Restriction map for
pF1KB5925
(Circular) (Six-base) MAPSORT of: pF1KB5925.seq Check: 5247 from: 1 to: 4625
pF1KB5925 4625 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 969 969
Size: 4625
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 738 2450 2882 3629 3897 178
Size: 560 1712 432 747 268 906
Fragments arranged by size:
1712 906 747 560 432 268
AfeI AGC'GCT
Cuts at: 1057 1057
Size: 4625
AflIII A'CryG_T
Cuts at: 1977 3081 4058 1977
Size: 1104 977 2544
Fragments arranged by size:
2544 1104 977
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AlwNI CAG_nnn'CTG
Cuts at: 828 1077 1930 3497 828
Size: 249 853 1567 1956
Fragments arranged by size:
1956 1567 853 249
ApaI G_GGCC'C
Cuts at: 428 791 915 1592 428
Size: 363 124 677 3461
Fragments arranged by size:
3461 677 363 124
ApaLI G'TGCA_C
Cuts at: 3395 3395
Size: 4625
ApoI r'AATT_y
Cuts at: 1629 3978 4234 1629
Size: 2349 256 2020
Fragments arranged by size:
2349 2020 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 428 908 1349 1645 428
Size: 480 441 296 3408
Fragments arranged by size:
3408 480 441 296
BamHI G'GATC_C
Cuts at: 1650 1650
Size: 4625
BanI G'GyrC_C
Cuts at: 1380 1641 2055 2303 2338 1380
Size: 261 414 248 35 3667
Fragments arranged by size:
3667 414 261 248 35
BanII G_rGCy'C
Cuts at: 428 791 915 1100 1262 1508 1592 1639 2669
Size: 363 124 185 162 246 84 47 1030
Cuts at: 2669 428
Size: 2384
Fragments arranged by size:
2384 1030 363 246 185 162 124 84
47
BbeI G_GCGC'C
Cuts at: 2059 2307 2059
Size: 248 4377
BbvCI CC'TCA_GC
Cuts at: 443 1179 443
Size: 736 3889
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1056 1090 1056
Size: 34 4591
BciVI GTATCCnnnnn_n'
Cuts at: 1456 2523 3284 4625 1456
Size: 1067 761 1341 1456
Fragments arranged by size:
1456 1341 1067 761
BclI T'GATC_A
Cuts at: 842 2145 842
Size: 1303 3322
BglI GCCn_nnn'nGGC
Cuts at: 355 355
Size: 4625
BglII A'GATC_T
Cuts at: 455 1307 1457 2140 4177 455
Size: 852 150 683 2037 903
Fragments arranged by size:
2037 903 852 683 150
BlpI GC'TnA_GC
Cuts at: 1737 1737
Size: 4625
Bme1580I G_kGCm'C
Cuts at: 387 428 791 915 1592 2250 2343 3399 387
Size: 41 363 124 677 658 93 1056 1613
Fragments arranged by size:
1613 1056 677 658 363 124 93 41
BmrI ACTGGGnnnn_n'
Cuts at: 118 1037 1837 1918 2253 4397 118
Size: 919 800 81 335 2144 346
Fragments arranged by size:
2144 919 800 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 486 531 783 1431 2992 486
Size: 45 252 648 1561 2119
Fragments arranged by size:
2119 1561 648 252 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 692 799 895 982 1027 1090 1182 1261
Size: 425 107 96 87 45 63 92 79
Cuts at: 1261 1426 1583 267
Size: 165 157 3309
Fragments arranged by size:
3309 425 165 157 107 96 92 87
79 63 45
Bpu10I CC'TnA_GC
Cuts at: 443 577 1179 443
Size: 134 602 3889
Fragments arranged by size:
3889 602 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1801 2680 3172 3470 1801
Size: 879 492 298 2956
Fragments arranged by size:
2956 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4329 4329
Size: 4625
BsaAI yAC'GTr
Cuts at: 1978 1978
Size: 4625
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 2056 2304 3006 4502 2056
Size: 248 702 1496 2179
Fragments arranged by size:
2179 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1814 2335 3013 3287 3434 3845 1814
Size: 521 678 274 147 411 2594
Fragments arranged by size:
2594 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 868 898 868
Size: 30 4595
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 550 719 547
Size: 3 169 4453
Fragments arranged by size:
4453 169 3
BseYI C'CCAG_C
Cuts at: 271 497 693 715 1024 1526 1605 2050 2808
Size: 226 196 22 309 502 79 445 758
Cuts at: 2808 3385 3881 271
Size: 577 496 1015
Fragments arranged by size:
1015 758 577 502 496 445 309 226
196 79 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 616 545
Size: 71 4554
BsiEI CG_ry'CG
Cuts at: 104 2167 2213 3421 104
Size: 2063 46 1208 1308
Fragments arranged by size:
2063 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1262 1508 1639 2417 2607 3399 1262
Size: 246 131 778 190 792 2488
Fragments arranged by size:
2488 792 778 246 190 131
Bsp1286I G_dGCh'C
Cuts at: 387 428 791 915 1100 1262 1508 1592 1639
Size: 41 363 124 185 162 246 84 47
Cuts at: 1639 2250 2343 2417 2607 2669 3399 387
Size: 611 93 74 190 62 730 1613
Fragments arranged by size:
1613 730 611 363 246 190 185 162
124 93 84 74 62 47 41
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 383 845 969 1675 1889 2191 2572 383
Size: 462 124 706 214 302 381 2436
Fragments arranged by size:
2436 706 462 381 302 214 124
BsrBI CCG'CTC
Cuts at: 50 1166 2917 2971 4455 4620 50
Size: 1116 1751 54 1484 165 55
Fragments arranged by size:
1751 1484 1116 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2537 2537
Size: 4625
BsrFI r'CCGG_y
Cuts at: 941 2623 2804 3013 941
Size: 1682 181 209 2553
Fragments arranged by size:
2553 1682 209 181
BsrGI T'GTAC_A
Cuts at: 1371 1371
Size: 4625
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 1536 2002 1536
Size: 466 4159
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 126 723 1005 126
Size: 597 282 3746
Fragments arranged by size:
3746 597 282
BstYI r'GATC_y
Cuts at: 1 455 1307 1457 1650 2117 2140 2475 2721
Size: 454 852 150 193 467 23 335 246
Cuts at: 2721 3733 3819 3831 4177 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 852 467 454 449 346 335 246
193 150 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 506 1031 506
Size: 525 4100
BtgI C'CryG_G
Cuts at: 686 933 1092 686
Size: 247 159 4219
Fragments arranged by size:
4219 247 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 599 1868 599
Size: 1269 3356
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 356 649 901 1581 2210 2384 2775 2802 4147
Size: 293 252 680 629 174 391 27 1345
Cuts at: 4147 356
Size: 834
Fragments arranged by size:
1345 834 680 629 391 293 252 174
27
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 539 545 952 994 2648 2858 539
Size: 6 407 42 1654 210 2306
Fragments arranged by size:
2306 1654 407 210 42 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 397 1126 2833 3143 3289 4435 397
Size: 729 1707 310 146 1146 587
Fragments arranged by size:
1707 1146 729 587 310 146
EcoICRI GAG'CTC
Cuts at: 1260 1506 1637 1260
Size: 246 131 4248
Fragments arranged by size:
4248 246 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 267 692 738 799 895 982 1027 1090
Size: 89 425 46 61 96 87 45 63
Cuts at: 1090 1182 1261 1426 1583 2450 2882 3629 3897
Size: 92 79 165 157 867 432 747 268
Cuts at: 3897 178
Size: 906
Fragments arranged by size:
906 867 747 432 425 268 165 157
96 92 89 87 79 63 61 46
45
EcoNI CCTnn'n_nnAGG
Cuts at: 1565 1565
Size: 4625
EcoO109I rG'GnC_Cy
Cuts at: 425 697 774 788 912 1122 1554 1588 1589
Size: 272 77 14 124 210 432 34 1
Cuts at: 1589 1764 425
Size: 175 3286
Fragments arranged by size:
3286 432 272 210 175 124 77 34
14 1
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1074 2644 1074
Size: 1570 3055
FspI TGC'GCA
Cuts at: 2406 2406
Size: 4625
HaeII r_GCGC'y
Cuts at: 1059 2059 2307 3329 4308 1059
Size: 1000 248 1022 979 1376
Fragments arranged by size:
1376 1022 1000 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2467 2497 2499 2529 2467
Size: 30 2 30 4563
Fragments arranged by size:
4563 30 30 2
HincII GTy'rAC
Cuts at: 216 1664 216
Size: 1448 3177
HindIII A'AGCT_T
Cuts at: 1228 1228
Size: 4625
KasI G'GCGC_C
Cuts at: 2055 2303 2055
Size: 248 4377
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3296 3480 3296
Size: 184 4441
MscI TGG'CCA
Cuts at: 1583 2386 1583
Size: 803 3822
MslI CAynn'nnrTG
Cuts at: 124 2614 2741 124
Size: 2490 127 2008
Fragments arranged by size:
2490 2008 127
MspA1I CmG'CkG
Cuts at: 188 960 1074 1094 1212 1438 1533 1609 1736
Size: 772 114 20 118 226 95 76 127
Cuts at: 1736 2050 2410 3423 3668 188
Size: 314 360 1013 245 1145
Fragments arranged by size:
1145 1013 772 360 314 245 226 127
118 114 95 76 20
NaeI GCC'GGC
Cuts at: 943 2806 943
Size: 1863 2762
NarI GG'CG_CC
Cuts at: 2056 2304 2056
Size: 248 4377
NcoI C'CATG_G
Cuts at: 933 933
Size: 4625
NgoMIV G'CCGG_C
Cuts at: 941 2804 941
Size: 1863 2762
NspI r_CATG'y
Cuts at: 256 438 858 1617 1678 2178 3085 256
Size: 182 420 759 61 500 907 1796
Fragments arranged by size:
1796 907 759 500 420 182 61
PasI CC'CwG_GG
Cuts at: 199 199
Size: 4625
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PflMI CCAn_nnn'nTGG
Cuts at: 1077 1077
Size: 4625
PfoI T'CCnGG_A
Cuts at: 776 1085 1560 776
Size: 309 475 3841
Fragments arranged by size:
3841 475 309
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 697 774 1122 697
Size: 77 348 4200
Fragments arranged by size:
4200 348 77
PspOMI G'GGCC_C
Cuts at: 424 787 911 1588 424
Size: 363 124 677 3461
Fragments arranged by size:
3461 677 363 124
PstI C_TGCA'G
Cuts at: 560 665 680 816 1672 2357 560
Size: 105 15 136 856 685 2828
Fragments arranged by size:
2828 856 685 136 105 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 188 960 1074 1438 1609 2050 2410 188
Size: 772 114 364 171 441 360 2403
Fragments arranged by size:
2403 772 441 364 360 171 114
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 1262 1508 1639 1262
Size: 246 131 4248
Fragments arranged by size:
4248 246 131
SacII CC_GC'GG
Cuts at: 1095 1095
Size: 4625
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SanDI GG'GwC_CC
Cuts at: 697 697
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 545 952 2648 2858 545
Size: 407 1696 210 2312
Fragments arranged by size:
2312 1696 407 210
SbfI CC_TGCA'GG
Cuts at: 816 1672 816
Size: 856 3769
ScaI AGT'ACT
Cuts at: 4142 4142
Size: 4625
SfcI C'TryA_G
Cuts at: 33 556 661 676 812 951 1111 1385 1453
Size: 523 105 15 136 139 160 274 68
Cuts at: 1453 1668 2353 3346 3537 33
Size: 215 685 993 191 1121
Fragments arranged by size:
1121 993 685 523 274 215 191 160
139 136 105 68 15
SfoI GGC'GCC
Cuts at: 2057 2305 2057
Size: 248 4377
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 430 910 1647 430
Size: 480 737 3408
Fragments arranged by size:
3408 737 480
SmlI C'TyrA_G
Cuts at: 1780 2695 3187 3449 1780
Size: 915 492 262 2956
Fragments arranged by size:
2956 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 256 1617 1678 2178 256
Size: 1361 61 500 2703
Fragments arranged by size:
2703 1361 500 61
StuI AGG'CCT
Cuts at: 304 1571 304
Size: 1267 3358
StyI C'CwwG_G
Cuts at: 311 933 1572 1759 2113 311
Size: 622 639 187 354 2823
Fragments arranged by size:
2823 639 622 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 883 985 1619 2208 2341 2800 3009 883
Size: 102 634 589 133 459 209 2499
Fragments arranged by size:
2499 634 589 459 209 133 102
TatI w'GTAC_w
Cuts at: 1371 4140 1371
Size: 2769 1856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 631 663 4142 4174 631
Size: 32 3479 32 1082
Fragments arranged by size:
3479 1082 32 32
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 206 1558 2120 206
Size: 1352 562 2711
Fragments arranged by size:
2711 1352 562
XmaI C'CCGG_G
Cuts at: 428 908 1645 428
Size: 480 737 3408
Fragments arranged by size:
3408 737 480
XmnI GAAnn'nnTTC
Cuts at: 232 232
Size: 4625
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI