Restriction map for
pF1KB5940
(Circular) (Six-base) MAPSORT of: pF1KB5940.seq Check: 6389 from: 1 to: 4733
pF1KB5940 4733 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 578 578
Size: 4733
AccI GT'mk_AC
Cuts at: 443 1771 443
Size: 1328 3405
Acc65I G'GTAC_C
Cuts at: 1749 1749
Size: 4733
AclI AA'CG_TT
Cuts at: 4573 4573
Size: 4733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1307 2558 2990 3737 4005 544
Size: 763 1251 432 747 268 1272
Fragments arranged by size:
1272 1251 763 747 432 268
AfeI AGC'GCT
Cuts at: 254 254
Size: 4733
AflII C'TTAA_G
Cuts at: 1126 1126
Size: 4733
AflIII A'CryG_T
Cuts at: 2085 3189 4166 2085
Size: 1104 977 2652
Fragments arranged by size:
2652 1104 977
AgeI A'CCGG_T
Cuts at: 1409 3121 1409
Size: 1712 3021
AleI CACnn'nnGTG
Cuts at: 581 581
Size: 4733
AlwNI CAG_nnn'CTG
Cuts at: 2038 3605 2038
Size: 1567 3166
ApaI G_GGCC'C
Cuts at: 1055 1055
Size: 4733
ApaLI G'TGCA_C
Cuts at: 3503 3503
Size: 4733
ApoI r'AATT_y
Cuts at: 414 815 866 952 1227 1436 1737 4086 4342
Size: 401 51 86 275 209 301 2349 256
Cuts at: 4342 414
Size: 805
Fragments arranged by size:
2349 805 401 301 275 256 209 86
51
AseI AT'TA_AT
Cuts at: 20 20
Size: 4733
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
AvaI C'yCGr_G
Cuts at: 158 497 1055 1753 158
Size: 339 558 698 3138
Fragments arranged by size:
3138 698 558 339
BamHI G'GATC_C
Cuts at: 1758 1758
Size: 4733
BanI G'GyrC_C
Cuts at: 225 1749 2163 2411 2446 225
Size: 1524 414 248 35 2512
Fragments arranged by size:
2512 1524 414 248 35
BanII G_rGCy'C
Cuts at: 1055 1387 1747 2777 1055
Size: 332 360 1030 3011
Fragments arranged by size:
3011 1030 360 332
BbeI G_GCGC'C
Cuts at: 229 2167 2415 229
Size: 1938 248 2547
Fragments arranged by size:
2547 1938 248
BbsI GAAGACnn'nnnn_
Cuts at: 608 608
Size: 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1104 1138 1104
Size: 34 4699
BciVI GTATCCnnnnn_n'
Cuts at: 2631 3392 4733 2631
Size: 761 1341 2631
Fragments arranged by size:
2631 1341 761
BclI T'GATC_A
Cuts at: 2253 2253
Size: 4733
BfrBI ATG'CAT
Cuts at: 1161 1161
Size: 4733
BglII A'GATC_T
Cuts at: 312 886 1641 2248 4285 312
Size: 574 755 607 2037 760
Fragments arranged by size:
2037 760 755 607 574
BlpI GC'TnA_GC
Cuts at: 1454 1845 1454
Size: 391 4342
Bme1580I G_kGCm'C
Cuts at: 1055 1354 2358 2451 3507 1055
Size: 299 1004 93 1056 2281
Fragments arranged by size:
2281 1056 1004 299 93
BmgBI CAC'GTC
Cuts at: 1100 1100
Size: 4733
BmrI ACTGGGnnnn_n'
Cuts at: 476 645 1945 2026 2361 4505 476
Size: 169 1300 81 335 2144 704
Fragments arranged by size:
2144 1300 704 335 169 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3100 3100
Size: 4733
Bpu10I CC'TnA_GC
Cuts at: 356 356
Size: 4733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1909 2788 3280 3578 1909
Size: 879 492 298 3064
Fragments arranged by size:
3064 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1052 4437 1052
Size: 3385 1348
BsaAI yAC'GTr
Cuts at: 2086 2086
Size: 4733
BsaBI GATnn'nnATC
Cuts at: 6 2247 4284 6
Size: 2241 2037 455
Fragments arranged by size:
2241 2037 455
BsaHI Gr'CG_yC
Cuts at: 212 226 2164 2412 3114 4610 212
Size: 14 1938 248 702 1496 335
Fragments arranged by size:
1938 1496 702 335 248 14
BsaWI w'CCGG_w
Cuts at: 267 324 1409 1922 2443 3121 3395 3542 3953
Size: 57 1085 513 521 678 274 147 411
Cuts at: 3953 267
Size: 1047
Fragments arranged by size:
1085 1047 678 521 513 411 274 147
57
BseYI C'CCAG_C
Cuts at: 1103 1415 2158 2916 3493 3989 1103
Size: 312 743 758 577 496 1847
Fragments arranged by size:
1847 758 743 577 496 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 1097 226
Size: 871 3862
BsiEI CG_ry'CG
Cuts at: 104 2275 2321 3529 104
Size: 2171 46 1208 1308
Fragments arranged by size:
2171 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 569 1008 1387 1747 2525 2715 3507 569
Size: 439 379 360 778 190 792 1795
Fragments arranged by size:
1795 792 778 439 379 360 190
Bsp1286I G_dGCh'C
Cuts at: 569 1008 1055 1354 1387 1747 2358 2451 2525
Size: 439 47 299 33 360 611 93 74
Cuts at: 2525 2715 2777 3507 569
Size: 190 62 730 1795
Fragments arranged by size:
1795 730 611 439 360 299 190 93
74 62 47 33
BspEI T'CCGG_A
Cuts at: 267 324 3953 267
Size: 57 3629 1047
Fragments arranged by size:
3629 1047 57
BspHI T'CATG_A
Cuts at: 3909 3909
Size: 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 1783 1997 2299 2680 578
Size: 1205 214 302 381 2631
Fragments arranged by size:
2631 1205 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3025 3079 4563 4728 50
Size: 2975 54 1484 165 55
Fragments arranged by size:
2975 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1247 2645 1247
Size: 1398 3335
BsrFI r'CCGG_y
Cuts at: 228 1409 2731 2912 3121 228
Size: 1181 1322 181 209 1840
Fragments arranged by size:
1840 1322 1181 209 181
BssSI C'ACGA_G
Cuts at: 3004 3362 3004
Size: 358 4375
BstAPI GCAn_nnn'nTGC
Cuts at: 2110 2110
Size: 4733
BstBI TT'CG_AA
Cuts at: 3094 3094
Size: 4733
BstEII G'GTnAC_C
Cuts at: 1107 1107
Size: 4733
BstXI CCAn_nnnn'nTGG
Cuts at: 187 1398 187
Size: 1211 3522
BstYI r'GATC_y
Cuts at: 1 312 886 1641 1758 2225 2248 2583 2829
Size: 311 574 755 117 467 23 335 246
Cuts at: 2829 3841 3927 3939 4285 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 755 574 467 449 346 335 311
246 117 86 23 12
BstZ17I GTA'TAC
Cuts at: 444 444
Size: 4733
BtgI C'CryG_G
Cuts at: 279 928 1420 279
Size: 649 492 3592
Fragments arranged by size:
3592 649 492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2683 2864 3027 2683
Size: 181 163 4389
Fragments arranged by size:
4389 181 163
BtsI GCAGTG_nn'
Cuts at: 1367 1976 1367
Size: 609 4124
DraI TTT'AAA
Cuts at: 699 1732 699
Size: 1033 3700
DrdI GACnn_nn'nnGTC
Cuts at: 2439 3297 2439
Size: 858 3875
EaeI y'GGCC_r
Cuts at: 173 1623 2318 2492 2883 2910 4255 173
Size: 1450 695 174 391 27 1345 651
Fragments arranged by size:
1450 1345 695 651 391 174 27
EagI C'GGCC_G
Cuts at: 2318 2318
Size: 4733
EarI CTCTTCn'nnn_
Cuts at: 1079 1380 2756 2966 1079
Size: 301 1376 210 2846
Fragments arranged by size:
2846 1376 301 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2941 3251 3397 4543 2941
Size: 310 146 1146 3131
Fragments arranged by size:
3131 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1385 1745 1385
Size: 360 4373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1307 2558 2990 3737 4005 544
Size: 763 1251 432 747 268 1272
Fragments arranged by size:
1272 1251 763 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 578 578
Size: 4733
EcoO109I rG'GnC_Cy
Cuts at: 275 1051 1052 1872 275
Size: 776 1 820 3136
Fragments arranged by size:
3136 820 776 1
EcoRI G'AATT_C
Cuts at: 1227 1737 1227
Size: 510 4223
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4733
FspI TGC'GCA
Cuts at: 2514 2514
Size: 4733
HaeII r_GCGC'y
Cuts at: 140 194 229 256 2167 2415 3437 4416 140
Size: 54 35 27 1911 248 1022 979 457
Fragments arranged by size:
1911 1022 979 457 248 54 35 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2575 2605 2607 2637 2575
Size: 30 2 30 4671
Fragments arranged by size:
4671 30 30 2
HincII GTy'rAC
Cuts at: 1772 1772
Size: 4733
KasI G'GCGC_C
Cuts at: 225 2163 2411 225
Size: 1938 248 2547
Fragments arranged by size:
2547 1938 248
KpnI G_GTAC'C
Cuts at: 1753 1753
Size: 4733
MluI A'CGCG_T
Cuts at: 4166 4166
Size: 4733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 656 3404 3588 656
Size: 2748 184 1801
Fragments arranged by size:
2748 1801 184
MscI TGG'CCA
Cuts at: 1625 2494 1625
Size: 869 3864
MslI CAynn'nnrTG
Cuts at: 581 1396 1709 2722 2849 581
Size: 815 313 1013 127 2465
Fragments arranged by size:
2465 1013 815 313 127
MspA1I CmG'CkG
Cuts at: 171 281 513 551 930 1143 1653 1844 2158
Size: 110 232 38 379 213 510 191 314
Cuts at: 2158 2518 3531 3776 171
Size: 360 1013 245 1128
Fragments arranged by size:
1128 1013 510 379 360 314 245 232
213 191 110 38
NaeI GCC'GGC
Cuts at: 230 2914 230
Size: 2684 2049
NarI GG'CG_CC
Cuts at: 226 2164 2412 226
Size: 1938 248 2547
Fragments arranged by size:
2547 1938 248
NcoI C'CATG_G
Cuts at: 1420 1420
Size: 4733
NdeI CA'TA_TG
Cuts at: 728 728
Size: 4733
NgoMIV G'CCGG_C
Cuts at: 228 2912 228
Size: 2684 2049
NsiI A_TGCA'T
Cuts at: 1163 1163
Size: 4733
NspI r_CATG'y
Cuts at: 835 1140 1786 2286 3193 835
Size: 305 646 500 907 2375
Fragments arranged by size:
2375 907 646 500 305
PasI CC'CwG_GG
Cuts at: 198 198
Size: 4733
PciI A'CATG_T
Cuts at: 3189 3189
Size: 4733
PflMI CCAn_nnn'nTGG
Cuts at: 222 612 1614 222
Size: 390 1002 3341
Fragments arranged by size:
3341 1002 390
PmeI GTTT'AAAC
Cuts at: 1732 1732
Size: 4733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4701
PpuMI rG'GwC_Cy
Cuts at: 275 275
Size: 4733
PspOMI G'GGCC_C
Cuts at: 1051 1051
Size: 4733
PstI C_TGCA'G
Cuts at: 294 1189 1780 2465 294
Size: 895 591 685 2562
Fragments arranged by size:
2562 895 685 591
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4733
PvuII CAG'CTG
Cuts at: 513 551 1143 1653 2158 2518 513
Size: 38 592 510 505 360 2728
Fragments arranged by size:
2728 592 510 505 360 38
RsrII CG'GwC_CG
Cuts at: 2928 2928
Size: 4733
SacI G_AGCT'C
Cuts at: 1387 1747 1387
Size: 360 4373
SacII CC_GC'GG
Cuts at: 282 931 282
Size: 649 4084
SalI G'TCGA_C
Cuts at: 1770 1770
Size: 4733
SanDI GG'GwC_CC
Cuts at: 275 275
Size: 4733
SapI GCTCTTCn'nnn_
Cuts at: 1079 2756 2966 1079
Size: 1677 210 2846
Fragments arranged by size:
2846 1677 210
SbfI CC_TGCA'GG
Cuts at: 1780 1780
Size: 4733
ScaI AGT'ACT
Cuts at: 4250 4250
Size: 4733
SfcI C'TryA_G
Cuts at: 33 290 1185 1776 2461 3454 3645 33
Size: 257 895 591 685 993 191 1121
Fragments arranged by size:
1121 993 895 685 591 257 191
SfoI GGC'GCC
Cuts at: 227 2165 2413 227
Size: 1938 248 2547
Fragments arranged by size:
2547 1938 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
SmaI CCC'GGG
Cuts at: 160 1755 160
Size: 1595 3138
SmlI C'TyrA_G
Cuts at: 497 1126 1888 2803 3295 3557 497
Size: 629 762 915 492 262 1673
Fragments arranged by size:
1673 915 762 629 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4733
SphI G_CATG'C
Cuts at: 1140 1786 2286 1140
Size: 646 500 3587
Fragments arranged by size:
3587 646 500
StyI C'CwwG_G
Cuts at: 1420 1867 2221 1420
Size: 447 354 3932
Fragments arranged by size:
3932 447 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1095 2316 2449 2908 3117 1095
Size: 1221 133 459 209 2711
Fragments arranged by size:
2711 1221 459 209 133
TatI w'GTAC_w
Cuts at: 4248 4248
Size: 4733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4250 4282 4250
Size: 32 4701
Tth111I GACn'n_nGTC
Cuts at: 2530 2530
Size: 4733
XbaI T'CTAG_A
Cuts at: 1441 1764 1441
Size: 323 4410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2228 2228
Size: 4733
XhoI C'TCGA_G
Cuts at: 497 497
Size: 4733
XmaI C'CCGG_G
Cuts at: 158 1753 158
Size: 1595 3138
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AhdI AlfI AloI AscI AvrII BaeI BbvCI
BglI BmtI BpmI BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI