Restriction map for
pF1KB5912
(Circular) (Six-base) MAPSORT of: pF1KB5912.seq Check: 8527 from: 1 to: 4700
pF1KB5912 4700 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 667 667
Size: 4700
AccI GT'mk_AC
Cuts at: 1738 1738
Size: 4700
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 1622 4540 1622
Size: 2918 1782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1664 2525 2957 3704 3972 271
Size: 1393 861 432 747 268 999
Fragments arranged by size:
1393 999 861 747 432 268
AflIII A'CryG_T
Cuts at: 2052 3156 4133 2052
Size: 1104 977 2619
Fragments arranged by size:
2619 1104 977
AgeI A'CCGG_T
Cuts at: 3088 3088
Size: 4700
AleI CACnn'nnGTG
Cuts at: 641 641
Size: 4700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 936 936
Size: 4700
AlwNI CAG_nnn'CTG
Cuts at: 249 754 2005 3572 249
Size: 505 1251 1567 1377
Fragments arranged by size:
1567 1377 1251 505
ApaLI G'TGCA_C
Cuts at: 3470 3470
Size: 4700
ApoI r'AATT_y
Cuts at: 475 1704 4053 4309 475
Size: 1229 2349 256 866
Fragments arranged by size:
2349 1229 866 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 1720 1720
Size: 4700
BamHI G'GATC_C
Cuts at: 1725 1725
Size: 4700
BanI G'GyrC_C
Cuts at: 592 1716 2130 2378 2413 592
Size: 1124 414 248 35 2879
Fragments arranged by size:
2879 1124 414 248 35
BanII G_rGCy'C
Cuts at: 663 1677 1714 2744 663
Size: 1014 37 1030 2619
Fragments arranged by size:
2619 1030 1014 37
BbeI G_GCGC'C
Cuts at: 2134 2382 2134
Size: 248 4452
BbsI GAAGACnn'nnnn_
Cuts at: 139 139
Size: 4700
BbvCI CC'TCA_GC
Cuts at: 220 220
Size: 4700
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3359 4700 2598
Size: 761 1341 2598
Fragments arranged by size:
2598 1341 761
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BglII A'GATC_T
Cuts at: 2215 4252 2215
Size: 2037 2663
BlpI GC'TnA_GC
Cuts at: 1812 1812
Size: 4700
Bme1580I G_kGCm'C
Cuts at: 325 537 995 2325 2418 3474 325
Size: 212 458 1330 93 1056 1551
Fragments arranged by size:
1551 1330 1056 458 212 93
BmrI ACTGGGnnnn_n'
Cuts at: 1912 1993 2328 4472 1912
Size: 81 335 2144 2140
Fragments arranged by size:
2144 2140 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
Bpu10I CC'TnA_GC
Cuts at: 220 220
Size: 4700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 1876 2755 3247 3545 383
Size: 1493 879 492 298 1538
Fragments arranged by size:
1538 1493 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4404 4404
Size: 4700
BsaAI yAC'GTr
Cuts at: 787 1175 2053 787
Size: 388 878 3434
Fragments arranged by size:
3434 878 388
BsaBI GATnn'nnATC
Cuts at: 6 2214 4251 6
Size: 2208 2037 455
Fragments arranged by size:
2208 2037 455
BsaHI Gr'CG_yC
Cuts at: 192 2131 2379 3081 4577 192
Size: 1939 248 702 1496 315
Fragments arranged by size:
1939 1496 702 315 248
BsaWI w'CCGG_w
Cuts at: 1889 2410 3088 3362 3509 3920 1889
Size: 521 678 274 147 411 2669
Fragments arranged by size:
2669 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 270 270
Size: 4700
BseYI C'CCAG_C
Cuts at: 600 923 1129 2125 2883 3460 3956 600
Size: 323 206 996 758 577 496 1344
Fragments arranged by size:
1344 996 758 577 496 323 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 740 818 740
Size: 78 4622
BsiEI CG_ry'CG
Cuts at: 104 1203 2242 2288 3496 104
Size: 1099 1039 46 1208 1308
Fragments arranged by size:
1308 1208 1099 1039 46
BsiHKAI G_wGCw'C
Cuts at: 1116 1714 2492 2682 3474 1116
Size: 598 778 190 792 2342
Fragments arranged by size:
2342 792 778 598 190
BsmI GAATG_Cn'
Cuts at: 129 604 129
Size: 475 4225
Bsp1286I G_dGCh'C
Cuts at: 325 537 663 995 1116 1677 1714 2325 2418
Size: 212 126 332 121 561 37 611 93
Cuts at: 2418 2492 2682 2744 3474 325
Size: 74 190 62 730 1551
Fragments arranged by size:
1551 730 611 561 332 212 190 126
121 93 74 62 37
BspEI T'CCGG_A
Cuts at: 3920 3920
Size: 4700
BspHI T'CATG_A
Cuts at: 891 3876 891
Size: 2985 1715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 667 928 1750 1964 2266 2647 667
Size: 261 822 214 302 381 2720
Fragments arranged by size:
2720 822 381 302 261 214
BsrBI CCG'CTC
Cuts at: 50 2992 3046 4530 4695 50
Size: 2942 54 1484 165 55
Fragments arranged by size:
2942 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 378 545 671 2612 378
Size: 167 126 1941 2466
Fragments arranged by size:
2466 1941 167 126
BsrFI r'CCGG_y
Cuts at: 1198 2698 2879 3088 1198
Size: 1500 181 209 2810
Fragments arranged by size:
2810 1500 209 181
BssSI C'ACGA_G
Cuts at: 2971 3329 2971
Size: 358 4342
BstAPI GCAn_nnn'nTGC
Cuts at: 2077 2077
Size: 4700
BstBI TT'CG_AA
Cuts at: 3061 3061
Size: 4700
BstXI CCAn_nnnn'nTGG
Cuts at: 579 579
Size: 4700
BstYI r'GATC_y
Cuts at: 1 1725 2192 2215 2550 2796 3808 3894 3906
Size: 1724 467 23 335 246 1012 86 12
Cuts at: 3906 4252 1
Size: 346 449
Fragments arranged by size:
1724 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2650 2831 2994 2650
Size: 181 163 4356
Fragments arranged by size:
4356 181 163
BtsI GCAGTG_nn'
Cuts at: 1943 1943
Size: 4700
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 2406 3264 2406
Size: 858 3842
EaeI y'GGCC_r
Cuts at: 1200 2285 2459 2850 2877 4222 1200
Size: 1085 174 391 27 1345 1678
Fragments arranged by size:
1678 1345 1085 391 174 27
EagI C'GGCC_G
Cuts at: 1200 2285 1200
Size: 1085 3615
EarI CTCTTCn'nnn_
Cuts at: 247 1601 2723 2933 247
Size: 1354 1122 210 2014
Fragments arranged by size:
2014 1354 1122 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3218 3364 4510 2908
Size: 310 146 1146 3098
Fragments arranged by size:
3098 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1712 1712
Size: 4700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1664 2525 2957 3704 3972 271
Size: 1393 861 432 747 268 999
Fragments arranged by size:
1393 999 861 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1682 1839 1682
Size: 157 4543
EcoRI G'AATT_C
Cuts at: 1704 1704
Size: 4700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4700
FspI TGC'GCA
Cuts at: 2481 2481
Size: 4700
HaeII r_GCGC'y
Cuts at: 2134 2382 3404 4383 2134
Size: 248 1022 979 2451
Fragments arranged by size:
2451 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 581 613 748 780 2542 2572 2574 2604 581
Size: 32 135 32 1762 30 2 30 2677
Fragments arranged by size:
2677 1762 135 32 32 30 30 2
HincII GTy'rAC
Cuts at: 726 1739 726
Size: 1013 3687
HindIII A'AGCT_T
Cuts at: 1006 1575 1006
Size: 569 4131
KasI G'GCGC_C
Cuts at: 2130 2378 2130
Size: 248 4452
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MfeI C'AATT_G
Cuts at: 1031 1031
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3371 3555 3371
Size: 184 4516
MscI TGG'CCA
Cuts at: 2461 2461
Size: 4700
MslI CAynn'nnrTG
Cuts at: 641 893 973 1547 2689 2816 641
Size: 252 80 574 1142 127 2525
Fragments arranged by size:
2525 1142 574 252 127 80
MspA1I CmG'CkG
Cuts at: 139 297 1811 2125 2485 3498 3743 139
Size: 158 1514 314 360 1013 245 1096
Fragments arranged by size:
1514 1096 1013 360 314 245 158
NaeI GCC'GGC
Cuts at: 1200 2881 1200
Size: 1681 3019
NarI GG'CG_CC
Cuts at: 2131 2379 2131
Size: 248 4452
NgoMIV G'CCGG_C
Cuts at: 1198 2879 1198
Size: 1681 3019
NspI r_CATG'y
Cuts at: 776 1753 2253 3160 776
Size: 977 500 907 2316
Fragments arranged by size:
2316 977 907 500
PciI A'CATG_T
Cuts at: 3156 3156
Size: 4700
PfoI T'CCnGG_A
Cuts at: 1427 1427
Size: 4700
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 4668
PpuMI rG'GwC_Cy
Cuts at: 1682 1682
Size: 4700
PstI C_TGCA'G
Cuts at: 302 1747 2432 302
Size: 1445 685 2570
Fragments arranged by size:
2570 1445 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 139 2125 2485 139
Size: 1986 360 2354
Fragments arranged by size:
2354 1986 360
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1714 1714
Size: 4700
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 2723 2933 2723
Size: 210 4490
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 4217 4217
Size: 4700
SfcI C'TryA_G
Cuts at: 33 298 1598 1743 2428 3421 3612 33
Size: 265 1300 145 685 993 191 1121
Fragments arranged by size:
1300 1121 993 685 265 191 145
SfoI GGC'GCC
Cuts at: 2132 2380 2132
Size: 248 4452
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 1722 1722
Size: 4700
SmlI C'TyrA_G
Cuts at: 398 1855 2770 3262 3524 398
Size: 1457 915 492 262 1574
Fragments arranged by size:
1574 1457 915 492 262
SnaBI TAC'GTA
Cuts at: 787 787
Size: 4700
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1753 2253 1753
Size: 500 4200
SspI AAT'ATT
Cuts at: 1065 1065
Size: 4700
StyI C'CwwG_G
Cuts at: 572 1834 2188 572
Size: 1262 354 3084
Fragments arranged by size:
3084 1262 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2283 2416 2875 3084 2283
Size: 133 459 209 3899
Fragments arranged by size:
3899 459 209 133
TatI w'GTAC_w
Cuts at: 178 4215 178
Size: 4037 663
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1159 1191 1285 1317 4217 4249 1159
Size: 32 94 32 2900 32 1610
Fragments arranged by size:
2900 1610 94 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 349 1182 2497 349
Size: 833 1315 2552
Fragments arranged by size:
2552 1315 833
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1416 1666 2195 1416
Size: 250 529 3921
Fragments arranged by size:
3921 529 250
XmaI C'CCGG_G
Cuts at: 1720 1720
Size: 4700
XmnI GAAnn'nnTTC
Cuts at: 1591 1591
Size: 4700
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI XhoI
ZraI