Restriction map for
pF1KB5930
(Circular) (Six-base) MAPSORT of: pF1KB5930.seq Check: 4207 from: 1 to: 4727
pF1KB5930 4727 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 355 693 827 355
Size: 338 134 4255
Fragments arranged by size:
4255 338 134
AccI GT'mk_AC
Cuts at: 1765 1765
Size: 4727
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 553 577 892 2552 2984 3731 3999 301
Size: 252 24 315 1660 432 747 268 1029
Fragments arranged by size:
1660 1029 747 432 315 268 252 24
AflIII A'CryG_T
Cuts at: 1571 2079 3183 4160 1571
Size: 508 1104 977 2138
Fragments arranged by size:
2138 1104 977 508
AgeI A'CCGG_T
Cuts at: 3115 3115
Size: 4727
AlwNI CAG_nnn'CTG
Cuts at: 1160 1246 2032 3599 1160
Size: 86 786 1567 2288
Fragments arranged by size:
2288 1567 786 86
ApaI G_GGCC'C
Cuts at: 719 1430 719
Size: 711 4016
ApaLI G'TGCA_C
Cuts at: 986 3497 986
Size: 2511 2216
ApoI r'AATT_y
Cuts at: 1731 4080 4336 1731
Size: 2349 256 2122
Fragments arranged by size:
2349 2122 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 687 1179 1636 1747 687
Size: 492 457 111 3667
Fragments arranged by size:
3667 492 457 111
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 1035 1743 2157 2405 2440 1035
Size: 708 414 248 35 3322
Fragments arranged by size:
3322 708 414 248 35
BanII G_rGCy'C
Cuts at: 719 1030 1081 1189 1337 1430 1546 1597 1705
Size: 311 51 108 148 93 116 51 108
Cuts at: 1705 1741 2771 719
Size: 36 1030 2675
Fragments arranged by size:
2675 1030 311 148 116 108 108 93
51 51 36
BbeI G_GCGC'C
Cuts at: 2161 2409 2161
Size: 248 4479
BbsI GAAGACnn'nnnn_
Cuts at: 542 840 880 542
Size: 298 40 4389
Fragments arranged by size:
4389 298 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 523 557 90
Size: 34 399 34 4260
Fragments arranged by size:
4260 399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2625 3386 4727 2625
Size: 761 1341 2625
Fragments arranged by size:
2625 1341 761
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglII A'GATC_T
Cuts at: 2242 4279 2242
Size: 2037 2690
BlpI GC'TnA_GC
Cuts at: 1188 1839 1188
Size: 651 4076
Bme1580I G_kGCm'C
Cuts at: 719 990 1040 1148 1430 2352 2445 3501 719
Size: 271 50 108 282 922 93 1056 1945
Fragments arranged by size:
1945 1056 922 282 271 108 93 50
BmrI ACTGGGnnnn_n'
Cuts at: 777 1491 1939 2020 2355 4499 777
Size: 714 448 81 335 2144 1005
Fragments arranged by size:
2144 1005 714 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 312 1056 1560 3094 312
Size: 744 504 1534 1945
Fragments arranged by size:
1945 1534 744 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 181 691 768 885 987 1030 1044 1069
Size: 48 510 77 117 102 43 14 25
Cuts at: 1069 1351 1538 133
Size: 282 187 3322
Fragments arranged by size:
3322 510 282 187 117 102 77 48
43 25 14
Bpu10I CC'TnA_GC
Cuts at: 926 926
Size: 4727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 630 1903 2782 3274 3572 630
Size: 1273 879 492 298 1785
Fragments arranged by size:
1785 1273 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 593 1530 4431 593
Size: 937 2901 889
Fragments arranged by size:
2901 937 889
BsaAI yAC'GTr
Cuts at: 2080 2080
Size: 4727
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 2158 2406 3108 4604 2158
Size: 248 702 1496 2281
Fragments arranged by size:
2281 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1916 2437 3115 3389 3536 3947 1916
Size: 521 678 274 147 411 2696
Fragments arranged by size:
2696 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 636 652 720 805 820 1216 636
Size: 16 68 85 15 396 4147
Fragments arranged by size:
4147 396 85 68 16 15
BseYI C'CCAG_C
Cuts at: 119 158 238 919 1014 1153 1305 1336 1486
Size: 39 80 681 95 139 152 31 150
Cuts at: 1486 2152 2910 3487 3983 119
Size: 666 758 577 496 863
Fragments arranged by size:
863 758 681 666 577 496 152 150
139 95 80 39 31
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3523 104
Size: 2165 46 1208 1308
Fragments arranged by size:
2165 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 990 1741 2519 2709 3501 990
Size: 751 778 190 792 2216
Fragments arranged by size:
2216 792 778 751 190
Bsp1286I G_dGCh'C
Cuts at: 719 990 1030 1040 1081 1148 1189 1337 1430
Size: 271 40 10 41 67 41 148 93
Cuts at: 1430 1546 1597 1705 1741 2352 2445 2519 2709
Size: 116 51 108 36 611 93 74 190
Cuts at: 2709 2771 3501 719
Size: 62 730 1945
Fragments arranged by size:
1945 730 611 271 190 148 116 108
93 93 74 67 62 51 41 41
40 36 10
BspEI T'CCGG_A
Cuts at: 3947 3947
Size: 4727
BspHI T'CATG_A
Cuts at: 3903 3903
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 355 693 827 1777 1991 2293 2674 355
Size: 338 134 950 214 302 381 2408
Fragments arranged by size:
2408 950 381 338 302 214 134
BsrBI CCG'CTC
Cuts at: 50 930 3019 3073 4557 4722 50
Size: 880 2089 54 1484 165 55
Fragments arranged by size:
2089 1484 880 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2639 2639
Size: 4727
BsrFI r'CCGG_y
Cuts at: 465 1508 1555 2725 2906 3115 465
Size: 1043 47 1170 181 209 2077
Fragments arranged by size:
2077 1170 1043 209 181 47
BsrGI T'GTAC_A
Cuts at: 947 1055 947
Size: 108 4619
BssSI C'ACGA_G
Cuts at: 2998 3356 2998
Size: 358 4369
BstAPI GCAn_nnn'nTGC
Cuts at: 2104 2104
Size: 4727
BstBI TT'CG_AA
Cuts at: 3088 3088
Size: 4727
BstEII G'GTnAC_C
Cuts at: 1511 1511
Size: 4727
BstXI CCAn_nnnn'nTGG
Cuts at: 433 1274 433
Size: 841 3886
BstYI r'GATC_y
Cuts at: 1 1752 2219 2242 2577 2823 3835 3921 3933
Size: 1751 467 23 335 246 1012 86 12
Cuts at: 3933 4279 1
Size: 346 449
Fragments arranged by size:
1751 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1072 1072
Size: 4727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 1970 1970
Size: 4727
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3291 2433
Size: 858 3869
EaeI y'GGCC_r
Cuts at: 374 1082 1162 1213 1506 1553 2312 2486 2877
Size: 708 80 51 293 47 759 174 391
Cuts at: 2877 2904 4249 374
Size: 27 1345 852
Fragments arranged by size:
1345 852 759 708 391 293 174 80
51 47 27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 269 2750 2960 269
Size: 2481 210 2036
Fragments arranged by size:
2481 2036 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1657 1679 2935 3245 3391 4537 1657
Size: 22 1256 310 146 1146 1847
Fragments arranged by size:
1847 1256 1146 310 146 22
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 181 301 553 577 691 768 885 892
Size: 48 120 252 24 114 77 117 7
Cuts at: 892 987 1030 1044 1069 1351 1538 2552 2984
Size: 95 43 14 25 282 187 1014 432
Cuts at: 2984 3731 3999 133
Size: 747 268 861
Fragments arranged by size:
1014 861 747 432 282 268 252 187
120 117 114 95 77 48 43 25
24 14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 949 1418 949
Size: 469 4258
EcoO109I rG'GnC_Cy
Cuts at: 715 743 1018 1353 1426 1427 1648 1866 715
Size: 28 275 335 73 1 221 218 3576
Fragments arranged by size:
3576 335 275 221 218 73 28 1
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1173 2746 1173
Size: 1573 3154
FseI GG_CCGG'CC
Cuts at: 1559 1559
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 525 2161 2409 3431 4410 525
Size: 1636 248 1022 979 842
Fragments arranged by size:
1636 1022 979 842 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2569 2599 2601 2631 2569
Size: 30 2 30 4665
Fragments arranged by size:
4665 30 30 2
HincII GTy'rAC
Cuts at: 396 1766 396
Size: 1370 3357
HindIII A'AGCT_T
Cuts at: 565 565
Size: 4727
KasI G'GCGC_C
Cuts at: 2157 2405 2157
Size: 248 4479
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3398 3582 3398
Size: 184 4543
MscI TGG'CCA
Cuts at: 2488 2488
Size: 4727
MslI CAynn'nnrTG
Cuts at: 356 2716 2843 356
Size: 2360 127 2240
Fragments arranged by size:
2360 2240 127
MspA1I CmG'CkG
Cuts at: 962 1061 1286 1405 1838 2152 2512 3525 3770
Size: 99 225 119 433 314 360 1013 245
Cuts at: 3770 962
Size: 1919
Fragments arranged by size:
1919 1013 433 360 314 245 225 119
99
NaeI GCC'GGC
Cuts at: 467 1557 2908 467
Size: 1090 1351 2286
Fragments arranged by size:
2286 1351 1090
NarI GG'CG_CC
Cuts at: 2158 2406 2158
Size: 248 4479
NgoMIV G'CCGG_C
Cuts at: 465 1555 2906 465
Size: 1090 1351 2286
Fragments arranged by size:
2286 1351 1090
NspI r_CATG'y
Cuts at: 337 555 1780 2280 3187 337
Size: 218 1225 500 907 1877
Fragments arranged by size:
1877 1225 907 500 218
PasI CC'CwG_GG
Cuts at: 605 1414 1431 1499 605
Size: 809 17 68 3833
Fragments arranged by size:
3833 809 68 17
PciI A'CATG_T
Cuts at: 3183 3183
Size: 4727
PflMI CCAn_nnn'nTGG
Cuts at: 372 1160 1677 372
Size: 788 517 3422
Fragments arranged by size:
3422 788 517
PfoI T'CCnGG_A
Cuts at: 215 1442 215
Size: 1227 3500
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4695
PpuMI rG'GwC_Cy
Cuts at: 743 1018 1353 1648 743
Size: 275 335 295 3822
Fragments arranged by size:
3822 335 295 275
PshAI GACnn'nnGTC
Cuts at: 1378 1378
Size: 4727
PspOMI G'GGCC_C
Cuts at: 715 1426 715
Size: 711 4016
PspXI vC'TCGA_Gb
Cuts at: 687 687
Size: 4727
PstI C_TGCA'G
Cuts at: 304 584 704 961 1097 1285 1606 1774 2459
Size: 280 120 257 136 188 321 168 685
Cuts at: 2459 304
Size: 2572
Fragments arranged by size:
2572 685 321 280 257 188 168 136
120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 1061 1286 1405 2152 2512 1061
Size: 225 119 747 360 3276
Fragments arranged by size:
3276 747 360 225 119
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SanDI GG'GwC_CC
Cuts at: 1018 1018
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 269 2750 2960 269
Size: 2481 210 2036
Fragments arranged by size:
2481 2036 210
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SfcI C'TryA_G
Cuts at: 33 300 580 700 957 1093 1281 1602 1770
Size: 267 280 120 257 136 188 321 168
Cuts at: 1770 2455 3448 3639 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 321 280 267 257 191
188 168 136 120
SfoI GGC'GCC
Cuts at: 2159 2407 2159
Size: 248 4479
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1638 1749 1638
Size: 111 4616
SmlI C'TyrA_G
Cuts at: 645 687 1882 2797 3289 3551 645
Size: 42 1195 915 492 262 1821
Fragments arranged by size:
1821 1195 915 492 262 42
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 337 1780 2280 337
Size: 1443 500 2784
Fragments arranged by size:
2784 1443 500
StuI AGG'CCT
Cuts at: 1346 1346
Size: 4727
StyI C'CwwG_G
Cuts at: 1861 2215 1861
Size: 354 4373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 969 973 1082 1665 2310 2443 2902 3111 969
Size: 4 109 583 645 133 459 209 2585
Fragments arranged by size:
2585 645 583 459 209 133 109 4
TatI w'GTAC_w
Cuts at: 584 947 1055 4242 584
Size: 363 108 3187 1069
Fragments arranged by size:
3187 1069 363 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 735 767 4244 4276 735
Size: 32 3477 32 1186
Fragments arranged by size:
3477 1186 32 32
Tth111I GACn'n_nGTC
Cuts at: 175 766 783 2524 175
Size: 591 17 1741 2378
Fragments arranged by size:
2378 1741 591 17
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1011 2222 1011
Size: 1211 3516
XhoI C'TCGA_G
Cuts at: 687 687
Size: 4727
XmaI C'CCGG_G
Cuts at: 1636 1747 1636
Size: 111 4616
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI