Restriction map for
pF1KB5901
(Circular) (Six-base) MAPSORT of: pF1KB5901.seq Check: 1338 from: 1 to: 4613
pF1KB5901 4613 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1120 1651 1120
Size: 531 4082
Acc65I G'GTAC_C
Cuts at: 1629 1629
Size: 4613
AclI AA'CG_TT
Cuts at: 4453 4453
Size: 4613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2438 2870 3617 3885 1171
Size: 1267 432 747 268 1899
Fragments arranged by size:
1899 1267 747 432 268
AflII C'TTAA_G
Cuts at: 976 976
Size: 4613
AflIII A'CryG_T
Cuts at: 1965 3069 4046 1965
Size: 1104 977 2532
Fragments arranged by size:
2532 1104 977
AgeI A'CCGG_T
Cuts at: 3001 3001
Size: 4613
AhdI GACnn_n'nnGTC
Cuts at: 847 847
Size: 4613
AleI CACnn'nnGTG
Cuts at: 753 1165 753
Size: 412 4201
AlwNI CAG_nnn'CTG
Cuts at: 871 1918 3485 871
Size: 1047 1567 1999
Fragments arranged by size:
1999 1567 1047
ApaI G_GGCC'C
Cuts at: 1144 1144
Size: 4613
ApaLI G'TGCA_C
Cuts at: 3383 3383
Size: 4613
ApoI r'AATT_y
Cuts at: 169 808 1236 1248 1566 1617 3966 4222 169
Size: 639 428 12 318 51 2349 256 560
Fragments arranged by size:
2349 639 560 428 318 256 51 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4613
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
AvaI C'yCGr_G
Cuts at: 1633 1633
Size: 4613
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1586 1619 1586
Size: 33 4580
BamHI G'GATC_C
Cuts at: 1638 1638
Size: 4613
BanI G'GyrC_C
Cuts at: 1509 1629 2043 2291 2326 1509
Size: 120 414 248 35 3796
Fragments arranged by size:
3796 414 248 120 35
BanII G_rGCy'C
Cuts at: 127 1144 1627 2657 127
Size: 1017 483 1030 2083
Fragments arranged by size:
2083 1030 1017 483
BbeI G_GCGC'C
Cuts at: 2047 2295 2047
Size: 248 4365
BbvCI CC'TCA_GC
Cuts at: 577 577
Size: 4613
BciVI GTATCCnnnnn_n'
Cuts at: 975 1614 2511 3272 4613 975
Size: 639 897 761 1341 975
Fragments arranged by size:
1341 975 897 761 639
BclI T'GATC_A
Cuts at: 2133 2133
Size: 4613
BfrBI ATG'CAT
Cuts at: 647 647
Size: 4613
BglII A'GATC_T
Cuts at: 2128 4165 2128
Size: 2037 2576
BlpI GC'TnA_GC
Cuts at: 1725 1725
Size: 4613
Bme1580I G_kGCm'C
Cuts at: 1144 1514 2238 2331 3387 1144
Size: 370 724 93 1056 2370
Fragments arranged by size:
2370 1056 724 370 93
BmrI ACTGGGnnnn_n'
Cuts at: 1150 1825 1906 2241 4385 1150
Size: 675 81 335 2144 1378
Fragments arranged by size:
2144 1378 675 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 4613
Bpu10I CC'TnA_GC
Cuts at: 577 577
Size: 4613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 1789 2668 3160 3458 995
Size: 794 879 492 298 2150
Fragments arranged by size:
2150 879 794 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4317 4317
Size: 4613
BsaAI yAC'GTr
Cuts at: 1966 1966
Size: 4613
BsaBI GATnn'nnATC
Cuts at: 6 2127 4164 6
Size: 2121 2037 455
Fragments arranged by size:
2121 2037 455
BsaHI Gr'CG_yC
Cuts at: 2044 2292 2994 4490 2044
Size: 248 702 1496 2167
Fragments arranged by size:
2167 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1802 2323 3001 3275 3422 3833 1802
Size: 521 678 274 147 411 2582
Fragments arranged by size:
2582 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 151
Size: 4613
BseYI C'CCAG_C
Cuts at: 2038 2796 3373 3869 2038
Size: 758 577 496 2782
Fragments arranged by size:
2782 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1493 1493
Size: 4613
BsiEI CG_ry'CG
Cuts at: 104 2155 2201 3409 104
Size: 2051 46 1208 1308
Fragments arranged by size:
2051 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 865 1627 2405 2595 3387 865
Size: 762 778 190 792 2091
Fragments arranged by size:
2091 792 778 762 190
BsmI GAATG_Cn'
Cuts at: 1336 1336
Size: 4613
Bsp1286I G_dGCh'C
Cuts at: 127 865 1144 1514 1627 2238 2331 2405 2595
Size: 738 279 370 113 611 93 74 190
Cuts at: 2595 2657 3387 127
Size: 62 730 1353
Fragments arranged by size:
1353 738 730 611 370 279 190 113
93 74 62
BspEI T'CCGG_A
Cuts at: 3833 3833
Size: 4613
BspHI T'CATG_A
Cuts at: 3789 3789
Size: 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1663 1877 2179 2560 1663
Size: 214 302 381 3716
Fragments arranged by size:
3716 381 302 214
BsrBI CCG'CTC
Cuts at: 50 180 2905 2959 4443 4608 50
Size: 130 2725 54 1484 165 55
Fragments arranged by size:
2725 1484 165 130 55 54
BsrDI GCAATG_nn'
Cuts at: 647 1220 1464 2525 647
Size: 573 244 1061 2735
Fragments arranged by size:
2735 1061 573 244
BsrFI r'CCGG_y
Cuts at: 2611 2792 3001 2611
Size: 181 209 4223
Fragments arranged by size:
4223 209 181
BsrGI T'GTAC_A
Cuts at: 1289 1289
Size: 4613
BssSI C'ACGA_G
Cuts at: 2884 3242 2884
Size: 358 4255
BstAPI GCAn_nnn'nTGC
Cuts at: 271 1990 271
Size: 1719 2894
BstBI TT'CG_AA
Cuts at: 2974 2974
Size: 4613
BstYI r'GATC_y
Cuts at: 1 1638 2105 2128 2463 2709 3721 3807 3819
Size: 1637 467 23 335 246 1012 86 12
Cuts at: 3819 4165 1
Size: 346 449
Fragments arranged by size:
1637 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1121 1121
Size: 4613
Bsu36I CC'TnA_GG
Cuts at: 654 654
Size: 4613
BtgI C'CryG_G
Cuts at: 107 131 1456 107
Size: 24 1325 3264
Fragments arranged by size:
3264 1325 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 143 389 2563 2744 2907 143
Size: 246 2174 181 163 1849
Fragments arranged by size:
2174 1849 246 181 163
BtsI GCAGTG_nn'
Cuts at: 345 760 1476 1856 345
Size: 415 716 380 3102
Fragments arranged by size:
3102 716 415 380
DraI TTT'AAA
Cuts at: 227 1612 227
Size: 1385 3228
DrdI GACnn_nn'nnGTC
Cuts at: 2319 3177 2319
Size: 858 3755
EaeI y'GGCC_r
Cuts at: 1459 2198 2372 2763 2790 4135 1459
Size: 739 174 391 27 1345 1937
Fragments arranged by size:
1937 1345 739 391 174 27
EagI C'GGCC_G
Cuts at: 2198 2198
Size: 4613
EarI CTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2821 3131 3277 4423 2821
Size: 310 146 1146 3011
Fragments arranged by size:
3011 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1625 1625
Size: 4613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2438 2870 3617 3885 1171
Size: 1267 432 747 268 1899
Fragments arranged by size:
1899 1267 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1141 1752 1141
Size: 611 4002
EcoRI G'AATT_C
Cuts at: 808 1617 808
Size: 809 3804
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4613
FspI TGC'GCA
Cuts at: 149 2394 149
Size: 2245 2368
HaeII r_GCGC'y
Cuts at: 1085 2047 2295 3317 4296 1085
Size: 962 248 1022 979 1402
Fragments arranged by size:
1402 1022 979 962 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 778 810 833 865 931 963 2455 2485 2487
Size: 32 23 32 66 32 1492 30 2
Cuts at: 2487 2517 778
Size: 30 2874
Fragments arranged by size:
2874 1492 66 32 32 32 30 30
23 2
HincII GTy'rAC
Cuts at: 1652 1652
Size: 4613
KasI G'GCGC_C
Cuts at: 2043 2291 2043
Size: 248 4365
KpnI G_GTAC'C
Cuts at: 1633 1633
Size: 4613
MluI A'CGCG_T
Cuts at: 4046 4046
Size: 4613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1330 3284 3468 1330
Size: 1954 184 2475
Fragments arranged by size:
2475 1954 184
MscI TGG'CCA
Cuts at: 2374 2374
Size: 4613
MslI CAynn'nnrTG
Cuts at: 568 753 962 1165 1469 2602 2729 568
Size: 185 209 203 304 1133 127 2452
Fragments arranged by size:
2452 1133 304 209 203 185 127
MspA1I CmG'CkG
Cuts at: 133 1458 1724 2038 2398 3411 3656 133
Size: 1325 266 314 360 1013 245 1090
Fragments arranged by size:
1325 1090 1013 360 314 266 245
NaeI GCC'GGC
Cuts at: 2794 2794
Size: 4613
NarI GG'CG_CC
Cuts at: 2044 2292 2044
Size: 248 4365
NcoI C'CATG_G
Cuts at: 107 107
Size: 4613
NdeI CA'TA_TG
Cuts at: 285 285
Size: 4613
NgoMIV G'CCGG_C
Cuts at: 2792 2792
Size: 4613
NsiI A_TGCA'T
Cuts at: 649 649
Size: 4613
NspI r_CATG'y
Cuts at: 154 721 1666 2166 3073 154
Size: 567 945 500 907 1694
Fragments arranged by size:
1694 945 907 567 500
PciI A'CATG_T
Cuts at: 3069 3069
Size: 4613
PflMI CCAn_nnn'nTGG
Cuts at: 163 163
Size: 4613
PfoI T'CCnGG_A
Cuts at: 1087 1087
Size: 4613
PmeI GTTT'AAAC
Cuts at: 1612 1612
Size: 4613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4581
PspOMI G'GGCC_C
Cuts at: 1140 1140
Size: 4613
PstI C_TGCA'G
Cuts at: 1660 2345 1660
Size: 685 3928
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4613
PvuII CAG'CTG
Cuts at: 2038 2398 2038
Size: 360 4253
RsrII CG'GwC_CG
Cuts at: 2808 2808
Size: 4613
SacI G_AGCT'C
Cuts at: 1627 1627
Size: 4613
SacII CC_GC'GG
Cuts at: 134 1459 134
Size: 1325 3288
SalI G'TCGA_C
Cuts at: 1650 1650
Size: 4613
SapI GCTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
SbfI CC_TGCA'GG
Cuts at: 1660 1660
Size: 4613
ScaI AGT'ACT
Cuts at: 251 4130 251
Size: 3879 734
SfcI C'TryA_G
Cuts at: 33 1589 1656 2341 3334 3525 33
Size: 1556 67 685 993 191 1121
Fragments arranged by size:
1556 1121 993 685 191 67
SfoI GGC'GCC
Cuts at: 2045 2293 2045
Size: 248 4365
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
SmaI CCC'GGG
Cuts at: 1635 1635
Size: 4613
SmlI C'TyrA_G
Cuts at: 976 1010 1768 2683 3175 3437 976
Size: 34 758 915 492 262 2152
Fragments arranged by size:
2152 915 758 492 262 34
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4613
SphI G_CATG'C
Cuts at: 154 1666 2166 154
Size: 1512 500 2601
Fragments arranged by size:
2601 1512 500
StyI C'CwwG_G
Cuts at: 107 1747 2101 107
Size: 1640 354 2619
Fragments arranged by size:
2619 1640 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2196 2329 2788 2997 2196
Size: 133 459 209 3812
Fragments arranged by size:
3812 459 209 133
TatI w'GTAC_w
Cuts at: 249 1289 4128 249
Size: 1040 2839 734
Fragments arranged by size:
2839 1040 734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 159 191 650 682 1029 1061 4130 4162 159
Size: 32 459 32 347 32 3069 32 610
Fragments arranged by size:
3069 610 459 347 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2410 2410
Size: 4613
XbaI T'CTAG_A
Cuts at: 1644 1644
Size: 4613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2108
Size: 4613
XmaI C'CCGG_G
Cuts at: 1633 1633
Size: 4613
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI AscI AvrII BbsI
BcgI BglI BmgBI BmtI BpmI BsaXI BsiWI BsmBI
BssHII BstEII BstXI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI