fragments arranged by size:
1709 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2635 2816 2979 2635
Size: 181 163 4341
Fragments arranged by size:
4341 181 163
BtsI GCAGTG_nn'
Cuts at: 152 1163 1928 152
Size: 1011 765 2909
Fragments arranged by size:
2909 1011 765
DraI TTT'AAA
Cuts at: 1554 1684 1554
Size: 130 4555
DrdI GACnn_nn'nnGTC
Cuts at: 2391 3249 2391
Size: 858 3827
EaeI y'GGCC_r
Cuts at: 689 2270 2444 2835 2862 4207 689
Size: 1581 174 391 27 1345 1167
Fragments arranged by size:
1581 1345 1167 391 174 27
EagI C'GGCC_G
Cuts at: 2270 2270
Size: 4685
EarI CTCTTCn'nnn_
Cuts at: 353 994 1606 2708 2918 353
Size: 641 612 1102 210 2120
Fragments arranged by size:
2120 1102 641 612 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2893 3203 3349 4495 2893
Size: 310 146 1146 3083
Fragments arranged by size:
3083 1146 310 146
EcoICRI GAG'CTC
Cuts at: 523 1697 523
Size: 1174 3511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1538 2510 2942 3689 3957 784
Size: 754 972 432 747 268 1512
Fragments arranged by size:
1512 972 754 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 131 1824 131
Size: 1693 2992
EcoRI G'AATT_C
Cuts at: 1600 1689 1600
Size: 89 4596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 4685
FspI TGC'GCA
Cuts at: 2466 2466
Size: 4685
HaeII r_GCGC'y
Cuts at: 1148 2119 2367 3389 4368 1148
Size: 971 248 1022 979 1465
Fragments arranged by size:
1465 1022 979 971 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 711 743 1193 1225 2527 2557 2559 2589 711
Size: 32 450 32 1302 30 2 30 2807
Fragments arranged by size:
2807 1302 450 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1724 1724
Size: 4685
HindIII A'AGCT_T
Cuts at: 882 882
Size: 4685
KasI G'GCGC_C
Cuts at: 2115 2363 2115
Size: 248 4437
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4685
MfeI C'AATT_G
Cuts at: 1434 1434
Size: 4685
MluI A'CGCG_T
Cuts at: 4118 4118
Size: 4685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 498 673 3356 3540 498
Size: 175 2683 184 1643
Fragments arranged by size:
2683 1643 184 175
MscI TGG'CCA
Cuts at: 691 2446 691
Size: 1755 2930
MslI CAynn'nnrTG
Cuts at: 157 193 1252 1258 2674 2801 157
Size: 36 1059 6 1416 127 2041
Fragments arranged by size:
2041 1416 1059 127 36 6
MspA1I CmG'CkG
Cuts at: 373 687 1796 2110 2470 3483 3728 373
Size: 314 1109 314 360 1013 245 1330
Fragments arranged by size:
1330 1109 1013 360 314 314 245
NaeI GCC'GGC
Cuts at: 2866 2866
Size: 4685
NarI GG'CG_CC
Cuts at: 2116 2364 2116
Size: 248 4437
NgoMIV G'CCGG_C
Cuts at: 2864 2864
Size: 4685
NspI r_CATG'y
Cuts at: 241 1042 1738 2238 3145 241
Size: 801 696 500 907 1781
Fragments arranged by size:
1781 907 801 696 500
PciI A'CATG_T
Cuts at: 237 3141 237
Size: 2904 1781
PflMI CCAn_nnn'nTGG
Cuts at: 395 395
Size: 4685
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4653
PpuMI rG'GwC_Cy
Cuts at: 131 131
Size: 4685
PsiI TTA'TAA
Cuts at: 568 568
Size: 4685
PstI C_TGCA'G
Cuts at: 1599 1732 2417 1599
Size: 133 685 3867
Fragments arranged by size:
3867 685 133
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4685
PvuII CAG'CTG
Cuts at: 373 687 2110 2470 373
Size: 314 1423 360 2588
Fragments arranged by size:
2588 1423 360 314
RsrII CG'GwC_CG
Cuts at: 2880 2880
Size: 4685
SacI G_AGCT'C
Cuts at: 525 1699 525
Size: 1174 3511
SalI G'TCGA_C
Cuts at: 1722 1722
Size: 4685
SanDI GG'GwC_CC
Cuts at: 131 131
Size: 4685
SapI GCTCTTCn'nnn_
Cuts at: 994 2708 2918 994
Size: 1714 210 2761
Fragments arranged by size:
2761 1714 210
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4685
ScaI AGT'ACT
Cuts at: 4202 4202
Size: 4685
SfcI C'TryA_G
Cuts at: 33 231 1595 1643 1667 1728 2413 3406 3597
Size: 198 1364 48 24 61 685 993 191
Cuts at: 3597 33
Size: 1121
Fragments arranged by size:
1364 1121 993 685 198 191 61 48
24
SfoI GGC'GCC
Cuts at: 2117 2365 2117
Size: 248 4437
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
SmaI CCC'GGG
Cuts at: 1707 1707
Size: 4685
SmlI C'TyrA_G
Cuts at: 1840 2755 3247 3509 1840
Size: 915 492 262 3016
Fragments arranged by size:
3016 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4685
SphI G_CATG'C
Cuts at: 1738 2238 1738
Size: 500 4185
StuI AGG'CCT
Cuts at: 617 617
Size: 4685
StyI C'CwwG_G
Cuts at: 346 1659 1819 2173 346
Size: 1313 160 354 2858
Fragments arranged by size:
2858 1313 354 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 235 278 443 2268 2401 2860 3069 235
Size: 43 165 1825 133 459 209 1851
Fragments arranged by size:
1851 1825 459 209 165 133 43
TatI w'GTAC_w
Cuts at: 295 1472 4200 295
Size: 1177 2728 780
Fragments arranged by size:
2728 1177 780
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1484 1516 4202 4234 1484
Size: 32 2686 32 1935
Fragments arranged by size:
2686 1935 32 32
Tth111I GACn'n_nGTC
Cuts at: 1400 2482 1400
Size: 1082 3603
XbaI T'CTAG_A
Cuts at: 1716 1716
Size: 4685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1612 2180 1612
Size: 568 4117
XmaI C'CCGG_G
Cuts at: 1705 1705
Size: 4685
XmnI GAAnn'nnTTC
Cuts at: 1282 1282
Size: 4685
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BaeI BbsI BcgI BfrBI BglI BmtI BsaXI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI