Restriction map for
pF1KB5937
(Circular) (Six-base) MAPSORT of: pF1KB5937.seq Check: 6344 from: 1 to: 4637
pF1KB5937 4637 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1675 1675
Size: 4637
Acc65I G'GTAC_C
Cuts at: 1653 1653
Size: 4637
AclI AA'CG_TT
Cuts at: 4477 4477
Size: 4637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 402 626 637 1405 2462 2894 3641 3909
Size: 179 224 11 768 1057 432 747 268
Cuts at: 3909 223
Size: 951
Fragments arranged by size:
1057 951 768 747 432 268 224 179
11
AflIII A'CryG_T
Cuts at: 1460 1989 3093 4070 1460
Size: 529 1104 977 2027
Fragments arranged by size:
2027 1104 977 529
AgeI A'CCGG_T
Cuts at: 3025 3025
Size: 4637
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 154 817 849 122
Size: 32 663 32 3910
Fragments arranged by size:
3910 663 32 32
AlwNI CAG_nnn'CTG
Cuts at: 246 1942 3509 246
Size: 1696 1567 1374
Fragments arranged by size:
1696 1567 1374
ApaI G_GGCC'C
Cuts at: 476 476
Size: 4637
ApaLI G'TGCA_C
Cuts at: 3407 3407
Size: 4637
ApoI r'AATT_y
Cuts at: 1641 3990 4246 1641
Size: 2349 256 2032
Fragments arranged by size:
2349 2032 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4637
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
AvaI C'yCGr_G
Cuts at: 320 476 1410 1453 1657 320
Size: 156 934 43 204 3300
Fragments arranged by size:
3300 934 204 156 43
BamHI G'GATC_C
Cuts at: 1662 1662
Size: 4637
BanI G'GyrC_C
Cuts at: 223 1653 2067 2315 2350 223
Size: 1430 414 248 35 2510
Fragments arranged by size:
2510 1430 414 248 35
BanII G_rGCy'C
Cuts at: 476 688 871 1352 1651 2681 476
Size: 212 183 481 299 1030 2432
Fragments arranged by size:
2432 1030 481 299 212 183
BbeI G_GCGC'C
Cuts at: 227 2071 2319 227
Size: 1844 248 2545
Fragments arranged by size:
2545 1844 248
BbsI GAAGACnn'nnnn_
Cuts at: 625 1410 625
Size: 785 3852
BbvCI CC'TCA_GC
Cuts at: 583 583
Size: 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 298 332 298
Size: 34 4603
BciVI GTATCCnnnnn_n'
Cuts at: 2535 3296 4637 2535
Size: 761 1341 2535
Fragments arranged by size:
2535 1341 761
BclI T'GATC_A
Cuts at: 1505 2157 1505
Size: 652 3985
BglI GCCn_nnn'nGGC
Cuts at: 184 1446 184
Size: 1262 3375
BglII A'GATC_T
Cuts at: 815 2152 4189 815
Size: 1337 2037 1263
Fragments arranged by size:
2037 1337 1263
BlpI GC'TnA_GC
Cuts at: 191 682 1749 191
Size: 491 1067 3079
Fragments arranged by size:
3079 1067 491
Bme1580I G_kGCm'C
Cuts at: 476 2262 2355 3411 476
Size: 1786 93 1056 1702
Fragments arranged by size:
1786 1702 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 297 395 636 798 1849 1930 2265 4409 297
Size: 98 241 162 1051 81 335 2144 525
Fragments arranged by size:
2144 1051 525 335 241 162 98 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 714 1628 3004 714
Size: 914 1376 2347
Fragments arranged by size:
2347 1376 914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 511 684 844 1510 169
Size: 342 173 160 666 3296
Fragments arranged by size:
3296 666 342 173 160
Bpu10I CC'TnA_GC
Cuts at: 583 583
Size: 4637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1813 2692 3184 3482 775
Size: 1038 879 492 298 1930
Fragments arranged by size:
1930 1038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1180 1255 4341 1180
Size: 75 3086 1476
Fragments arranged by size:
3086 1476 75
BsaAI yAC'GTr
Cuts at: 1990 1990
Size: 4637
BsaBI GATnn'nnATC
Cuts at: 6 2151 4188 6
Size: 2145 2037 455
Fragments arranged by size:
2145 2037 455
BsaHI Gr'CG_yC
Cuts at: 154 224 887 1196 2068 2316 3018 4514 154
Size: 70 663 309 872 248 702 1496 277
Fragments arranged by size:
1496 872 702 663 309 277 248 70
BsaWI w'CCGG_w
Cuts at: 1146 1178 1826 2347 3025 3299 3446 3857 1146
Size: 32 648 521 678 274 147 411 1926
Fragments arranged by size:
1926 678 648 521 411 274 147 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 122 152 817 847 122
Size: 30 665 30 3912
Fragments arranged by size:
3912 665 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 588 325
Size: 263 4374
BseYI C'CCAG_C
Cuts at: 195 246 558 902 912 1299 1351 2062 2820
Size: 51 312 344 10 387 52 711 758
Cuts at: 2820 3397 3893 195
Size: 577 496 939
Fragments arranged by size:
939 758 711 577 496 387 344 312
52 51 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 4637
BsiEI CG_ry'CG
Cuts at: 104 1170 2179 2225 3433 104
Size: 1066 1009 46 1208 1308
Fragments arranged by size:
1308 1208 1066 1009 46
BsiHKAI G_wGCw'C
Cuts at: 209 871 1651 2429 2619 3411 209
Size: 662 780 778 190 792 1435
Fragments arranged by size:
1435 792 780 778 662 190
BsmI GAATG_Cn'
Cuts at: 356 356
Size: 4637
Bsp1286I G_dGCh'C
Cuts at: 209 476 688 871 1352 1651 2262 2355 2429
Size: 267 212 183 481 299 611 93 74
Cuts at: 2429 2619 2681 3411 209
Size: 190 62 730 1435
Fragments arranged by size:
1435 730 611 481 299 267 212 190
183 93 74 62
BspEI T'CCGG_A
Cuts at: 1178 3857 1178
Size: 2679 1958
BspHI T'CATG_A
Cuts at: 3813 3813
Size: 4637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1565 1687 1901 2203 2584 1565
Size: 122 214 302 381 3618
Fragments arranged by size:
3618 381 302 214 122
BsrBI CCG'CTC
Cuts at: 50 800 2929 2983 4467 4632 50
Size: 750 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 750 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1469 2549 1469
Size: 1080 3557
BsrFI r'CCGG_y
Cuts at: 719 2635 2816 3025 719
Size: 1916 181 209 2331
Fragments arranged by size:
2331 1916 209 181
BssSI C'ACGA_G
Cuts at: 2908 3266 2908
Size: 358 4279
BstAPI GCAn_nnn'nTGC
Cuts at: 2014 2014
Size: 4637
BstBI TT'CG_AA
Cuts at: 351 2998 351
Size: 2647 1990
BstXI CCAn_nnnn'nTGG
Cuts at: 1206 1206
Size: 4637
BstYI r'GATC_y
Cuts at: 1 815 948 1662 2129 2152 2487 2733 3745
Size: 814 133 714 467 23 335 246 1012
Cuts at: 3745 3831 3843 4189 1
Size: 86 12 346 449
Fragments arranged by size:
1012 814 714 467 449 346 335 246
133 86 23 12
BtgI C'CryG_G
Cuts at: 107 750 1442 107
Size: 643 692 3302
Fragments arranged by size:
3302 692 643
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2587 2768 2931 2587
Size: 181 163 4293
Fragments arranged by size:
4293 181 163
BtsI GCAGTG_nn'
Cuts at: 648 1880 648
Size: 1232 3405
DraI TTT'AAA
Cuts at: 1636 1636
Size: 4637
DraIII CAC_nnn'GTG
Cuts at: 1551 1551
Size: 4637
DrdI GACnn_nn'nnGTC
Cuts at: 2343 3201 2343
Size: 858 3779
EaeI y'GGCC_r
Cuts at: 771 2222 2396 2787 2814 4159 771
Size: 1451 174 391 27 1345 1249
Fragments arranged by size:
1451 1345 1249 391 174 27
EagI C'GGCC_G
Cuts at: 2222 2222
Size: 4637
EarI CTCTTCn'nnn_
Cuts at: 226 583 898 2660 2870 226
Size: 357 315 1762 210 1993
Fragments arranged by size:
1993 1762 357 315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 142 1255 2845 3155 3301 4447 127
Size: 15 1113 1590 310 146 1146 317
Fragments arranged by size:
1590 1146 1113 317 310 146 15
EcoICRI GAG'CTC
Cuts at: 869 1649 869
Size: 780 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 223 402 511 626 637 684 844 1405
Size: 54 179 109 115 11 47 160 561
Cuts at: 1405 1510 2462 2894 3641 3909 169
Size: 105 952 432 747 268 897
Fragments arranged by size:
952 897 747 561 432 268 179 160
115 109 105 54 47 11
EcoO109I rG'GnC_Cy
Cuts at: 369 473 960 1479 1607 1776 369
Size: 104 487 519 128 169 3230
Fragments arranged by size:
3230 519 487 169 128 104
EcoRI G'AATT_C
Cuts at: 1641 1641
Size: 4637
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1155 2656 1155
Size: 1501 3136
FspI TGC'GCA
Cuts at: 2418 2418
Size: 4637
HaeII r_GCGC'y
Cuts at: 179 227 247 1106 1497 2071 2319 3341 4320
Size: 48 20 859 391 574 248 1022 979
Cuts at: 4320 179
Size: 496
Fragments arranged by size:
1022 979 859 574 496 391 248 48
20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 993 1025 1447 1479 2479 2509 2511 2541 993
Size: 32 422 32 1000 30 2 30 3089
Fragments arranged by size:
3089 1000 422 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1676 1676
Size: 4637
KasI G'GCGC_C
Cuts at: 223 2067 2315 223
Size: 1844 248 2545
Fragments arranged by size:
2545 1844 248
KpnI G_GTAC'C
Cuts at: 1657 1657
Size: 4637
MluI A'CGCG_T
Cuts at: 4070 4070
Size: 4637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 458 834 1383 3308 3492 458
Size: 376 549 1925 184 1603
Fragments arranged by size:
1925 1603 549 376 184
MscI TGG'CCA
Cuts at: 2398 2398
Size: 4637
MslI CAynn'nnrTG
Cuts at: 841 1360 2626 2753 841
Size: 519 1266 127 2725
Fragments arranged by size:
2725 1266 519 127
MspA1I CmG'CkG
Cuts at: 519 531 770 786 955 990 1748 2062 2422
Size: 12 239 16 169 35 758 314 360
Cuts at: 2422 3435 3680 519
Size: 1013 245 1476
Fragments arranged by size:
1476 1013 758 360 314 245 239 169
35 16 12
NaeI GCC'GGC
Cuts at: 2818 2818
Size: 4637
NarI GG'CG_CC
Cuts at: 224 2068 2316 224
Size: 1844 248 2545
Fragments arranged by size:
2545 1844 248
NcoI C'CATG_G
Cuts at: 107 1442 107
Size: 1335 3302
NgoMIV G'CCGG_C
Cuts at: 2816 2816
Size: 4637
NspI r_CATG'y
Cuts at: 1690 2190 3097 1690
Size: 500 907 3230
Fragments arranged by size:
3230 907 500
PciI A'CATG_T
Cuts at: 3093 3093
Size: 4637
PflMI CCAn_nnn'nTGG
Cuts at: 216 287 440 1269 216
Size: 71 153 829 3584
Fragments arranged by size:
3584 829 153 71
PfoI T'CCnGG_A
Cuts at: 821 1529 821
Size: 708 3929
PmeI GTTT'AAAC
Cuts at: 1636 1636
Size: 4637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 122 154 817 849 3776 3808 122
Size: 32 663 32 2927 32 951
Fragments arranged by size:
2927 951 663 32 32 32
PspOMI G'GGCC_C
Cuts at: 472 472
Size: 4637
PstI C_TGCA'G
Cuts at: 968 1562 1684 2369 968
Size: 594 122 685 3236
Fragments arranged by size:
3236 685 594 122
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4637
PvuII CAG'CTG
Cuts at: 531 2062 2422 531
Size: 1531 360 2746
Fragments arranged by size:
2746 1531 360
RsrII CG'GwC_CG
Cuts at: 2832 2832
Size: 4637
SacI G_AGCT'C
Cuts at: 871 1651 871
Size: 780 3857
SalI G'TCGA_C
Cuts at: 1674 1674
Size: 4637
SapI GCTCTTCn'nnn_
Cuts at: 226 2660 2870 226
Size: 2434 210 1993
Fragments arranged by size:
2434 1993 210
SbfI CC_TGCA'GG
Cuts at: 1684 1684
Size: 4637
ScaI AGT'ACT
Cuts at: 1156 4154 1156
Size: 2998 1639
SfcI C'TryA_G
Cuts at: 33 964 1558 1680 2365 3358 3549 33
Size: 931 594 122 685 993 191 1121
Fragments arranged by size:
1121 993 931 685 594 191 122
SfoI GGC'GCC
Cuts at: 225 2069 2317 225
Size: 1844 248 2545
Fragments arranged by size:
2545 1844 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
SmaI CCC'GGG
Cuts at: 322 478 1659 322
Size: 156 1181 3300
Fragments arranged by size:
3300 1181 156
SmlI C'TyrA_G
Cuts at: 790 1792 2707 3199 3461 790
Size: 1002 915 492 262 1966
Fragments arranged by size:
1966 1002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4637
SphI G_CATG'C
Cuts at: 1690 2190 1690
Size: 500 4137
StuI AGG'CCT
Cuts at: 1382 1382
Size: 4637
StyI C'CwwG_G
Cuts at: 107 1256 1377 1442 1771 2125 107
Size: 1149 121 65 329 354 2619
Fragments arranged by size:
2619 1149 354 329 121 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 263 618 926 1383 1455 2220 2353 2812 3021
Size: 355 308 457 72 765 133 459 209
Cuts at: 3021 263
Size: 1879
Fragments arranged by size:
1879 765 459 457 355 308 209 133
72
TatI w'GTAC_w
Cuts at: 1154 4152 1154
Size: 2998 1639
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4605
Tth111I GACn'n_nGTC
Cuts at: 982 2434 982
Size: 1452 3185
XbaI T'CTAG_A
Cuts at: 1668 1668
Size: 4637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 408 2132 408
Size: 1724 2913
XmaI C'CCGG_G
Cuts at: 320 476 1657 320
Size: 156 1181 3300
Fragments arranged by size:
3300 1181 156
XmnI GAAnn'nnTTC
Cuts at: 1138 1138
Size: 4637
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI