Restriction map for
pF1KB5947
(Circular) (Six-base) MAPSORT of: pF1KB5947.seq Check: 6643 from: 1 to: 4640
pF1KB5947 4640 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1678 1678
Size: 4640
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 4480 4480
Size: 4640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 862 952 1085 2465 2897 3644 3912 728
Size: 134 90 133 1380 432 747 268 1456
Fragments arranged by size:
1456 1380 747 432 268 134 133 90
AflIII A'CryG_T
Cuts at: 1992 3096 4073 1992
Size: 1104 977 2559
Fragments arranged by size:
2559 1104 977
AgeI A'CCGG_T
Cuts at: 3028 3028
Size: 4640
AleI CACnn'nnGTG
Cuts at: 913 913
Size: 4640
AlwNI CAG_nnn'CTG
Cuts at: 414 531 1005 1464 1945 3512 414
Size: 117 474 459 481 1567 1542
Fragments arranged by size:
1567 1542 481 474 459 117
ApaLI G'TGCA_C
Cuts at: 756 3410 756
Size: 2654 1986
ApoI r'AATT_y
Cuts at: 1644 3993 4249 1644
Size: 2349 256 2035
Fragments arranged by size:
2349 2035 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4640
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 538 1660 538
Size: 1122 3518
BamHI G'GATC_C
Cuts at: 1388 1665 1388
Size: 277 4363
BanI G'GyrC_C
Cuts at: 534 1656 2070 2318 2353 534
Size: 1122 414 248 35 2821
Fragments arranged by size:
2821 1122 414 248 35
BanII G_rGCy'C
Cuts at: 329 479 1040 1654 2684 329
Size: 150 561 614 1030 2285
Fragments arranged by size:
2285 1030 614 561 150
BbeI G_GCGC'C
Cuts at: 538 2074 2322 538
Size: 1536 248 2856
Fragments arranged by size:
2856 1536 248
BbvCI CC'TCA_GC
Cuts at: 1628 1628
Size: 4640
BciVI GTATCCnnnnn_n'
Cuts at: 2538 3299 4640 2538
Size: 761 1341 2538
Fragments arranged by size:
2538 1341 761
BclI T'GATC_A
Cuts at: 2160 2160
Size: 4640
BglI GCCn_nnn'nGGC
Cuts at: 431 964 431
Size: 533 4107
BglII A'GATC_T
Cuts at: 342 2155 4192 342
Size: 1813 2037 790
Fragments arranged by size:
2037 1813 790
BlpI GC'TnA_GC
Cuts at: 1752 1752
Size: 4640
Bme1580I G_kGCm'C
Cuts at: 211 760 908 2265 2358 3414 211
Size: 549 148 1357 93 1056 1437
Fragments arranged by size:
1437 1357 1056 549 148 93
BmrI ACTGGGnnnn_n'
Cuts at: 1852 1933 2268 4412 1852
Size: 81 335 2144 2080
Fragments arranged by size:
2144 2080 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 493 1486 1609 400
Size: 93 993 123 3431
Fragments arranged by size:
3431 993 123 93
Bpu10I CC'TnA_GC
Cuts at: 502 1234 1628 502
Size: 732 394 3514
Fragments arranged by size:
3514 732 394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1816 2695 3187 3485 1075
Size: 741 879 492 298 2230
Fragments arranged by size:
2230 879 741 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4344 4344
Size: 4640
BsaAI yAC'GTr
Cuts at: 1993 1993
Size: 4640
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 535 956 2071 2319 3021 4517 535
Size: 421 1115 248 702 1496 658
Fragments arranged by size:
1496 1115 702 658 421 248
BsaWI w'CCGG_w
Cuts at: 1829 2350 3028 3302 3449 3860 1829
Size: 521 678 274 147 411 2609
Fragments arranged by size:
2609 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 250 280 623 653 802 832 250
Size: 30 343 30 149 30 4058
Fragments arranged by size:
4058 343 149 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1522 1522
Size: 4640
BseYI C'CCAG_C
Cuts at: 858 2065 2823 3400 3896 858
Size: 1207 758 577 496 1602
Fragments arranged by size:
1602 1207 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 298
Size: 4640
BsiEI CG_ry'CG
Cuts at: 104 1419 2182 2228 3436 104
Size: 1315 763 46 1208 1308
Fragments arranged by size:
1315 1308 1208 763 46
BsiHKAI G_wGCw'C
Cuts at: 583 760 1654 2432 2622 3414 583
Size: 177 894 778 190 792 1809
Fragments arranged by size:
1809 894 792 778 190 177
BsmI GAATG_Cn'
Cuts at: 1165 1165
Size: 4640
BsmBI CGTCTCn'nnnn_
Cuts at: 829 829
Size: 4640
Bsp1286I G_dGCh'C
Cuts at: 211 329 479 583 760 908 1040 1654 2265
Size: 118 150 104 177 148 132 614 611
Cuts at: 2265 2358 2432 2622 2684 3414 211
Size: 93 74 190 62 730 1437
Fragments arranged by size:
1437 730 614 611 190 177 150 148
132 118 104 93 74 62
BspEI T'CCGG_A
Cuts at: 3860 3860
Size: 4640
BspHI T'CATG_A
Cuts at: 980 1523 3816 980
Size: 543 2293 1804
Fragments arranged by size:
2293 1804 543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1583 1690 1904 2206 2587 1583
Size: 107 214 302 381 3636
Fragments arranged by size:
3636 381 302 214 107
BsrBI CCG'CTC
Cuts at: 50 389 522 855 1179 2932 2986 4470 4635
Size: 339 133 333 324 1753 54 1484 165
Cuts at: 4635 50
Size: 55
Fragments arranged by size:
1753 1484 339 333 324 165 133 55
54
BsrDI GCAATG_nn'
Cuts at: 2552 2552
Size: 4640
BsrFI r'CCGG_y
Cuts at: 276 1556 2638 2819 3028 276
Size: 1280 1082 181 209 1888
Fragments arranged by size:
1888 1280 1082 209 181
BssSI C'ACGA_G
Cuts at: 2911 3269 2911
Size: 358 4282
BstAPI GCAn_nnn'nTGC
Cuts at: 2017 2017
Size: 4640
BstBI TT'CG_AA
Cuts at: 3001 3001
Size: 4640
BstEII G'GTnAC_C
Cuts at: 252 1026 252
Size: 774 3866
BstXI CCAn_nnnn'nTGG
Cuts at: 1218 1218
Size: 4640
BstYI r'GATC_y
Cuts at: 1 342 653 1388 1665 2132 2155 2490 2736
Size: 341 311 735 277 467 23 335 246
Cuts at: 2736 3748 3834 3846 4192 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 735 467 449 346 341 335 311
277 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 419 584 802 1413 107
Size: 312 165 218 611 3334
Fragments arranged by size:
3334 611 312 218 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2590 2771 2934 2590
Size: 181 163 4296
Fragments arranged by size:
4296 181 163
BtsI GCAGTG_nn'
Cuts at: 286 1587 1883 286
Size: 1301 296 3043
Fragments arranged by size:
3043 1301 296
ClaI AT'CG_AT
Cuts at: 158 158
Size: 4640
DraI TTT'AAA
Cuts at: 1639 1639
Size: 4640
DrdI GACnn_nn'nnGTC
Cuts at: 2346 3204 2346
Size: 858 3782
EaeI y'GGCC_r
Cuts at: 455 965 1416 1604 2225 2399 2790 2817 4162
Size: 510 451 188 621 174 391 27 1345
Cuts at: 4162 455
Size: 933
Fragments arranged by size:
1345 933 621 510 451 391 188 174
27
EagI C'GGCC_G
Cuts at: 1416 2225 1416
Size: 809 3831
EarI CTCTTCn'nnn_
Cuts at: 919 1169 2663 2873 919
Size: 250 1494 210 2686
Fragments arranged by size:
2686 1494 250 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 263 715 2848 3158 3304 4450 263
Size: 452 2133 310 146 1146 453
Fragments arranged by size:
2133 1146 453 452 310 146
EcoICRI GAG'CTC
Cuts at: 1652 1652
Size: 4640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 493 728 862 952 1085 1486 1609 2465
Size: 93 235 134 90 133 401 123 856
Cuts at: 2465 2897 3644 3912 400
Size: 432 747 268 1128
Fragments arranged by size:
1128 856 747 432 401 268 235 134
133 123 93 90
EcoNI CCTnn'n_nnAGG
Cuts at: 934 967 1273 1430 934
Size: 33 306 157 4144
Fragments arranged by size:
4144 306 157 33
EcoO109I rG'GnC_Cy
Cuts at: 243 1779 243
Size: 1536 3104
EcoRI G'AATT_C
Cuts at: 1644 1644
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 4640
FspI TGC'GCA
Cuts at: 1579 2421 1579
Size: 842 3798
HaeII r_GCGC'y
Cuts at: 538 1333 1548 2074 2322 3344 4323 538
Size: 795 215 526 248 1022 979 855
Fragments arranged by size:
1022 979 855 795 526 248 215
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 1363 1395 2482 2512 2514 2544 645
Size: 32 686 32 1087 30 2 30 2741
Fragments arranged by size:
2741 1087 686 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1115 1679 1115
Size: 564 4076
KasI G'GCGC_C
Cuts at: 534 2070 2318 534
Size: 1536 248 2856
Fragments arranged by size:
2856 1536 248
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3311 3495 3311
Size: 184 4456
MscI TGG'CCA
Cuts at: 457 2401 457
Size: 1944 2696
MslI CAynn'nnrTG
Cuts at: 160 320 913 1219 2629 2756 160
Size: 160 593 306 1410 127 2044
Fragments arranged by size:
2044 1410 593 306 160 127
MspA1I CmG'CkG
Cuts at: 586 990 1002 1751 2065 2425 3438 3683 586
Size: 404 12 749 314 360 1013 245 1543
Fragments arranged by size:
1543 1013 749 404 360 314 245 12
NaeI GCC'GGC
Cuts at: 278 2821 278
Size: 2543 2097
NarI GG'CG_CC
Cuts at: 535 2071 2319 535
Size: 1536 248 2856
Fragments arranged by size:
2856 1536 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4640
NgoMIV G'CCGG_C
Cuts at: 276 2819 276
Size: 2543 2097
NspI r_CATG'y
Cuts at: 948 1693 2193 3100 948
Size: 745 500 907 2488
Fragments arranged by size:
2488 907 745 500
PasI CC'CwG_GG
Cuts at: 830 1432 830
Size: 602 4038
PciI A'CATG_T
Cuts at: 3096 3096
Size: 4640
PflMI CCAn_nnn'nTGG
Cuts at: 199 1005 1322 199
Size: 806 317 3517
Fragments arranged by size:
3517 806 317
PfoI T'CCnGG_A
Cuts at: 377 566 608 1130 377
Size: 189 42 522 3887
Fragments arranged by size:
3887 522 189 42
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 4608
PpuMI rG'GwC_Cy
Cuts at: 243 243
Size: 4640
PshAI GACnn'nnGTC
Cuts at: 1499 1499
Size: 4640
PstI C_TGCA'G
Cuts at: 124 1687 2372 124
Size: 1563 685 2392
Fragments arranged by size:
2392 1563 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4640
PvuII CAG'CTG
Cuts at: 990 2065 2425 990
Size: 1075 360 3205
Fragments arranged by size:
3205 1075 360
RsrII CG'GwC_CG
Cuts at: 606 2835 606
Size: 2229 2411
SacI G_AGCT'C
Cuts at: 1654 1654
Size: 4640
SacII CC_GC'GG
Cuts at: 587 587
Size: 4640
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SanDI GG'GwC_CC
Cuts at: 243 243
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 919 1169 2663 2873 919
Size: 250 1494 210 2686
Fragments arranged by size:
2686 1494 250 210
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 4157 4157
Size: 4640
SexAI A'CCwGG_T
Cuts at: 299 299
Size: 4640
SfcI C'TryA_G
Cuts at: 33 120 1683 2368 3361 3552 33
Size: 87 1563 685 993 191 1121
Fragments arranged by size:
1563 1121 993 685 191 87
SfoI GGC'GCC
Cuts at: 536 2072 2320 536
Size: 1536 248 2856
Fragments arranged by size:
2856 1536 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 540 1662 540
Size: 1122 3518
SmlI C'TyrA_G
Cuts at: 1090 1795 2710 3202 3464 1090
Size: 705 915 492 262 2266
Fragments arranged by size:
2266 915 705 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
StyI C'CwwG_G
Cuts at: 107 1774 2128 107
Size: 1667 354 2619
Fragments arranged by size:
2619 1667 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 594 1045 1426 2223 2356 2815 3024 594
Size: 451 381 797 133 459 209 2210
Fragments arranged by size:
2210 797 459 451 381 209 133
TatI w'GTAC_w
Cuts at: 1286 4155 1286
Size: 2869 1771
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 590 622 623 655 802 834 4157 4189 590
Size: 32 1 32 147 32 3323 32 1041
Fragments arranged by size:
3323 1041 147 32 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 2437 2437
Size: 4640
XbaI T'CTAG_A
Cuts at: 1671 1671
Size: 4640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 192 2135 192
Size: 1943 2697
XmaI C'CCGG_G
Cuts at: 538 1660 538
Size: 1122 3518
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsiWI BsrGI BssHII BstZ17I Bsu36I CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI