Restriction map for
pF1KB5914
(Circular) (Six-base) MAPSORT of: pF1KB5914.seq Check: 3443 from: 1 to: 4703
pF1KB5914 4703 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 218 1741 218
Size: 1523 3180
Acc65I G'GTAC_C
Cuts at: 782 1719 782
Size: 937 3766
AclI AA'CG_TT
Cuts at: 4543 4543
Size: 4703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2528 2960 3707 3975 2528
Size: 432 747 268 3256
Fragments arranged by size:
3256 747 432 268
AflIII A'CryG_T
Cuts at: 2055 3159 4136 2055
Size: 1104 977 2622
Fragments arranged by size:
2622 1104 977
AgeI A'CCGG_T
Cuts at: 3091 3091
Size: 4703
AlwNI CAG_nnn'CTG
Cuts at: 2008 3575 2008
Size: 1567 3136
ApaLI G'TGCA_C
Cuts at: 3473 3473
Size: 4703
ApoI r'AATT_y
Cuts at: 1223 1707 4056 4312 1223
Size: 484 2349 256 1614
Fragments arranged by size:
2349 1614 484 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4703
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
AvaI C'yCGr_G
Cuts at: 866 901 1723 866
Size: 35 822 3846
Fragments arranged by size:
3846 822 35
BamHI G'GATC_C
Cuts at: 1728 1728
Size: 4703
BanI G'GyrC_C
Cuts at: 359 782 1719 2133 2381 2416 359
Size: 423 937 414 248 35 2646
Fragments arranged by size:
2646 937 423 414 248 35
BanII G_rGCy'C
Cuts at: 901 1073 1678 1717 2747 901
Size: 172 605 39 1030 2857
Fragments arranged by size:
2857 1030 605 172 39
BbeI G_GCGC'C
Cuts at: 2137 2385 2137
Size: 248 4455
BciVI GTATCCnnnnn_n'
Cuts at: 2601 3362 4703 2601
Size: 761 1341 2601
Fragments arranged by size:
2601 1341 761
BclI T'GATC_A
Cuts at: 950 1034 2223 950
Size: 84 1189 3430
Fragments arranged by size:
3430 1189 84
BglI GCCn_nnn'nGGC
Cuts at: 318 838 318
Size: 520 4183
BglII A'GATC_T
Cuts at: 1182 2218 4255 1182
Size: 1036 2037 1630
Fragments arranged by size:
2037 1630 1036
BlpI GC'TnA_GC
Cuts at: 932 1072 1815 932
Size: 140 743 3820
Fragments arranged by size:
3820 743 140
Bme1580I G_kGCm'C
Cuts at: 362 2328 2421 3477 362
Size: 1966 93 1056 1588
Fragments arranged by size:
1966 1588 1056 93
BmgBI CAC'GTC
Cuts at: 1500 1500
Size: 4703
BmrI ACTGGGnnnn_n'
Cuts at: 956 1558 1915 1996 2331 4475 956
Size: 602 357 81 335 2144 1184
Fragments arranged by size:
2144 1184 602 357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 207 560 3070 207
Size: 353 2510 1840
Fragments arranged by size:
2510 1840 353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 946 1163 1603 202
Size: 744 217 440 3302
Fragments arranged by size:
3302 744 440 217
Bpu10I CC'TnA_GC
Cuts at: 895 895
Size: 4703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1879 2758 3250 3548 1879
Size: 879 492 298 3034
Fragments arranged by size:
3034 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4407 4407
Size: 4703
BsaAI yAC'GTr
Cuts at: 1602 2056 1602
Size: 454 4249
BsaBI GATnn'nnATC
Cuts at: 6 2217 4254 6
Size: 2211 2037 455
Fragments arranged by size:
2211 2037 455
BsaHI Gr'CG_yC
Cuts at: 890 2134 2382 3084 4580 890
Size: 1244 248 702 1496 1013
Fragments arranged by size:
1496 1244 1013 702 248
BsaWI w'CCGG_w
Cuts at: 1892 2413 3091 3365 3512 3923 1892
Size: 521 678 274 147 411 2672
Fragments arranged by size:
2672 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1437 1437
Size: 4703
BseYI C'CCAG_C
Cuts at: 283 1040 1451 2128 2886 3463 3959 283
Size: 757 411 677 758 577 496 1027
Fragments arranged by size:
1027 758 757 677 577 496 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1662 808
Size: 854 3849
BsiEI CG_ry'CG
Cuts at: 104 2245 2291 3499 104
Size: 2141 46 1208 1308
Fragments arranged by size:
2141 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 188 917 1073 1717 2495 2685 3477 188
Size: 729 156 644 778 190 792 1414
Fragments arranged by size:
1414 792 778 729 644 190 156
BsmI GAATG_Cn'
Cuts at: 278 635 1599 278
Size: 357 964 3382
Fragments arranged by size:
3382 964 357
Bsp1286I G_dGCh'C
Cuts at: 188 362 901 917 1073 1678 1717 2328 2421
Size: 174 539 16 156 605 39 611 93
Cuts at: 2421 2495 2685 2747 3477 188
Size: 74 190 62 730 1414
Fragments arranged by size:
1414 730 611 605 539 190 174 156
93 74 62 39 16
BspEI T'CCGG_A
Cuts at: 3923 3923
Size: 4703
BspHI T'CATG_A
Cuts at: 3879 3879
Size: 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1583 1753 1967 2269 2650 1583
Size: 170 214 302 381 3636
Fragments arranged by size:
3636 381 302 214 170
BsrBI CCG'CTC
Cuts at: 50 1439 2995 3049 4533 4698 50
Size: 1389 1556 54 1484 165 55
Fragments arranged by size:
1556 1484 1389 165 55 54
BsrDI GCAATG_nn'
Cuts at: 294 2615 294
Size: 2321 2382
BsrFI r'CCGG_y
Cuts at: 695 832 2701 2882 3091 695
Size: 137 1869 181 209 2307
Fragments arranged by size:
2307 1869 209 181 137
BsrGI T'GTAC_A
Cuts at: 679 679
Size: 4703
BssSI C'ACGA_G
Cuts at: 2974 3332 2974
Size: 358 4345
BstAPI GCAn_nnn'nTGC
Cuts at: 707 912 1464 2080 707
Size: 205 552 616 3330
Fragments arranged by size:
3330 616 552 205
BstBI TT'CG_AA
Cuts at: 3064 3064
Size: 4703
BstXI CCAn_nnnn'nTGG
Cuts at: 1470 1470
Size: 4703
BstYI r'GATC_y
Cuts at: 1 1182 1728 2195 2218 2553 2799 3811 3897
Size: 1181 546 467 23 335 246 1012 86
Cuts at: 3897 3909 4255 1
Size: 12 346 449
Fragments arranged by size:
1181 1012 546 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4703
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 367 2653 2834 2997 367
Size: 2286 181 163 2073
Fragments arranged by size:
2286 2073 181 163
BtsI GCAGTG_nn'
Cuts at: 1946 1946
Size: 4703
ClaI AT'CG_AT
Cuts at: 686 686
Size: 4703
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 831 866 831
Size: 35 4668
DraI TTT'AAA
Cuts at: 627 1702 627
Size: 1075 3628
DrdI GACnn_nn'nnGTC
Cuts at: 2409 3267 2409
Size: 858 3845
EaeI y'GGCC_r
Cuts at: 346 855 1010 2288 2462 2853 2880 4225 346
Size: 509 155 1278 174 391 27 1345 824
Fragments arranged by size:
1345 1278 824 509 391 174 155 27
EagI C'GGCC_G
Cuts at: 2288 2288
Size: 4703
EarI CTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2911 3221 3367 4513 2911
Size: 310 146 1146 3101
Fragments arranged by size:
3101 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1071 1715 1071
Size: 644 4059
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 946 1163 1603 2528 2960 3707 3975 202
Size: 744 217 440 925 432 747 268 930
Fragments arranged by size:
930 925 747 744 440 432 268 217
EcoNI CCTnn'n_nnAGG
Cuts at: 925 925
Size: 4703
EcoO109I rG'GnC_Cy
Cuts at: 1842 1842
Size: 4703
EcoRI G'AATT_C
Cuts at: 1223 1707 1223
Size: 484 4219
EcoRV GAT'ATC
Cuts at: 583 583
Size: 4703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1188 2722 1188
Size: 1534 3169
FspI TGC'GCA
Cuts at: 2484 2484
Size: 4703
HaeII r_GCGC'y
Cuts at: 1146 2137 2385 3407 4386 1146
Size: 991 248 1022 979 1463
Fragments arranged by size:
1463 1022 991 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 559 591 2545 2575 2577 2607 559
Size: 32 1954 30 2 30 2655
Fragments arranged by size:
2655 1954 32 30 30 2
HincII GTy'rAC
Cuts at: 1742 1742
Size: 4703
KasI G'GCGC_C
Cuts at: 2133 2381 2133
Size: 248 4455
KpnI G_GTAC'C
Cuts at: 786 1723 786
Size: 937 3766
MfeI C'AATT_G
Cuts at: 1148 1148
Size: 4703
MluI A'CGCG_T
Cuts at: 4136 4136
Size: 4703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1308 3374 3558 1090
Size: 218 2066 184 2235
Fragments arranged by size:
2235 2066 218 184
MscI TGG'CCA
Cuts at: 348 2464 348
Size: 2116 2587
MslI CAynn'nnrTG
Cuts at: 401 991 2692 2819 401
Size: 590 1701 127 2285
Fragments arranged by size:
2285 1701 590 127
MspA1I CmG'CkG
Cuts at: 822 1040 1814 2128 2488 3501 3746 822
Size: 218 774 314 360 1013 245 1779
Fragments arranged by size:
1779 1013 774 360 314 245 218
NaeI GCC'GGC
Cuts at: 834 2884 834
Size: 2050 2653
NarI GG'CG_CC
Cuts at: 2134 2382 2134
Size: 248 4455
NcoI C'CATG_G
Cuts at: 107 107
Size: 4703
NgoMIV G'CCGG_C
Cuts at: 832 2882 832
Size: 2050 2653
NspI r_CATG'y
Cuts at: 1245 1510 1756 2256 3163 1245
Size: 265 246 500 907 2785
Fragments arranged by size:
2785 907 500 265 246
PciI A'CATG_T
Cuts at: 3159 3159
Size: 4703
PflMI CCAn_nnn'nTGG
Cuts at: 355 355
Size: 4703
PfoI T'CCnGG_A
Cuts at: 763 923 763
Size: 160 4543
PmeI GTTT'AAAC
Cuts at: 1702 1702
Size: 4703
PmlI CAC'GTG
Cuts at: 1602 1602
Size: 4703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1520 1552 3842 3874 1520
Size: 32 2290 32 2349
Fragments arranged by size:
2349 2290 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 440 472 440
Size: 32 4671
PstI C_TGCA'G
Cuts at: 163 565 1750 2435 163
Size: 402 1185 685 2431
Fragments arranged by size:
2431 1185 685 402
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4703
PvuII CAG'CTG
Cuts at: 822 1040 2128 2488 822
Size: 218 1088 360 3037
Fragments arranged by size:
3037 1088 360 218
RsrII CG'GwC_CG
Cuts at: 2898 2898
Size: 4703
SacI G_AGCT'C
Cuts at: 1073 1717 1073
Size: 644 4059
SalI G'TCGA_C
Cuts at: 1740 1740
Size: 4703
SapI GCTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
SbfI CC_TGCA'GG
Cuts at: 1750 1750
Size: 4703
ScaI AGT'ACT
Cuts at: 4220 4220
Size: 4703
SfcI C'TryA_G
Cuts at: 33 159 252 561 1746 2431 3424 3615 33
Size: 126 93 309 1185 685 993 191 1121
Fragments arranged by size:
1185 1121 993 685 309 191 126 93
SfoI GGC'GCC
Cuts at: 2135 2383 2135
Size: 248 4455
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
SmaI CCC'GGG
Cuts at: 1725 1725
Size: 4703
SmlI C'TyrA_G
Cuts at: 1858 2773 3265 3527 1858
Size: 915 492 262 3034
Fragments arranged by size:
3034 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4703
SphI G_CATG'C
Cuts at: 1510 1756 2256 1510
Size: 246 500 3957
Fragments arranged by size:
3957 500 246
StuI AGG'CCT
Cuts at: 619 715 982 619
Size: 96 267 4340
Fragments arranged by size:
4340 267 96
StyI C'CwwG_G
Cuts at: 107 1837 2191 107
Size: 1730 354 2619
Fragments arranged by size:
2619 1730 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 1423 2286 2419 2878 3087 1413
Size: 10 863 133 459 209 3029
Fragments arranged by size:
3029 863 459 209 133 10
TatI w'GTAC_w
Cuts at: 679 4218 679
Size: 3539 1164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 477 509 4220 4252 477
Size: 32 3711 32 928
Fragments arranged by size:
3711 928 32 32
Tth111I GACn'n_nGTC
Cuts at: 2500 2500
Size: 4703
XbaI T'CTAG_A
Cuts at: 1734 1734
Size: 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 341 2198 177
Size: 164 1857 2682
Fragments arranged by size:
2682 1857 164
XmaI C'CCGG_G
Cuts at: 1723 1723
Size: 4703
XmnI GAAnn'nnTTC
Cuts at: 1223 1223
Size: 4703
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
DraIII FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI