Restriction map for
pF1KB5909
(Circular) (Six-base) MAPSORT of: pF1KB5909.seq Check: 1664 from: 1 to: 4625
pF1KB5909 4625 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 723 723
Size: 4625
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 220 1447 1641 220
Size: 1227 194 3204
Fragments arranged by size:
3204 1227 194
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2450 2882 3629 3897 2450
Size: 432 747 268 3178
Fragments arranged by size:
3178 747 432 268
AflII C'TTAA_G
Cuts at: 1378 1378
Size: 4625
AflIII A'CryG_T
Cuts at: 1977 3081 4058 1977
Size: 1104 977 2544
Fragments arranged by size:
2544 1104 977
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AleI CACnn'nnGTG
Cuts at: 152 913 1135 1546 152
Size: 761 222 411 3231
Fragments arranged by size:
3231 761 411 222
AlwNI CAG_nnn'CTG
Cuts at: 393 939 1339 1930 3497 393
Size: 546 400 591 1567 1521
Fragments arranged by size:
1567 1521 591 546 400
ApaI G_GGCC'C
Cuts at: 1020 1288 1020
Size: 268 4357
ApaLI G'TGCA_C
Cuts at: 1172 3395 1172
Size: 2223 2402
ApoI r'AATT_y
Cuts at: 1629 3978 4234 1629
Size: 2349 256 2020
Fragments arranged by size:
2349 2020 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 488 1281 1645 488
Size: 793 364 3468
Fragments arranged by size:
3468 793 364
BamHI G'GATC_C
Cuts at: 776 1650 776
Size: 874 3751
BanI G'GyrC_C
Cuts at: 220 1447 1641 2055 2303 2338 220
Size: 1227 194 414 248 35 2507
Fragments arranged by size:
2507 1227 414 248 194 35
BanII G_rGCy'C
Cuts at: 719 1020 1078 1288 1639 2669 719
Size: 301 58 210 351 1030 2675
Fragments arranged by size:
2675 1030 351 301 210 58
BbeI G_GCGC'C
Cuts at: 2059 2307 2059
Size: 248 4377
BbsI GAAGACnn'nnnn_
Cuts at: 688 688
Size: 4625
BbvCI CC'TCA_GC
Cuts at: 306 1042 306
Size: 736 3889
BciVI GTATCCnnnnn_n'
Cuts at: 168 2523 3284 4625 168
Size: 2355 761 1341 168
Fragments arranged by size:
2355 1341 761 168
BclI T'GATC_A
Cuts at: 1086 2145 1086
Size: 1059 3566
BglI GCCn_nnn'nGGC
Cuts at: 1283 1283
Size: 4625
BglII A'GATC_T
Cuts at: 1232 1481 2140 4177 1232
Size: 249 659 2037 1680
Fragments arranged by size:
2037 1680 659 249
BlpI GC'TnA_GC
Cuts at: 718 1737 718
Size: 1019 3606
Bme1580I G_kGCm'C
Cuts at: 536 1020 1176 1288 1471 2250 2343 3399 536
Size: 484 156 112 183 779 93 1056 1762
Fragments arranged by size:
1762 1056 779 484 183 156 112 93
BmrI ACTGGGnnnn_n'
Cuts at: 1104 1837 1918 2253 4397 1104
Size: 733 81 335 2144 1332
Fragments arranged by size:
2144 1332 733 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4625
Bpu10I CC'TnA_GC
Cuts at: 306 1042 1072 306
Size: 736 30 3859
Fragments arranged by size:
3859 736 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1588 1801 2680 3172 3470 1588
Size: 213 879 492 298 2743
Fragments arranged by size:
2743 879 492 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 1141 4329 1141
Size: 3188 1437
BsaAI yAC'GTr
Cuts at: 747 1978 747
Size: 1231 3394
BsaBI GATnn'nnATC
Cuts at: 6 1231 1486 2139 4176 6
Size: 1225 255 653 2037 455
Fragments arranged by size:
2037 1225 653 455 255
BsaHI Gr'CG_yC
Cuts at: 2056 2304 3006 4502 2056
Size: 248 702 1496 2179
Fragments arranged by size:
2179 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1814 2335 3013 3287 3434 3845 1814
Size: 521 678 274 147 411 2594
Fragments arranged by size:
2594 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 709 1068 1338 268
Size: 441 359 270 3555
Fragments arranged by size:
3555 441 359 270
BseYI C'CCAG_C
Cuts at: 2050 2808 3385 3881 2050
Size: 758 577 496 2794
Fragments arranged by size:
2794 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 352
Size: 4625
BsiEI CG_ry'CG
Cuts at: 104 2167 2213 3421 104
Size: 2063 46 1208 1308
Fragments arranged by size:
2063 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 719 1078 1130 1176 1639 2417 2607 3399
Size: 570 359 52 46 463 778 190 792
Cuts at: 3399 149
Size: 1375
Fragments arranged by size:
1375 792 778 570 463 359 190 52
46
BsmI GAATG_Cn'
Cuts at: 767 767
Size: 4625
BsmBI CGTCTCn'nnnn_
Cuts at: 469 469
Size: 4625
Bsp1286I G_dGCh'C
Cuts at: 149 536 719 1020 1078 1130 1176 1288 1471
Size: 387 183 301 58 52 46 112 183
Cuts at: 1471 1639 2250 2343 2417 2607 2669 3399 149
Size: 168 611 93 74 190 62 730 1375
Fragments arranged by size:
1375 730 611 387 301 190 183 183
168 112 93 74 62 58 52 46
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 723 1675 1889 2191 2572 723
Size: 952 214 302 381 2776
Fragments arranged by size:
2776 952 381 302 214
BsrBI CCG'CTC
Cuts at: 50 186 2917 2971 4455 4620 50
Size: 136 2731 54 1484 165 55
Fragments arranged by size:
2731 1484 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 206 455 2537 206
Size: 249 2082 2294
Fragments arranged by size:
2294 2082 249
BsrFI r'CCGG_y
Cuts at: 728 2623 2804 3013 728
Size: 1895 181 209 2340
Fragments arranged by size:
2340 1895 209 181
BsrGI T'GTAC_A
Cuts at: 1002 1002
Size: 4625
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 2002 2002
Size: 4625
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstEII G'GTnAC_C
Cuts at: 906 906
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 1548 1548
Size: 4625
BstYI r'GATC_y
Cuts at: 1 776 815 1232 1365 1481 1650 2117 2140
Size: 775 39 417 133 116 169 467 23
Cuts at: 2140 2475 2721 3733 3819 3831 4177 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 775 467 449 417 346 335 246
169 133 116 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 692 692
Size: 4625
BtgI C'CryG_G
Cuts at: 659 1214 659
Size: 555 4070
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1284 1560 2575 2756 2919 1284
Size: 276 1015 181 163 2990
Fragments arranged by size:
2990 1015 276 181 163
BtsI GCAGTG_nn'
Cuts at: 479 1868 479
Size: 1389 3236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 548 583 548
Size: 35 4590
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 1351 2331 3189 1351
Size: 980 858 2787
Fragments arranged by size:
2787 980 858
EaeI y'GGCC_r
Cuts at: 323 1262 1275 2210 2384 2775 2802 4147 323
Size: 939 13 935 174 391 27 1345 801
Fragments arranged by size:
1345 939 935 801 391 174 27 13
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 667 707 2648 2858 667
Size: 40 1941 210 2434
Fragments arranged by size:
2434 1941 210 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 847 2833 3143 3289 4435 847
Size: 1986 310 146 1146 1037
Fragments arranged by size:
1986 1146 1037 310 146
EcoICRI GAG'CTC
Cuts at: 717 1076 1637 717
Size: 359 561 3705
Fragments arranged by size:
3705 561 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2450 2882 3629 3897 2450
Size: 432 747 268 3178
Fragments arranged by size:
3178 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 936 1285 1764 936
Size: 349 479 3797
Fragments arranged by size:
3797 479 349
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4625
FspI TGC'GCA
Cuts at: 1372 2406 1372
Size: 1034 3591
HaeII r_GCGC'y
Cuts at: 2059 2307 3329 4308 2059
Size: 248 1022 979 2376
Fragments arranged by size:
2376 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1135 1167 1333 1365 1408 1440 2467 2497 2499
Size: 32 166 32 43 32 1027 30 2
Cuts at: 2499 2529 1135
Size: 30 3231
Fragments arranged by size:
3231 1027 166 43 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1664 1664
Size: 4625
HindIII A'AGCT_T
Cuts at: 335 631 1375 335
Size: 296 744 3585
Fragments arranged by size:
3585 744 296
KasI G'GCGC_C
Cuts at: 2055 2303 2055
Size: 248 4377
KpnI G_GTAC'C
Cuts at: 224 1451 1645 224
Size: 1227 194 3204
Fragments arranged by size:
3204 1227 194
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 827 3296 3480 827
Size: 2469 184 1972
Fragments arranged by size:
2469 1972 184
MscI TGG'CCA
Cuts at: 1264 1277 2386 1264
Size: 13 1109 3503
Fragments arranged by size:
3503 1109 13
MslI CAynn'nnrTG
Cuts at: 152 179 913 1025 1135 1546 2614 2741 152
Size: 27 734 112 110 411 1068 127 2036
Fragments arranged by size:
2036 1068 734 411 127 112 110 27
MspA1I CmG'CkG
Cuts at: 393 1336 1401 1736 2050 2410 3423 3668 393
Size: 943 65 335 314 360 1013 245 1350
Fragments arranged by size:
1350 1013 943 360 335 314 245 65
NaeI GCC'GGC
Cuts at: 2806 2806
Size: 4625
NarI GG'CG_CC
Cuts at: 2056 2304 2056
Size: 248 4377
NcoI C'CATG_G
Cuts at: 1214 1214
Size: 4625
NgoMIV G'CCGG_C
Cuts at: 2804 2804
Size: 4625
NspI r_CATG'y
Cuts at: 1678 2178 3085 1678
Size: 500 907 3218
Fragments arranged by size:
3218 907 500
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PflMI CCAn_nnn'nTGG
Cuts at: 520 939 520
Size: 419 4206
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PmlI CAC'GTG
Cuts at: 747 747
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 936 936
Size: 4625
PspOMI G'GGCC_C
Cuts at: 1016 1284 1016
Size: 268 4357
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 995 1027 995
Size: 32 4593
PstI C_TGCA'G
Cuts at: 398 1672 2357 398
Size: 1274 685 2666
Fragments arranged by size:
2666 1274 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 393 1401 2050 2410 393
Size: 1008 649 360 2608
Fragments arranged by size:
2608 1008 649 360
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 719 1078 1639 719
Size: 359 561 3705
Fragments arranged by size:
3705 561 359
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 707 2648 2858 707
Size: 1941 210 2474
Fragments arranged by size:
2474 1941 210
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 1093 4142 1093
Size: 3049 1576
SexAI A'CCwGG_T
Cuts at: 938 938
Size: 4625
SfcI C'TryA_G
Cuts at: 33 394 1331 1611 1668 2353 3346 3537 33
Size: 361 937 280 57 685 993 191 1121
Fragments arranged by size:
1121 993 937 685 361 280 191 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 1283 1283
Size: 4625
SfoI GGC'GCC
Cuts at: 2057 2305 2057
Size: 248 4377
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 1378 1603 1780 2695 3187 3449 1378
Size: 225 177 915 492 262 2554
Fragments arranged by size:
2554 915 492 262 225 177
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StuI AGG'CCT
Cuts at: 1040 1040
Size: 4625
StyI C'CwwG_G
Cuts at: 470 1133 1214 1759 2113 470
Size: 663 81 545 354 2982
Fragments arranged by size:
2982 663 545 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 164 299 559 583 972 2208 2341 2800 3009
Size: 135 260 24 389 1236 133 459 209
Cuts at: 3009 164
Size: 1780
Fragments arranged by size:
1780 1236 459 389 260 209 135 133
24
TatI w'GTAC_w
Cuts at: 203 1002 1091 4140 203
Size: 799 89 3049 688
Fragments arranged by size:
3049 799 688 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 4593
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2120
Size: 4625
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BpmI BsaXI BsiWI BssHII
BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PshAI PsiI PspXI SacII SanDI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI