Restriction map for
pF1KB5913
(Circular) (Six-base) MAPSORT of: pF1KB5913.seq Check: 4560 from: 1 to: 4625
pF1KB5913 4625 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 857 857
Size: 4625
AatII G_ACGT'C
Cuts at: 647 647
Size: 4625
AccI GT'mk_AC
Cuts at: 557 1663 557
Size: 1106 3519
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2450 2882 3629 3897 1192
Size: 1258 432 747 268 1920
Fragments arranged by size:
1920 1258 747 432 268
AflII C'TTAA_G
Cuts at: 1525 1525
Size: 4625
AflIII A'CryG_T
Cuts at: 635 1977 3081 4058 635
Size: 1342 1104 977 1202
Fragments arranged by size:
1342 1202 1104 977
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AleI CACnn'nnGTG
Cuts at: 872 872
Size: 4625
AlwNI CAG_nnn'CTG
Cuts at: 233 1114 1170 1551 1930 3497 233
Size: 881 56 381 379 1567 1361
Fragments arranged by size:
1567 1361 881 381 379 56
ApaI G_GGCC'C
Cuts at: 1087 1392 1087
Size: 305 4320
ApaLI G'TGCA_C
Cuts at: 3395 3395
Size: 4625
ApoI r'AATT_y
Cuts at: 1629 3978 4234 1629
Size: 2349 256 2020
Fragments arranged by size:
2349 2020 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 1181 1289 1645 1181
Size: 108 356 4161
Fragments arranged by size:
4161 356 108
BamHI G'GATC_C
Cuts at: 495 1650 495
Size: 1155 3470
BanI G'GyrC_C
Cuts at: 778 1641 2055 2303 2338 778
Size: 863 414 248 35 3065
Fragments arranged by size:
3065 863 414 248 35
BanII G_rGCy'C
Cuts at: 186 326 974 1087 1290 1392 1639 2669 186
Size: 140 648 113 203 102 247 1030 2142
Fragments arranged by size:
2142 1030 648 247 203 140 113 102
BbeI G_GCGC'C
Cuts at: 2059 2307 2059
Size: 248 4377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4591
BciVI GTATCCnnnnn_n'
Cuts at: 1326 2523 3284 4625 1326
Size: 1197 761 1341 1326
Fragments arranged by size:
1341 1326 1197 761
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BglI GCCn_nnn'nGGC
Cuts at: 181 550 181
Size: 369 4256
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 185 1295 1737 185
Size: 1110 442 3073
Fragments arranged by size:
3073 1110 442
Bme1580I G_kGCm'C
Cuts at: 1087 1392 2250 2343 3399 1087
Size: 305 858 93 1056 2313
Fragments arranged by size:
2313 1056 858 305 93
BmgBI CAC'GTC
Cuts at: 665 665
Size: 4625
BmrI ACTGGGnnnn_n'
Cuts at: 224 1837 1918 2253 4397 224
Size: 1613 81 335 2144 452
Fragments arranged by size:
2144 1613 452 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 382 1367 1486 340
Size: 42 985 119 3479
Fragments arranged by size:
3479 985 119 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1801 2680 3172 3470 1801
Size: 879 492 298 2956
Fragments arranged by size:
2956 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1343 4329 1343
Size: 2986 1639
BsaAI yAC'GTr
Cuts at: 1978 1978
Size: 4625
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 644 2056 2304 3006 4502 644
Size: 1412 248 702 1496 767
Fragments arranged by size:
1496 1412 767 702 248
BsaWI w'CCGG_w
Cuts at: 584 983 1430 1814 2335 3013 3287 3434 3845
Size: 399 447 384 521 678 274 147 411
Cuts at: 3845 584
Size: 1364
Fragments arranged by size:
1364 678 521 447 411 399 384 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 238 268 422 452 503 533 238
Size: 30 154 30 51 30 4330
Fragments arranged by size:
4330 154 51 30 30 30
BseYI C'CCAG_C
Cuts at: 540 1392 2050 2808 3385 3881 540
Size: 852 658 758 577 496 1284
Fragments arranged by size:
1284 852 758 658 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 1119 485
Size: 634 3991
BsiEI CG_ry'CG
Cuts at: 104 585 2167 2213 3421 104
Size: 481 1582 46 1208 1308
Fragments arranged by size:
1582 1308 1208 481 46
BsiHKAI G_wGCw'C
Cuts at: 326 1290 1639 2417 2607 3399 326
Size: 964 349 778 190 792 1552
Fragments arranged by size:
1552 964 792 778 349 190
BsmI GAATG_Cn'
Cuts at: 1182 1182
Size: 4625
Bsp1286I G_dGCh'C
Cuts at: 186 326 974 1087 1290 1392 1639 2250 2343
Size: 140 648 113 203 102 247 611 93
Cuts at: 2343 2417 2607 2669 3399 186
Size: 74 190 62 730 1412
Fragments arranged by size:
1412 730 648 611 247 203 190 140
113 102 93 74 62
BspEI T'CCGG_A
Cuts at: 1430 3845 1430
Size: 2415 2210
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 611 857 1155 1271 1675 1889 2191 2572 611
Size: 246 298 116 404 214 302 381 2664
Fragments arranged by size:
2664 404 381 302 298 246 214 116
BsrBI CCG'CTC
Cuts at: 50 1299 2917 2971 4455 4620 50
Size: 1249 1618 54 1484 165 55
Fragments arranged by size:
1618 1484 1249 165 55 54
BsrDI GCAATG_nn'
Cuts at: 326 1100 2537 326
Size: 774 1437 2414
Fragments arranged by size:
2414 1437 774
BsrFI r'CCGG_y
Cuts at: 2623 2804 3013 2623
Size: 181 209 4235
Fragments arranged by size:
4235 209 181
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 742 2002 742
Size: 1260 3365
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 874 1278 874
Size: 404 4221
BstYI r'GATC_y
Cuts at: 1 495 1025 1650 2117 2140 2475 2721 3733
Size: 494 530 625 467 23 335 246 1012
Cuts at: 3733 3819 3831 4177 1
Size: 86 12 346 449
Fragments arranged by size:
1012 625 530 494 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1561 1561
Size: 4625
BtgI C'CryG_G
Cuts at: 627 760 1187 627
Size: 133 427 4065
Fragments arranged by size:
4065 427 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 313 1322 1868 313
Size: 1009 546 3070
Fragments arranged by size:
3070 1009 546
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DraIII CAC_nnn'GTG
Cuts at: 1251 1251
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 173 551 757 1017 1553 2210 2384 2775 2802
Size: 378 206 260 536 657 174 391 27
Cuts at: 2802 4147 173
Size: 1345 651
Fragments arranged by size:
1345 657 651 536 391 378 260 206
174 27
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 956 1477 2648 2858 956
Size: 521 1171 210 2723
Fragments arranged by size:
2723 1171 521 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2833 3143 3289 4435 2833
Size: 310 146 1146 3023
Fragments arranged by size:
3023 1146 310 146
EcoICRI GAG'CTC
Cuts at: 324 1288 1637 324
Size: 964 349 3312
Fragments arranged by size:
3312 964 349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 382 1192 1367 1486 2450 2882 3629 3897
Size: 42 810 175 119 964 432 747 268
Cuts at: 3897 340
Size: 1068
Fragments arranged by size:
1068 964 810 747 432 268 175 119
42
EcoNI CCTnn'n_nnAGG
Cuts at: 1126 1525 1126
Size: 399 4226
EcoO109I rG'GnC_Cy
Cuts at: 366 374 536 1083 1084 1275 1388 1389 1589
Size: 8 162 547 1 191 113 1 200
Cuts at: 1589 1764 366
Size: 175 3227
Fragments arranged by size:
3227 547 200 191 175 162 113 8
1 1
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
EcoRV GAT'ATC
Cuts at: 656 656
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4625
FspI TGC'GCA
Cuts at: 2406 2406
Size: 4625
HaeII r_GCGC'y
Cuts at: 471 788 2059 2307 3329 4308 471
Size: 317 1271 248 1022 979 788
Fragments arranged by size:
1271 1022 979 788 317 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 503 535 1300 1332 1498 1530 2467
Size: 32 233 32 765 32 166 32 937
Cuts at: 2467 2497 2499 2529 238
Size: 30 2 30 2334
Fragments arranged by size:
2334 937 765 233 166 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1464 1664 1464
Size: 200 4425
KasI G'GCGC_C
Cuts at: 2055 2303 2055
Size: 248 4377
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 236 3296 3480 236
Size: 3060 184 1381
Fragments arranged by size:
3060 1381 184
MscI TGG'CCA
Cuts at: 175 1555 2386 175
Size: 1380 831 2414
Fragments arranged by size:
2414 1380 831
MslI CAynn'nnrTG
Cuts at: 872 1094 1141 2614 2741 872
Size: 222 47 1473 127 2756
Fragments arranged by size:
2756 1473 222 127 47
MspA1I CmG'CkG
Cuts at: 162 732 1736 2050 2410 3423 3668 162
Size: 570 1004 314 360 1013 245 1119
Fragments arranged by size:
1119 1013 1004 570 360 314 245
NaeI GCC'GGC
Cuts at: 2806 2806
Size: 4625
NarI GG'CG_CC
Cuts at: 2056 2304 2056
Size: 248 4377
NcoI C'CATG_G
Cuts at: 1187 1187
Size: 4625
NgoMIV G'CCGG_C
Cuts at: 2804 2804
Size: 4625
NspI r_CATG'y
Cuts at: 639 1621 1678 2178 3085 639
Size: 982 57 500 907 2179
Fragments arranged by size:
2179 982 907 500 57
PasI CC'CwG_GG
Cuts at: 369 369
Size: 4625
PciI A'CATG_T
Cuts at: 635 3081 635
Size: 2446 2179
PfoI T'CCnGG_A
Cuts at: 1148 1344 1502 1148
Size: 196 158 4271
Fragments arranged by size:
4271 196 158
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 374 536 374
Size: 162 4463
PspOMI G'GGCC_C
Cuts at: 1083 1388 1083
Size: 305 4320
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 376 408 376
Size: 32 4593
PstI C_TGCA'G
Cuts at: 149 290 349 1079 1110 1166 1672 2357 149
Size: 141 59 730 31 56 506 685 2417
Fragments arranged by size:
2417 730 685 506 141 59 56 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 162 732 2050 2410 162
Size: 570 1318 360 2377
Fragments arranged by size:
2377 1318 570 360
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 326 1290 1639 326
Size: 964 349 3312
Fragments arranged by size:
3312 964 349
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SanDI GG'GwC_CC
Cuts at: 536 536
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 1477 2648 2858 1477
Size: 1171 210 3244
Fragments arranged by size:
3244 1171 210
SbfI CC_TGCA'GG
Cuts at: 1079 1166 1672 1079
Size: 87 506 4032
Fragments arranged by size:
4032 506 87
ScaI AGT'ACT
Cuts at: 394 1156 4142 394
Size: 762 2986 877
Fragments arranged by size:
2986 877 762
SexAI A'CCwGG_T
Cuts at: 1247 1247
Size: 4625
SfcI C'TryA_G
Cuts at: 33 145 286 345 1075 1106 1162 1668 2353
Size: 112 141 59 730 31 56 506 685
Cuts at: 2353 3346 3537 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 730 685 506 191 141 112
59 56 31
SfoI GGC'GCC
Cuts at: 2057 2305 2057
Size: 248 4377
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 1525 1780 2695 3187 3449 1525
Size: 255 915 492 262 2701
Fragments arranged by size:
2701 915 492 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StuI AGG'CCT
Cuts at: 1132 1132
Size: 4625
StyI C'CwwG_G
Cuts at: 476 890 1187 1271 1759 2113 476
Size: 414 297 84 488 354 2988
Fragments arranged by size:
2988 488 414 354 297 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 640 1061 1367 2208 2341 2800 3009 640
Size: 421 306 841 133 459 209 2256
Fragments arranged by size:
2256 841 459 421 306 209 133
TatI w'GTAC_w
Cuts at: 113 392 1154 4140 113
Size: 279 762 2986 598
Fragments arranged by size:
2986 762 598 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 292 324 422 454 4142 4174 292
Size: 32 98 32 3688 32 743
Fragments arranged by size:
3688 743 98 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 925 2422 925
Size: 1497 3128
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 211 2120 211
Size: 1909 2716
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
ZraI GAC'GTC
Cuts at: 645 645
Size: 4625
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI AscI AvrII BaeI BbsI
BbvCI BfrBI BmtI Bpu10I BseRI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI