Restriction map for
pF1KB5915
(Circular) (Six-base) MAPSORT of: pF1KB5915.seq Check: 7378 from: 1 to: 4628
pF1KB5915 4628 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 510 510
Size: 4628
AatII G_ACGT'C
Cuts at: 1244 1481 1244
Size: 237 4391
AccI GT'mk_AC
Cuts at: 1489 1666 1489
Size: 177 4451
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 1046 4468 1046
Size: 3422 1206
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 2453 2885 3632 3900 670
Size: 1783 432 747 268 1398
Fragments arranged by size:
1783 1398 747 432 268
AflIII A'CryG_T
Cuts at: 1214 1234 1409 1980 3084 4061 1214
Size: 20 175 571 1104 977 1781
Fragments arranged by size:
1781 1104 977 571 175 20
AgeI A'CCGG_T
Cuts at: 3016 3016
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 267 468 660 1933 3500 267
Size: 201 192 1273 1567 1395
Fragments arranged by size:
1567 1395 1273 201 192
ApaI G_GGCC'C
Cuts at: 330 352 1322 330
Size: 22 970 3636
Fragments arranged by size:
3636 970 22
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 1080 1142 1632 3981 4237 1080
Size: 62 490 2349 256 1471
Fragments arranged by size:
2349 1471 490 256 62
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 703 1063 1648 703
Size: 360 585 3683
Fragments arranged by size:
3683 585 360
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 1089 1644 2058 2306 2341 1089
Size: 555 414 248 35 3376
Fragments arranged by size:
3376 555 414 248 35
BanII G_rGCy'C
Cuts at: 330 352 755 1322 1642 2672 330
Size: 22 403 567 320 1030 2286
Fragments arranged by size:
2286 1030 567 403 320 22
BbeI G_GCGC'C
Cuts at: 1093 2062 2310 1093
Size: 969 248 3411
Fragments arranged by size:
3411 969 248
BbvCI CC'TCA_GC
Cuts at: 277 661 277
Size: 384 4244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1054 1088 1054
Size: 34 4594
BciVI GTATCCnnnnn_n'
Cuts at: 1153 2526 3287 4628 1153
Size: 1373 761 1341 1153
Fragments arranged by size:
1373 1341 1153 761
BclI T'GATC_A
Cuts at: 2148 2148
Size: 4628
BglI GCCn_nnn'nGGC
Cuts at: 112 325 112
Size: 213 4415
BglII A'GATC_T
Cuts at: 908 2143 4180 908
Size: 1235 2037 1356
Fragments arranged by size:
2037 1356 1235
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 330 352 1322 2253 2346 3402 330
Size: 22 970 931 93 1056 1556
Fragments arranged by size:
1556 1056 970 931 93 22
BmgBI CAC'GTC
Cuts at: 452 1412 452
Size: 960 3668
BmrI ACTGGGnnnn_n'
Cuts at: 594 689 1840 1921 2256 4400 594
Size: 95 1151 81 335 2144 822
Fragments arranged by size:
2144 1151 822 335 95 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 450 1531 2995 450
Size: 1081 1464 2083
Fragments arranged by size:
2083 1464 1081
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 520 617 937 160
Size: 360 97 320 3851
Fragments arranged by size:
3851 360 320 97
Bpu10I CC'TnA_GC
Cuts at: 232 277 661 766 232
Size: 45 384 105 4094
Fragments arranged by size:
4094 384 105 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 1501 1804 2683 3175 3473 1384
Size: 117 303 879 492 298 2539
Fragments arranged by size:
2539 879 492 303 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 623 1166 1273 4332 623
Size: 543 107 3059 919
Fragments arranged by size:
3059 919 543 107
BsaAI yAC'GTr
Cuts at: 1981 1981
Size: 4628
BsaBI GATnn'nnATC
Cuts at: 6 2142 4179 6
Size: 2136 2037 455
Fragments arranged by size:
2136 2037 455
BsaHI Gr'CG_yC
Cuts at: 1090 1241 1478 2059 2307 3009 4505 1090
Size: 151 237 581 248 702 1496 1213
Fragments arranged by size:
1496 1213 702 581 248 237 151
BsaWI w'CCGG_w
Cuts at: 1175 1817 2338 3016 3290 3437 3848 1175
Size: 642 521 678 274 147 411 1955
Fragments arranged by size:
1955 678 642 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 669 699 742 772 1396 1426 669
Size: 30 43 30 624 30 3871
Fragments arranged by size:
3871 624 43 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 403 611 790 133
Size: 270 208 179 3971
Fragments arranged by size:
3971 270 208 179
BseYI C'CCAG_C
Cuts at: 934 1314 2053 2811 3388 3884 934
Size: 380 739 758 577 496 1678
Fragments arranged by size:
1678 758 739 577 496 380
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 280 1381 148
Size: 132 1101 3395
Fragments arranged by size:
3395 1101 132
BsiEI CG_ry'CG
Cuts at: 104 116 2170 2216 3424 104
Size: 12 2054 46 1208 1308
Fragments arranged by size:
2054 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 1642 2420 2610 3402 1642
Size: 778 190 792 2868
Fragments arranged by size:
2868 792 778 190
BsmI GAATG_Cn'
Cuts at: 921 921
Size: 4628
BsmBI CGTCTCn'nnnn_
Cuts at: 1469 1469
Size: 4628
Bsp1286I G_dGCh'C
Cuts at: 330 352 755 1322 1642 2253 2346 2420 2610
Size: 22 403 567 320 611 93 74 190
Cuts at: 2610 2672 3402 330
Size: 62 730 1556
Fragments arranged by size:
1556 730 611 567 403 320 190 93
74 62 22
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 1466 3804 1466
Size: 2338 2290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 435 510 548 1678 1892 2194 2575 435
Size: 75 38 1130 214 302 381 2488
Fragments arranged by size:
2488 1130 381 302 214 75 38
BsrBI CCG'CTC
Cuts at: 50 212 2920 2974 4458 4623 50
Size: 162 2708 54 1484 165 55
Fragments arranged by size:
2708 1484 165 162 55 54
BsrDI GCAATG_nn'
Cuts at: 2540 2540
Size: 4628
BsrFI r'CCGG_y
Cuts at: 244 319 2626 2807 3016 244
Size: 75 2307 181 209 1856
Fragments arranged by size:
2307 1856 209 181 75
BssHII G'CGCG_C
Cuts at: 281 281
Size: 4628
BssSI C'ACGA_G
Cuts at: 580 694 1309 2899 3257 580
Size: 114 615 1590 358 1951
Fragments arranged by size:
1951 1590 615 358 114
BstAPI GCAn_nnn'nTGC
Cuts at: 267 468 2005 267
Size: 201 1537 2890
Fragments arranged by size:
2890 1537 201
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstXI CCAn_nnnn'nTGG
Cuts at: 1107 1107
Size: 4628
BstYI r'GATC_y
Cuts at: 1 131 908 1653 2120 2143 2478 2724 3736
Size: 130 777 745 467 23 335 246 1012
Cuts at: 3736 3822 3834 4180 1
Size: 86 12 346 449
Fragments arranged by size:
1012 777 745 467 449 346 335 246
130 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 1871 1871
Size: 4628
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DraIII CAC_nnn'GTG
Cuts at: 585 1509 585
Size: 924 3704
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3192 2334
Size: 858 3770
EaeI y'GGCC_r
Cuts at: 113 803 2213 2387 2778 2805 4150 113
Size: 690 1410 174 391 27 1345 591
Fragments arranged by size:
1410 1345 690 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 2213 113
Size: 2100 2528
EarI CTCTTCn'nnn_
Cuts at: 877 970 1212 1271 2651 2861 877
Size: 93 242 59 1380 210 2644
Fragments arranged by size:
2644 1380 242 210 93 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 124 2836 3146 3292 4438 124
Size: 2712 310 146 1146 314
Fragments arranged by size:
2712 1146 314 310 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 520 617 670 937 2453 2885 3632 3900
Size: 360 97 53 267 1516 432 747 268
Cuts at: 3900 160
Size: 888
Fragments arranged by size:
1516 888 747 432 360 268 267 97
53
EcoNI CCTnn'n_nnAGG
Cuts at: 235 517 544 235
Size: 282 27 4319
Fragments arranged by size:
4319 282 27
EcoO109I rG'GnC_Cy
Cuts at: 228 326 721 1318 1767 228
Size: 98 395 597 449 3089
Fragments arranged by size:
3089 597 449 395 98
EcoRI G'AATT_C
Cuts at: 1632 1632
Size: 4628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4628
FspI TGC'GCA
Cuts at: 920 2409 920
Size: 1489 3139
HaeII r_GCGC'y
Cuts at: 1093 2062 2310 3332 4311 1093
Size: 969 248 1022 979 1410
Fragments arranged by size:
1410 1022 979 969 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 669 701 742 774 1396 1428 2470 2500 2502
Size: 32 41 32 622 32 1042 30 2
Cuts at: 2502 2532 669
Size: 30 2765
Fragments arranged by size:
2765 1042 622 41 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1490 1667 1490
Size: 177 4451
KasI G'GCGC_C
Cuts at: 1089 2058 2306 1089
Size: 969 248 3411
Fragments arranged by size:
3411 969 248
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1235 3299 3483 1235
Size: 2064 184 2380
Fragments arranged by size:
2380 2064 184
MscI TGG'CCA
Cuts at: 805 2389 805
Size: 1584 3044
MslI CAynn'nnrTG
Cuts at: 2617 2744 2617
Size: 127 4501
MspA1I CmG'CkG
Cuts at: 149 267 465 801 916 1739 2053 2413 3426
Size: 118 198 336 115 823 314 360 1013
Cuts at: 3426 3671 149
Size: 245 1106
Fragments arranged by size:
1106 1013 823 360 336 314 245 198
118 115
NaeI GCC'GGC
Cuts at: 246 321 2809 246
Size: 75 2488 2065
Fragments arranged by size:
2488 2065 75
NarI GG'CG_CC
Cuts at: 1090 2059 2307 1090
Size: 969 248 3411
Fragments arranged by size:
3411 969 248
NgoMIV G'CCGG_C
Cuts at: 244 319 2807 244
Size: 75 2488 2065
Fragments arranged by size:
2488 2065 75
NspI r_CATG'y
Cuts at: 222 1218 1238 1353 1681 2181 3088 222
Size: 996 20 115 328 500 907 1762
Fragments arranged by size:
1762 996 907 500 328 115 20
PciI A'CATG_T
Cuts at: 1214 1234 3084 1214
Size: 20 1850 2758
Fragments arranged by size:
2758 1850 20
PflMI CCAn_nnn'nTGG
Cuts at: 612 947 1314 1570 612
Size: 335 367 256 3670
Fragments arranged by size:
3670 367 335 256
PfoI T'CCnGG_A
Cuts at: 857 857
Size: 4628
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 778 810 3767 3799 778
Size: 32 2957 32 1607
Fragments arranged by size:
2957 1607 32 32
PpuMI rG'GwC_Cy
Cuts at: 721 721
Size: 4628
PshAI GACnn'nnGTC
Cuts at: 688 688
Size: 4628
PspOMI G'GGCC_C
Cuts at: 326 348 1318 326
Size: 22 970 3636
Fragments arranged by size:
3636 970 22
PstI C_TGCA'G
Cuts at: 446 464 545 1675 2360 446
Size: 18 81 1130 685 2714
Fragments arranged by size:
2714 1130 685 81 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 267 465 2053 2413 267
Size: 198 1588 360 2482
Fragments arranged by size:
2482 1588 360 198
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SalI G'TCGA_C
Cuts at: 1488 1665 1488
Size: 177 4451
SanDI GG'GwC_CC
Cuts at: 721 721
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 446 545 1675 446
Size: 99 1130 3399
Fragments arranged by size:
3399 1130 99
ScaI AGT'ACT
Cuts at: 1198 4145 1198
Size: 2947 1681
SexAI A'CCwGG_T
Cuts at: 824 824
Size: 4628
SfcI C'TryA_G
Cuts at: 33 442 460 541 1671 2356 3349 3540 33
Size: 409 18 81 1130 685 993 191 1121
Fragments arranged by size:
1130 1121 993 685 409 191 81 18
SfoI GGC'GCC
Cuts at: 1091 2060 2308 1091
Size: 969 248 3411
Fragments arranged by size:
3411 969 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SgrAI Cr'CCGG_yG
Cuts at: 319 319
Size: 4628
SmaI CCC'GGG
Cuts at: 1650 1650
Size: 4628
SmlI C'TyrA_G
Cuts at: 1399 1516 1783 2698 3190 3452 1399
Size: 117 267 915 492 262 2575
Fragments arranged by size:
2575 915 492 267 262 117
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 222 1681 2181 222
Size: 1459 500 2669
Fragments arranged by size:
2669 1459 500
StuI AGG'CCT
Cuts at: 550 550
Size: 4628
StyI C'CwwG_G
Cuts at: 1762 2116 1762
Size: 354 4274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2211 2344 2803 3012 2211
Size: 133 459 209 3827
Fragments arranged by size:
3827 459 209 133
TatI w'GTAC_w
Cuts at: 1196 1520 4143 1196
Size: 324 2623 1681
Fragments arranged by size:
2623 1681 324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4596
Tth111I GACn'n_nGTC
Cuts at: 826 2425 826
Size: 1599 3029
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1290 2123 1290
Size: 833 3795
XmaI C'CCGG_G
Cuts at: 1648 1648
Size: 4628
XmnI GAAnn'nnTTC
Cuts at: 1550 1550
Size: 4628
ZraI GAC'GTC
Cuts at: 1242 1479 1242
Size: 237 4391
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmtI BsiWI BsrGI BstEII BstZ17I
Bsu36I BtgI ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PsiI PspXI PsrI SacII SfiI
SnaBI SrfI SspI SwaI XhoI