Restriction map for
pF1KB5939
(Circular) (Six-base) MAPSORT of: pF1KB5939.seq Check: 3746 from: 1 to: 4637
pF1KB5939 4637 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1675 1675
Size: 4637
Acc65I G'GTAC_C
Cuts at: 1653 1653
Size: 4637
AclI AA'CG_TT
Cuts at: 4477 4477
Size: 4637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 405 514 1048 2462 2894 3641 3909 337
Size: 68 109 534 1414 432 747 268 1065
Fragments arranged by size:
1414 1065 747 534 432 268 109 68
AflIII A'CryG_T
Cuts at: 680 1989 3093 4070 680
Size: 1309 1104 977 1247
Fragments arranged by size:
1309 1247 1104 977
AgeI A'CCGG_T
Cuts at: 3025 3025
Size: 4637
AhdI GACnn_n'nnGTC
Cuts at: 600 600
Size: 4637
AlwNI CAG_nnn'CTG
Cuts at: 1185 1942 3509 1185
Size: 757 1567 2313
Fragments arranged by size:
2313 1567 757
ApaI G_GGCC'C
Cuts at: 511 511
Size: 4637
ApaLI G'TGCA_C
Cuts at: 1417 3407 1417
Size: 1990 2647
ApoI r'AATT_y
Cuts at: 1510 1641 3990 4246 1510
Size: 131 2349 256 1901
Fragments arranged by size:
2349 1901 256 131
AseI AT'TA_AT
Cuts at: 20 20
Size: 4637
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
AvaI C'yCGr_G
Cuts at: 166 1012 1558 1657 166
Size: 846 546 99 3146
Fragments arranged by size:
3146 846 546 99
BamHI G'GATC_C
Cuts at: 1662 1662
Size: 4637
BanI G'GyrC_C
Cuts at: 1653 2067 2315 2350 1653
Size: 414 248 35 3940
Fragments arranged by size:
3940 414 248 35
BanII G_rGCy'C
Cuts at: 511 1651 2681 511
Size: 1140 1030 2467
Fragments arranged by size:
2467 1140 1030
BbeI G_GCGC'C
Cuts at: 2071 2319 2071
Size: 248 4389
BbsI GAAGACnn'nnnn_
Cuts at: 1247 1604 1247
Size: 357 4280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4603
BciVI GTATCCnnnnn_n'
Cuts at: 2535 3296 4637 2535
Size: 761 1341 2535
Fragments arranged by size:
2535 1341 761
BclI T'GATC_A
Cuts at: 1605 2157 1605
Size: 552 4085
BglII A'GATC_T
Cuts at: 2152 4189 2152
Size: 2037 2600
BlpI GC'TnA_GC
Cuts at: 1749 1749
Size: 4637
Bme1580I G_kGCm'C
Cuts at: 511 1045 1421 2262 2355 3411 511
Size: 534 376 841 93 1056 1737
Fragments arranged by size:
1737 1056 841 534 376 93
BmgBI CAC'GTC
Cuts at: 183 597 681 1245 183
Size: 414 84 564 3575
Fragments arranged by size:
3575 564 414 84
BmrI ACTGGGnnnn_n'
Cuts at: 1206 1345 1849 1930 2265 4409 1206
Size: 139 504 81 335 2144 1434
Fragments arranged by size:
2144 1434 504 335 139 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 624 3004 624
Size: 2380 2257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1168 1537 1087
Size: 81 369 4187
Fragments arranged by size:
4187 369 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 1813 2692 3184 3482 1420
Size: 393 879 492 298 2575
Fragments arranged by size:
2575 879 492 393 298
BsaI GGTCTCn'nnnn_
Cuts at: 4341 4341
Size: 4637
BsaAI yAC'GTr
Cuts at: 1990 1990
Size: 4637
BsaBI GATnn'nnATC
Cuts at: 6 1553 2151 4188 6
Size: 1547 598 2037 455
Fragments arranged by size:
2037 1547 598 455
BsaHI Gr'CG_yC
Cuts at: 161 356 2068 2316 3018 4514 161
Size: 195 1712 248 702 1496 284
Fragments arranged by size:
1712 1496 702 284 248 195
BsaWI w'CCGG_w
Cuts at: 1826 2347 3025 3299 3446 3857 1826
Size: 521 678 274 147 411 2606
Fragments arranged by size:
2606 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 670 700 670
Size: 30 4607
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 187 379 1084 1087 1117 1594 184
Size: 3 192 705 3 30 477 3227
Fragments arranged by size:
3227 705 477 192 30 3 3
BseYI C'CCAG_C
Cuts at: 307 380 2062 2820 3397 3893 307
Size: 73 1682 758 577 496 1051
Fragments arranged by size:
1682 1051 758 577 496 73
BsiEI CG_ry'CG
Cuts at: 104 1049 2179 2225 3433 104
Size: 945 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 945 46
BsiHKAI G_wGCw'C
Cuts at: 1421 1651 2429 2619 3411 1421
Size: 230 778 190 792 2647
Fragments arranged by size:
2647 792 778 230 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1336 1336
Size: 4637
Bsp1286I G_dGCh'C
Cuts at: 511 1045 1421 1651 2262 2355 2429 2619 2681
Size: 534 376 230 611 93 74 190 62
Cuts at: 2681 3411 511
Size: 730 1737
Fragments arranged by size:
1737 730 611 534 376 230 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3857 3857
Size: 4637
BspHI T'CATG_A
Cuts at: 3813 3813
Size: 4637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 245 788 1687 1901 2203 2584 245
Size: 543 899 214 302 381 2298
Fragments arranged by size:
2298 899 543 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1523 2929 2983 4467 4632 50
Size: 1473 1406 54 1484 165 55
Fragments arranged by size:
1484 1473 1406 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2549 2549
Size: 4637
BsrFI r'CCGG_y
Cuts at: 2635 2816 3025 2635
Size: 181 209 4247
Fragments arranged by size:
4247 209 181
BssHII G'CGCG_C
Cuts at: 153 153
Size: 4637
BssSI C'ACGA_G
Cuts at: 2908 3266 2908
Size: 358 4279
BstAPI GCAn_nnn'nTGC
Cuts at: 624 2014 624
Size: 1390 3247
BstBI TT'CG_AA
Cuts at: 2998 2998
Size: 4637
BstEII G'GTnAC_C
Cuts at: 765 765
Size: 4637
BstXI CCAn_nnnn'nTGG
Cuts at: 570 1401 570
Size: 831 3806
BstYI r'GATC_y
Cuts at: 1 962 1662 2129 2152 2487 2733 3745 3831
Size: 961 700 467 23 335 246 1012 86
Cuts at: 3831 3843 4189 1
Size: 12 346 449
Fragments arranged by size:
1012 961 700 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 137 359 1291 137
Size: 222 932 3483
Fragments arranged by size:
3483 932 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1356 2587 2768 2931 1356
Size: 1231 181 163 3062
Fragments arranged by size:
3062 1231 181 163
BtsI GCAGTG_nn'
Cuts at: 270 1302 1880 270
Size: 1032 578 3027
Fragments arranged by size:
3027 1032 578
ClaI AT'CG_AT
Cuts at: 644 644
Size: 4637
DraI TTT'AAA
Cuts at: 1636 1636
Size: 4637
DrdI GACnn_nn'nnGTC
Cuts at: 1465 2343 3201 1465
Size: 878 858 2901
Fragments arranged by size:
2901 878 858
EaeI y'GGCC_r
Cuts at: 134 140 267 344 1046 1527 2222 2396 2787
Size: 6 127 77 702 481 695 174 391
Cuts at: 2787 2814 4159 134
Size: 27 1345 612
Fragments arranged by size:
1345 702 695 612 481 391 174 127
77 27 6
EagI C'GGCC_G
Cuts at: 1046 2222 1046
Size: 1176 3461
EarI CTCTTCn'nnn_
Cuts at: 1031 2660 2870 1031
Size: 1629 210 2798
Fragments arranged by size:
2798 1629 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2845 3155 3301 4447 2845
Size: 310 146 1146 3035
Fragments arranged by size:
3035 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1649 1649
Size: 4637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 405 514 1048 1087 1168 1537 2462 2894
Size: 68 109 534 39 81 369 925 432
Cuts at: 2894 3641 3909 337
Size: 747 268 1065
Fragments arranged by size:
1065 925 747 534 432 369 268 109
81 68 39
EcoNI CCTnn'n_nnAGG
Cuts at: 1030 1069 1030
Size: 39 4598
EcoO109I rG'GnC_Cy
Cuts at: 1563 1776 1563
Size: 213 4424
EcoRI G'AATT_C
Cuts at: 1641 1641
Size: 4637
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4637
FspI TGC'GCA
Cuts at: 2418 2418
Size: 4637
HaeII r_GCGC'y
Cuts at: 220 2071 2319 3341 4320 220
Size: 1851 248 1022 979 537
Fragments arranged by size:
1851 1022 979 537 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 670 702 848 880 2479 2509 2511
Size: 32 52 32 146 32 1599 30 2
Cuts at: 2511 2541 586
Size: 30 2682
Fragments arranged by size:
2682 1599 146 52 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1676 1676
Size: 4637
KasI G'GCGC_C
Cuts at: 2067 2315 2067
Size: 248 4389
KpnI G_GTAC'C
Cuts at: 1657 1657
Size: 4637
MluI A'CGCG_T
Cuts at: 4070 4070
Size: 4637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 584 695 3308 3492 584
Size: 111 2613 184 1729
Fragments arranged by size:
2613 1729 184 111
MscI TGG'CCA
Cuts at: 269 346 1529 2398 269
Size: 77 1183 869 2508
Fragments arranged by size:
2508 1183 869 77
MslI CAynn'nnrTG
Cuts at: 2626 2753 2626
Size: 127 4510
MspA1I CmG'CkG
Cuts at: 307 460 541 807 1748 2062 2422 3435 3680
Size: 153 81 266 941 314 360 1013 245
Cuts at: 3680 307
Size: 1264
Fragments arranged by size:
1264 1013 941 360 314 266 245 153
81
NaeI GCC'GGC
Cuts at: 2818 2818
Size: 4637
NarI GG'CG_CC
Cuts at: 2068 2316 2068
Size: 248 4389
NcoI C'CATG_G
Cuts at: 1291 1291
Size: 4637
NgoMIV G'CCGG_C
Cuts at: 2816 2816
Size: 4637
NspI r_CATG'y
Cuts at: 1690 2190 3097 1690
Size: 500 907 3230
Fragments arranged by size:
3230 907 500
PciI A'CATG_T
Cuts at: 3093 3093
Size: 4637
PflMI CCAn_nnn'nTGG
Cuts at: 186 1297 186
Size: 1111 3526
PfoI T'CCnGG_A
Cuts at: 1145 1514 1145
Size: 369 4268
PmeI GTTT'AAAC
Cuts at: 1636 1636
Size: 4637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4605
PpuMI rG'GwC_Cy
Cuts at: 1563 1563
Size: 4637
PspOMI G'GGCC_C
Cuts at: 507 507
Size: 4637
PspXI vC'TCGA_Gb
Cuts at: 1012 1012
Size: 4637
PstI C_TGCA'G
Cuts at: 546 1684 2369 546
Size: 1138 685 2814
Fragments arranged by size:
2814 1138 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4637
PvuII CAG'CTG
Cuts at: 460 541 807 2062 2422 460
Size: 81 266 1255 360 2675
Fragments arranged by size:
2675 1255 360 266 81
RsrII CG'GwC_CG
Cuts at: 2832 2832
Size: 4637
SacI G_AGCT'C
Cuts at: 1651 1651
Size: 4637
SalI G'TCGA_C
Cuts at: 1674 1674
Size: 4637
SapI GCTCTTCn'nnn_
Cuts at: 2660 2870 2660
Size: 210 4427
SbfI CC_TGCA'GG
Cuts at: 1684 1684
Size: 4637
ScaI AGT'ACT
Cuts at: 4154 4154
Size: 4637
SfcI C'TryA_G
Cuts at: 33 542 1680 2365 3358 3549 33
Size: 509 1138 685 993 191 1121
Fragments arranged by size:
1138 1121 993 685 509 191
SfoI GGC'GCC
Cuts at: 2069 2317 2069
Size: 248 4389
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
SmaI CCC'GGG
Cuts at: 1659 1659
Size: 4637
SmlI C'TyrA_G
Cuts at: 1012 1435 1558 1792 2707 3199 3461 1012
Size: 423 123 234 915 492 262 2188
Fragments arranged by size:
2188 915 492 423 262 234 123
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4637
SphI G_CATG'C
Cuts at: 1690 2190 1690
Size: 500 4137
StyI C'CwwG_G
Cuts at: 259 347 446 551 770 1291 1771 2125 259
Size: 88 99 105 219 521 480 354 2771
Fragments arranged by size:
2771 521 480 354 219 105 99 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2220 2353 2812 3021 2220
Size: 133 459 209 3836
Fragments arranged by size:
3836 459 209 133
TatI w'GTAC_w
Cuts at: 1085 4152 1085
Size: 3067 1570
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4605
Tth111I GACn'n_nGTC
Cuts at: 181 2434 181
Size: 2253 2384
XbaI T'CTAG_A
Cuts at: 1668 1668
Size: 4637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2132 2132
Size: 4637
XhoI C'TCGA_G
Cuts at: 1012 1558 1012
Size: 546 4091
XmaI C'CCGG_G
Cuts at: 1657 1657
Size: 4637
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsgI
BsiWI BsmI BsrGI BstZ17I Bsu36I CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI