Restriction map for
pF1KB5927
(Circular) (Six-base) MAPSORT of: pF1KB5927.seq Check: 4777 from: 1 to: 4814
pF1KB5927 4814 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 420 420
Size: 4814
AccI GT'mk_AC
Cuts at: 406 1517 1852 406
Size: 1111 335 3368
Fragments arranged by size:
3368 1111 335
Acc65I G'GTAC_C
Cuts at: 1830 1830
Size: 4814
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 2639 3071 3818 4086 796
Size: 1843 432 747 268 1524
Fragments arranged by size:
1843 1524 747 432 268
AflIII A'CryG_T
Cuts at: 2166 3270 4247 2166
Size: 1104 977 2733
Fragments arranged by size:
2733 1104 977
AgeI A'CCGG_T
Cuts at: 3202 3202
Size: 4814
AhdI GACnn_n'nnGTC
Cuts at: 1798 1798
Size: 4814
AleI CACnn'nnGTG
Cuts at: 292 292
Size: 4814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 244 244
Size: 4814
AlwNI CAG_nnn'CTG
Cuts at: 476 2119 3686 476
Size: 1643 1567 1604
Fragments arranged by size:
1643 1604 1567
ApaI G_GGCC'C
Cuts at: 852 852
Size: 4814
ApaLI G'TGCA_C
Cuts at: 3584 3584
Size: 4814
ApoI r'AATT_y
Cuts at: 643 661 1378 1818 4167 4423 643
Size: 18 717 440 2349 256 1034
Fragments arranged by size:
2349 1034 717 440 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 1834 1834
Size: 4814
AvrII C'CTAG_G
Cuts at: 1011 1011
Size: 4814
BamHI G'GATC_C
Cuts at: 1422 1692 1839 1422
Size: 270 147 4397
Fragments arranged by size:
4397 270 147
BanI G'GyrC_C
Cuts at: 1830 2244 2492 2527 1830
Size: 414 248 35 4117
Fragments arranged by size:
4117 414 248 35
BanII G_rGCy'C
Cuts at: 121 852 1039 1735 1828 2858 121
Size: 731 187 696 93 1030 2077
Fragments arranged by size:
2077 1030 731 696 187 93
BbeI G_GCGC'C
Cuts at: 2248 2496 2248
Size: 248 4566
BbsI GAAGACnn'nnnn_
Cuts at: 642 893 1537 642
Size: 251 644 3919
Fragments arranged by size:
3919 644 251
BciVI GTATCCnnnnn_n'
Cuts at: 2712 3473 4814 2712
Size: 761 1341 2712
Fragments arranged by size:
2712 1341 761
BclI T'GATC_A
Cuts at: 2334 2334
Size: 4814
BfrBI ATG'CAT
Cuts at: 1612 1612
Size: 4814
BglI GCCn_nnn'nGGC
Cuts at: 1022 1022
Size: 4814
BglII A'GATC_T
Cuts at: 2329 4366 2329
Size: 2037 2777
BlpI GC'TnA_GC
Cuts at: 1729 1926 1729
Size: 197 4617
Bme1580I G_kGCm'C
Cuts at: 143 852 2439 2532 3588 143
Size: 709 1587 93 1056 1369
Fragments arranged by size:
1587 1369 1056 709 93
BmrI ACTGGGnnnn_n'
Cuts at: 688 1380 2026 2107 2442 4586 688
Size: 692 646 81 335 2144 916
Fragments arranged by size:
2144 916 692 646 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 4814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 1477 1626 503
Size: 974 149 3691
Fragments arranged by size:
3691 974 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1990 2869 3361 3659 1990
Size: 879 492 298 3145
Fragments arranged by size:
3145 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 423 1177 4518 423
Size: 754 3341 719
Fragments arranged by size:
3341 754 719
BsaAI yAC'GTr
Cuts at: 1551 2167 1551
Size: 616 4198
BsaBI GATnn'nnATC
Cuts at: 6 704 2328 4365 6
Size: 698 1624 2037 455
Fragments arranged by size:
2037 1624 698 455
BsaHI Gr'CG_yC
Cuts at: 2245 2493 3195 4691 2245
Size: 248 702 1496 2368
Fragments arranged by size:
2368 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2003 2524 3202 3476 3623 4034 2003
Size: 521 678 274 147 411 2783
Fragments arranged by size:
2783 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 971 1134 971
Size: 163 4651
BseYI C'CCAG_C
Cuts at: 766 843 852 1188 1696 2239 2997 3574 4070
Size: 77 9 336 508 543 758 577 496
Cuts at: 4070 766
Size: 1510
Fragments arranged by size:
1510 758 577 543 508 496 336 77
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 367 521 270
Size: 97 154 4563
Fragments arranged by size:
4563 154 97
BsiEI CG_ry'CG
Cuts at: 104 169 173 276 2356 2402 3610 104
Size: 65 4 103 2080 46 1208 1308
Fragments arranged by size:
2080 1308 1208 103 65 46 4
BsiHKAI G_wGCw'C
Cuts at: 1828 2606 2796 3588 1828
Size: 778 190 792 3054
Fragments arranged by size:
3054 792 778 190
BsmI GAATG_Cn'
Cuts at: 1079 1079
Size: 4814
Bsp1286I G_dGCh'C
Cuts at: 121 143 852 1039 1735 1828 2439 2532 2606
Size: 22 709 187 696 93 611 93 74
Cuts at: 2606 2796 2858 3588 121
Size: 190 62 730 1347
Fragments arranged by size:
1347 730 709 696 611 190 187 93
93 74 62 22
BspEI T'CCGG_A
Cuts at: 4034 4034
Size: 4814
BspHI T'CATG_A
Cuts at: 1445 1770 3990 1445
Size: 325 2220 2269
Fragments arranged by size:
2269 2220 325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 275 302 420 1391 1864 2078 2380 2761
Size: 39 27 118 971 473 214 302 381
Cuts at: 2761 236
Size: 2289
Fragments arranged by size:
2289 971 473 381 302 214 118 39
27
BsrBI CCG'CTC
Cuts at: 50 3106 3160 4644 4809 50
Size: 3056 54 1484 165 55
Fragments arranged by size:
3056 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 800 953 1058 2726 800
Size: 153 105 1668 2888
Fragments arranged by size:
2888 1668 153 105
BsrFI r'CCGG_y
Cuts at: 168 172 199 2812 2993 3202 168
Size: 4 27 2613 181 209 1780
Fragments arranged by size:
2613 1780 209 181 27 4
BssSI C'ACGA_G
Cuts at: 3085 3443 3085
Size: 358 4456
BstAPI GCAn_nnn'nTGC
Cuts at: 985 2191 985
Size: 1206 3608
BstBI TT'CG_AA
Cuts at: 3175 3175
Size: 4814
BstXI CCAn_nnnn'nTGG
Cuts at: 297 481 1196 297
Size: 184 715 3915
Fragments arranged by size:
3915 715 184
BstYI r'GATC_y
Cuts at: 1 1422 1692 1839 2306 2329 2664 2910 3922
Size: 1421 270 147 467 23 335 246 1012
Cuts at: 3922 4008 4020 4366 1
Size: 86 12 346 449
Fragments arranged by size:
1421 1012 467 449 346 335 270 246
147 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1591 1591
Size: 4814
BtgI C'CryG_G
Cuts at: 107 290 356 1107 107
Size: 183 66 751 3814
Fragments arranged by size:
3814 751 183 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1731 2764 2945 3108 1731
Size: 1033 181 163 3437
Fragments arranged by size:
3437 1033 181 163
BtsI GCAGTG_nn'
Cuts at: 474 1730 2057 474
Size: 1256 327 3231
Fragments arranged by size:
3231 1256 327
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1224 1259 1378 1413 1224
Size: 35 119 35 4625
Fragments arranged by size:
4625 119 35 35
DraI TTT'AAA
Cuts at: 1813 1813
Size: 4814
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3378 2520
Size: 858 3956
EaeI y'GGCC_r
Cuts at: 166 170 329 353 359 1052 1154 1383 1488
Size: 4 159 24 6 693 102 229 105
Cuts at: 1488 1701 1782 2399 2573 2964 2991 4336 166
Size: 213 81 617 174 391 27 1345 644
Fragments arranged by size:
1345 693 644 617 391 229 213 174
159 105 102 81 27 24 6 4
EagI C'GGCC_G
Cuts at: 166 170 2399 166
Size: 4 2229 2581
Fragments arranged by size:
2581 2229 4
EarI CTCTTCn'nnn_
Cuts at: 211 941 1244 2837 3047 211
Size: 730 303 1593 210 1978
Fragments arranged by size:
1978 1593 730 303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3022 3332 3478 4624 3022
Size: 310 146 1146 3212
Fragments arranged by size:
3212 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1826 1826
Size: 4814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 796 1477 1626 2639 3071 3818 4086 503
Size: 293 681 149 1013 432 747 268 1231
Fragments arranged by size:
1231 1013 747 681 432 293 268 149
EcoNI CCTnn'n_nnAGG
Cuts at: 208 208
Size: 4814
EcoO109I rG'GnC_Cy
Cuts at: 532 811 848 849 1170 1452 1461 1587 1792
Size: 279 37 1 321 282 9 126 205
Cuts at: 1792 1953 532
Size: 161 3393
Fragments arranged by size:
3393 321 282 279 205 161 126 37
9 1
EcoRI G'AATT_C
Cuts at: 1818 1818
Size: 4814
EcoRV GAT'ATC
Cuts at: 702 702
Size: 4814
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4814
FseI GG_CCGG'CC
Cuts at: 172 172
Size: 4814
FspI TGC'GCA
Cuts at: 2595 2595
Size: 4814
HaeII r_GCGC'y
Cuts at: 2248 2496 3518 4497 2248
Size: 248 1022 979 2565
Fragments arranged by size:
2565 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 535 567 1784 1816 2656 2686 2688 2718 535
Size: 32 1217 32 840 30 2 30 2631
Fragments arranged by size:
2631 1217 840 32 32 30 30 2
HincII GTy'rAC
Cuts at: 407 1853 407
Size: 1446 3368
KasI G'GCGC_C
Cuts at: 2244 2492 2244
Size: 248 4566
KpnI G_GTAC'C
Cuts at: 1834 1834
Size: 4814
MluI A'CGCG_T
Cuts at: 4247 4247
Size: 4814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1400 3485 3669 1400
Size: 2085 184 2545
Fragments arranged by size:
2545 2085 184
MscI TGG'CCA
Cuts at: 1054 1156 1490 2575 1054
Size: 102 334 1085 3293
Fragments arranged by size:
3293 1085 334 102
MslI CAynn'nnrTG
Cuts at: 127 292 295 2803 2930 127
Size: 165 3 2508 127 2011
Fragments arranged by size:
2508 2011 165 127 3
MspA1I CmG'CkG
Cuts at: 165 358 448 1109 1192 1700 1925 2239 2599
Size: 193 90 661 83 508 225 314 360
Cuts at: 2599 3612 3857 165
Size: 1013 245 1122
Fragments arranged by size:
1122 1013 661 508 360 314 245 225
193 90 83
NaeI GCC'GGC
Cuts at: 170 174 2995 170
Size: 4 2821 1989
Fragments arranged by size:
2821 1989 4
NarI GG'CG_CC
Cuts at: 2245 2493 2245
Size: 248 4566
NcoI C'CATG_G
Cuts at: 107 290 107
Size: 183 4631
NdeI CA'TA_TG
Cuts at: 924 924
Size: 4814
NgoMIV G'CCGG_C
Cuts at: 168 172 2993 168
Size: 4 2821 1989
Fragments arranged by size:
2821 1989 4
NsiI A_TGCA'T
Cuts at: 1614 1614
Size: 4814
NspI r_CATG'y
Cuts at: 1867 2367 3274 1867
Size: 500 907 3407
Fragments arranged by size:
3407 907 500
PciI A'CATG_T
Cuts at: 3270 3270
Size: 4814
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 4782
PpuMI rG'GwC_Cy
Cuts at: 532 811 1170 1452 1792 532
Size: 279 359 282 340 3554
Fragments arranged by size:
3554 359 340 282 279
PspOMI G'GGCC_C
Cuts at: 848 848
Size: 4814
PstI C_TGCA'G
Cuts at: 1861 2546 1861
Size: 685 4129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4814
PvuII CAG'CTG
Cuts at: 448 1192 2239 2599 448
Size: 744 1047 360 2663
Fragments arranged by size:
2663 1047 744 360
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 1828 1828
Size: 4814
SacII CC_GC'GG
Cuts at: 359 1110 359
Size: 751 4063
SalI G'TCGA_C
Cuts at: 405 1851 405
Size: 1446 3368
SanDI GG'GwC_CC
Cuts at: 1792 1792
Size: 4814
SapI GCTCTTCn'nnn_
Cuts at: 2837 3047 2837
Size: 210 4604
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4814
ScaI AGT'ACT
Cuts at: 4331 4331
Size: 4814
SfcI C'TryA_G
Cuts at: 33 1518 1857 2542 3535 3726 33
Size: 1485 339 685 993 191 1121
Fragments arranged by size:
1485 1121 993 685 339 191
SfoI GGC'GCC
Cuts at: 2246 2494 2246
Size: 248 4566
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SmaI CCC'GGG
Cuts at: 1836 1836
Size: 4814
SmlI C'TyrA_G
Cuts at: 1969 2884 3376 3638 1969
Size: 915 492 262 3145
Fragments arranged by size:
3145 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4814
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4314
StyI C'CwwG_G
Cuts at: 107 290 1011 1948 2302 107
Size: 183 721 937 354 2619
Fragments arranged by size:
2619 937 721 354 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2397 2530 2989 3198 2397
Size: 133 459 209 4013
Fragments arranged by size:
4013 459 209 133
TatI w'GTAC_w
Cuts at: 4329 4329
Size: 4814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 803 835 1282 1314 1329 1361 4331 4363 803
Size: 32 447 32 15 32 2970 32 1254
Fragments arranged by size:
2970 1254 447 32 32 32 32 15
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4814
XbaI T'CTAG_A
Cuts at: 1845 1845
Size: 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 339 1140 2309 339
Size: 801 1169 2844
Fragments arranged by size:
2844 1169 801
XmaI C'CCGG_G
Cuts at: 1834 1834
Size: 4814
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI BaeI BbvCI BcgI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI DraIII FspAI HindIII HpaI MfeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI