Restriction map for pF1KB5941
 (Circular) (Six-base) MAPSORT of: pF1KB5941.seq  Check: 1012  from: 1  to: 4637



pF1KB5941  4637 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1098    1098 
   Size:       4637
 
AatII G_ACGT'C

Cuts at:    634     659     634 
   Size:         25    4612
 
AccI GT'mk_AC

Cuts at:   1675    1675 
   Size:       4637
 
Acc65I G'GTAC_C

Cuts at:   1653    1653 
   Size:       4637
 
AclI AA'CG_TT

Cuts at:   4477    4477 
   Size:       4637
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    961    2462    2894    3641    3909     961 
   Size:       1501     432     747     268    1689

  Fragments arranged by size: 

                1689    1501     747     432     268
 
AfeI AGC'GCT

Cuts at:    315    1117     315 
   Size:        802    3835
 
AflIII A'CryG_T

Cuts at:    446    1989    3093    4070     446 
   Size:       1543    1104     977    1013

  Fragments arranged by size: 

                1543    1104    1013     977
 
AgeI A'CCGG_T

Cuts at:   3025    3025 
   Size:       4637
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1338    1338 
   Size:       4637
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    193     225     193 
   Size:         32    4605
 
AlwNI CAG_nnn'CTG

Cuts at:   1270    1597    1942    3509    1270 
   Size:        327     345    1567    2398

  Fragments arranged by size: 

                2398    1567     345     327
 
ApaI G_GGCC'C

Cuts at:    248     328    1168     248 
   Size:         80     840    3717

  Fragments arranged by size: 

                3717     840      80
 
ApaLI G'TGCA_C

Cuts at:   3407    3407 
   Size:       4637
 
ApoI r'AATT_y

Cuts at:   1641    3990    4246    1641 
   Size:       2349     256    2032

  Fragments arranged by size: 

                2349    2032     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4637
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4637
 
AvaI C'yCGr_G

Cuts at:    118     239     327     355     966    1657     118 
   Size:        121      88      28     611     691    3098

  Fragments arranged by size: 

                3098     691     611     121      88      28
 
BamHI G'GATC_C

Cuts at:   1662    1662 
   Size:       4637
 
BanI G'GyrC_C

Cuts at:    180    1653    2067    2315    2350     180 
   Size:       1473     414     248      35    2467

  Fragments arranged by size: 

                2467    1473     414     248      35
 
BanII G_rGCy'C

Cuts at:    248     328     939     973    1063    1168    1536    1651    2681 
   Size:         80     611      34      90     105     368     115    1030

Cuts at:   2681     248 
   Size:       2204

  Fragments arranged by size: 

                2204    1030     611     368     115     105      90      80
                  34
 
BbeI G_GCGC'C

Cuts at:    184    2071    2319     184 
   Size:       1887     248    2502

  Fragments arranged by size: 

                2502    1887     248
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    441     475     441 
   Size:         34    4603
 
BciVI GTATCCnnnnn_n'

Cuts at:   1197    2535    3296    4637    1197 
   Size:       1338     761    1341    1197

  Fragments arranged by size: 

                1341    1338    1197     761
 
BclI T'GATC_A

Cuts at:   2157    2157 
   Size:       4637
 
BglI GCCn_nnn'nGGC

Cuts at:    178     232     178 
   Size:         54    4583
 
BglII A'GATC_T

Cuts at:    950    2152    4189     950 
   Size:       1202    2037    1398

  Fragments arranged by size: 

                2037    1398    1202
 
BlpI GC'TnA_GC

Cuts at:    727     782    1598    1749     727 
   Size:         55     816     151    3615

  Fragments arranged by size: 

                3615     816     151      55
 
Bme1580I G_kGCm'C

Cuts at:    248     328     464    1168    1435    2262    2355    3411     248 
   Size:         80     136     704     267     827      93    1056    1474

  Fragments arranged by size: 

                1474    1056     827     704     267     136      93      80
            
 
BmgBI CAC'GTC

Cuts at:    449     449 
   Size:       4637
 
BmrI ACTGGGnnnn_n'

Cuts at:   1244    1849    1930    2265    4409    1244 
   Size:        605      81     335    2144    1472

  Fragments arranged by size: 

                2144    1472     605     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1380    3004    1380 
   Size:       1624    3013
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    724     825    1195     724 
   Size:        101     370    4166

  Fragments arranged by size: 

                4166     370     101
 
Bpu10I CC'TnA_GC

Cuts at:   1436    1515    1436 
   Size:         79    4558
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1813    2692    3184    3482    1813 
   Size:        879     492     298    2968

  Fragments arranged by size: 

                2968     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4341    4341 
   Size:       4637
 
BsaAI yAC'GTr

Cuts at:    591    1990     591 
   Size:       1399    3238
 
BsaBI GATnn'nnATC

Cuts at:      6    1208    2151    4188       6 
   Size:       1202     943    2037     455

  Fragments arranged by size: 

                2037    1202     943     455
 
BsaHI Gr'CG_yC

Cuts at:    124     181     545     631     656     838    2068    2316    3018 
   Size:         57     364      86      25     182    1230     248     702

Cuts at:   3018    4514     124 
   Size:       1496     247

  Fragments arranged by size: 

                1496    1230     702     364     248     247     182      86
                  57      25
 
BsaWI w'CCGG_w

Cuts at:   1826    2347    3025    3299    3446    3857    1826 
   Size:        521     678     274     147     411    2606

  Fragments arranged by size: 

                2606     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    328     358     811     841    1387    1417     328 
   Size:         30     453      30     546      30    3548

  Fragments arranged by size: 

                3548     546     453      30      30      30
 
BseYI C'CCAG_C

Cuts at:    166     316    1121    1348    1399    1406    1590    1612    2062 
   Size:        150     805     227      51       7     184      22     450

Cuts at:   2062    2820    3397    3893     166 
   Size:        758     577     496     910

  Fragments arranged by size: 

                 910     805     758     577     496     450     227     184
                 150      51      22       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    370    1050     370 
   Size:        680    3957
 
BsiEI CG_ry'CG

Cuts at:    104     665    2179    2225    3433     104 
   Size:        561    1514      46    1208    1308

  Fragments arranged by size: 

                1514    1308    1208     561      46
 
BsiHKAI G_wGCw'C

Cuts at:    539     914    1651    2429    2619    3411     539 
   Size:        375     737     778     190     792    1765

  Fragments arranged by size: 

                1765     792     778     737     375     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    638     663     638 
   Size:         25    4612
 
Bsp1286I G_dGCh'C

Cuts at:    248     328     464     539     914     939     973    1063    1168 
   Size:         80     136      75     375      25      34      90     105

Cuts at:   1168    1435    1536    1651    2262    2355    2429    2619    2681 
   Size:        267     101     115     611      93      74     190      62

Cuts at:   2681    3411     248 
   Size:        730    1474

  Fragments arranged by size: 

                1474     730     611     375     267     190     136     115
                 105     101      93      90      80      75      74      62
                  34      25
 
BspEI T'CCGG_A

Cuts at:   3857    3857 
   Size:       4637
 
BspHI T'CATG_A

Cuts at:   3813    3813 
   Size:       4637
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    743    1098    1687    1901    2203    2584     743 
   Size:        355     589     214     302     381    2796

  Fragments arranged by size: 

                2796     589     381     355     302     214
 
BsrBI CCG'CTC

Cuts at:     50     273     584    2929    2983    4467    4632      50 
   Size:        223     311    2345      54    1484     165      55

  Fragments arranged by size: 

                2345    1484     311     223     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2549    2549 
   Size:       4637
 
BsrFI r'CCGG_y

Cuts at:    664    2635    2816    3025     664 
   Size:       1971     181     209    2276

  Fragments arranged by size: 

                2276    1971     209     181
 
BssHII G'CGCG_C

Cuts at:    770     770 
   Size:       4637
 
BssSI C'ACGA_G

Cuts at:    404     883    2908    3266     404 
   Size:        479    2025     358    1775

  Fragments arranged by size: 

                2025    1775     479     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2014    2014 
   Size:       4637
 
BstBI TT'CG_AA

Cuts at:   2998    2998 
   Size:       4637
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1042    1620    1042 
   Size:        578    4059
 
BstYI r'GATC_y

Cuts at:      1     698     950    1662    2129    2152    2487    2733    3745 
   Size:        697     252     712     467      23     335     246    1012

Cuts at:   3745    3831    3843    4189       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     712     697     467     449     346     335     252
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     367     398     551    1295    1420     107 
   Size:        260      31     153     744     125    3324

  Fragments arranged by size: 

                3324     744     260     153     125      31
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2587    2768    2931    2587 
   Size:        181     163    4293

  Fragments arranged by size: 

                4293     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1145    1194    1284    1880    1145 
   Size:         49      90     596    3902

  Fragments arranged by size: 

                3902     596      90      49
 
DraI TTT'AAA

Cuts at:   1636    1636 
   Size:       4637
 
DraIII CAC_nnn'GTG

Cuts at:    594     719     594 
   Size:        125    4512
 
DrdI GACnn_nn'nnGTC

Cuts at:   2343    3201    2343 
   Size:        858    3779
 
EaeI y'GGCC_r

Cuts at:    585     662    1292    2222    2396    2787    2814    4159     585 
   Size:         77     630     930     174     391      27    1345    1063

  Fragments arranged by size: 

                1345    1063     930     630     391     174      77      27
            
 
EagI C'GGCC_G

Cuts at:    662    2222     662 
   Size:       1560    3077
 
EarI CTCTTCn'nnn_

Cuts at:    245    1000    2660    2870     245 
   Size:        755    1660     210    2012

  Fragments arranged by size: 

                2012    1660     755     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2845    3155    3301    4447    2845 
   Size:        310     146    1146    3035

  Fragments arranged by size: 

                3035    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1649    1649 
   Size:       4637
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    724     825     961    1195    2462    2894    3641    3909     724 
   Size:        101     136     234    1267     432     747     268    1452

  Fragments arranged by size: 

                1452    1267     747     432     268     234     136     101
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    388     388 
   Size:       4637
 
EcoO109I rG'GnC_Cy

Cuts at:    244    1083    1164    1165    1257    1267    1776     244 
   Size:        839      81       1      92      10     509    3105

  Fragments arranged by size: 

                3105     839     509      92      81      10       1
 
EcoRI G'AATT_C

Cuts at:   1641    1641 
   Size:       4637
 
EcoRV GAT'ATC

Cuts at:    505     505 
   Size:       4637
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1032    2656    1032 
   Size:       1624    3013
 
FspI TGC'GCA

Cuts at:   2418    2418 
   Size:       4637
 
HaeII r_GCGC'y

Cuts at:    184     317     497     812    1119    2071    2319    3341    4320 
   Size:        133     180     315     307     952     248    1022     979

Cuts at:   4320     184 
   Size:        501

  Fragments arranged by size: 

                1022     979     952     501     315     307     248     180
                 133
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    811     843    1291    1323    1387    1419    1498    1530    2479 
   Size:         32     448      32      64      32      79      32     949

Cuts at:   2479    2509    2511    2541     811 
   Size:         30       2      30    2907

  Fragments arranged by size: 

                2907     949     448      79      64      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   1676    1676 
   Size:       4637
 
KasI G'GCGC_C

Cuts at:    180    2067    2315     180 
   Size:       1887     248    2502

  Fragments arranged by size: 

                2502    1887     248
 
KpnI G_GTAC'C

Cuts at:   1657    1657 
   Size:       4637
 
MluI A'CGCG_T

Cuts at:   4070    4070 
   Size:       4637
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    257     797    1589    3308    3492     257 
   Size:        540     792    1719     184    1402

  Fragments arranged by size: 

                1719    1402     792     540     184
 
MscI TGG'CCA

Cuts at:   1294    2398    1294 
   Size:       1104    3533
 
MslI CAynn'nnrTG

Cuts at:    524     883     895    2626    2753     524 
   Size:        359      12    1731     127    2408

  Fragments arranged by size: 

                2408    1731     359     127      12
 
MspA1I CmG'CkG

Cuts at:   1023    1597    1748    2062    2422    3435    3680    1023 
   Size:        574     151     314     360    1013     245    1980

  Fragments arranged by size: 

                1980    1013     574     360     314     245     151
 
NaeI GCC'GGC

Cuts at:    666    2818     666 
   Size:       2152    2485
 
NarI GG'CG_CC

Cuts at:    181    2068    2316     181 
   Size:       1887     248    2502

  Fragments arranged by size: 

                2502    1887     248
 
NcoI C'CATG_G

Cuts at:    107     367    1295    1420     107 
   Size:        260     928     125    3324

  Fragments arranged by size: 

                3324     928     260     125
 
NgoMIV G'CCGG_C

Cuts at:    664    2816     664 
   Size:       2152    2485
 
NspI r_CATG'y

Cuts at:   1198    1690    2190    3097    1198 
   Size:        492     500     907    2738

  Fragments arranged by size: 

                2738     907     500     492
 
PasI CC'CwG_GG

Cuts at:   1264    1527    1264 
   Size:        263    4374
 
PciI A'CATG_T

Cuts at:   3093    3093 
   Size:       4637
 
PflMI CCAn_nnn'nTGG

Cuts at:   1270    1270 
   Size:       4637
 
PfoI T'CCnGG_A

Cuts at:    197     701    1391     197 
   Size:        504     690    3443

  Fragments arranged by size: 

                3443     690     504
 
PmeI GTTT'AAAC

Cuts at:   1636    1636 
   Size:       4637
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3776    3808    3776 
   Size:         32    4605
 
PpuMI rG'GwC_Cy

Cuts at:   1083    1257    1083 
   Size:        174    4463
 
PspOMI G'GGCC_C

Cuts at:    244     324    1164     244 
   Size:         80     840    3717

  Fragments arranged by size: 

                3717     840      80
 
PspXI vC'TCGA_Gb

Cuts at:    355     355 
   Size:       4637
 
PstI C_TGCA'G

Cuts at:   1684    2369    1684 
   Size:        685    3952
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4637
 
PvuII CAG'CTG

Cuts at:   1023    2062    2422    1023 
   Size:       1039     360    3238

  Fragments arranged by size: 

                3238    1039     360
 
RsrII CG'GwC_CG

Cuts at:   2832    2832 
   Size:       4637
 
SacI G_AGCT'C

Cuts at:   1651    1651 
   Size:       4637
 
SalI G'TCGA_C

Cuts at:   1674    1674 
   Size:       4637
 
SapI GCTCTTCn'nnn_

Cuts at:    245    2660    2870     245 
   Size:       2415     210    2012

  Fragments arranged by size: 

                2415    2012     210
 
SbfI CC_TGCA'GG

Cuts at:   1684    1684 
   Size:       4637
 
ScaI AGT'ACT

Cuts at:   4154    4154 
   Size:       4637
 
SfcI C'TryA_G

Cuts at:     33     336     437    1509    1680    2365    3358    3549      33 
   Size:        303     101    1072     171     685     993     191    1121

  Fragments arranged by size: 

                1121    1072     993     685     303     191     171     101
            
 
SfoI GGC'GCC

Cuts at:    182    2069    2317     182 
   Size:       1887     248    2502

  Fragments arranged by size: 

                2502    1887     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4637
 
SmaI CCC'GGG

Cuts at:    241     329    1659     241 
   Size:         88    1330    3219

  Fragments arranged by size: 

                3219    1330      88
 
SmlI C'TyrA_G

Cuts at:    355    1792    2707    3199    3461     355 
   Size:       1437     915     492     262    1531

  Fragments arranged by size: 

                1531    1437     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4637
 
SphI G_CATG'C

Cuts at:   1198    1690    2190    1198 
   Size:        492     500    3645

  Fragments arranged by size: 

                3645     500     492
 
SrfI GCCC'GGGC

Cuts at:    329     329 
   Size:       4637
 
StuI AGG'CCT

Cuts at:    992     992 
   Size:       4637
 
StyI C'CwwG_G

Cuts at:    107     367    1295    1420    1536    1771    2125     107 
   Size:        260     928     125     116     235     354    2619

  Fragments arranged by size: 

                2619     928     354     260     235     125     116
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    639    1018    2220    2353    2812    3021     639 
   Size:        379    1202     133     459     209    2255

  Fragments arranged by size: 

                2255    1202     459     379     209     133
 
TatI w'GTAC_w

Cuts at:   1382    4152    1382 
   Size:       2770    1867
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    274     306    4154    4186     274 
   Size:         32    3848      32     725

  Fragments arranged by size: 

                3848     725      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2434    2434 
   Size:       4637
 
XbaI T'CTAG_A

Cuts at:   1668    1668 
   Size:       4637
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2132    2132 
   Size:       4637
 
XhoI C'TCGA_G

Cuts at:    355     355 
   Size:       4637
 
XmaI C'CCGG_G

Cuts at:    239     327    1657     239 
   Size:         88    1330    3219

  Fragments arranged by size: 

                3219    1330      88
 
XmnI GAAnn'nnTTC

Cuts at:    254     254 
   Size:       4637
 
ZraI GAC'GTC

Cuts at:    632     657     632 
   Size:         25    4612
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AhdI     AleI     AscI    AvrII     BaeI     BbsI    BbvCI
    BfrBI     BmtI    BseRI    BsiWI     BsmI    BsrGI   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PmlI    PshAI
     PsiI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SspI     SwaI
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