Restriction map for
pF1KB5926
(Circular) (Six-base) MAPSORT of: pF1KB5926.seq Check: 698 from: 1 to: 4721
pF1KB5926 4721 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1479 1479
Size: 4721
AccI GT'mk_AC
Cuts at: 1759 1759
Size: 4721
Acc65I G'GTAC_C
Cuts at: 1737 1737
Size: 4721
AclI AA'CG_TT
Cuts at: 4561 4561
Size: 4721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 1012 1708 2546 2978 3725 3993 686
Size: 326 696 838 432 747 268 1414
Fragments arranged by size:
1414 838 747 696 432 326 268
AflIII A'CryG_T
Cuts at: 2073 3177 4154 2073
Size: 1104 977 2640
Fragments arranged by size:
2640 1104 977
AgeI A'CCGG_T
Cuts at: 3109 3109
Size: 4721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1165 1165
Size: 4721
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 388 420 670 702 388
Size: 32 250 32 4407
Fragments arranged by size:
4407 250 32 32
AlwNI CAG_nnn'CTG
Cuts at: 204 664 987 990 1348 1635 2026 3593 204
Size: 460 323 3 358 287 391 1567 1332
Fragments arranged by size:
1567 1332 460 391 358 323 287 3
ApaI G_GGCC'C
Cuts at: 1714 1714
Size: 4721
ApaLI G'TGCA_C
Cuts at: 3491 3491
Size: 4721
ApoI r'AATT_y
Cuts at: 1725 4074 4330 1725
Size: 2349 256 2116
Fragments arranged by size:
2349 2116 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 298 1741 298
Size: 1443 3278
BamHI G'GATC_C
Cuts at: 1220 1746 1220
Size: 526 4195
BanI G'GyrC_C
Cuts at: 156 1737 2151 2399 2434 156
Size: 1581 414 248 35 2443
Fragments arranged by size:
2443 1581 414 248 35
BanII G_rGCy'C
Cuts at: 131 278 449 1714 1735 2765 131
Size: 147 171 1265 21 1030 2087
Fragments arranged by size:
2087 1265 1030 171 147 21
BbeI G_GCGC'C
Cuts at: 160 2155 2403 160
Size: 1995 248 2478
Fragments arranged by size:
2478 1995 248
BbsI GAAGACnn'nnnn_
Cuts at: 1000 1000
Size: 4721
BbvCI CC'TCA_GC
Cuts at: 140 1109 1177 1239 140
Size: 969 68 62 3622
Fragments arranged by size:
3622 969 68 62
BciVI GTATCCnnnnn_n'
Cuts at: 2619 3380 4721 2619
Size: 761 1341 2619
Fragments arranged by size:
2619 1341 761
BclI T'GATC_A
Cuts at: 2241 2241
Size: 4721
BglI GCCn_nnn'nGGC
Cuts at: 629 629
Size: 4721
BglII A'GATC_T
Cuts at: 1427 2236 4273 1427
Size: 809 2037 1875
Fragments arranged by size:
2037 1875 809
BlpI GC'TnA_GC
Cuts at: 1833 1833
Size: 4721
Bme1580I G_kGCm'C
Cuts at: 513 1095 1714 2346 2439 3495 513
Size: 582 619 632 93 1056 1739
Fragments arranged by size:
1739 1056 632 619 582 93
BmrI ACTGGGnnnn_n'
Cuts at: 294 1030 1933 2014 2349 4493 294
Size: 736 903 81 335 2144 522
Fragments arranged by size:
2144 903 736 522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 4721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 370 641 1312 1414 193
Size: 177 271 671 102 3500
Fragments arranged by size:
3500 671 271 177 102
Bpu10I CC'TnA_GC
Cuts at: 140 1109 1177 1239 140
Size: 969 68 62 3622
Fragments arranged by size:
3622 969 68 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1897 2776 3268 3566 1897
Size: 879 492 298 3052
Fragments arranged by size:
3052 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 798 1132 1618 4425 798
Size: 334 486 2807 1094
Fragments arranged by size:
2807 1094 486 334
BsaAI yAC'GTr
Cuts at: 2074 2074
Size: 4721
BsaBI GATnn'nnATC
Cuts at: 6 2235 4272 6
Size: 2229 2037 455
Fragments arranged by size:
2229 2037 455
BsaHI Gr'CG_yC
Cuts at: 157 2152 2400 3102 4598 157
Size: 1995 248 702 1496 280
Fragments arranged by size:
1995 1496 702 280 248
BsaWI w'CCGG_w
Cuts at: 117 1910 2431 3109 3383 3530 3941 117
Size: 1793 521 678 274 147 411 897
Fragments arranged by size:
1793 897 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 670 700 994 1024 670
Size: 30 294 30 4367
Fragments arranged by size:
4367 294 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 439 688 1018 331
Size: 108 249 330 4034
Fragments arranged by size:
4034 330 249 108
BseYI C'CCAG_C
Cuts at: 255 269 470 618 689 1069 1094 1419 1635
Size: 14 201 148 71 380 25 325 216
Cuts at: 1635 2146 2904 3481 3977 255
Size: 511 758 577 496 999
Fragments arranged by size:
999 758 577 511 496 380 325 216
201 148 71 25 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1125 349
Size: 776 3945
BsiEI CG_ry'CG
Cuts at: 104 326 345 2263 2309 3517 104
Size: 222 19 1918 46 1208 1308
Fragments arranged by size:
1918 1308 1208 222 46 19
BsiHKAI G_wGCw'C
Cuts at: 248 1328 1735 2513 2703 3495 248
Size: 1080 407 778 190 792 1474
Fragments arranged by size:
1474 1080 792 778 407 190
Bsp1286I G_dGCh'C
Cuts at: 131 248 278 449 513 1095 1328 1714 1735
Size: 117 30 171 64 582 233 386 21
Cuts at: 1735 2346 2439 2513 2703 2765 3495 131
Size: 611 93 74 190 62 730 1357
Fragments arranged by size:
1357 730 611 582 386 233 190 171
117 93 74 64 62 30 21
BspEI T'CCGG_A
Cuts at: 3941 3941
Size: 4721
BspHI T'CATG_A
Cuts at: 792 3897 792
Size: 3105 1616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1479 1771 1985 2287 2668 1479
Size: 292 214 302 381 3532
Fragments arranged by size:
3532 381 302 292 214
BsrBI CCG'CTC
Cuts at: 50 1485 3013 3067 4551 4716 50
Size: 1435 1528 54 1484 165 55
Fragments arranged by size:
1528 1484 1435 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1188 2633 1188
Size: 1445 3276
BsrFI r'CCGG_y
Cuts at: 153 321 2719 2900 3109 153
Size: 168 2398 181 209 1765
Fragments arranged by size:
2398 1765 209 181 168
BssSI C'ACGA_G
Cuts at: 2992 3350 2992
Size: 358 4363
BstAPI GCAn_nnn'nTGC
Cuts at: 204 987 2098 204
Size: 783 1111 2827
Fragments arranged by size:
2827 1111 783
BstBI TT'CG_AA
Cuts at: 904 3082 904
Size: 2178 2543
BstXI CCAn_nnnn'nTGG
Cuts at: 697 1077 1566 697
Size: 380 489 3852
Fragments arranged by size:
3852 489 380
BstYI r'GATC_y
Cuts at: 1 1220 1427 1746 2213 2236 2571 2817 3829
Size: 1219 207 319 467 23 335 246 1012
Cuts at: 3829 3915 3927 4273 1
Size: 86 12 346 449
Fragments arranged by size:
1219 1012 467 449 346 335 319 246
207 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 218 740 1675 218
Size: 522 935 3264
Fragments arranged by size:
3264 935 522
BtgI C'CryG_G
Cuts at: 107 264 554 1565 107
Size: 157 290 1011 3263
Fragments arranged by size:
3263 1011 290 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 413 2671 2852 3015 413
Size: 2258 181 163 2119
Fragments arranged by size:
2258 2119 181 163
BtsI GCAGTG_nn'
Cuts at: 1964 1964
Size: 4721
DraI TTT'AAA
Cuts at: 1720 1720
Size: 4721
DrdI GACnn_nn'nnGTC
Cuts at: 871 2427 3285 871
Size: 1556 858 2307
Fragments arranged by size:
2307 1556 858
EaeI y'GGCC_r
Cuts at: 342 416 424 2306 2480 2871 2898 4243 342
Size: 74 8 1882 174 391 27 1345 820
Fragments arranged by size:
1882 1345 820 391 174 74 27 8
EagI C'GGCC_G
Cuts at: 342 2306 342
Size: 1964 2757
EarI CTCTTCn'nnn_
Cuts at: 665 719 742 773 2744 2954 665
Size: 54 23 31 1971 210 2432
Fragments arranged by size:
2432 1971 210 54 31 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2929 3239 3385 4531 2929
Size: 310 146 1146 3119
Fragments arranged by size:
3119 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1733 1733
Size: 4721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 370 641 686 1012 1312 1414 1708 2546
Size: 177 271 45 326 300 102 294 838
Cuts at: 2546 2978 3725 3993 193
Size: 432 747 268 921
Fragments arranged by size:
921 838 747 432 326 300 294 271
268 177 102 45
EcoNI CCTnn'n_nnAGG
Cuts at: 196 1674 196
Size: 1478 3243
EcoO109I rG'GnC_Cy
Cuts at: 177 214 303 334 477 548 753 1332 1345
Size: 37 89 31 143 71 205 579 13
Cuts at: 1345 1711 1860 177
Size: 366 149 3038
Fragments arranged by size:
3038 579 366 205 149 143 89 71
37 31 13
EcoRI G'AATT_C
Cuts at: 1725 1725
Size: 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1713 2740 1713
Size: 1027 3694
FspI TGC'GCA
Cuts at: 2502 2502
Size: 4721
HaeII r_GCGC'y
Cuts at: 160 205 256 1310 2155 2403 3425 4404 160
Size: 45 51 1054 845 248 1022 979 477
Fragments arranged by size:
1054 1022 979 845 477 248 51 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1208 1240 1590 1622 2563 2593 2595 2625 1208
Size: 32 350 32 941 30 2 30 3304
Fragments arranged by size:
3304 941 350 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1760 1760
Size: 4721
KasI G'GCGC_C
Cuts at: 156 2151 2399 156
Size: 1995 248 2478
Fragments arranged by size:
2478 1995 248
KpnI G_GTAC'C
Cuts at: 1741 1741
Size: 4721
MluI A'CGCG_T
Cuts at: 4154 4154
Size: 4721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3392 3576 3392
Size: 184 4537
MscI TGG'CCA
Cuts at: 2482 2482
Size: 4721
MslI CAynn'nnrTG
Cuts at: 1564 2710 2837 1564
Size: 1146 127 3448
Fragments arranged by size:
3448 1146 127
MspA1I CmG'CkG
Cuts at: 171 266 987 1098 1832 2146 2506 3519 3764
Size: 95 721 111 734 314 360 1013 245
Cuts at: 3764 171
Size: 1128
Fragments arranged by size:
1128 1013 734 721 360 314 245 111
95
NaeI GCC'GGC
Cuts at: 155 2902 155
Size: 2747 1974
NarI GG'CG_CC
Cuts at: 157 2152 2400 157
Size: 1995 248 2478
Fragments arranged by size:
2478 1995 248
NcoI C'CATG_G
Cuts at: 107 554 1565 107
Size: 447 1011 3263
Fragments arranged by size:
3263 1011 447
NgoMIV G'CCGG_C
Cuts at: 153 2900 153
Size: 2747 1974
NspI r_CATG'y
Cuts at: 1774 2274 3181 1774
Size: 500 907 3314
Fragments arranged by size:
3314 907 500
PasI CC'CwG_GG
Cuts at: 133 161 513 133
Size: 28 352 4341
Fragments arranged by size:
4341 352 28
PciI A'CATG_T
Cuts at: 3177 3177
Size: 4721
PflMI CCAn_nnn'nTGG
Cuts at: 1635 1635
Size: 4721
PfoI T'CCnGG_A
Cuts at: 959 959
Size: 4721
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 670 702 3860 3892 670
Size: 32 3158 32 1499
Fragments arranged by size:
3158 1499 32 32
PpuMI rG'GwC_Cy
Cuts at: 477 548 753 1345 477
Size: 71 205 592 3853
Fragments arranged by size:
3853 592 205 71
PshAI GACnn'nnGTC
Cuts at: 287 344 1124 287
Size: 57 780 3884
Fragments arranged by size:
3884 780 57
PspOMI G'GGCC_C
Cuts at: 1710 1710
Size: 4721
PspXI vC'TCGA_Gb
Cuts at: 298 298
Size: 4721
PstI C_TGCA'G
Cuts at: 200 209 464 983 1103 1306 1768 2453 200
Size: 9 255 519 120 203 462 685 2468
Fragments arranged by size:
2468 685 519 462 255 203 120 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 171 987 1098 2146 2506 171
Size: 816 111 1048 360 2386
Fragments arranged by size:
2386 1048 816 360 111
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 4721
SacI G_AGCT'C
Cuts at: 1735 1735
Size: 4721
SacII CC_GC'GG
Cuts at: 267 267
Size: 4721
SalI G'TCGA_C
Cuts at: 1758 1758
Size: 4721
SanDI GG'GwC_CC
Cuts at: 753 753
Size: 4721
SapI GCTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
SbfI CC_TGCA'GG
Cuts at: 1768 1768
Size: 4721
ScaI AGT'ACT
Cuts at: 4238 4238
Size: 4721
SfcI C'TryA_G
Cuts at: 33 196 205 460 979 1045 1099 1302 1764
Size: 163 9 255 519 66 54 203 462
Cuts at: 1764 2449 3442 3633 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 519 462 255 203 191
163 66 54 9
SfoI GGC'GCC
Cuts at: 158 2153 2401 158
Size: 1995 248 2478
Fragments arranged by size:
2478 1995 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 1743 1743
Size: 4721
SmlI C'TyrA_G
Cuts at: 298 1876 2791 3283 3545 298
Size: 1578 915 492 262 1474
Fragments arranged by size:
1578 1474 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4721
SphI G_CATG'C
Cuts at: 1774 2274 1774
Size: 500 4221
StyI C'CwwG_G
Cuts at: 107 554 998 1565 1855 2209 107
Size: 447 444 567 290 354 2619
Fragments arranged by size:
2619 567 447 444 354 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1114 2304 2437 2896 3105 1114
Size: 1190 133 459 209 2730
Fragments arranged by size:
2730 1190 459 209 133
TatI w'GTAC_w
Cuts at: 4236 4236
Size: 4721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1072 1104 4238 4270 1072
Size: 32 3134 32 1523
Fragments arranged by size:
3134 1523 32 32
Tth111I GACn'n_nGTC
Cuts at: 2518 2518
Size: 4721
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1563 2216 1563
Size: 653 4068
XhoI C'TCGA_G
Cuts at: 298 298
Size: 4721
XmaI C'CCGG_G
Cuts at: 1741 1741
Size: 4721
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PsiI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI