(Circular) (Six-base) MAPSORT of: pF1KB5941.seq Check: 1012 from: 1 to: 4637 pF1KB5941 4637 bp With 182 enzymes: SgfI * October 7, 2009 14:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1098 1098 Size: 4637 AatII G_ACGT'C Cuts at: 634 659 634 Size: 25 4612 AccI GT'mk_AC Cuts at: 1675 1675 Size: 4637 Acc65I G'GTAC_C Cuts at: 1653 1653 Size: 4637 AclI AA'CG_TT Cuts at: 4477 4477 Size: 4637 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 961 2462 2894 3641 3909 961 Size: 1501 432 747 268 1689 Fragments arranged by size: 1689 1501 747 432 268 AfeI AGC'GCT Cuts at: 315 1117 315 Size: 802 3835 AflIII A'CryG_T Cuts at: 446 1989 3093 4070 446 Size: 1543 1104 977 1013 Fragments arranged by size: 1543 1104 1013 977 AgeI A'CCGG_T Cuts at: 3025 3025 Size: 4637 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1338 1338 Size: 4637 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 193 225 193 Size: 32 4605 AlwNI CAG_nnn'CTG Cuts at: 1270 1597 1942 3509 1270 Size: 327 345 1567 2398 Fragments arranged by size: 2398 1567 345 327 ApaI G_GGCC'C Cuts at: 248 328 1168 248 Size: 80 840 3717 Fragments arranged by size: 3717 840 80 ApaLI G'TGCA_C Cuts at: 3407 3407 Size: 4637 ApoI r'AATT_y Cuts at: 1641 3990 4246 1641 Size: 2349 256 2032 Fragments arranged by size: 2349 2032 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4637 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4637 AvaI C'yCGr_G Cuts at: 118 239 327 355 966 1657 118 Size: 121 88 28 611 691 3098 Fragments arranged by size: 3098 691 611 121 88 28 BamHI G'GATC_C Cuts at: 1662 1662 Size: 4637 BanI G'GyrC_C Cuts at: 180 1653 2067 2315 2350 180 Size: 1473 414 248 35 2467 Fragments arranged by size: 2467 1473 414 248 35 BanII G_rGCy'C Cuts at: 248 328 939 973 1063 1168 1536 1651 2681 Size: 80 611 34 90 105 368 115 1030 Cuts at: 2681 248 Size: 2204 Fragments arranged by size: 2204 1030 611 368 115 105 90 80 34 BbeI G_GCGC'C Cuts at: 184 2071 2319 184 Size: 1887 248 2502 Fragments arranged by size: 2502 1887 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 441 475 441 Size: 34 4603 BciVI GTATCCnnnnn_n' Cuts at: 1197 2535 3296 4637 1197 Size: 1338 761 1341 1197 Fragments arranged by size: 1341 1338 1197 761 BclI T'GATC_A Cuts at: 2157 2157 Size: 4637 BglI GCCn_nnn'nGGC Cuts at: 178 232 178 Size: 54 4583 BglII A'GATC_T Cuts at: 950 2152 4189 950 Size: 1202 2037 1398 Fragments arranged by size: 2037 1398 1202 BlpI GC'TnA_GC Cuts at: 727 782 1598 1749 727 Size: 55 816 151 3615 Fragments arranged by size: 3615 816 151 55 Bme1580I G_kGCm'C Cuts at: 248 328 464 1168 1435 2262 2355 3411 248 Size: 80 136 704 267 827 93 1056 1474 Fragments arranged by size: 1474 1056 827 704 267 136 93 80 BmgBI CAC'GTC Cuts at: 449 449 Size: 4637 BmrI ACTGGGnnnn_n' Cuts at: 1244 1849 1930 2265 4409 1244 Size: 605 81 335 2144 1472 Fragments arranged by size: 2144 1472 605 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1380 3004 1380 Size: 1624 3013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 724 825 1195 724 Size: 101 370 4166 Fragments arranged by size: 4166 370 101 Bpu10I CC'TnA_GC Cuts at: 1436 1515 1436 Size: 79 4558 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1813 2692 3184 3482 1813 Size: 879 492 298 2968 Fragments arranged by size: 2968 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4341 4341 Size: 4637 BsaAI yAC'GTr Cuts at: 591 1990 591 Size: 1399 3238 BsaBI GATnn'nnATC Cuts at: 6 1208 2151 4188 6 Size: 1202 943 2037 455 Fragments arranged by size: 2037 1202 943 455 BsaHI Gr'CG_yC Cuts at: 124 181 545 631 656 838 2068 2316 3018 Size: 57 364 86 25 182 1230 248 702 Cuts at: 3018 4514 124 Size: 1496 247 Fragments arranged by size: 1496 1230 702 364 248 247 182 86 57 25 BsaWI w'CCGG_w Cuts at: 1826 2347 3025 3299 3446 3857 1826 Size: 521 678 274 147 411 2606 Fragments arranged by size: 2606 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 328 358 811 841 1387 1417 328 Size: 30 453 30 546 30 3548 Fragments arranged by size: 3548 546 453 30 30 30 BseYI C'CCAG_C Cuts at: 166 316 1121 1348 1399 1406 1590 1612 2062 Size: 150 805 227 51 7 184 22 450 Cuts at: 2062 2820 3397 3893 166 Size: 758 577 496 910 Fragments arranged by size: 910 805 758 577 496 450 227 184 150 51 22 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 370 1050 370 Size: 680 3957 BsiEI CG_ry'CG Cuts at: 104 665 2179 2225 3433 104 Size: 561 1514 46 1208 1308 Fragments arranged by size: 1514 1308 1208 561 46 BsiHKAI G_wGCw'C Cuts at: 539 914 1651 2429 2619 3411 539 Size: 375 737 778 190 792 1765 Fragments arranged by size: 1765 792 778 737 375 190 BsmBI CGTCTCn'nnnn_ Cuts at: 638 663 638 Size: 25 4612 Bsp1286I G_dGCh'C Cuts at: 248 328 464 539 914 939 973 1063 1168 Size: 80 136 75 375 25 34 90 105 Cuts at: 1168 1435 1536 1651 2262 2355 2429 2619 2681 Size: 267 101 115 611 93 74 190 62 Cuts at: 2681 3411 248 Size: 730 1474 Fragments arranged by size: 1474 730 611 375 267 190 136 115 105 101 93 90 80 75 74 62 34 25 BspEI T'CCGG_A Cuts at: 3857 3857 Size: 4637 BspHI T'CATG_A Cuts at: 3813 3813 Size: 4637 BspMI ACCTGCnnnn'nnnn_ Cuts at: 743 1098 1687 1901 2203 2584 743 Size: 355 589 214 302 381 2796 Fragments arranged by size: 2796 589 381 355 302 214 BsrBI CCG'CTC Cuts at: 50 273 584 2929 2983 4467 4632 50 Size: 223 311 2345 54 1484 165 55 Fragments arranged by size: 2345 1484 311 223 165 55 54 BsrDI GCAATG_nn' Cuts at: 2549 2549 Size: 4637 BsrFI r'CCGG_y Cuts at: 664 2635 2816 3025 664 Size: 1971 181 209 2276 Fragments arranged by size: 2276 1971 209 181 BssHII G'CGCG_C Cuts at: 770 770 Size: 4637 BssSI C'ACGA_G Cuts at: 404 883 2908 3266 404 Size: 479 2025 358 1775 Fragments arranged by size: 2025 1775 479 358 BstAPI GCAn_nnn'nTGC Cuts at: 2014 2014 Size: 4637 BstBI TT'CG_AA Cuts at: 2998 2998 Size: 4637 BstXI CCAn_nnnn'nTGG Cuts at: 1042 1620 1042 Size: 578 4059 BstYI r'GATC_y Cuts at: 1 698 950 1662 2129 2152 2487 2733 3745 Size: 697 252 712 467 23 335 246 1012 Cuts at: 3745 3831 3843 4189 1 Size: 86 12 346 449 Fragments arranged by size: 1012 712 697 467 449 346 335 252 246 86 23 12 BtgI C'CryG_G Cuts at: 107 367 398 551 1295 1420 107 Size: 260 31 153 744 125 3324 Fragments arranged by size: 3324 744 260 153 125 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2587 2768 2931 2587 Size: 181 163 4293 Fragments arranged by size: 4293 181 163 BtsI GCAGTG_nn' Cuts at: 1145 1194 1284 1880 1145 Size: 49 90 596 3902 Fragments arranged by size: 3902 596 90 49 DraI TTT'AAA Cuts at: 1636 1636 Size: 4637 DraIII CAC_nnn'GTG Cuts at: 594 719 594 Size: 125 4512 DrdI GACnn_nn'nnGTC Cuts at: 2343 3201 2343 Size: 858 3779 EaeI y'GGCC_r Cuts at: 585 662 1292 2222 2396 2787 2814 4159 585 Size: 77 630 930 174 391 27 1345 1063 Fragments arranged by size: 1345 1063 930 630 391 174 77 27 EagI C'GGCC_G Cuts at: 662 2222 662 Size: 1560 3077 EarI CTCTTCn'nnn_ Cuts at: 245 1000 2660 2870 245 Size: 755 1660 210 2012 Fragments arranged by size: 2012 1660 755 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2845 3155 3301 4447 2845 Size: 310 146 1146 3035 Fragments arranged by size: 3035 1146 310 146 EcoICRI GAG'CTC Cuts at: 1649 1649 Size: 4637 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 724 825 961 1195 2462 2894 3641 3909 724 Size: 101 136 234 1267 432 747 268 1452 Fragments arranged by size: 1452 1267 747 432 268 234 136 101 EcoNI CCTnn'n_nnAGG Cuts at: 388 388 Size: 4637 EcoO109I rG'GnC_Cy Cuts at: 244 1083 1164 1165 1257 1267 1776 244 Size: 839 81 1 92 10 509 3105 Fragments arranged by size: 3105 839 509 92 81 10 1 EcoRI G'AATT_C Cuts at: 1641 1641 Size: 4637 EcoRV GAT'ATC Cuts at: 505 505 Size: 4637 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1032 2656 1032 Size: 1624 3013 FspI TGC'GCA Cuts at: 2418 2418 Size: 4637 HaeII r_GCGC'y Cuts at: 184 317 497 812 1119 2071 2319 3341 4320 Size: 133 180 315 307 952 248 1022 979 Cuts at: 4320 184 Size: 501 Fragments arranged by size: 1022 979 952 501 315 307 248 180 133 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 811 843 1291 1323 1387 1419 1498 1530 2479 Size: 32 448 32 64 32 79 32 949 Cuts at: 2479 2509 2511 2541 811 Size: 30 2 30 2907 Fragments arranged by size: 2907 949 448 79 64 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1676 1676 Size: 4637 KasI G'GCGC_C Cuts at: 180 2067 2315 180 Size: 1887 248 2502 Fragments arranged by size: 2502 1887 248 KpnI G_GTAC'C Cuts at: 1657 1657 Size: 4637 MluI A'CGCG_T Cuts at: 4070 4070 Size: 4637 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 257 797 1589 3308 3492 257 Size: 540 792 1719 184 1402 Fragments arranged by size: 1719 1402 792 540 184 MscI TGG'CCA Cuts at: 1294 2398 1294 Size: 1104 3533 MslI CAynn'nnrTG Cuts at: 524 883 895 2626 2753 524 Size: 359 12 1731 127 2408 Fragments arranged by size: 2408 1731 359 127 12 MspA1I CmG'CkG Cuts at: 1023 1597 1748 2062 2422 3435 3680 1023 Size: 574 151 314 360 1013 245 1980 Fragments arranged by size: 1980 1013 574 360 314 245 151 NaeI GCC'GGC Cuts at: 666 2818 666 Size: 2152 2485 NarI GG'CG_CC Cuts at: 181 2068 2316 181 Size: 1887 248 2502 Fragments arranged by size: 2502 1887 248 NcoI C'CATG_G Cuts at: 107 367 1295 1420 107 Size: 260 928 125 3324 Fragments arranged by size: 3324 928 260 125 NgoMIV G'CCGG_C Cuts at: 664 2816 664 Size: 2152 2485 NspI r_CATG'y Cuts at: 1198 1690 2190 3097 1198 Size: 492 500 907 2738 Fragments arranged by size: 2738 907 500 492 PasI CC'CwG_GG Cuts at: 1264 1527 1264 Size: 263 4374 PciI A'CATG_T Cuts at: 3093 3093 Size: 4637 PflMI CCAn_nnn'nTGG Cuts at: 1270 1270 Size: 4637 PfoI T'CCnGG_A Cuts at: 197 701 1391 197 Size: 504 690 3443 Fragments arranged by size: 3443 690 504 PmeI GTTT'AAAC Cuts at: 1636 1636 Size: 4637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 3776 Size: 32 4605 PpuMI rG'GwC_Cy Cuts at: 1083 1257 1083 Size: 174 4463 PspOMI G'GGCC_C Cuts at: 244 324 1164 244 Size: 80 840 3717 Fragments arranged by size: 3717 840 80 PspXI vC'TCGA_Gb Cuts at: 355 355 Size: 4637 PstI C_TGCA'G Cuts at: 1684 2369 1684 Size: 685 3952 PvuI CG_AT'CG Cuts at: 104 104 Size: 4637 PvuII CAG'CTG Cuts at: 1023 2062 2422 1023 Size: 1039 360 3238 Fragments arranged by size: 3238 1039 360 RsrII CG'GwC_CG Cuts at: 2832 2832 Size: 4637 SacI G_AGCT'C Cuts at: 1651 1651 Size: 4637 SalI G'TCGA_C Cuts at: 1674 1674 Size: 4637 SapI GCTCTTCn'nnn_ Cuts at: 245 2660 2870 245 Size: 2415 210 2012 Fragments arranged by size: 2415 2012 210 SbfI CC_TGCA'GG Cuts at: 1684 1684 Size: 4637 ScaI AGT'ACT Cuts at: 4154 4154 Size: 4637 SfcI C'TryA_G Cuts at: 33 336 437 1509 1680 2365 3358 3549 33 Size: 303 101 1072 171 685 993 191 1121 Fragments arranged by size: 1121 1072 993 685 303 191 171 101 SfoI GGC'GCC Cuts at: 182 2069 2317 182 Size: 1887 248 2502 Fragments arranged by size: 2502 1887 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4637 SmaI CCC'GGG Cuts at: 241 329 1659 241 Size: 88 1330 3219 Fragments arranged by size: 3219 1330 88 SmlI C'TyrA_G Cuts at: 355 1792 2707 3199 3461 355 Size: 1437 915 492 262 1531 Fragments arranged by size: 1531 1437 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4637 SphI G_CATG'C Cuts at: 1198 1690 2190 1198 Size: 492 500 3645 Fragments arranged by size: 3645 500 492 SrfI GCCC'GGGC Cuts at: 329 329 Size: 4637 StuI AGG'CCT Cuts at: 992 992 Size: 4637 StyI C'CwwG_G Cuts at: 107 367 1295 1420 1536 1771 2125 107 Size: 260 928 125 116 235 354 2619 Fragments arranged by size: 2619 928 354 260 235 125 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 639 1018 2220 2353 2812 3021 639 Size: 379 1202 133 459 209 2255 Fragments arranged by size: 2255 1202 459 379 209 133 TatI w'GTAC_w Cuts at: 1382 4152 1382 Size: 2770 1867 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 274 306 4154 4186 274 Size: 32 3848 32 725 Fragments arranged by size: 3848 725 32 32 Tth111I GACn'n_nGTC Cuts at: 2434 2434 Size: 4637 XbaI T'CTAG_A Cuts at: 1668 1668 Size: 4637 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2132 2132 Size: 4637 XhoI C'TCGA_G Cuts at: 355 355 Size: 4637 XmaI C'CCGG_G Cuts at: 239 327 1657 239 Size: 88 1330 3219 Fragments arranged by size: 3219 1330 88 XmnI GAAnn'nnTTC Cuts at: 254 254 Size: 4637 ZraI GAC'GTC Cuts at: 632 657 632 Size: 25 4612 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AflII AhdI AleI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI BseRI BsiWI BsmI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI SwaI