Restriction map for
pF1KB5919
(Circular) (Six-base) MAPSORT of: pF1KB5919.seq Check: 5883 from: 1 to: 4628
pF1KB5919 4628 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 292 292
Size: 4628
AccI GT'mk_AC
Cuts at: 1103 1666 1103
Size: 563 4065
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 2453 2885 3632 3900 838
Size: 1615 432 747 268 1566
Fragments arranged by size:
1615 1566 747 432 268
AflIII A'CryG_T
Cuts at: 1980 3084 4061 1980
Size: 1104 977 2547
Fragments arranged by size:
2547 1104 977
AgeI A'CCGG_T
Cuts at: 517 3016 517
Size: 2499 2129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 137 169 137
Size: 32 4596
AlwNI CAG_nnn'CTG
Cuts at: 816 1933 3500 816
Size: 1117 1567 1944
Fragments arranged by size:
1944 1567 1117
ApaI G_GGCC'C
Cuts at: 210 210
Size: 4628
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 559 670 1333 1632 3981 4237 559
Size: 111 663 299 2349 256 950
Fragments arranged by size:
2349 950 663 299 256 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 1648 1648
Size: 4628
BamHI G'GATC_C
Cuts at: 915 1653 915
Size: 738 3890
BanI G'GyrC_C
Cuts at: 1423 1644 2058 2306 2341 1423
Size: 221 414 248 35 3710
Fragments arranged by size:
3710 414 248 221 35
BanII G_rGCy'C
Cuts at: 210 1642 2672 210
Size: 1432 1030 2166
Fragments arranged by size:
2166 1432 1030
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 1265 1577 1265
Size: 312 4316
BbvCI CC'TCA_GC
Cuts at: 281 425 281
Size: 144 4484
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3287 4628 2526
Size: 761 1341 2526
Fragments arranged by size:
2526 1341 761
BclI T'GATC_A
Cuts at: 2148 2148
Size: 4628
BglII A'GATC_T
Cuts at: 1167 2143 4180 1167
Size: 976 2037 1615
Fragments arranged by size:
2037 1615 976
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 210 1428 2253 2346 3402 210
Size: 1218 825 93 1056 1436
Fragments arranged by size:
1436 1218 1056 825 93
BmrI ACTGGGnnnn_n'
Cuts at: 216 1840 1921 2256 4400 216
Size: 1624 81 335 2144 444
Fragments arranged by size:
2144 1624 444 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 863 1162 366
Size: 497 299 3832
Fragments arranged by size:
3832 497 299
Bpu10I CC'TnA_GC
Cuts at: 281 425 281
Size: 144 4484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 1804 2683 3175 3473 309
Size: 1495 879 492 298 1464
Fragments arranged by size:
1495 1464 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4332 4332
Size: 4628
BsaAI yAC'GTr
Cuts at: 420 1981 420
Size: 1561 3067
BsaBI GATnn'nnATC
Cuts at: 6 828 2142 4179 6
Size: 822 1314 2037 455
Fragments arranged by size:
2037 1314 822 455
BsaHI Gr'CG_yC
Cuts at: 2059 2307 3009 4505 2059
Size: 248 702 1496 2182
Fragments arranged by size:
2182 1496 702 248
BsaWI w'CCGG_w
Cuts at: 517 1817 2338 3016 3290 3437 3848 517
Size: 1300 521 678 274 147 411 1297
Fragments arranged by size:
1300 1297 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 137 167 575 605 787 817 137
Size: 30 408 30 182 30 3948
Fragments arranged by size:
3948 408 182 30 30 30
BseYI C'CCAG_C
Cuts at: 717 747 2053 2811 3388 3884 717
Size: 30 1306 758 577 496 1461
Fragments arranged by size:
1461 1306 758 577 496 30
BsiEI CG_ry'CG
Cuts at: 104 2170 2216 3424 104
Size: 2066 46 1208 1308
Fragments arranged by size:
2066 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1642 2420 2610 3402 1642
Size: 778 190 792 2868
Fragments arranged by size:
2868 792 778 190
BsmI GAATG_Cn'
Cuts at: 1524 1524
Size: 4628
Bsp1286I G_dGCh'C
Cuts at: 210 1428 1642 2253 2346 2420 2610 2672 3402
Size: 1218 214 611 93 74 190 62 730
Cuts at: 3402 210
Size: 1436
Fragments arranged by size:
1436 1218 730 611 214 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 3804 3804
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 292 1678 1892 2194 2575 292
Size: 1386 214 302 381 2345
Fragments arranged by size:
2345 1386 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2920 2974 4458 4623 50
Size: 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2540 2540
Size: 4628
BsrFI r'CCGG_y
Cuts at: 517 1293 2626 2807 3016 517
Size: 776 1333 181 209 2129
Fragments arranged by size:
2129 1333 776 209 181
BssSI C'ACGA_G
Cuts at: 2899 3257 2899
Size: 358 4270
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 2005 1120
Size: 885 3743
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstXI CCAn_nnnn'nTGG
Cuts at: 360 360
Size: 4628
BstYI r'GATC_y
Cuts at: 1 737 829 915 1005 1167 1653 2120 2143
Size: 736 92 86 90 162 486 467 23
Cuts at: 2143 2478 2724 3736 3822 3834 4180 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 736 486 467 449 346 335 246
162 92 90 86 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 931 1529 931
Size: 598 4030
BtgI C'CryG_G
Cuts at: 167 451 776 167
Size: 284 325 4019
Fragments arranged by size:
4019 325 284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 893 1552 1871 893
Size: 659 319 3650
Fragments arranged by size:
3650 659 319
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 1043 2334 3192 1043
Size: 1291 858 2479
Fragments arranged by size:
2479 1291 858
EaeI y'GGCC_r
Cuts at: 237 2213 2387 2778 2805 4150 237
Size: 1976 174 391 27 1345 715
Fragments arranged by size:
1976 1345 715 391 174 27
EagI C'GGCC_G
Cuts at: 2213 2213
Size: 4628
EarI CTCTTCn'nnn_
Cuts at: 1596 2651 2861 1596
Size: 1055 210 3363
Fragments arranged by size:
3363 1055 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2836 3146 3292 4438 2836
Size: 310 146 1146 3026
Fragments arranged by size:
3026 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 838 863 1162 2453 2885 3632 3900 366
Size: 472 25 299 1291 432 747 268 1094
Fragments arranged by size:
1291 1094 747 472 432 299 268 25
EcoNI CCTnn'n_nnAGG
Cuts at: 934 934
Size: 4628
EcoO109I rG'GnC_Cy
Cuts at: 206 207 744 1767 206
Size: 1 537 1023 3067
Fragments arranged by size:
3067 1023 537 1
EcoRI G'AATT_C
Cuts at: 559 1632 559
Size: 1073 3555
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4628
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 2062 2310 3332 4311 2062
Size: 248 1022 979 2379
Fragments arranged by size:
2379 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 183 215 620 652 787 819 1060 1067 1092
Size: 32 405 32 135 32 241 7 25
Cuts at: 1092 1099 1342 1374 1431 1463 2470 2500 2502
Size: 7 243 32 57 32 1007 30 2
Cuts at: 2502 2532 183
Size: 30 2279
Fragments arranged by size:
2279 1007 405 243 241 135 57 32
32 32 32 32 30 30 25 7
7 2
HincII GTy'rAC
Cuts at: 185 1667 185
Size: 1482 3146
HindIII A'AGCT_T
Cuts at: 970 970
Size: 4628
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1487 1499 1540 3299 3483 1487
Size: 12 41 1759 184 2632
Fragments arranged by size:
2632 1759 184 41 12
MscI TGG'CCA
Cuts at: 239 2389 239
Size: 2150 2478
MslI CAynn'nnrTG
Cuts at: 2617 2744 2617
Size: 127 4501
MspA1I CmG'CkG
Cuts at: 1739 2053 2413 3426 3671 1739
Size: 314 360 1013 245 2696
Fragments arranged by size:
2696 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 4628
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 4628
NspI r_CATG'y
Cuts at: 1681 2181 3088 1681
Size: 500 907 3221
Fragments arranged by size:
3221 907 500
PciI A'CATG_T
Cuts at: 3084 3084
Size: 4628
PflMI CCAn_nnn'nTGG
Cuts at: 1443 1443
Size: 4628
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 137 169 439 471 3767 3799 137
Size: 32 270 32 3296 32 966
Fragments arranged by size:
3296 966 270 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 744 744
Size: 4628
PspOMI G'GGCC_C
Cuts at: 206 206
Size: 4628
PstI C_TGCA'G
Cuts at: 1675 2360 1675
Size: 685 3943
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 2053 2413 2053
Size: 360 4268
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SexAI A'CCwGG_T
Cuts at: 903 903
Size: 4628
SfcI C'TryA_G
Cuts at: 33 439 600 1455 1671 2356 3349 3540 33
Size: 406 161 855 216 685 993 191 1121
Fragments arranged by size:
1121 993 855 685 406 216 191 161
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 1650 1650
Size: 4628
SmlI C'TyrA_G
Cuts at: 288 1783 2698 3190 3452 288
Size: 1495 915 492 262 1464
Fragments arranged by size:
1495 1464 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
StyI C'CwwG_G
Cuts at: 1762 2116 1762
Size: 354 4274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2211 2344 2803 3012 2211
Size: 133 459 209 3827
Fragments arranged by size:
3827 459 209 133
TatI w'GTAC_w
Cuts at: 4143 4143
Size: 4628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 4145 4177 847
Size: 32 3266 32 1298
Fragments arranged by size:
3266 1298 32 32
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1273 1659 1273
Size: 386 4242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1069 2123 1069
Size: 1054 3574
XmaI C'CCGG_G
Cuts at: 1648 1648
Size: 4628
XmnI GAAnn'nnTTC
Cuts at: 654 1319 654
Size: 665 3963
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BseRI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI