Restriction map for
pF1KB5907
(Circular) (Six-base) MAPSORT of: pF1KB5907.seq Check: 5138 from: 1 to: 4625
pF1KB5907 4625 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 253 1663 253
Size: 1410 3215
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 772 788 2450 2882 3629 3897 243
Size: 529 16 1662 432 747 268 971
Fragments arranged by size:
1662 971 747 529 432 268 16
AflIII A'CryG_T
Cuts at: 1977 3081 4058 1977
Size: 1104 977 2544
Fragments arranged by size:
2544 1104 977
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 147 147
Size: 4625
AlwNI CAG_nnn'CTG
Cuts at: 168 750 1930 3497 168
Size: 582 1180 1567 1296
Fragments arranged by size:
1567 1296 1180 582
ApaLI G'TGCA_C
Cuts at: 122 3395 122
Size: 3273 1352
ApoI r'AATT_y
Cuts at: 723 785 1629 3978 4234 723
Size: 62 844 2349 256 1114
Fragments arranged by size:
2349 1114 844 256 62
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 671 683 1098 1645 671
Size: 12 415 547 3651
Fragments arranged by size:
3651 547 415 12
AvrII C'CTAG_G
Cuts at: 1063 1063
Size: 4625
BamHI G'GATC_C
Cuts at: 1650 1650
Size: 4625
BanI G'GyrC_C
Cuts at: 472 1303 1408 1641 2055 2303 2338 472
Size: 831 105 233 414 248 35 2759
Fragments arranged by size:
2759 831 414 248 233 105 35
BanII G_rGCy'C
Cuts at: 802 1639 2669 802
Size: 837 1030 2758
Fragments arranged by size:
2758 1030 837
BbeI G_GCGC'C
Cuts at: 2059 2307 2059
Size: 248 4377
BbvCI CC'TCA_GC
Cuts at: 1437 1437
Size: 4625
BciVI GTATCCnnnnn_n'
Cuts at: 2523 3284 4625 2523
Size: 761 1341 2523
Fragments arranged by size:
2523 1341 761
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BglI GCCn_nnn'nGGC
Cuts at: 806 806
Size: 4625
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 212 1737 212
Size: 1525 3100
Bme1580I G_kGCm'C
Cuts at: 126 475 2250 2343 3399 126
Size: 349 1775 93 1056 1352
Fragments arranged by size:
1775 1352 1056 349 93
BmrI ACTGGGnnnn_n'
Cuts at: 1837 1918 2253 4397 1837
Size: 81 335 2144 2065
Fragments arranged by size:
2144 2065 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 886 2992 886
Size: 2106 2519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 412 583 687 1211 1573 382
Size: 30 171 104 524 362 3434
Fragments arranged by size:
3434 524 362 171 104 30
Bpu10I CC'TnA_GC
Cuts at: 217 1437 217
Size: 1220 3405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1801 2680 3172 3470 1801
Size: 879 492 298 2956
Fragments arranged by size:
2956 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 436 917 949 4329 436
Size: 481 32 3380 732
Fragments arranged by size:
3380 732 481 32
BsaAI yAC'GTr
Cuts at: 719 962 1978 719
Size: 243 1016 3366
Fragments arranged by size:
3366 1016 243
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 2056 2304 3006 4502 2056
Size: 248 702 1496 2179
Fragments arranged by size:
2179 1496 702 248
BsaWI w'CCGG_w
Cuts at: 835 1814 2335 3013 3287 3434 3845 835
Size: 979 521 678 274 147 411 1615
Fragments arranged by size:
1615 979 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 836 866 946 976 836
Size: 30 80 30 4485
Fragments arranged by size:
4485 80 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 532 391
Size: 141 4484
BseYI C'CCAG_C
Cuts at: 468 2050 2808 3385 3881 468
Size: 1582 758 577 496 1212
Fragments arranged by size:
1582 1212 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2167 2213 3421 104
Size: 2063 46 1208 1308
Fragments arranged by size:
2063 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 126 1639 2417 2607 3399 126
Size: 1513 778 190 792 1352
Fragments arranged by size:
1513 1352 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 126 475 802 1639 2250 2343 2417 2607 2669
Size: 349 327 837 611 93 74 190 62
Cuts at: 2669 3399 126
Size: 730 1352
Fragments arranged by size:
1352 837 730 611 349 327 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1675 1889 2191 2572 1675
Size: 214 302 381 3728
Fragments arranged by size:
3728 381 302 214
BsrBI CCG'CTC
Cuts at: 50 489 2917 2971 4455 4620 50
Size: 439 2428 54 1484 165 55
Fragments arranged by size:
2428 1484 439 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2537 2537
Size: 4625
BsrFI r'CCGG_y
Cuts at: 2623 2804 3013 2623
Size: 181 209 4235
Fragments arranged by size:
4235 209 181
BsrGI T'GTAC_A
Cuts at: 1415 1415
Size: 4625
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 2002 2002
Size: 4625
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 519 615 519
Size: 96 4529
BstYI r'GATC_y
Cuts at: 1 1650 2117 2140 2475 2721 3733 3819 3831
Size: 1649 467 23 335 246 1012 86 12
Cuts at: 3831 4177 1
Size: 346 449
Fragments arranged by size:
1649 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 254 254
Size: 4625
Bsu36I CC'TnA_GG
Cuts at: 478 478
Size: 4625
BtgI C'CryG_G
Cuts at: 1203 1203
Size: 4625
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 677 1868 677
Size: 1191 3434
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 240 266 605 2210 2384 2775 2802 4147 240
Size: 26 339 1605 174 391 27 1345 718
Fragments arranged by size:
1605 1345 718 391 339 174 27 26
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 563 2648 2858 563
Size: 2085 210 2330
Fragments arranged by size:
2330 2085 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 982 2833 3143 3289 4435 982
Size: 1851 310 146 1146 1172
Fragments arranged by size:
1851 1172 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1637 1637
Size: 4625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 382 412 583 687 772 788 1211 1573
Size: 139 30 171 104 85 16 423 362
Cuts at: 1573 2450 2882 3629 3897 243
Size: 877 432 747 268 971
Fragments arranged by size:
971 877 747 432 423 362 268 171
139 104 85 30 16
EcoO109I rG'GnC_Cy
Cuts at: 434 1764 434
Size: 1330 3295
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1581 2644 1581
Size: 1063 3562
FspI TGC'GCA
Cuts at: 2406 2406
Size: 4625
HaeII r_GCGC'y
Cuts at: 1124 2059 2307 3329 4308 1124
Size: 935 248 1022 979 1441
Fragments arranged by size:
1441 1022 979 935 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 787 819 2467 2497 2499 2529 787
Size: 32 1648 30 2 30 2883
Fragments arranged by size:
2883 1648 32 30 30 2
HincII GTy'rAC
Cuts at: 1455 1664 1455
Size: 209 4416
KasI G'GCGC_C
Cuts at: 2055 2303 2055
Size: 248 4377
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MfeI C'AATT_G
Cuts at: 409 409
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 500 596 3296 3480 500
Size: 96 2700 184 1645
Fragments arranged by size:
2700 1645 184 96
MscI TGG'CCA
Cuts at: 268 607 2386 268
Size: 339 1779 2507
Fragments arranged by size:
2507 1779 339
MslI CAynn'nnrTG
Cuts at: 2614 2741 2614
Size: 127 4498
MspA1I CmG'CkG
Cuts at: 157 165 287 498 1736 2050 2410 3423 3668
Size: 8 122 211 1238 314 360 1013 245
Cuts at: 3668 157
Size: 1114
Fragments arranged by size:
1238 1114 1013 360 314 245 211 122
8
NaeI GCC'GGC
Cuts at: 2806 2806
Size: 4625
NarI GG'CG_CC
Cuts at: 2056 2304 2056
Size: 248 4377
NgoMIV G'CCGG_C
Cuts at: 2804 2804
Size: 4625
NspI r_CATG'y
Cuts at: 1678 2178 3085 1678
Size: 500 907 3218
Fragments arranged by size:
3218 907 500
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PfoI T'CCnGG_A
Cuts at: 743 743
Size: 4625
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PmlI CAC'GTG
Cuts at: 962 962
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PstI C_TGCA'G
Cuts at: 136 461 1672 2357 136
Size: 325 1211 685 2404
Fragments arranged by size:
2404 1211 685 325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 157 165 287 498 2050 2410 157
Size: 8 122 211 1552 360 2372
Fragments arranged by size:
2372 1552 360 211 122 8
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 1639 1639
Size: 4625
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 2648 2858 2648
Size: 210 4415
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 4142 4142
Size: 4625
SfcI C'TryA_G
Cuts at: 33 132 457 531 1668 2353 3346 3537 33
Size: 99 325 74 1137 685 993 191 1121
Fragments arranged by size:
1137 1121 993 685 325 191 99 74
SfoI GGC'GCC
Cuts at: 2057 2305 2057
Size: 248 4377
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 1780 2695 3187 3449 1780
Size: 915 492 262 2956
Fragments arranged by size:
2956 915 492 262
SpeI A'CTAG_T
Cuts at: 67 849 67
Size: 782 3843
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StuI AGG'CCT
Cuts at: 1268 1268
Size: 4625
StyI C'CwwG_G
Cuts at: 416 1063 1759 2113 416
Size: 647 696 354 2928
Fragments arranged by size:
2928 696 647 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 964 2208 2341 2800 3009 361
Size: 603 1244 133 459 209 1977
Fragments arranged by size:
1977 1244 603 459 209 133
TatI w'GTAC_w
Cuts at: 1094 1415 1523 4140 1094
Size: 321 108 2617 1579
Fragments arranged by size:
2617 1579 321 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 946 978 4142 4174 946
Size: 32 3164 32 1397
Fragments arranged by size:
3164 1397 32 32
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2120
Size: 4625
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbsI BcgI BfrBI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BssHII BstEII ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI